GenBank-Updates@genbank.bio.net (03/07/91)
LOCUS ECOTYRR 1965 bp ds-DNA BCT 07-MAR-1991
DEFINITION E.coli K12 tyrR regulatory gene encoding TyrR protein, complete
cds.
ACCESSION M12114
KEYWORDS tyrR gene.
SOURCE E.coli K12, clone pMU360.
ORGANISM Escherichia coli
Prokaryota; Bacteria; Gracilicutes; Scotobacteria; Facultatively
anaerobic rods; Enterobacteriaceae.
REFERENCE 1 (bases 1 to 1965)
AUTHORS Cornish,E.C., Argyropoulos,V.P., Pittard,J. and Davidson,B.E.
TITLE Structure of the Escherichia coli K12 regulatory gene tyrR:
Nucleotide sequence and sites of initiation of transcription and
translation
JOURNAL J. Biol. Chem. 261, 403-410 (1986)
STANDARD full staff_review
REFERENCE 2 (bases 1 to 1965)
AUTHORS Ganesan,S.
JOURNAL Unpublished (1991)
STANDARD full staff_review
COMMENT Draft entry and computer readable copy of sequence kindly
provided by B.E.Davidson, 06-SEP-1985.
The regulatory tyrR gene is autogenously regulated. A stem-loop
structure, which could be a rho-independent mRNA terminator, is
located at positions 1865-1899.
FEATURES Location/Qualifiers
mRNA 290..1904
/note="tyrR mRNA"
CDS 318..1859
/note="TyrR protein"
/codon_start=318
BASE COUNT 468 a 463 c 545 g 489 t
ORIGIN 189 bp upstream of HpaII site; 28.8 min on K12 map
1 gggattggtg caggacttct gacggcacga ctcgggatta aagctatgga gctttgccgc
61 ccgctgccgt ggattgacga tgacaaacct cgcctcgggg atttccgtcg tcagcttatc
121 ggtcaggtga aagaaacgct gcaaaaaggc aaaagcccag cgaaaaataa tgcaatatcg
181 ggtgctgacc ggatatcttt acgccgaagt gcccgttttt ccgtctttgt gtcaatgatt
241 gttgacagaa accttcctgc tatccaaata gtgtcatatc atcatattaa ttgttctttt
301 ttcaggtgaa ggttcccatg cgtctggaag tcttttgtga agaccgactc ggtctgaccc
361 gcgaattact cgatctactc gtgctaagag gcattgattt acgcggtatt gagattgatc
421 ccattgggcg aatctacctc aattttgctg aactggagtt tgagagtttc agcagtctga
481 tggccgaaat acgccgtatt gcgggtgtta ccgatgtgcg tactgtcccg tggatgcctt
541 ccgaacgtga gcatctggcg ttgagcgcgt tactggaggc gttgcctgaa cctgtgctct
601 ctgtcgatat gaaaagcaaa gtggatatgg cgaacccggc gagctgtcag ctttttgggc
661 aaaaattgga tcgcctgcgc aaccataccg ccgcacaatt gattaacggc tttaattttt
721 tacgttggct ggaaagcgaa ccgcaagatt cgcataacga gcatgtcgtt attaatgggc
781 agaatttcct gatggagatt acgcctgttt atcttcagga tgaaaatgat caacacgtcc
841 tgaccggtgc ggtggtgatg ttgcgatcaa cgattcgtat gggccgccag ttgcaaaatg
901 tcgccgccca ggacgtcagc gccttcagtc aaattgtcgc cgtcagcccg aaaatgaagc
961 atgttgtcga acaggcgcag aaactggcga tgctaagcgc gccgctgctg attacgggtg
1021 acacaggtac aggtaaagat ctctttgcct acgcctgcca tcaggcaagc cccagagcgg
1081 gcaaacctta cctggcgctg aactgtgcgt ctataccgga agatgcggtc gagagtgaac
1141 tgtttggtca tgctccggaa gggaagaaag gattctttga gcaggcgaac ggtggttcgg
1201 tgctgttgga tgaaataggg gaaatgtcac cacggatgca ggcgaaatta ctgcgtttcc
1261 ttaatgatgg cactttccgt cgggttggcg aagaccatga ggtgcatgtc gatgtgcggg
1321 tgatttgcgc tacgcagaag aatctggtcg aactggtgca aaaaggcatg ttccgtgaag
1381 atctctatta tcgtctgaac gtgttgacgc tcaatctgcc gccgctacgt gactgtccgc
1441 aggacatcat gccgttaact gagctgttcg tcgcccgctt tgccgacgag cagggcgtgc
1501 cgcgtccgaa actggccgct gacctgaata ctgtacttac gcgttatgcg tggccgggaa
1561 atgtgcggca gttaaagaac gctatctatc gcgcactgac acaactggac ggttatgagc
1621 tgcgtccaca ggatattttg ttgccggatt atgacgccgc aacggtagcc gtgggcgaag
1681 atgcgatgga aggttcgctg gacgaaatca ccagccgttt tgaacgctcg gtattaaccc
1741 agctttatcg caattatccc agcacgcgca aactggcaaa acgtctcggc gtttcacata
1801 ccgcgattgc caataagttg cgggaatatg gtctgagtca gaagaagaac gaagagtaag
1861 cgcgaatatg cctgatggtg caacaccatc aggcatatta aattatgctt tcagtacagc
1921 cagagctgct tcgtaatccg gctcggtggt gatttcatcc accag
//GenBank-Updates@genbank.bio.net (05/18/91)
LOCUS ECOTYRR 1965 bp ds-DNA BCT 18-MAY-1991
DEFINITION E.coli K12 tyrR regulatory gene encoding TyrR protein, complete
cds.
ACCESSION M12114
KEYWORDS tyrR gene.
SOURCE E.coli K12, clone pMU360.
ORGANISM Escherichia coli
Prokaryota; Bacteria; Gracilicutes; Scotobacteria;
Facultatively anaerobic rods; Enterobacteriaceae.
REFERENCE 1 (bases 1 to 1965)
AUTHORS Cornish,E.C., Argyropoulos,V.P., Pittard,J. and Davidson,B.E.
TITLE Structure of the Escherichia coli K12 regulatory gene tyrR:
Nucleotide sequence and sites of initiation of transcription and
translation
JOURNAL J. Biol. Chem. 261, 403-410 (1986)
STANDARD full staff_review
REFERENCE 2 (bases 1 to 1965)
AUTHORS Ganesan,S.
JOURNAL Thesis (1989) , University of Melbourne
STANDARD full staff_review
COMMENT Draft entry and computer readable copy of sequence kindly
provided by B.E.Davidson, 06-SEP-1985.
The regulatory tyrR gene is autogenously regulated. A stem-loop
structure, which could be a rho-independent mRNA terminator, is
located at positions 1865-1899.
FEATURES Location/Qualifiers
mRNA 290..1904
/note="tyrR mRNA"
CDS 318..1859
/note="TyrR protein"
/codon_start=318
BASE COUNT 468 a 463 c 545 g 489 t
ORIGIN 189 bp upstream of HpaII site; 28.8 min on K12 map
1 gggattggtg caggacttct gacggcacga ctcgggatta aagctatgga gctttgccgc
61 ccgctgccgt ggattgacga tgacaaacct cgcctcgggg atttccgtcg tcagcttatc
121 ggtcaggtga aagaaacgct gcaaaaaggc aaaagcccag cgaaaaataa tgcaatatcg
181 ggtgctgacc ggatatcttt acgccgaagt gcccgttttt ccgtctttgt gtcaatgatt
241 gttgacagaa accttcctgc tatccaaata gtgtcatatc atcatattaa ttgttctttt
301 ttcaggtgaa ggttcccatg cgtctggaag tcttttgtga agaccgactc ggtctgaccc
361 gcgaattact cgatctactc gtgctaagag gcattgattt acgcggtatt gagattgatc
421 ccattgggcg aatctacctc aattttgctg aactggagtt tgagagtttc agcagtctga
481 tggccgaaat acgccgtatt gcgggtgtta ccgatgtgcg tactgtcccg tggatgcctt
541 ccgaacgtga gcatctggcg ttgagcgcgt tactggaggc gttgcctgaa cctgtgctct
601 ctgtcgatat gaaaagcaaa gtggatatgg cgaacccggc gagctgtcag ctttttgggc
661 aaaaattgga tcgcctgcgc aaccataccg ccgcacaatt gattaacggc tttaattttt
721 tacgttggct ggaaagcgaa ccgcaagatt cgcataacga gcatgtcgtt attaatgggc
781 agaatttcct gatggagatt acgcctgttt atcttcagga tgaaaatgat caacacgtcc
841 tgaccggtgc ggtggtgatg ttgcgatcaa cgattcgtat gggccgccag ttgcaaaatg
901 tcgccgccca ggacgtcagc gccttcagtc aaattgtcgc cgtcagcccg aaaatgaagc
961 atgttgtcga acaggcgcag aaactggcga tgctaagcgc gccgctgctg attacgggtg
1021 acacaggtac aggtaaagat ctctttgcct acgcctgcca tcaggcaagc cccagagcgg
1081 gcaaacctta cctggcgctg aactgtgcgt ctataccgga agatgcggtc gagagtgaac
1141 tgtttggtca tgctccggaa gggaagaaag gattctttga gcaggcgaac ggtggttcgg
1201 tgctgttgga tgaaataggg gaaatgtcac cacggatgca ggcgaaatta ctgcgtttcc
1261 ttaatgatgg cactttccgt cgggttggcg aagaccatga ggtgcatgtc gatgtgcggg
1321 tgatttgcgc tacgcagaag aatctggtcg aactggtgca aaaaggcatg ttccgtgaag
1381 atctctatta tcgtctgaac gtgttgacgc tcaatctgcc gccgctacgt gactgtccgc
1441 aggacatcat gccgttaact gagctgttcg tcgcccgctt tgccgacgag cagggcgtgc
1501 cgcgtccgaa actggccgct gacctgaata ctgtacttac gcgttatgcg tggccgggaa
1561 atgtgcggca gttaaagaac gctatctatc gcgcactgac acaactggac ggttatgagc
1621 tgcgtccaca ggatattttg ttgccggatt atgacgccgc aacggtagcc gtgggcgaag
1681 atgcgatgga aggttcgctg gacgaaatca ccagccgttt tgaacgctcg gtattaaccc
1741 agctttatcg caattatccc agcacgcgca aactggcaaa acgtctcggc gtttcacata
1801 ccgcgattgc caataagttg cgggaatatg gtctgagtca gaagaagaac gaagagtaag
1861 cgcgaatatg cctgatggtg caacaccatc aggcatatta aattatgctt tcagtacagc
1921 cagagctgct tcgtaatccg gctcggtggt gatttcatcc accag
//