GenBank-Updates@genbank.bio.net (05/18/91)
LOCUS PT4RIIA 2834 bp ds-DNA PHG 18-MAY-1991
DEFINITION Bacteriophage T4 rIIA gene and ORF rIIA.1.
ACCESSION X52686 X00905
KEYWORDS membrane protein; rIIA gene.
SOURCE Bacteriophage T4 DNA.
ORGANISM Bacteriophage T4
Viridae; ds-DNA nonenveloped viruses; Myoviridae.
REFERENCE 1 (bases 2736 to 2834)
AUTHORS Pribnow,D., Sigurdson,D.C., Gold,L., Singer,B.S. and Napoli,C.
TITLE rII cistrons of bacteriophage T4
JOURNAL J. Mol. Biol. 149, 337-376 (1981)
STANDARD full automatic
REFERENCE 2 (bases 1971 to 2735)
AUTHORS Sugino,A. and Drake,J.W.
TITLE Modulation of mutation in bacteriophage T4 by a base-pair change a
dozen nucleotides removed
JOURNAL J. Mol. Biol. 176, 239-249 (1984)
STANDARD full automatic
REFERENCE 3 (bases 1 to 2834)
AUTHORS Brody,E.N.
JOURNAL Unpublished (1990)
STANDARD full automatic
REFERENCE 4 (bases 1 to 2834)
AUTHORS Daegelen,P. and Brody,E.
TITLE The rIIA gene of bacteriophage T4. I. Its DNA sequence and
discovery of a new open reading frame between genes 60 and rIIA
JOURNAL Genetics 125, 237-248 (1990)
STANDARD full automatic
COMMENT *source: strain=isolate pTB101; See also <M19728>;
From EMBL entry ECRIIA; dated 28-JAN-1991.
FEATURES Location/Qualifiers
CDS <1..275
/note="for complete sequence see M19728"
/gene="gene 60"
/evidence=EXPERIMENTAL
/codon_start=1
prim_transcript 313..>2724
/note="+1 of P1 early promoter"
/evidence=EXPERIMENTAL
CDS 333..536
/note="ORF rIIA.1"
/codon_start=333
prim_transcript 405..>2724
/note="+1 of P2 early promoter"
prim_transcript 473..>2724
/note="+1 of Pm middle promoter"
/evidence=EXPERIMENTAL
prim_transcript 474..>2724
/note="+1 of Pm middle promoter"
/evidence=EXPERIMENTAL
CDS 547..2724
/gene="rIIA"
/evidence=EXPERIMENTAL
/codon_start=547
conflict 2392..2392
/note="replace (2392,'')"
/citation=[2]
conflict 2503..2503
/note="replace(2350,'')"
/citation=[2]
BASE COUNT 928 a 427 c 557 g 922 t
ORIGIN
1 gaattcgctt tgtcaaaact cctgtaatca tcgctcaggt cggtaaaaaa caagaatggt
61 tttatacagt cgctgaatat gagagtgcca aagatgctct acctaaacat agcatccgtt
121 atattaaggg acttggctct ttggaaaaat ctgaatatcg tgagatgatt caaaacccag
181 tatatgatgt tgttaaactt cctgagaact ggaaagagct ttttgaaatg ctcatgggag
241 ataatgctga ccttcgtaaa gaatggatga gccagtagtt tactttacca caaggatgtg
301 gtataattaa ttgggcaaat gaggatattg aaatgaaatc atataaagta aatttagaac
361 tttttgataa agcagttcat cgagaatata gaatcattca acgctttttc gatatgggag
421 aagccgaaga atttaaaacc cgctttaaag atattagaga taaaattcaa tccgacaccg
481 caactaaaga tgaactacta gaagttgctg aagttattaa gcgtaatatg aattaatgag
541 gaaattatga ttatcaccac tgaaaaagaa acaattcttg gtaatggttc taaatcaaaa
601 gcatttagca tcacagcatc tcctaaagta tttaaaattc tgtcatctga tttgtataca
661 aacaagattc gcgcagtagt ccgtgaattg attactaaca tgattgatgc tcatgcactt
721 aatggaaatc ctgaaaaatt tatcatacaa gttcctggac gtttagaccc acgatttgtt
781 tgtcgagatt ttggtccggg tatgagtgat tttgatattc aaggtgatga taattctcct
841 gggttgtata attcatactt cagttcatct aaagctgaat ctaatgactt tattggcgga
901 tttggtttag gttctaaatc tccgtttagt tatactgata cgtttagtat tacttcgtat
961 cataaaggtg aaattcgtgg ttatgtagct tacatggatg gtgatggtcc acagattaaa
1021 cctacattcg taaaagaaat gggtccagat gataaaactg gtattgaaat cgtagttcca
1081 gttgaagaaa aagactttag aaactttgct tatgaagttt cttatatcat gcgaccgttc
1141 aaagatttgg ctatcattaa tggtcttgac cgcgaaattg attattttcc ggattttgat
1201 gactattacg gtgtaaatcc agaaagatac tggcctgatc gtggtggatt atatgctatc
1261 tacggtggta ttgtttatcc tatcgatggt gttattagag accgtaactg gctaagcatt
1321 cgcaatgaag tgaattacat taagtttcca atgggttcac ttgatattgc tccatctcgc
1381 gaggctcttt cactggatga tcgcactcgt aaaaatatta ttgaacgagt taaagaactc
1441 agtgagaaag catttaatga agatgtaaaa cgatttaaag aatctacatc tcctcgtcac
1501 acatatcgtg aattgatgaa gatggggtat tctgctcgag attatatgat tagtaattca
1561 gtcaaattca cgactaaaaa tctgtcatat aaaaagatgc agagcatgtt tgaacctgac
1621 agtaagttat gcaacgcggg agttgtgtat gaagtaaatc ttgaccctcg actgaagcgc
1681 attaagcaaa gtcatgaaac ttcagccgtt gcatcaagtt atcgtctgtt tggtattaat
1741 acaacaaaaa ttaatatcgt tattgataat attaaaaatc gtgttaatat tgtccgtgga
1801 ttagcacgtg cgttagatga tagtgaattt aataacactt tgaatattca tcataacgaa
1861 cgtcttctgt ttattaatcc agaagtagaa tcgcagattg atttgcttcc tgatattatg
1921 gcgatgtttg aaagtgatga agttaacatt cattatttgt cagaaattga agctttagta
1981 aaaagttata ttccaaaggt agttaaaagt aaagctcctc gtcctaaagc tgctacagcg
2041 tttaagtttg aaattaaaga cgggcgctgg gaaaagagga attatttacg cctcacatca
2101 gaagcagatg aaattactgg ttatgtagcg tatatgcatc gttctgatat tttctctatg
2161 gatggtacta catctctttg tcatccatct atgaatattt tgattcgtat ggctaatctt
2221 attggcatta atgaatttta tgttattcgt ccgcttttac agaaaaaggt aaaagaactc
2281 ggtcagtgcc aatgtatttt tgaagctttg cgtgatttat atgtagatgc ttttgatgat
2341 gtagattatg ataagtatgt aggttattca agttcagcta aacgatatat tgataaaatt
2401 atcaagtatc ctgagttaga ttttatgatg aagtacttca gtatagatga agtttctgaa
2461 gaatatacac gactcgctaa tatggttagt tcattacagg gtgtatattt taatggtgga
2521 aaagatacca tcggtcatga catttggaca gtaactaatc tttttgatgt attatcaaat
2581 aatgcttcaa aaaacagtga taaaatggtt gctgagttta ccaagaaatt ccgtattgtt
2641 tccgacttca tcggttatcg caactcttta agtgatgatg aagtttccca aatcgctaaa
2701 actatgaagg cccttgcggc ctaataagga aaattatgta caatattaaa tgcctgacca
2761 aaaacgaaca agctgaaatt gttaaactgt attcaagtgg taattacacc caacaggaat
2821 tggctgattg gcaa
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