GenBank-Updates@genbank.bio.net (05/18/91)
LOCUS LISIAP 2046 bp ds-DNA BCT 18-MAY-1991
DEFINITION L. monocytogenes iap gene for protein p60
ACCESSION X52268
KEYWORDS extracellular protein; iap gene; secreted protein.
SOURCE Listeria monocytogenes DNA.
ORGANISM Listeria monocytogenes
Prokaryota; Bacteria; Firmicutes; Regular asporogenous rods;
Lactobacillaceae.
REFERENCE 1 (bases 1 to 2046)
AUTHORS Goebel,W.
JOURNAL Unpublished (1990)
STANDARD full automatic
REFERENCE 2 (bases 1 to 2046)
AUTHORS Koehler,S., Leimeister-Waechter,M., Chakraborty,T., Lottspeich,F.
and Goebel,W.
TITLE The gene coding for protein p60 of Listeria monocytogenes and its
use as a specific probe for Listeria monocytogenes.
JOURNAL Infect. Immun. 58, 1943-1950 (1990)
STANDARD full automatic
COMMENT *source: strain=SV1/2a EGD (Kaufmann); *source: library=pUC18;
clone=pLM37, pSK5;
From EMBL entry LMIAP; dated 15-FEB-1991.
FEATURES Location/Qualifiers
RBS 434..438
/note="ribosome binding site"
CDS 443..1894
/note="precursor polypeptide (AA -27 to 457)"
/codon_start=443
CDS 443..523
/note="signal peptide (AA -27 to -1)"
/codon_start=443
CDS 524..1894
/note="mature p60 protein (AA 1-457)"
/codon_start=524
misc_feature 1373..1507
/note="repeat region"
terminator 1913..1945
/note="terminator"
BASE COUNT 758 a 402 c 350 g 536 t
ORIGIN
1 tcgatcatca taattctgtc tcattatata acatcctcca taccttctat tatagaatac
61 cataaactca tctggcaatt catttcgagt cacgaagaac ggaaaaactg ccggttttta
121 tattacaaat gtattaagtt tttctattaa caaaaaacaa taggtttccc atagcgaaag
181 ttgttgatta acgttcacat cccacttaca ctataaaggt ttacccagca gtacatctca
241 agccctaaga atacacgttc gcttttcaac tgttacagaa ttattacaaa tagttggtat
301 agtcctcttt agcctttgga gttattatct catcatttgt tttttaggtg aaaactgggt
361 aaacttagta ttaatcaata taaaattaat tctcaaatac ttaattacgt actgggattt
421 tctgaaaaaa gagaggagtt ttatgaatat gaaaaaagca actatcgcgg ctacagctgg
481 gattgcggta acagcatttg ctgcgccaac aatcgcatcc gcaagcactg tagtagtcga
541 agctggtgat actctttggg gtatcgcaca aagtaaaggg actactgttg acgcaattaa
601 aaaagcaaac aatttaacaa cagataaaat cgtaccaggt caaaaattac aagtaaataa
661 tgaggttgct gctgctgaaa aaacagagaa atctgttagc gcaacttggt taaacgtccg
721 tagtggcgct ggtgttgata acagtattat tacgtccatc aaaggtggaa caaaagtaac
781 tgttgaaaca accgaatcta acggctggca caaaattact tacaacgatg gaaaaactgg
841 tttcgttaac ggtaaatact taactgacaa agcagtaagc actccagttg caccaacaca
901 agaagtgaaa aaagaaacta ctactcaaca agctgcacct gctgcagaaa caaaaactga
961 agtaaaacaa actacacaag caactacacc tgcgcctaaa gtagcagaaa cgaaagaaac
1021 tccagtagta gatcaaaatg ctactacaca cgctgttaaa agcggtgaca ctatttgggc
1081 tttatccgta aaatacggtg tttctgttca agacattatg tcatggaata atttatcttc
1141 ttcttctatt tatgtaggtc aaaagcttgc tattaaacaa actgctaaca cagctactcc
1201 aaaagcagaa gtgaaaacgg aagctccagc agctgaaaaa caagcagctc cagtagttaa
1261 agaaaatact aacacaaata ctgctactac agagaaaaaa gaaacagcaa cgcaacaaca
1321 aacagcacct aaagcaccaa cagaagctgc aaaaccagct cctgcaccat ctacaaacac
1381 aaatgctaat aaaacaaata caaatacaaa tacaaataca aatacaaaca atactaatac
1441 aaatacacca tctaaaaata ctaatacaaa ctcaaatact aatacgaata caaactcaaa
1501 tacgaatgct aatcaaggtt cttccaacaa taacagcaat tcaagtgcaa gtgctattat
1561 tgctgaagct caaaaacacc ttggaaaagc ttattcatgg ggtggtaacg gaccaactac
1621 atttgattgc tctggttaca ctaaatatgt atttgctaaa gcgggaatct cccttccacg
1681 tacttctggc gcacaatacg ctagcactac aagaatctct gaatctcaag caaaacctgg
1741 tgatttagta ttctttgact atggtagcgg aatttctcac gttggtatct acgttggtaa
1801 tggtcaaatg attaacgcgc aagacaatgg cgttaaatac gataacatcc acggctctgg
1861 ctggggtaaa tatctagttg gcttcggtcg cgtataatta ataacttaaa gtaacctgtg
1921 gagcaagcag ttcgcttcac aggttttttg ttggaaattt tatcttaatg aaagacggtg
1981 tatgatgaag aatctagtaa aagtaaaagt tttcctaagt tcacaaaagg ctataaggaa
2041 ggttat
//