GenBank-Updates@genbank.bio.net (05/18/91)
LOCUS DDISP61 2098 bp ds-DNA INV 18-MAY-1991
DEFINITION Dictyostelium discoideum SP60 gene for spore coat protein
ACCESSION X52105
KEYWORDS SP60 gene; spore coat protein.
SOURCE Dictyostelium discoideum DNA.
ORGANISM Dictyostelium discoideum
Eukaryota; Animalia; Protozoa; Sarcomastigophora; Sarcodina;
Rhizopoda; Eumycetozoa; Dictyostelia; Dictyosteliida;
Dictyosteliidae.
REFERENCE 1 (bases 1 to 2098)
AUTHORS Firtel,R.A.
JOURNAL Unpublished (1990)
STANDARD full automatic
REFERENCE 2 (bases 1 to 2098)
AUTHORS Haberstroh,L. and Firtel,R.A.
TITLE A spatial gradient of expression of a cAMP-regulated prespore
cell-type-specific gene Dictyostelium
JOURNAL Genes Dev. 4, 596-612 (1990)
STANDARD full automatic
COMMENT SWISS-PROT; P15270; SP60$DICDI.
From EMBL 26 entry DDSP60; dated 30-MAY-1990.
FEATURES Location/Qualifiers
precursor_RNA 1324..>1323
/note="primary transcript"
mRNA 1324..1575
/note="exon 1"
CDS 1422..1575
/note="spore coat protein (AA 1-51) (1575 is 1st base in
codon)"
/codon_start=1422
intron 1576..1695
/note="intron I"
mRNA 1696..>2098
/note="exon 2"
CDS 1696..>2098
/note="spore coat protein (AA 52-187) (1696 is 2nd base in
codon) (2098 is 2nd base in codon)"
/codon_start=1696
BASE COUNT 954 a 208 c 201 g 735 t
ORIGIN
1 agatctcccc ttaaagaata tcaaaataaa aagagtttat aaatgaaata ataaaaaaaa
61 taaaaaaaac taaaaaagaa aaaaaaaaca aaataaaatt aaataaaaaa acaaaaacaa
121 aacaaaagaa ataaataaaa ataaatacct ttattttttt tatatttatt tttattttgg
181 aaaaaataaa ataaactttt ttttatacaa tcactaatat atttttttaa taaattcaaa
241 tggaaattta tttttgaact tcaatatatt aagaattatt tcaaattaat taatcaaatg
301 aagaaaaaac atataataga atttttatta atttttattt ctttagatgg ttataaaaaa
361 acataatctt ttgaagggaa aaaaagttaa atttactcta agataactaa tactcaaact
421 catttttaaa ttaaggaaaa tcaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaatgtaatt
481 ttgttttttt attttatttt ttaacctatt taattaaaaa agattttact gggaatatcc
541 aaatttaaaa aatcattgtg attaaaatca taggtatgat aatctacaaa aaatatacat
601 ttttattaaa aaaaaataaa aaaataaaaa aaaaaaagaa ataaaaaaaa taaaaattag
661 cccaaaaaaa aaatcccata ctacattaaa atatttgtat atcaatcaga aatttaaatt
721 aaattgtttt ttttttcaca cacccacaca ctaatttacc ccatttttta catttctcat
781 ataaaaatta tttattgtac attgttcaat attcattcac acattaacac actttcaact
841 caattatttt tttttccaaa aaaacaataa tatatatata cactgtgaga attttctatt
901 aatagcgata gaaaaaaatt ttattttcaa acacactccc aacacacaag catatgaaaa
961 aactcacacc aattataatt tgtaaaacat agaacaaatt taataattat tttttattaa
1021 attgtattat ttttttttat tatttattta ttttttattt tcaaaaaaaa aaaaaaaaag
1081 ttaacagaca aaaagtataa tctatttaat tattcaaaaa aaaaaatatt aaatcattgt
1141 agtattttgt tcatattcaa tttaaatatc aatacaatta aaaaaagtta aaatgtaatt
1201 attaatatat atatatattt atcaatatat aatttaatat aaaaaaaaaa aagaaattta
1261 ataaacatat aatatttata taaaatcata aatttaaaat aaataatttt tttaatattt
1321 aaaaattcta ataaaaagtt ttttatattt agtaaatttg taaaatcaat ttgtaacaaa
1381 aactagtaat ttaaaaaaaa aaaaaacaaa aaaaaaaaaa aatgaagatt ttatcattat
1441 tagttgtagg tgccttatgt atgggaggta aagtttatgg tgaagttaat ggtgattgga
1501 acaataatgg tgattggaac aataatggcg attggaacaa taatggtgat tggaacaata
1561 atggtccaat tttaggtaag ttttatttta aactatatac ataaaaataa taaaataaaa
1621 aaatttggta ttaatacttt tttttttttt tttttttttt atttattatt attaaatctt
1681 gactttttta tatagactgt tcaacacttc aatgtccacc aagatatcat tgtgaagtaa
1741 ataatggtaa tagacaatgt gttgaagatc aaattacatt gccaccattt gataaatgtg
1801 ataatgtcca ttgtccaaaa ggatttaatt gcaaatatga ttgggaaaaa gatcttgctc
1861 tttgtgttcc atggagacca tatccaccag tttgtagaac tagatgtcca gaaggtcatg
1921 aatgtaaagt tgatgaatgg ggtaaagaat gttgcgtaaa gatcaaatgt gatgatattt
1981 gtgacttgcg ttgtccaaag ggtcatgaat gcaagatcaa acatgatggt agtaaatgct
2041 gtgtccgttc atggagacca agaccacata aaccacatcc acgtccacca atctgcag
//GenBank-Updates@genbank.bio.net (05/18/91)
LOCUS DDISP60G 2370 bp ds-DNA INV 18-MAY-1991
DEFINITION Dictyostelium discoideum SP60 gene for spore coat protein
ACCESSION X51892
KEYWORDS SP60 gene; spore coat protein.
SOURCE Dictyostelium discoideum DNA.
ORGANISM Dictyostelium discoideum
Eukaryota; Animalia; Protozoa; Sarcomastigophora; Sarcodina;
Rhizopoda; Eumycetozoa; Dictyostelia; Dictyosteliida;
Dictyosteliidae.
REFERENCE 1 (bases 1 to 2370)
AUTHORS Hames,D.
JOURNAL Unpublished (1990)
STANDARD full automatic
REFERENCE 2 (bases 1 to 2370)
AUTHORS Widdowson,D.C., Proffitt,J.A., Jagger,P.S., Richards,A.J. and
Hames,B.D.
TITLE Developmental expression and characterization of the gene encoding
spore coat protein SP60 in Dictyostelium discoideum.
JOURNAL Mol. Microbiol. 4, 951-960 (1990)
STANDARD full automatic
COMMENT *source: developmental stage=culmination; strain=AX2; X51892 is
correcting D.d. SP60 seq reported previously, see <M26239>.
From EMBL 26 entry DDSP60G; dated 12-SEP-1990.
FEATURES Location/Qualifiers
misc_feature 4..18
/note="C-rich (pot. regulatory) element"
misc_feature 25..30
/note="C-rich (pot. regulatory) element"
misc_feature 85..89
/note="C-rich (pot. regulatory) element"
misc_feature 95..99
/note="C-rich (pot. regulatory) element"
misc_feature 148..156
/note="C-rich (pot. regulatory) element"
misc_feature 199..207
/note="C-rich (pot. regulatory) element"
misc_feature 210..218
/note="C-rich (pot. regulatory) element"
misc_feature 230..237
/note="C-rich (pot. regulatory) element"
promoter 508..522
/note="TATA box/oligodA promoter motif"
precursor_RNA 557..>2079
/note="SP60 transcript"
mRNA 557..840
/note="exon 1"
CDS 687..840
/note="spore coat protein SP60 precursor (840 is 1st base
in codon)"
/codon_start=687
CDS 687..755
/note="signal peptide"
/codon_start=687
CDS 756..840
/note="mat. spore coat protein SP60 (840 is 1st base in
codon)"
/codon_start=756
intron 841..959
/note="intron I"
mRNA 960..>2079
/note="exon 2"
CDS 960..2077
/note="spore coat protein SP60 (AA 52-424) (960 is 2nd
base in codon)"
/codon_start=960
misc_feature 2227..2232
/note="pot. polyadenylation signal"
CDS complement(2369..2370)
/note="ORF (2369 is second base in codon)"
/codon_start=2370
BASE COUNT 955 a 253 c 348 g 814 t
ORIGIN
1 tttcacacac ccacacacta atttacccca ttttttacat ttctcatata aaaattattt
61 attgtacatt gttcaatatt cattcacaca ttaacacact ttcaactcaa ttattttttt
121 ttccaaaaaa acaataatat atatatacac tgtgagaatt ttctattaat agcgatagaa
181 aaaaatttta ttttcaaaca cactcccaac acacaagcat atgaaaaaac tcacaccaat
241 tataatttgt aaaacataga acaaatttaa taattatttt ttattaaatt gtattatttt
301 tttttattat ttatttattt tttattttca aaaaaaaaaa aaaaaagtta acagacaaaa
361 agtataatct atttaattat tcaaaaaaaa aaatattaaa tcattgtagt attttgttca
421 tattcaaatt aaatatcaat acaattaaaa aaagttaaaa tgtaattatt aatatatata
481 tatatttatc aatatataat ttaatataaa aaaaaaaaag aaatttaata aacatataat
541 atttataaaa tcataaattt aaaataaata atttttttaa tatttaaaaa ttctaataaa
601 aagtttttta tatttagtaa atttgtaaaa tcaatttgta acaaaaacta gtaatttaaa
661 aaaaaaaaaa acaaaaaaaa aaaaaaatga agattttatc attattagtt gtaggtgcct
721 tatgtatggg aggtaaagtt tatggtgaag ttaatggtga ttggaacaat aatggtgatt
781 ggaacaataa tggcgattgg aacaataatg gtgattggaa caataatggt ccaattttag
841 gtaagtttta ttttaaacta tatacataaa aataataaaa taaaaaaatt tggtattaat
901 actttttttt tttttttttt ttttatttat tattattaaa tcttgacttt tttatataga
961 ctgttcaaca cttcaatgtc caccaagata tcattgtgaa gtaaataatg gtaatagaca
1021 atgtgttgaa gatcaaatta cattgccacc atttgataaa tgtgataatg tccattgtcc
1081 aaaaggattt aattgcaaat atgattggga aaaagatctt gctctttgtg ttccatggag
1141 accatatcca ccagtttgta gaactagatg tccagaaggt catgaatgta aagttgatga
1201 atggggtaaa gaatgttgcg taaagatcaa atgtgatgat atttgtgact tgcgttgtcc
1261 aaagggtcat gaatgcaaga tcaaacatga tggtagtaaa tgctgtgtcc gttcatggag
1321 accaagacca cataaaccac atccacgtcc accaatctgc agattaagat gtccaccagg
1381 tcatgaatgc aaacatgatg aacatggtaa agaatgttgc gttaaaaaac gtcatcatga
1441 tagatgtgac ctcaaatgta agagaggtta tgaatgtaaa atcaaacatg atggctccaa
1501 atgttgtgtc aagagaactc caaagagacc atgttgtaaa ccaaattcat gtgccagaga
1561 tgaaaaatgt gttgccacca aagacagaat catttgtgta aaaccaactt gtgataatac
1621 tcgttgtcct ccaaattacc attgcatatg tggtgataaa attgatggtg taaaatgtgt
1681 tccagattgt aagaaagcaa gatgtgatga tgtcgaatgt ccagatttcc atagatgtgt
1741 tgaaagacgt ggtggtatct taagttgtga atttgatcca ccaagacaac caagatccct
1801 tgattgggct gaaaatgaaa atgatgatcg tgactatgat gatcgtgact atgatgatga
1861 tgaatatgat ggtgattatg atggccgtga cggtgactat gatggtgatt atgatggtga
1921 ttatgatgat gacaattatt atggtgatga tgactatgat aatgattggg acaatgataa
1981 tgattggggt aatgattggg ataatgattg ggacaatgaa gatggtgata attggaatga
2041 tgatgacttc caagatgcaa atgatgaatg ggactattaa gccattaatt ttttaaaaaa
2101 aaaataataa taatattaat acaaataatt ttttaaaaaa aaaaaaagaa aaaaaaaaaa
2161 aagaaaaaaa aaaaaaaaaa aaaaaaaata tctattaatc gttttaaata aagtatcatt
2221 acaaattata aaattaaata atcatttgtg ctttttaata ttatttattt aaattaaaca
2281 aattaatgtt taatttaata tttattcttt ttattttttt tatattttta acatggttga
2341 ttgattttta gaatggattt aaattttatt
//