GenBank-Updates@genbank.bio.net (05/18/91)
LOCUS ENHARSORI 802 bp ds-DNA INV 18-MAY-1991
DEFINITION Entamoeba histolytica ARS origin of replication (automonously
replicating sequence)
ACCESSION X52309
KEYWORDS autonomous replicating sequence.
SOURCE Entamoeba histolytica DNA.
ORGANISM Entamoeba histolytica
Eukaryota; Animalia; Protozoa; Sarcomastigophora; Sarcodina;
Rhizopoda; Lobosa; Gymnamoeba; Schizopyrenida; Tubulina;
Entamoebidae.
REFERENCE 1 (bases 1 to 802)
AUTHORS Mirelman,D.
JOURNAL Unpublished (1990)
STANDARD full automatic
REFERENCE 2 (sites)
AUTHORS Gordberg,J., Salazar,N., Oren,R. and Mirelman,D.
TITLE Autonomous replication sequences in an extrachromosomal element of
a pathogenic Entamoeba hisolytica
JOURNAL Nucleic Acids Res. 18, 5515-5519 (1990)
STANDARD full staff_review
COMMENT *source: strain=pathogenic zymodeme NIH:200; clone=R2.3; *source:
developmental stage=trophozoite; library=Lambda gt10 This sequence
is functional in autonomous replication of S. cerevisiae plasmids,
replacing ARS sequences. "ARS consensus" features refer to homology
with S. cerevisiae ARS consensus sequences. See <M11449> and
<K00909> for other ARS sequences.
From EMBL 26 entry EAARSORI; dated 01-OCT-1990.
FEATURES Location/Qualifiers
misc_feature 68..79
/note="ARS consensus"
misc_feature 75..86
/note="ARS consensus"
misc_feature 162..173
/note="ARS consensus"
misc_feature 742..753
/note="ARS consensus"
misc_feature 772..783
/note="ARS consensus"
BASE COUNT 300 a 70 c 62 g 370 t
ORIGIN
1 aagctttaga gtttcttaat caatactctc gggtattgaa tgaaacgatt tgctgagact
61 taaccgtaat ttctatttaa atttatataa aattactcat tttaaaaagg ttattctttc
121 tttttttatt tatttaatat aaatttattg atatttattg ttttttatca ttaaatattt
181 aaattaattg attgtctttc ttttttatca tttaaataaa taatatttaa tatctcaaaa
241 aatatcaact aaagtataaa tattcataag gtaaatgttg ttcataatta aattctaaac
301 atttattatt ttaatattct ttaatgattg attaattgat tgtctttctt tttttatttg
361 attgtctttc ttttttatca tttaaataaa taatatttaa tatctcaaaa aatatcaact
421 aaagtataaa tattcataag gtaaatgttg ttcataatta aattctaaac atttattatt
481 ttaatattct ttaatgattg attaattgat tgaatgtttc ttagtactat taacatttga
541 attgaaaata gtttaaataa tattatagtt ttttaatata atggatcttc ttaaaaatag
601 aaatttaaaa tagataacac ttccttcata tgtcttgaat aaaaatgtct ctactatttg
661 aatataaact attctataaa tagaacatta aataaaatat tttataatat ctcagttata
721 caacgttcag aattaataat tcttttattt ttacaaacta taaatgaatg tattttgtat
781 ttagtacttt ttattagaat tc
//