GenBank-Updates@genbank.bio.net (05/18/91)
LOCUS ECOMURGC 2793 bp ds-DNA BCT 18-MAY-1991
DEFINITION Escherichia coli murG and murC genes for an unidentified reading
frame and UDP-N-acetylmuramate:L-alanine ligase (EC 6.3.2.8)
respectively
ACCESSION X52644
KEYWORDS UDP-N-acetylmuramate:L-alanine ligase; ligase; murC gene;
murG gene; unidentified reading frame.
SOURCE Escherichia coli DNA.
ORGANISM Escherichia coli
Prokaryota; Bacteria; Gracilicutes; Scotobacteria;
Facultatively anaerobic rods; Enterobacteriaceae.
REFERENCE 1 (bases 1 to 2793)
AUTHORS Ikeda,M.
JOURNAL Unpublished (1990)
STANDARD full automatic
REFERENCE 2 (bases 1 to 2793)
AUTHORS Ikeda,M., Wachi,M., Jung,H.K., Ishino,F. and Matsuhashi,M.
TITLE Nucleotide sequence involving murG and murC in the mra gene cluster
region of Escherichia coli
JOURNAL Nucleic Acids Res. 18, 4014-4014 (1990)
STANDARD full automatic
COMMENT SWISS-PROT; P17443; MURG$ECOLI.
**map: map position=2mins;
From EMBL entry ECMURGC; dated 21-FEB-1991.
FEATURES Location/Qualifiers
CDS <1..82
/note="ftsW protein (partial) (27 AA) (1 is 3rd base in
codon)"
/codon_start=1
RBS 70..75
/note="put. ribosome binding site"
CDS 82..1146
/note="murG protein (AA 1-355)"
/codon_start=82
RBS 92..96
/note="put. ribosome binding site"
RBS 1182..1187
/note="put. ribosome binding site"
CDS 1203..2675
/note="murC protein (UDP-N-acetylmuramate: L-alanine
ligase) (AA 1-491)"
/codon_start=1203
RBS 2657..2661
/note="put. ribosome binding site"
CDS 2671..2793
/note="ddl protein (partial) (AA 1-41)"
/codon_start=2671
BASE COUNT 620 a 676 c 858 g 639 t
ORIGIN
1 gtcgacagcc atcatgatgc tgttgcgtat tgattatgaa acgcgtctgg agaaagcgca
61 ggcgtttgta cgaggttcac gatgagtggt caaggaaagc gattaatggt gatggcaggc
121 ggaaccggtg gacatgtatt cccgggactg gcggttgcgc accatctaat ggctcagggt
181 tggcaagttc gctggctggg gactgccgac cgtatggaag cggacttagt gccaaaacat
241 ggcatcgaaa ttgatttcat tcgtatctct ggtctgcgtg gaaaaggtat aaaagcactg
301 atagctgccc cgctgcgtat cttcaacgcc tggcgtcagg cgcgggcgat tatgaaagcg
361 tacaaacctg acgtggtgct cggtatggga ggctacgtgt caggtccagg tggtctggcc
421 gcgtggtcgt taggcattcc ggttgtactt catgaacaaa acggtattgc gggcttaacc
481 aataaatggc tggcgaagat tgccaccaaa gtgatgcagg cgtttccagg tgctttccct
541 aatgcggaag tagtgggtaa cccggtgcgt accgatgtgt tggcgctgcc gttgccgcag
601 caacgtttgg ctggacgtga aggtccggtt cgtgtgctgg tagtgggtgg ttctcagggc
661 gcacgcattc ttaaccagac aatgccgcag gttgctgcga aactgggtga ttcagtcact
721 atctggcatc agagcggcaa aggttcgcaa caatccgttg aacaggcgta tgccgaagcg
781 gggcaaccgc agcataaagt gacggaattt attgatgata tggcggcggc gtatgcgtgg
841 gcggatgtcg tcgtttgccg ctccggtgcg ttaacggtga gtgaaatcgc cgcggcagga
901 ctaccggcgt tgtttgtgcc gtttcaacat aaagaccgcc agcaatactg gaatgcgcta
961 ccgctggaaa aagcgggcgc agccaaaatt atcgagcagc cacagcttag cgtggatgct
1021 gtcgccaaca ccctggccgg gtggtcgcga gaaaccttat taaccatggc agaacgcgcc
1081 cgcgctgcat ccattccgga tgccaccgag cgagtggcaa atgaagtgag ccgggttgcc
1141 cgggcgtaat tgtagcgatg ccttttgcat cgtatgaatt taagaagtta atggcgtaaa
1201 gaatgaatac acaacaattg gcaaaactgc gttccatcgt gcccgaaatg cgtcgcgttc
1261 ggcacataca ttttgtcggc attggtggtg ccggtatggg cggtattgcc gaagttctgg
1321 ccaatgaagg ttatcagatc agtggttccg atttagcgcc aaatccggtc acgcagcagt
1381 taatgaatct gggtgcgacg atttatttca accatcgccc ggaaaacgta cgtgatgcca
1441 gcgtggtcgt tgtttccagc gcgatttctg ccgataaccc ggaaattgtc gccgctcatg
1501 aagcgcgtat tccggtgatc cgtcgtgccg aaatgctggc tgagttaatg cgttttcgtc
1561 atggcatcgc cattgccgga acgcacggca aaacgacaac caccgcgatg gtttccagca
1621 tctacgcaga agcggggctc gacccaacct tcgttaacgg cgggctggta aaagcggcgg
1681 gggttcatgc gcgtttgggg catggtcggt acctgattgc cgaagcagat gagagtgatg
1741 catcgttcct gcatctgcaa ccgatggtgg cgattgtcac caatatcgaa gccgaccaca
1801 tggataccta ccagggcgac tttgagaatt taaaacagac ttttattaat tttctgcaca
1861 acctgccgtt ttacggtcgt gcggtgatgt gtgttgatga tccggtgatc cgcgaattgt
1921 taccgcgagt ggggcgtcag accacgactt acggcttcag cgaagatgcc gacgtgcgtg
1981 tagaagatta tcagcagatt ggcccgcagg ggcactttac gctgctgcgc caggacaaag
2041 agccgatgcg cgtcaccctg aatgcgccag gtcgtcataa cgcgctgaac gccgcagctg
2101 cggttgcggt tgctacggaa gagggcattg acgacgaggc tattttgcgg gcgcttgaaa
2161 gcttccaggg gactggtcgc cgttttgatt tcctcggtga attcccgctg gagccagtga
2221 atggtaaaag cggtacggca atgctggtcg atgactacgg ccaccacccg acggaagtgg
2281 acgccaccat taaagcggcg cgcgcaggct ggccggataa aaacctggta atgctgtttc
2341 agccgcaccg ttttacccgt acgcgcgacc tgtatgatga tttcgccaat gtgctgacgc
2401 aggttgatac cctgttgatg ctggaagtgt atccggctgg cgaagcgcca attccgggag
2461 cggacagccg ttcgctgtgt cgcacaattc gtggacgtgg gaaaattgat cccattctgg
2521 tgccggatcc ggcgcgggta gccgagatgc tggcaccggt attaaccggt aacgacctga
2581 ttctcgttca gggggctggt aatattggaa aaattgcccg ttctttagct gaaatcaaac
2641 tgaagccgca aactccggag gaagaacaac atgactgata aaatcgcggt cctgttgggt
2701 gggacctccg ctgagcggga agtttctctg aattctggcg cagcggtgtt agccggactg
2761 cgtgaaggcg gtattgacgc gtatcctgtc gac
//