GenBank-Updates@genbank.bio.net (05/18/91)
LOCUS DROADHN4 2369 bp ds-DNA INV 18-MAY-1991
DEFINITION Drosophila melanogaster alcohol dehydrogenase null4 allele
ACCESSION X04454
KEYWORDS alcohol dehydrogenase.
SOURCE Drosophila melanogaster DNA.
ORGANISM Drosophila melanogaster
Eukaryota; Animalia; Metazoa; Arthropoda; Uniramia; Insecta;
Pterygota; Neoptera; Holometabola; Diptera; Brachycera;
Cyclorrhapha; Schizophora; Drosophiloidea; Drosophilidae.
REFERENCE 1 (bases 1 to 2369)
AUTHORS Ashburner,M.
JOURNAL Unpublished (1986)
STANDARD full automatic
REFERENCE 2 (bases 1 to 2369)
AUTHORS Chia,W., Savakis,C., Karp,R. and Ashburner,M.
TITLE Adh:n4 of Drosophila melanogaster is a nonsense mutation
JOURNAL Nucleic Acids Res. 15, 3931-3931 (1987)
STANDARD full automatic
COMMENT Adh:n4 is an EMS induced Adh-null allele changing gln82 to TAG. n4
was induced on Adh:D, an EMS induced electrophoretic allele due, as
shown here, to gly232->glu232. This sequence corresponds to bases
4316-6671 of DMADHALL and all changes with respect to that sequence
are annotated in that entry.
From EMBL 26 entry DMADHN4; dated 06-JUL-1989.
FEATURES Location/Qualifiers
misc_feature 1..417
/note="5' flanking region"
promoter 418..424
/note="distal TATA box"
precursor_RNA 451..2320
/note="distal transcript"
mRNA 451..536
/note="exon 1"
misc_RNA 451..451
/note="distal CAP site"
intron 537..1200
/note="intron 1"
allele 897..936
/note="insertion 1a"
promoter 1135..1142
/note="proximal TATA box"
precursor_RNA 1167..2320
/note="proximal transcript"
misc_feature 1167..1236
/note="leader 2"
misc_RNA 1167..1167
/note="proximal CAP site"
mRNA 1201..1335
/note="exon 2"
CDS 1237..1335
/note="alcohol dehydrogenase (aa 11-33)"
/codon_start=1237
intron 1336..1400
/note="intron 2"
mRNA 1401..1805
/note="exon 3"
CDS 1401..1805
/note="alcohol dehydrogenase (aa 34-168)"
/codon_start=1401
allele 1548..1548
/note="n4 mutation (C->T)"
intron 1806..1875
/note="intron 3"
mRNA 1876..2320
/note="exon 4"
CDS 1876..2139
/note="alcohol dehydrogenase (aa 169-256)"
/codon_start=1876
allele 1949..1949
/note="F mutation (A->C)"
allele 2069..2069
/note="D mutation (G->A)"
misc_feature 2143..2320
/note="3' UT end of mRNA"
misc_feature 2269..2285
/note="poly-A signal site"
misc_feature 2321..2369
/note="3' flanking region"
BASE COUNT 726 a 544 c 475 g 618 t 6 others
ORIGIN
1 ccctacactg taagaaatta ctatagaaat aacggtacac ggaataagat atttttttta
61 gtccatatgc ttttaacaaa tgtgttttaa gtttatgtta tattattgtt agaaaaccgg
121 tgttttttta aaatcggtta aaaaattact acgagagaaa aatacaaatt ttgtaaataa
181 gattgactct ttttcgattt tggaatattt tcattcattt tatgttttta cgttttcact
241 tatttgtttc tcagtgcact ttctggtgtt ccattttcta ttgggctctt taccccgcat
301 ttgtttgcag atcacttgtt gcgcattttt attgcatttt acatattaca cattatttga
361 acgccgctgc tgctgcatcc gtcgacgtcg actgcactcg cccccacgag agaacagtat
421 ttaaggagct gcgaaggtcc aagtcatgca ttattgtctc agtgcagttg tcagttgcag
481 ttcagcagac gggctaacga gtacttgcat ctcttcaaat ttacttaatt gatcaagtaa
541 gtagcaaaag ggcacacaat tgaaggaaat tcttgtttaa ttgaatttat tatgcaagtg
601 cggaaataaa atgacagtat taaatagtaa atattttgta aaatcatata taatcaaatt
661 tattcaatca gaactaattc aagctgtcac aagtagtgcg aactcaatta attggcatcg
721 aattaaaatt tggannnnnn ggccgcatat tcctcttgga aaatcacctg ttagttaact
781 tctaaaaata ggaattttaa gataactcgt ccctgttaat cggcgccgtg ccttcgttag
841 ctatctcaaa agcgagcgcg tgcagacgag cagtaatttt ccaagcatca ggcatataat
901 atactaatac taatactaat actaatacta atataagaat actaatatag aaaaagcttt
961 gccggtacaa aatcccaaac aaaaacaaac cgtgtgtgcc gaaaaataaa aataaaccat
1021 aaactaggca gcgctccgtc gccggctgag cagcctgcgt acatagccga gatcgcgtaa
1081 cggtagataa tgaaaagctc tacgtaaccg aagcttctgc tgtacggatc ttcctataaa
1141 tacggggccg acacgaactg gaaaccaaca actaacggag ccctcttcca attgaaacag
1201 atcgaaagag cctgctaaag caaaaaagaa gtcaccatgt cgtttacttt gaccaacaag
1261 aacgtgattt tcgttgccgg tctgggaggc attggtctgg acaccagcaa ggagctgctc
1321 aagcgcgatc tgaaggtaac tatgcgatgc ccacaggctc catgcagcga tggaggttaa
1381 tctcgtgtat tcaatcctag aacctggtga tcctcgaccg cattgagaac ccggctgcca
1441 ttgccgagct gaaggcaatc aatccaaagg tgaccgtcac cttctacccc tatgatgtga
1501 ccgtgcccat tgccgagacc accaagctgc tgaagaccat cttcgcctag ctgaagaccg
1561 tcgatgtcct gatcaacgga gctggtatcc tggacgatca ccagatcgag cgcaccattg
1621 ccgtcaacta cactggcctg gtcaacacca cgacggccat tctggacttc tgggacaagc
1681 gcaagggcgg tcccggtggt atcatctgca acattggatc cgtcactgga ttcaatgcca
1741 tctaccaggt gcccgtctac tccggcacca aggccgccgt ggtcaacttc accagctccc
1801 tggcggtaag ttgatcaaag gaaacgcaaa gttttcaaga aaaaacaaaa ctaatttgat
1861 ttataacacc tttagaaact ggcccccatt accggcgtga cggcttacac tgtgaacccc
1921 ggcatcaccc gcaccaccct ggtgcacacg ttcaactcct ggttggatgt tgagcctcag
1981 gttgccgaga agctcctggc tcatcccacc cagccctcgt tggcctgcgc cgagaacttc
2041 gtcaaggcta tcgagctgaa ccagaacgaa gccatctgga aactggactt gggcaccctg
2101 gaggccatcc agtggaccaa gcactgggac tccggcatct aagaagtgat actcccaaaa
2161 aaaaaaaaaa aacataacat tagttcatag ggttctgcga acagaagata ttcacgcaag
2221 gcaataaggc tgattcgatg cacactcaca ttcttctcct aatacgataa taaaactttc
2281 catgaaaaat atggaaaaat atatgaaaat tgagaaatcc aaaaaactga taaacgctct
2341 acttaattaa aatagataaa tgggagcgg
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