GenBank-Updates@genbank.bio.net (05/18/91)
LOCUS ECOPHOC 522 bp ds-DNA BCT 18-MAY-1991
DEFINITION E. coli gene for alkaline phosphatase (phoA), N-terminal portion.
Also contains an unknown reading frame, C-terminal portion. phoA
has a signal peptide of 21 amino acids.
ACCESSION V00315 J01659
KEYWORDS signal peptide; unidentified reading frame.
SOURCE Escherichia coli DNA.
ORGANISM Escherichia coli
Prokaryota; Bacteria; Gracilicutes; Scotobacteria;
Facultatively anaerobic rods; Enterobacteriaceae.
REFERENCE 1 (bases 1 to 522)
AUTHORS Kikuchi,Y., Yoda,K., Yamasaki,M. and Tamura,G.
TITLE The nucleotide sequence of the promoter and the amino-terminal
region of alkaline phosphatase structural gene (phoA) of
Escherichia coli
JOURNAL Nucleic Acids Res. 9, 5671-5678 (1981)
STANDARD full automatic
COMMENT SWISS-PROT; P00634; PPB$ECOLI.
From EMBL 26 entry ECPHOA; dated 25-FEB-1991.
FEATURES Location/Qualifiers
CDS <1..179
/codon_start=3
/note="unknown reading frame"
/codon_start=1
mRNA 242..>522
/note="messenger RNA"
CDS 283..>522
/product="alkaline phosphatase"
/gene="phoA"
/codon_start=283
BASE COUNT 139 a 112 c 132 g 139 t
ORIGIN
1 aagctttgga gattatcgtc actgcaatgc ttcgcaatat ggcgcaaaat gaccaacagc
61 ggttgattga tcaggtagag ggggcgctgt acgaggtaaa gcccgatgcc agcattcctg
121 acgacgatac ggagctgctg cgcgattacg taaagaagtt attgaagcat cctcgtcagt
181 aaaaagttaa tcttttcaac agctgtcata aagttgtcac ggccgagact tatagtcgct
241 ttgtttttat tttttaatgt atttgtacat ggagaaaata aagtgaaaca aagcactatt
301 gcactggcac tcttaccgtt actgtttacc cctgtgacaa aagcccggac accagaaatg
361 cctgttctgg aaaaccgggc tgctcagggc gatattactg cacccggcgg tgctcgccgt
421 ttaacgggtg atcagactgc cgctctgcgt gattctctta gcgataaacc tgcaaaaaat
481 attattttgc tgattggcga tgggatgggg gactggggat cc
//