GenBank-Updates@genbank.bio.net (05/18/91)
LOCUS ONOR1G 2035 bp ds-DNA INV 18-MAY-1991
DEFINITION Oxytricha nova micronuclear DNA for R1 gene
ACCESSION X13843 Y00207
KEYWORDS R1 gene; chromosome fragmentation site.
SOURCE Oxytricha nova DNA.
ORGANISM Oxytricha nova
Eukaryota; Animalia; Protozoa; Ciliophora; Polyhymenophora;
Spirotricha; Hypotrichida; Sporadotrichina; Oxytrichidae.
REFERENCE 1 (bases 1 to 2035)
AUTHORS Klobutcher,L.A.
JOURNAL Unpublished (1987)
STANDARD full automatic
REFERENCE 2 (bases 1 to 2035)
AUTHORS Ribas-Aparicio,R.M., Sparkowski,J.J., Proulx,A.E., Mitchell,J.D.
and Klobutcher,L.A.
TITLE Nucleic acid splicing events occur frequently during macronuclear
development in the protozoan Oxytricha nova and involve the
elimination of unique DNA
JOURNAL Genes Dev. 1, 323-336 (1987)
STANDARD full automatic
COMMENT *source: strain=O. nova H10
The sequences of the micro- and macronuclear R1 genes were
compared. Several internal eliminated sequences were found within
the micronuclear sequence. These IES are removed during
macronuclear development. The sequence of the macronuclear R1 gene
can be assembled from regions 135-269, 317-541, 1535-1557,
1572-1632, and 1658-1793; see M18657.
From EMBL 26 entry ONR1G; dated 06-JUL-1989.
FEATURES Location/Qualifiers
misc_feature 134..135
/note="chromosome fragmentation site"
misc_feature complement(135..1793)
/note="micronuclear R1 gene"
CDS complement(175..269)
/note="part 6 of R1 protein (AA 271-301)"
/codon_start=269
misc_feature 270..316
/note="internal eliminated sequence 1"
CDS complement(317..541)
/note="part 5 of R1 protein (AA 195-270)"
/codon_start=541
misc_feature 542..577
/note="internal eliminated sequence 2"
CDS complement(578..986)
/note="part 4 of R1 protein (AA 59 - 194) (986 is 3rd base
in codon)"
/codon_start=986
misc_feature 987..1534
/note="internal eliminated sequence 3"
CDS complement(1535..1557)
/note="part 3 of R1 protein (AA 51-58) (1535 is 2nd base
in codon)"
/codon_start=1557
CDS complement(1552..1632)
/note="part 2 of R1 protein (AA 24-50)"
/codon_start=1632
misc_feature 1558..1571
/note="internal eliminated sequence 4"
misc_feature 1633..1657
/note="internal eliminated sequence"
CDS complement(1658..1726)
/note="part 1 of R1 protein (AA 1-23)"
/codon_start=1726
misc_feature 1793..1794
/note="chromosome fragmentation site"
BASE COUNT 688 a 338 c 324 g 685 t
ORIGIN
1 cgaagtgaac aacattaatc ttatgatcgg cagggaagag atcaagtctt tcaaattcct
61 gctctttagc caaaagtgag tagatacgat taactacata atttaatttc ttgacaaata
121 ctataattga ctccaagaga aaatggattt taacaactga ttttaaataa ttcaagaatt
181 ctttgagtaa gttgattaaa tgtattgtcc aaatccataa ggagtgatga catgtctctt
241 gaagtagaag caggcaactg cagctccaat catttataat atcgagtaac ataaaaaatg
301 aaataaagat agtgaactgc ataaaaggtg tagtaaaaga cagtgtagta gattttgtct
361 ccaaagaatt tcttgaaagc ttaaacgaaa ccctgagatg tccacatcca tttgaagtat
421 cccatgaagt actgagcgtc ctttttagac ttcttcttgg ctatctcctt tgggtttgat
481 tctttcttct cctctgaaat gatgactctt cttctctagg cattaccttg aactccatag
541 catgtattta ttaagaatta gagactaaat gagtagctaa gttgtgaatt gagaagaggc
601 tgtctaatta cttgctcatc ttcatggact tgttagcctt ctgatgatgg aatggtttca
661 tttgcggaag gagcctgatc ttattagagt tagtggaagc gagcgatgct gacatcgttc
721 tgggcgttca tctggacctc taccattgaa gtgaccatat cattagttac tcctgaatct
781 tacatttcag aagctactaa cagttgagca tcagcctaag gttgctcctc ttgcacagct
841 gggctgctaa cttcctagtt tatttgctca gcagcttctt ggcttgcgtt gcttgagcct
901 ccctcaagaa gaatggattc ttcaaggacc acttgatttt gagattagtt atcagacctt
961 aaattagaag tgttatcgct cagaatataa acttaaattt tgtctataaa tataccaaaa
1021 tcctttaaaa caaataaatg gattattaag ctaagaaaca atcaatttcc tccttagaat
1081 tccaaagata ctctagtact aaaataaaga tgagatttga tttgaaagac tgtaaatttc
1141 atacgggaat taaagcgttg agaatccttt agaattatgg aaaactttga tttcagtaaa
1201 atatggctgc tctatagcta taaattcatt ctgaactaaa agctgcaata ttccaaagag
1261 tcattataca aaattcgtaa aataatatgc aaataatttt attattttct aatttctcat
1321 acctcttgat cattgaaaat aaaaatggac cataaatctt tctatttatt aaatgattaa
1381 tctatattag attaattaac caactaaaat acaaataaac tgttaaaaaa ccgaatgagt
1441 ataaaattaa cagcattttt ttggaatatt ttaaaagaat agaatgattt gttcatttta
1501 aagaattagt ttatctcata ataaggtttt aaatgctttg ctcttgactt gatttattat
1561 aaataattta tcggggatgc tttctgaaac taaagcagaa gattcatttg aatctacctg
1621 aacgaaatca cttttaaatt aaataagtaa gatcactcta agagtttgag gcttcacctg
1681 aggaaacaat gggacttgct agttcgtctt ggttttgtga ttccatactt gaggcaataa
1741 aactattata aatttacaag caatacaatt tatcatgact taagacaacg aattgatcac
1801 ataaacgaaa attaaaggtg aagaaaatgc cataataaaa cattatacat taaactttca
1861 tgctttctat ctcgatttat attttcatag gaagatcttg tcgttatgaa tcttgaagtc
1921 ttctatcatt ctgttgttaa cttgcttgat taagtctaca tgcatctcaa gacaacctcc
1981 tacgcattca taatcgaccc tatcctccat agtctggcct tctcctttct cgcag
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