GenBank-Updates@genbank.bio.net (05/18/91)
LOCUS ECOGLYWG 890 bp ds-DNA BCT 18-MAY-1991
DEFINITION E. coli glyW region, a duplicated gene for tRNA-Gly-3
ACCESSION X03239 M14391
KEYWORDS insertion sequence; inverted repeat; transfer rna-gly;
unidentified reading frame.
SOURCE Escherichia coli DNA.
ORGANISM Escherichia coli
Prokaryota; Bacteria; Gracilicutes; Scotobacteria;
Facultatively anaerobic rods; Enterobacteriaceae.
REFERENCE 1 (bases 110 to 185)
AUTHORS Tucker,S.D., Gopalakrishnan,A.S., Bollinger,R., Dowhan,W. and
Murgola,E.J.
TITLE Molecular mapping of gly W, a duplicate gene for gly-tRNA-3 of
Escherichia coli
JOURNAL J. Bacteriol. 152, 773-779 (1982)
STANDARD full automatic
REFERENCE 2 (bases 1 to 890)
AUTHORS Tucker,S.D. and Murgola,E.J.
TITLE Sequence analysis of the glyW region in Escherichia coli.
JOURNAL Biochimie 67, 1053-1057 (1985)
STANDARD full automatic
COMMENT SWISS-PROT; P06979; YGYW$ECOLI.
Data kindly reviewed (27-MAR-1986) by S. Tucker
From EMBL entry ECGLYWG; dated 22-FEB-1991.
FEATURES Location/Qualifiers
insertion_seq <1..10
/note="IS1 insertion sequence"
promoter 11..14
/note="pot. -35 sequence"
promoter 31..37
/note="pot. -10 sequence"
repeat_unit 53..57
/note="imp. inverted repeat A"
repeat_unit 63..68
/note="imp. inverted repeat A'"
tRNA 110..185
/note="mature transfer RNA-Gly-3"
misc_signal 215..232
/note="pot. transcription termination loop structure"
repeat_unit 215..220
/note="imp. inverted repeat B"
repeat_unit 227..232
/note="imp. inverted repeat B'"
repeat_unit 456..463
/note="inverted repeat C"
repeat_unit 468..475
/note="inverted repeat C'"
promoter 491..496
/note="pot. -35 sequence"
promoter 514..520
/note="pot. -10 sequence"
CDS 608..835
/note="unidentified reading frame"
/codon_start=608
BASE COUNT 230 a 202 c 248 g 210 t
ORIGIN
1 agtcattacc gactcatcgc gccaggtaag tagaatgcaa cgcatcgaac ggcggcactg
61 attgccagac gataataaaa tcaagtgatt aactgattgc ttgatgaatg cgggaatagc
121 tcagttggta gagcacgacc ttgccaaggt cggggtcgcg agttcgagtc tcgtttcccg
181 ctccagttta aaagacatcg gcgtcaagcg gatgtctggc tgaaaggcct gaagaatttg
241 gcgcgttaac aaagcggtta tgtagcggat tgcaaatccg tctagtccgg ttcgactccg
301 gaacgcgcct ccactttctt cccgagcccg gatggtggaa tcggtagaca caagggattt
361 aaaatccctc ggcgttcgcg ctgtgcggtt caagttcccg ctccgggtac catgggaaag
421 ataagaataa aatcaaagca ataagcagtg tcgtgaaacc accttcgggt ggtttttttg
481 tgcctgcaac ttgtcgttac accctcctta atttttaatc accagcaaag ccactcaaaa
541 agtatctctg atacggaccg gcatgtaaga taggtgctgg cgagttgaga tccacaagga
601 aaagcgtatg aaaacgggac cgttaaacga aagtgagttg gaatggctgg acgatattct
661 gaccaaatac aacactgacc acgccatcct tgatgtggcg gagctggacg gtttattgac
721 ggcggtgttg agttctccgc aagagattga accggaacag tggctggttg ccgtgtgggg
781 tggggctgac tatgtgccgc gctgggcgtc agagaaagag atgacgcgcg ctttatgaat
841 ctggcttttc aacatatggc cgataccgca gagcgtctga acgaattctt
//