GenBank-Updates@genbank.bio.net (05/18/91)
LOCUS ECOGDHB 1659 bp ds-DNA BCT 18-MAY-1991
DEFINITION E. coli gdhA gene for NADP-specific glutamate dehydrogenase
(EC1.4.1.4)
ACCESSION X00988 J01615 K00565
KEYWORDS dehydrogenase; inverted repeat.
SOURCE Escherichia coli DNA.
ORGANISM Escherichia coli
Prokaryota; Bacteria; Gracilicutes; Scotobacteria;
Facultatively anaerobic rods; Enterobacteriaceae.
REFERENCE 1 (bases 1 to 1659)
AUTHORS McPherson,M.J. and Wootton,J.C.
TITLE Complete nucleotide sequence of the Escherichia coli gdhA gene
JOURNAL Nucleic Acids Res. 11, 5257-5266 (1983)
STANDARD full automatic
COMMENT SWISS-PROT; P00370; DHE4$ECOLI.
From EMBL entry ECGDHA; dated 21-FEB-1991.
FEATURES Location/Qualifiers
promoter 8..57
/note="promoter region"
promoter 8..13
/note="-35 P3"
promoter 20..26
/note="-35 P2"
promoter 26..31
/note="-35 P1"
promoter 30..35
/note="-10 P3"
promoter 42..47
/note="-10 P2"
promoter 50..55
/note="-10 P1"
RBS 58..67
/note="put. Shine-Dalgarno sequence"
CDS 73..1413
/product="glutamate dehydrogenase"
/gene="gdhA"
/codon_start=73
repeat_unit 1426..1463
/note="inverted repeat"
repeat_unit 1482..1518
/note="inverted repeat"
repeat_unit 1493..1504
/note="inverted repeat"
repeat_unit 1508..1518
/note="inverted repeat"
misc_feature 1519..1525
/note="pot. transcription terminator"
BASE COUNT 411 a 412 c 443 g 393 t
ORIGIN
1 tcgaaaactg caaaagcaca tgacataaac aacataagca caatcgtatt aatatataag
61 ggttttatat ctatggatca gacatattct ctggagtcat tcctcaacca tgtccaaaag
121 cgcgacccga atcaaaccga gttcgcgcaa gccgttcgtg aagtaatgac cacactctgg
181 ccttttcttg aacaaaatcc aaaatatcgc cagatgtcat tactggagcg tctggttgaa
241 ccggagcgcg tgatccagtt tcgcgtggta tgggttgatg atcgcaacca gatacaggtc
301 aaccgtgcat ggcgtgtgca gttcagctct gccatcggcc cgtacaaagg cggtatgcgc
361 ttccatccgt cagttaacct ttccattctc aaattcctcg gctttgaaca aaccttcaaa
421 aatgccctga ctactctgcc gatgggcggt ggtaaaggcg gcagcgattt cgatccgaaa
481 ggaaaaagcg aaggtgaagt gatgcgtttt tgccaggcgc tgatgactga actgtatcgc
541 cacctgggcg cggataccga cgttccggca ggtgatatcg gggttggtgg tcgtgaagtc
601 ggctttatgg cggggatgat gaaaaagctc tccaacaata ccgcctgcgt cttcaccggt
661 aagggccttt catttggcgg cagtcttatt cgcccggaag ctaccggcta cggtctggtt
721 tatttcacag aagcaatgct aaaacgccac ggtatgggtt ttgaagggat gcgcgtttcc
781 gtttctggct ccggcaacgt cgcccagtac gctatcgaaa aagcgatgga atttggtgct
841 cgtgtgatca ctgcgtcaga ctccagcggc actgtagttg atgaaagcgg attcacgaaa
901 gagaaactgg cacgtcttat cgaaatcaaa gccagccgcg atggtcgagt ggcagattac
961 gccaaagaat ttggtctggt ctatctcgaa ggccaacagc cgtggtctct accggttgat
1021 atcgccctgc cttgcgccac ccagaatgaa ctggatgttg acgccgcgca tcagcttatc
1081 gctaatggcg ttaaagccgt cgccgaaggg gcaaatatgc cgaccaccat cgaagcgact
1141 gaactgttcc agcaggcagg cgtactattt gcaccgggta aagcggctaa tgctggtggc
1201 gtcgctacat cgggcctgga aatggcacaa aacgctgcgc gcctgggctg gaaagccgag
1261 aaagttgacg cacgtttgca tcacatcatg ctggatatcc accatgcctg tgttgagcat
1321 ggtggtgaag gtgagcaaac caactacgtg cagggcgcga acattgccgg ttttgtgaag
1381 gttgccgatg cgatgctggc gcagggtgtg atttaagttg taaatgcctg atggcgctac
1441 gcttatcagg cctacaaatg ggcacaattc attgcagtta cgctctaatg taggccgggc
1501 aagcgcagcg cccccggcaa aatttcaggc gtttatgagt atttaacgga tgatgctccc
1561 cacggaacat ttcttatggg ccaacggcat ttcttactgt agtgctccca aaactgcttg
1621 tcgtaacgat aacacgcttc aagttcagca tccgttaac
//