GenBank-Updates@genbank.bio.net (05/18/91)
LOCUS ECOPOXC 1974 bp ds-DNA BCT 18-MAY-1991
DEFINITION E. coli poxB gene for pyruvate oxidase
ACCESSION X04105 M13947 M13948
KEYWORDS flavoprotein; inverted repeat; oxidase; poxB gene;
pyruvate oxidase.
SOURCE Escherichia coli DNA.
ORGANISM Escherichia coli
Prokaryota; Bacteria; Gracilicutes; Scotobacteria;
Facultatively anaerobic rods; Enterobacteriaceae.
REFERENCE 1 (bases 1 to 1974)
AUTHORS Grabau,C. and Cronan,J.E.
TITLE Nucleotide sequence and deduced amino acid sequence of Escherichia
coli pyruvate oxidase, a lipid-activated flavoprotein
JOURNAL Nucleic Acids Res. 14, 5449-5460 (1986)
STANDARD full automatic
COMMENT SWISS-PROT; P07003; POXB$ECOLI.
*source: strain=K12;
Data kindly reviewed (31-MAY-1987) by C. Grabau
From EMBL 26 entry ECPOXB; dated 22-FEB-1991.
FEATURES Location/Qualifiers
repeat_unit 56..64
/note="inverted repeat A"
repeat_unit 73..81
/note="inverted repeat A'"
RBS 164..168
/note="put. ribosome binding site"
CDS 179..1894
/product="pyruvate oxidase"
/codon_start=179
BASE COUNT 474 a 521 c 535 g 444 t
ORIGIN
1 actggcctgc tcctgccatc cgcaggggga tttggttctc gcataatcgc cttatgcccg
61 atgatattcc tttcatcggg ctatttaacc gttagtgcct cctttctctc ccatcccttc
121 cccctccgtc agatgaacta aacttgttac cgttatcaca ttcaggagat ggagaaccat
181 gaaacaaacg gttgcagctt atatcgccaa aacactcgaa tcggcagggg tgaaacgcat
241 ctggggagtc acaggcgact ctctgaacgg tcttagtgac agtcttaatc gcatgggcac
301 catcgagtgg atgtccaccc gccacgaaga agtggcggcc tttgccgctg gcgctgaagc
361 acaacttagc ggagaactgg cggtctgcgc cggatcgtgc ggccccggca acctgcactt
421 aatcaacggc ctgttcgatt gccaccgcaa tcacgttccg gtactggcga ttgccgctca
481 tattccctcc agcgaaattg gcagcggcta tttccaggaa acccacccac aagagctatt
541 ccgcgaatgt agtcactatt gcgagctggt ttccagcccg gagcagatcc cacaagtact
601 ggcgattgcc atgcgcaaag cggtgcttaa ccgtggcgtt tcggttgtcg tgttaccagg
661 cgacgtggcg ttaaaacctg cgccagaagg ggcaaccatg cactggtatc atgcgccaca
721 accagtcgtg acgccggaag aagaagagtt acgcaaactg gcgcaactgc tgcgttattc
781 cagcaatatc gccctgatgt gtggcagcgg ctgcgcgggg gcgcataaag agttagttga
841 gtttgccggg aaaattaaag cgcctattgt tcatgccctg cgcggtaaag aacatgtcga
901 atacgataat ccgtatgatg ttggaatgac cgggttaatc ggcttctcgt caggtttcca
961 taccatgatg aacgccgaca cgttagtgct actcggcacg caatttccct accgcgcctt
1021 ctacccgacc gatgccaaaa tcattcagat tgatatcaac ccagccagca tcggcgctca
1081 cagcaaggtg gatatggcac tggtcggcga tatcaagtcg actctgcgtg cattgcttcc
1141 attggtggaa gaaaaagccg atcgcaagtt tctggataaa gcgctggaag attaccgcga
1201 cgcccgcaaa gggctggacg atttagctaa accgagcgag aaagccattc acccgcaata
1261 tctggcgcag caaattagtc attttgccgc cgatgacgct attttcacct gtgacgttgg
1321 tacgccaacg gtgtgggcgg cacgttatct aaaaatgaac ggcaagcgtc gcctgttagg
1381 ttcgtttaac cacggttcga tggctaacgc catgccgcag gcgctgggtg cgcaggcgac
1441 agagccagaa cgtcaggtgg tcgccatgtg cggcgatggc ggttttagca tgttgatggg
1501 cgatttcctc tcagtagtgc agatgaaact gccagtgaaa attgtcgtct ttaacaacag
1561 cgtgctgggc tttgtggcga tggagatgaa agctggtggc tatttgactg acggcaccga
1621 actacacgac acaaactttg cccgcattgc cgaagcgtgc ggcattacgg gtatccgtgt
1681 agaaaaagcg tctgaagttg atgaagccct gcaacgcgcc ttctccatcg acggtccggt
1741 gttggtggat gtggtggtcg ccaaagaaga gttagccatt ccaccgcaga tcaaactcga
1801 acaggccaaa ggtttcagcc tgtatatgct gcgcgcaatc atcagcggac gcggtgatga
1861 agtgatcgaa ctggcgaaaa caaactggct aaggtaaaaa gggtggcatt tcccgtcata
1921 ataaggacat gccatgattg atttacgcag tgataccgtt acccgaccaa gccg
//