GenBank-Updates@genbank.bio.net (05/18/91)
LOCUS ECOGLNT 2306 bp ds-DNA BCT 18-MAY-1991
DEFINITION E. coli gene glnS for glutaminyl-tRNA synthetase
ACCESSION V01575 M10187 X01735 X01743
KEYWORDS synthetase; trna synthetase.
SOURCE Escherichia coli DNA.
ORGANISM Escherichia coli
Prokaryota; Bacteria; Gracilicutes; Scotobacteria;
Facultatively anaerobic rods; Enterobacteriaceae.
REFERENCE 1 (bases 1 to 2306)
AUTHORS Yamao,F., Inokuchi,H., Cheung,A., Ozeki,H. and Soell,D.G.
TITLE Escherichia coli glutaminyl-tRNA synthetase
JOURNAL J. Biol. Chem. 257, 11639-11643 (1982)
STANDARD full automatic
REFERENCE 2 (sites)
AUTHORS Hoben,P., Uemura,H., Yamao,F., Cheung,A., Swanson,R.,
Sumner-Smith,M. and Soell,D.G.
TITLE Misaminoacylation by glutaminyl-tRNA synthetase: relaxed
specificity in wild-type and mutant enzymes
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 81, 2972-2976 (1984)
STANDARD full staff_review
COMMENT SWISS-PROT; P00962; SYQ$ECOLI.
From EMBL entry ECGLNS; dated 21-FEB-1991.
FEATURES Location/Qualifiers
repeat_unit 156..167
/note="inverted repeat"
repeat_unit 170..182
/note="inverted repeat"
promoter 266..272
/note="Pribnow box"
RBS 299..303
/note="Shine-Dalgarno sequence"
CDS 310..1962
/product="glutaminyl-tRNA synthetase"
/gene="glnS"
/codon_start=310
misc_signal 2170..2194
/note="pot. stem-loop structure transcription termination"
BASE COUNT 591 a 577 c 582 g 556 t
ORIGIN
1 ttagcccggt ggtttgcaga caatatcgaa cgatttcagt ggcttgatca ttaaagctca
61 gggccatatt gtggcgggtc aaacaccgct gtatgaaatc aaaaagtaat ctgctttatg
121 cctggcgtac gcttggcgtc gcatccaaca atgacaagcg gtggagatct tctctgccgc
181 tttttttttc atcaatcatc cccataatcc ttgttagatt atcaatttta aaaaactaac
241 agttgtcagc ctgtcccgct tataagatca tacgccgtta tacgttgttt acgctttgag
301 gaatccacga tgagtgaggc agaagcccgc ccgactaact ttatccgtca gatcatcgat
361 gaagatctgg ccagtggtaa gcacaccaca gtacacaccc gtttcccgcc ggagccgaat
421 ggctatctgc atattggcca tgcgaaatct atctgcctga acttcgggat cgcccaggac
481 tataaaggcc agtgcaacct gcgtttcgac gacactaacc cggtaaaaga agatatcgag
541 tatgttgagt cgatcaaaaa cgacgtagag tggttaggtt ttcactggtc tggtaacgtc
601 cgttactcct ccgattattt tgatcagctc cacgcctatg cgatcgaact gatcaataaa
661 ggcctggcgt acgttgatga actgacgccg gaacagatcc gcgaataccg cggcaccctg
721 acgcaaccgg gtaaaaacag cccgtaccgc gaccgcagcg ttgaagagaa cctggcgctg
781 ttcgaaaaaa tgcgtgccgg tggttttgaa gaaggtaaag cctgcctgcg tgcgaaaatc
841 gacatggctt caccgtttat cgtgatgcgc gatccggtgc tgtaccgtat taaatttgct
901 gaacaccacc agactggcaa caagtggtgc atctacccga tgtacgactt cacccactgc
961 atcagcgatg ccctggaagg tattacgcac tctctgtgta cgcttgagtt ccaggacaac
1021 cgtcgtctgt acgactgggt actggacaac atcacgattc ctgttcaccc gcgccagtat
1081 gagttctcgc gcctgaatct ggaatacacc gtgatgtcca agcgtaagtt gaacctgctg
1141 gtgaccgaca agcacgttga aggctgggat gacccgcgta tgccgaccat ttccggtctg
1201 cgtcgtcgtg gttacactgc ggcttctatt cgtgagttct gcaaacgcat cggcgtgacc
1261 aagcaggaca acaccattga gatggcgtcg ctggaatcct gcatccgtga agatctcaac
1321 gaaaatgcgc cgcgcgcaat ggcggttatc gatccggtga aactggttat cgaaaactat
1381 cagggcgaag gcgaaatggt taccatgccg aaccatccga acaaaccgga aatgggcagc
1441 cgtcaggtgc cgtttagcgg tgagatttgg attgatcgcg ccgatttccg cgaagaagct
1501 aacaagcagt acaaacgtct ggtgctgggt aaagaagtgc gtctgcgtaa tgcttatgtg
1561 attaaggcag aacgcgtcga gaaagatgcc gaaggtaata tcaccaccat cttctgtact
1621 tatgacgccg ataccttaag caaagatccg gcagatggtc gtaaagtcaa aggtgttatt
1681 cactgggtga gcgcggcaca tgcgctgccg gttgaaatcc gtttgtatga ccgtctgttc
1741 agcgtgccta acccaggtgc tgcggatgat ttcctgtcgg tgattaaccc ggaatcgctg
1801 gtgatcaaac agggctttgc tgaaccgtcg ctgaaagatg cggttgcggg taaagcattc
1861 cagtttgagc gtgaaggtta cttctgcctc gatagccgcc attctacggc ggaaaaaccg
1921 gtatttaacc gcaccgttgg gctgcgtgat actgggcgaa agtaggcgag taattttaag
1981 tttcgctatg ccggatgggg cgtttacgtc gcatccggca aggaacagac aaacagtttc
2041 aaacgctaaa ttgcctgatg cgctacgctt atcaggccta catgatctct gcaacacacc
2101 gagcccgcgt gcttttgcag gccggataag gcgttcgcgc cgcatccggc aagaaacagc
2161 aaacaatcca aaacgccgcg ttcggcggcg tttttctgct tttcttcgcg aattaattcc
2221 gcttcgcaat ttatccataa aataaattta aaataacaaa acataatcaa ataaaatgta
2281 aaccgctttc gcgttgcgga aaattt
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