GenBank-Updates@genbank.bio.net (05/18/91)
LOCUS ECOPBPC 2936 bp ds-DNA BCT 18-MAY-1991
DEFINITION E. coli pbpA gene for penicillin-binding protein (PBP)2
ACCESSION X04516 D00001 N00001
KEYWORDS mrdA gene; pbpA gene; penicillin-binding protein;
unidentified reading frame.
SOURCE Escherichia coli DNA.
ORGANISM Escherichia coli
Prokaryota; Bacteria; Gracilicutes; Scotobacteria;
Facultatively anaerobic rods; Enterobacteriaceae.
REFERENCE 1 (bases 1 to 2936)
AUTHORS Asoh,S., Matsuzawa,H., Ishino,F., Strominger,J.L., Matsuhashi,M.
and Ohta,T.
TITLE Nucleotide sequence of the pbpA gene and characteristics of the
deduced amino acid sequence of penicillin-binding protein 2 of
Escherichia coli K12
JOURNAL Eur. J. Biochem. 160, 231-238 (1986)
STANDARD full automatic
COMMENT SWISS-PROT; P05848; YP7K$ECOLI. SWISS-PROT; P05850; YP17$ECOLI.
SWISS-PROT; P08150; PBP2$ECOLI.
*source: strain=K12; The pbpA gene is also called mrdA. The active
site serine residue of PBP2 was predicted to be Ser-330.
Data kindly reviewed (15-SEP-1987) by Matsuzawa H.
From EMBL 26 entry ECPBPA; dated 22-FEB-1991.
FEATURES Location/Qualifiers
promoter 141..146
/note="put. -35 region"
promoter 168..174
/note="put. -10 region"
RBS 296..300
/note="pot. rRNA binding site"
CDS 324..530
/note="ORF1 (AA 1-69)"
/codon_start=324
RBS 526..529
/note="pot. rRNA binding site"
CDS 537..1001
/note="ORF2 (AA 1-155)"
/codon_start=537
repeat_unit 978..995
/note="imp. inverted repeat A"
repeat_unit 1001..1020
/note="imp. inverted repeat A'"
RBS 1018..1021
/note="pot. rRNA binding site"
CDS 1035..2933
/product="penicillin-binding protein 2 (PBP2)"
/gene="pbpA"
/codon_start=1035
BASE COUNT 712 a 754 c 805 g 665 t
ORIGIN
1 cccgggcagg ctgatattct ccgcagccag actttttccg ccagacacga ctttgtagaa
61 attgttttac aaaaatggcg atgcaatctg cggcgcgggg tgggatgata gcccactttc
121 gaaagccgat tcggcgacaa ttgtcccgaa atcgcctctg gttcaggtat actgacagac
181 catttttatc tatttgattc acccaggggg aaaacttgca gggtaaagca ctccaggatt
241 ttgttatcga caaaattgat gacctcaaag gtcaggacat catcgcctta gacgttcagg
301 gcaaatccag catcaccgac tgcatgatca tctgtacggg tacgtccagc cgtcatgtta
361 tgtccattgc tgaccacgtt gtgcaggagt ctcgcgcagc gggcctgtta ccgctcggcg
421 tagaaggtga aaacagcgcc gactggattg tcgtggattt gggcgatgtg attgtccatg
481 tcatgcagga agagagccgt cgcctgtatg aactggaaaa actctggagt taatgcgtga
541 agctgcaact tgtcgccgtg ggaacgaaaa tgccggactg ggtacaaacc ggttttaccg
601 agtacctgcg tcgttttccg aaagatatgc ccttcgagct gattgaaatt ccggccggaa
661 aacgcggcaa gaatgcggac atcaagcgca tactcgacaa agagggtgag cagatgttgg
721 cggccgcagg caaaaaccgc attgtcaccc tcgatattcc aggcaagccc tgggatacgc
781 cgcagttagc cgctgagctg gaacgctgga agctggatgg tcgcgacgtc agtctactga
841 ttggcgggcc tgaagggttg tcgcctgcct gtaaagcggc ggctgagcag agctggtcgc
901 tgtcggcgct taccctcccc catccgctgg ttcgcgtgct ggtcgcagag agtctgtacc
961 gggcgtggag catcaccacc aaccatcctt atcaccgtga gtgataaggg agctttgagt
1021 agaaaacgca gcggatgaaa ctacagaact cttttcgcga ctatacggca gagtccgcgc
1081 tgtttgtgcg ccgggcgctg gtcgcctttt tggggatttt gctgctgacc ggcgtgctta
1141 tcgccaacct gtataatctg caaattgttc gctttaccga ctaccagacc cgctctaatg
1201 aaaaccgcat taagctggtg cctatcgcgc ccagccgcgg cattatctac gatcgtaacg
1261 gtatccctct ggccctcaac cgcactatct accagataga aatgatgccg gagaaagtcg
1321 ataacgtgca gcaaacgctg gacgctttgc gcagcgtggt agatctgacc gatgacgata
1381 ttgctgcatt ccgaaaagag cgcgcacgtt cacaccgttt cacctctatt ccggtgaaaa
1441 ctaacctgac cgaagtacaa gtagctcgct ttgccgtcaa tcagtaccgt tttccgggtg
1501 tcgaagttaa aggctataaa cgtcgttact atccttacgg ttcggcgttg acccacgtca
1561 tcggctatgt gtcgaaaatc aacgataaag acgtcgaacg cctgaataat gacggcaaac
1621 tggccaacta tgcggcaacg catgatatcg gtaagctggg cattgagcgt tactatgaag
1681 atgtgctgca cggtcagacc ggttatgaag aggttgaagt taacaaccgt gggcgtgtta
1741 ttcgccagtt aaaagaagta ccaccgcaag ccggacacga tatttacctg acgctggatc
1801 tcaaactcca gcaatatatt gaaacgctgc tggcgggtag ccgcgcagct gtggtagtca
1861 ccgatccgcg tacaggtggg gtgctggcgc tggtttccac gcctagttat gacccaaact
1921 tgtttgttga cggtatctcc agcaaagatt attccgcctt gttgaacgat ccgaatacac
1981 cgctggtgaa ccgcgccaca cagggggttt atcctcccgc gtctacagtt aaaccctatg
2041 tggcggtttc ggcattgagc gccggggtga tcacgcgcaa tacgacgctg tttgacccag
2101 gctggtggca actgccaggt tcggaaaaac gttatcgtga ctggaaaaaa tggggccacg
2161 ggcgtctgaa tgtcacaaga tcgctggaag aatctgcgga taccttcttc tatcaggtgg
2221 cctacgatat ggggatcgat cgcctctccg aatggatggg taaattcggt tatggtcatt
2281 acaccggtat cgacctggcg gaagaacgtt ccggcaacat gcctacccgc gaatggaaac
2341 agaaacgctt taaaaaaccg tggtatcagg gtgacaccat tccggttggt atcggtcagg
2401 gttactggac agcgacccca atccagatga gtaaggcact gatgatcctg attaatgacg
2461 gtatcgtgaa ggttcctcat ttgctgatga gcaccgccga agacggcaaa caggtgccat
2521 gggtacagcc gcatgaaccg cccgtcggcg atattcattc cggttactgg gagctggcga
2581 aagacggtat gtacggtgtt gctaaccgcc ctaacggtac ggcgcataaa tactttgcta
2641 gcgcaccgta caaaattgcg gcgaaatccg gtaccgctca ggtcttcggt ctgaaagcga
2701 acgaaaccta taatgcgcac aaaattgccg agcgtttacg tgaccacaaa ctgatgaccg
2761 cctttgcgcc atacaacaat ccgcaagtgg ctgtcgccat gattctggag aacggtggtg
2821 cgggtccggc ggttggtaca ctgatgcgcc agatcctcga ccacattatg ctgggtgata
2881 acaacaccga tctgcctgcg gaaaatccag cggttgccgc agcggaggac cattaa
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