GenBank-Updates@genbank.bio.net (05/18/91)
LOCUS ECO5388 1125 bp ds-DNA BCT 18-MAY-1991
DEFINITION E. coli plasmid-associated dihydrofolate reductase gene (for
trimethoprim resistance)
ACCESSION V00252 J01774
KEYWORDS drug resistance gene; reductase; trimethoprim resistance.
SOURCE Escherichia coli DNA.
ORGANISM Escherichia coli
Prokaryota; Bacteria; Gracilicutes; Scotobacteria;
Facultatively anaerobic rods; Enterobacteriaceae.
REFERENCE 1 (bases 1 to 1125)
AUTHORS Zolg,J.W. and Haenggi,U.J.
TITLE Characterization of a R plasmid-associated, trimethoprim- resistant
dihydrofolate reductase gene and determination of the nucleotide
sequence of the reductase gene
JOURNAL Nucleic Acids Res. 9, 697-710 (1981)
STANDARD full automatic
COMMENT SWISS-PROT; P00384; DYR3$ECOLI.
From EMBL entry EC5388; dated 21-FEB-1991.
FEATURES Location/Qualifiers
CDS 772..1005
/gene="dihydrofolate reductase gene"
/codon_start=772
BASE COUNT 248 a 311 c 321 g 245 t
ORIGIN
1 ggtcccgatc cttggagccc ttgccctccc gcacgatgat cgtgccgtga tcgaaatcca
61 gatccttgac ccgcagttgc aaaccctcac tgatccgcat gcccgttcca tacagaagct
121 gggcgaacaa acgatgctcg ccttccagaa aaccgaggat gcgaaccact tcatccgggg
181 tcagcaccac cggcaagcgc cgcgacggcc gaggtcttcc gatctcctga agccagggca
241 gatccgtgca cagcaccttg ccgtagaaga acagcaaggc cgccaatgcc tgacgatgcg
301 tggagaccga aaccttgcgc tcgttcgcca gccaggacag aaatgcctcg acttcgctgc
361 tgcccaaggt tgccgggtga cgcacaccgt ggaaacggat gaaggcacga acccagttga
421 cataagcctg ttcggttcgt aaactgtaat gcaagtagcg tatgcgctca cgcaactggt
481 ccagaacctt gaccgaacgc agcggtggta acggcgcagt ggcggttttc atggcttgtt
541 atgactgttt ttttgtacag tctagcctcg ggcatccaag ctagctaagc gcgttacgcc
601 gtgggtcgat gtttgatgtt atggaacagc aacgatgtta cgcagcaggg tagtcgccct
661 aaaacaaagt taggcagccg ttgtgctggt gctttctagt agttgttgtg gggtaggcag
721 tcagagttcg atttgcttgt cgccataata gattcacaag aaggattcga catgggtcaa
781 agtagcgatg aagccaacgc tcccgttgca gggcagtttg cgcttcccct gagtgccacc
841 tttggcttag gggatcgcgt acgcaagaaa tctggtgccg cttggcaggg tcaagtcgtc
901 ggttggtatt gcacaaaact cactcctgaa ggctatgcgg tcgagtccga atcccaccca
961 ggctcagtgc aaatttatcc tgtggctgca cttgaacgtg tggcctaaca attcgctcag
1021 gacggttcgc cgcccgctga gctttgtcgt taggcgtcat gggctgacgc tcacaatcga
1081 accaagcggc gagagtcgcg ggcactgcga ctgctgtggg aattc
//