GenBank-Updates@genbank.bio.net (05/18/91)
LOCUS ECOPIN1 2614 bp ds-DNA BCT 18-MAY-1991
DEFINITION E. coli K12 DNA fragment for invertible-P region of the excisable
element e14
ACCESSION X01805 K03521
KEYWORDS invertase; inverted repeat; unidentified reading frame.
SOURCE Escherichia coli DNA.
ORGANISM Escherichia coli
Prokaryota; Bacteria; Gracilicutes; Scotobacteria;
Facultatively anaerobic rods; Enterobacteriaceae.
REFERENCE 1 (bases 1 to 2614)
AUTHORS Plasterk,R.H. and van de Putte,P.
TITLE The invertible P-DNA segment in the chromosome of Escherichia coli
JOURNAL EMBO J. 4, 237-242 (1985)
STANDARD full automatic
COMMENT SWISS-PROT; P03014; DNIV$ECOLI. SWISS-PROT; P09153; YEE3$ECOLI.
SWISS-PROT; P09154; YEE1$ECOLI.
From EMBL 26 entry ECPIN1; dated 22-FEB-1991.
FEATURES Location/Qualifiers
CDS <1..52
/note="unidentified reading frame P-min"
/codon_start=1
repeat_unit 41..56
/note="16 bp inverted repeat 1 involved in site specific
recombination"
RBS 528..533
/note="pot. Shine-Dalgarno sequence"
CDS 539..1417
/note="unidentified reading frame P-293"
/codon_start=539
CDS complement(962..1396)
/note="unidentified reading frame"
/codon_start=1396
misc_feature complement(1486..1492)
/note="block of seven A-residues"
misc_feature complement(1486..1533)
/note="possible transcription terminator region for urf
P-plus"
repeat_unit complement(1498..1507)
/note="11 bp inverted repeat 2'"
repeat_unit complement(1523..1533)
/note="11 bp inverted repeat 2"
misc_feature complement(1527..1528)
/note="urf P-plus stop codon"
repeat_unit 1851..1866
/note="16 bp inverted repeat 1' involved in site specific
recombination/inversion"
RBS 1920..1925
/note="pot. Shine-Dalgarno sequence"
CDS 1933..2484
/product="put. invertase"
/gene="pin"
/codon_start=1933
BASE COUNT 760 a 574 c 605 g 675 t
ORIGIN
1 atcaaatcgg atggcactgt gcaaacggct ctcgaaaacc ttggtttggg agaaggctct
61 gcattacctg ttggtgtccc ggttccgtgg ccttcagcca ctccgccaac aggctggctg
121 aaatgcaatg gtgccgcttt ttctgctgaa gaatacccgg aactggcaaa ggcttatccg
181 acaaataaat tgcctgattt acgtggtgag tttattcgtg gctgggatga cgggcggggg
241 attgatacag gtcgctctat tttaagtatt caggggtatg caacggagga tcatgctcat
301 ggattaccgt caagatccac gattgtgact gatgcaacga ttaatttcta ttttgatgaa
361 atatgggtaa atagtggcac tgacattatc aaaagaggaa acacaaacga tgccggatta
421 ccagccccgg attatggaac ctttaaaaca tataaacaat cagtggatgg tttaggtgcc
481 gcagcctcag aagcagtccg cgtaatattg catttaacta tatcgtgagg gcagcctgat
541 gcataaagca atattaaata gtgaccttat tgcaacaaag gcaggggatg ttaccgttta
601 taactacgat ggtgaaacac gggaatatat ttccacttca aatgaatatc ttgccgttgg
661 tgtcggcatt ccggcatgtt cctgtttaga tgcccctggt acacataagg ctggttatgc
721 aatctgccgt tctgcagatt ttaactcatg ggaatatgtg ccagaccatc gcggtgaaac
781 ggtctatagc accaaaactg gagaatcaaa agaaatcaaa gctccgggtg attaccctga
841 aaatacaacc actatcgccc ctttatctcc atacgataaa tgggatggtg agaaatgggt
901 gaccgatact gaagcacagc atagcgccgc agtagacgcg gcagaagcac agcgccagtc
961 actgattgat gctgcaatgg cttccattag tctgattcaa ctgaaattgc aggccggacg
1021 taaactgacg caggcagaaa caaccagact taacgctgtg ctggattaca ttgacgcggt
1081 gacggcaaca gataccagca ccgcgccgga tgtcatctgg cctgaactgc cggagcgtag
1141 gccattcaat atctggcgca ctggatgtat caaccagttc cagtgcttcc agataatcca
1201 gccacaaatt atattgtttc agctcatctt ctttaagtct tcccaatgca gccttaccct
1261 gccactgtct gctattgata aaatcagttg catcagagat aagcatctgc ttttgaaagt
1321 tcgcatctcg gacttcatca tcatgagtac ggataacctc tttaatagaa ggaggaaaaa
1381 cagaaaaatc gatcacgcct ccttcagctt gagattttaa taatgactgc cagtgttctg
1441 ccgttatttc tttagaatct ggcggaatga cgcacagcca tccataaaaa aaccaacggt
1501 atttaagcaa cagtatattt tcatcttaat acccaatagc aatccagtta tagtaataac
1561 tacccgtttg agctgcgcac cgtaataaaa acgaagtggt tgtatatccc cccgttgcca
1621 ctgcattgat aggtgcgctg acattgtcat aatttgcaac aacggcaaca caggcattat
1681 taaacgcaac cgggaataca actgtatttc catccccccc agaggcagat gcttttgcgg
1741 cattccaccc ccactgaatg attactgatt gctcaatacc accaatcata accggtatag
1801 ctatgaaacc ggtgcgtcct aatgtagccg ttgctgcatt gagttttgct ccttctccca
1861 aaccaacgtt tatgaaaatg aagaaataac aagcaaatgg catcattcct gcttttacca
1921 gggggattta acatgcttat tggctatgta cgcgtatcaa caaatgacca gaacacagat
1981 ctacaacgta atgcgctgaa ctgtgcagga tgcgagctga tttttgaaga caagataagc
2041 ggcacaaagt ccgaaaggcc gggactgaaa aaactgctca ggacattatc ggcaggtgac
2101 actctggttg tctggaagct ggatcggctg gggcgtagta tgcggcatct tgtcgtgctg
2161 gtggaggagt tgcgcgaacg aggcatcaac tttcgtagtc tgacggattc aattgatacc
2221 agcacaccaa tgggacgctt tttctttcat gtgatgggtg ccctggctga aatggagcgt
2281 gaactgattg ttgaacgaac aaaagctgga ctggaaactg ctcgtgcaca gggacgaatt
2341 ggtggacgtc gtcccaaact tacaccagaa caatgggcac aagctggacg attaattgca
2401 gcaggaactc ctcgccagaa ggtggcgatt atctatgatg ttggtgtgtc aactttgtat
2461 aagacgtttc ctgcagggga taaataaagt taaagacact ttgtgtacaa aagaaagtaa
2521 aacaacagcc aacttgttcg aattttatca ataaaagtca gtattgtcgt gaaaattgat
2581 taaagttaat attatcgatg tttttgatac tatg
//