GenBank-Updates@genbank.bio.net (05/18/91)
LOCUS LEPSPHC 3987 bp ds-DNA BCT 18-MAY-1991
DEFINITION Leptospira interrogans sphingomyelinase-c gene (EC 3.1.4.12)
ACCESSION X52176
KEYWORDS hemolysin; sphingomyelinase; sphingomyelinase-c;
unidentified reading frame.
SOURCE Leptospira interrogans DNA.
ORGANISM Leptospira interrogans
Prokaryota; Bacteria; Gracilicutes; Scotobacteria; Spirochetes;
Spirochaetales; Leptospiraceae.
REFERENCE 1 (bases 1 to 3987)
AUTHORS Segers,R.P.
JOURNAL Unpublished (1990)
STANDARD full automatic
REFERENCE 2 (bases 1 to 3987)
AUTHORS Segers,R.P., Van,d.D.A., De,N.A., Corcione,P., Van,d.Z.B.A. and
Gaastra,W.
TITLE Molecular analysis of a sphingomyelinase-c gene from Leptospira
interrogans serovar hardjo
JOURNAL Infect. Immun. 50, 2177-2185 (1990)
STANDARD full automatic
COMMENT SWISS-PROT; P17627; PHL$LEPIN. SWISS-PROT; P17628; Y348$LEPIN.
*source: strain=sponselee, serogroup sejroe, serovar hardjo;
*source: library=lambda EMBL3; clone=pHL2-B3, pHL2-B4;
From EMBL 26 entry LISPHC; dated 25-FEB-1991.
FEATURES Location/Qualifiers
promoter 528..533
/note="pot. -35 region"
promoter 559..564
/note="pot. -10 region"
RBS 570..574
/note="pot. ribosome binding site"
CDS 581..2248
/note="precursor protein (AA -27 to 529)"
/codon_start=581
CDS 581..661
/note="signal peptide (AA -27 to -1)"
/codon_start=581
CDS 662..2248
/note="mature sphingomyelinase-c (AA 1-529)"
/codon_start=662
repeat_unit 1337..1365
/note="inverted repeat A"
repeat_unit 1632..1755
/note="inverted repeat C"
repeat_unit 1698..1724
/note="inverted repeat B"
repeat_unit 2268..2297
/note="inverted repeat D"
repeat_unit 2315..2347
/note="inverted repeat E"
CDS complement(2437..3345)
/note="ORF 1 (AA 1-303)"
/codon_start=3345
promoter complement(3277..3281)
/note="pot. -10 region"
repeat_unit 3332..3370
/note="inverted repeat F"
RBS complement(3354..3357)
/note="pot. ribosome binding site"
promoter complement(3394..3399)
/note="pot. -35 region"
CDS complement(3398..>3987)
/note="ORF 2 (incomplete) (197 AA) (3987 is 3rd base in
codon)"
/codon_start=3987
BASE COUNT 1205 a 685 c 746 g 1351 t
ORIGIN
1 ggatcctttt ttcgttattt ttactaagaa tttgttccaa ggacgtggaa acttttacgt
61 tttagatgtt cgtggataga acttttttgc tcctaatttt gttcatcaag tagcttgatc
121 tttgtaaatc tgatttgaga actttttaca ttacatacaa gtccataaaa cgccatatat
181 gcttgaagtc gatttctaat ttcttgtgga acgatttttg aaatgatgaa atatatatta
241 tatttttaat ataatatagc ttataaaagc cgtttaatct gcccggtgtg ggatttcaaa
301 atcataaaag attagaaatc gtttttatat gacatacgat tatcaaattt cttcatttaa
361 tcttgaaaca tgatctaaaa agtaaacaaa gactttgttc gcttttaatt tgtttatttt
421 tatacattta aaattttgaa ggttgcttta ggaattggca tttttaattt caattacttg
481 agcgttatat gaaattgatt cgtttttata aaatactctt cttcttgttg aactctatgt
541 tatagtatgg gcattgattt aaaaagaatg gagatagtgt atgagaataa aaaaatatac
601 aaaagtgaga cttcttgtaa attgttgtct cttattattt tttctaatag attgtggagc
661 cgatagacaa tctttgtata aagatttact tgcgtctcta atttacattt cagataataa
721 aaacattggt tctacaaatt ctgatttaac gggttctggc tctgtaagtt ctagtcccgc
781 agatgccgct cctgagaatt ccatccttgc gaattctatt cctgagaaca tgggaattaa
841 aattctaact cacaacgttt tcctgttgcc gaaaacgctt ccaggttggg gaaattgggg
901 acaaaacgaa agagcacaac gtatcgtgag ttcgaattat atccaaaacc aagacgtcat
961 tgtgttcgat gaagcttttg atacagatgc aagaaaaatt ctcttggacg gagttcgttc
1021 agaatatccg tatcaaaccg atgtgatcgg aagaactaaa aaaggttggg atgctactct
1081 tggcttgtac agaacggatg cgtttacgaa cggaggagtt gttattgtaa gtaaatggcc
1141 catcgaagag aaaatacaac atgtcttcaa agaaaagggc tgtggcgcgg atgttttttc
1201 caacaaagga tttgcttatg tgaggatcga taaaaacgga agaaagtttc atatcattgg
1261 aactcatgta caggcacaag attccggatg tgcgaactta ggagtcgtct caagagtgaa
1321 tcaattcaac gagatcaggg attttatcga ttccaaaaaa atcccgaaaa acgaaatggt
1381 tctgatcgcg ggagatttga acgtaatcaa aggtagtaga gaatatcatc agatgctttg
1441 tatattgaac gtaaacaatc ctaaatatgt aggagttcct tttacgtggg atacaaaaac
1501 caatgaaatt gccgcttttt attataaaaa agtggagccc gcatacttgg attacatatt
1561 cgtttcaaag tcccactttc aaccaccgat ttggcagaat ttagcttacg atccaatctc
1621 tgcaaaaact tggaccgcaa agggatatac gagtgatgaa ttttccgatc actatcccgt
1681 ttacggtttt atctatgcgg attcatccac tccgacgaag tccggacgta aaaggaaata
1741 cgatcgagtt tccttcgtat ctgtagccac tggaaaaaag attcaagcta attctgaaaa
1801 atcgaacgca tggttgaagg tgaatgctac gacggaaacc gatttaacaa aattcaattt
1861 ggtgcaaacg aatgatcccg attcgaatcc ctcctgcatg aaaagcgggc atgtccggat
1921 cgaatcttct cattctttaa attacttttg gaattggtgg ctcggaggag gtaaaggtaa
1981 ctacgcgtac tatcccaaat tcaacgacgg gtcgaatcga atccaaatca taaacttaga
2041 cggagggtgt ttgcaagacg ggagtcgagt cgcattcaaa gattatgata ctatttcaag
2101 aagacaatat tttctcactg tttgggaagg tggaaactgg gataaatatc tctatctttg
2161 gagaagccat atcggtctaa gggaaatttt ttatctcaag ctggattcct ctcccgaaat
2221 gaattggagt aagaaattaa tttatcgttg agccttaaaa atcaaatgaa agacggcagg
2281 gatgtccctg cctcttttcg cgtacataga atttttatat cgatccagga ctttgcgata
2341 gatataatga ggctctaatt ctaattttat tactcggacg tatgaaaaaa ttccatacag
2401 gaattagtgg taaaagaagc cttcggtttg atcttacagc ttcaaccctc cgccgatcat
2461 aaagatcggt tcgttaaaat gataagattg tgtcgcccca attaaatctg tttttcggta
2521 ttgtagagct atattaaaaa agtaagattc tagttcccat tgaatgcccg ttcccacaaa
2581 aaggaccgtt tgtactttgg aatgtactcc actttcaata ctttttccga ctcccaagcc
2641 tacttgagtg aaccaaaaaa tagactcgga aagaggaaaa taatatttca ttcccggcaa
2701 aagtgaaaga gtttctatac cgaatttatc gttcccttgt ttatcaaaat taaaatttcc
2761 ttcaaacaga aataaattta tggaaatgct ggataacgtt tggtattccg gggtagagtt
2821 aaaattagag ttcataagaa ttccaacaag aggagaaatc ttgcgatccg gaaaagaata
2881 agagtttagt tcgattccga ataaaaagct gagattcaaa ttgtaacgat attcgccatt
2941 aaaggaagtt gttttggtat ttacagtata tcttttctca atataagggt ctatatagat
3001 tacgcccgtg ataaggtccg tgatgttgta tccgcggttg aaattcatct tttccgtcac
3061 tttggtgctc caacttgaca aattccaaga aaaatagaat tttttaatat tattatgttc
3121 tttttgtttc tcttcgcctt ccggcttttt gatttccgaa acaagaattt tttcaaccag
3181 agttaaatcg attttttcga cttttcccgt ttcgtcctcg actaagacgt gatcctcccg
3241 cagtgaagtt ctaacgtttc ccatttttct tccgtctttt agaatcaaag tgtctccaaa
3301 aatccagatc ggatctaata ggaggcatat acaaaaaatt tccatgagcg aaactcccgg
3361 aaactttttg aaaaagtgat ttgagtttag gatcttcaaa atgaaatcga ctctatattt
3421 gtggattcga tgattctttt tttttcgttt tcttccaaga tatattggct tccgtattgc
3481 attgcatttc ctttaagtat cgttccgtct ttgagttgaa tcgttttatt tcctaaacgc
3541 aaaggttttt cttggttgct ttcggtattt gtggtggtcg aagtttttct ttcgatcggt
3601 tcctgttttt tctctatctc cgagagtttt ataggttcag aatttccaag ttgatttgaa
3661 atattttcta gatctttgag atacaactca ttcaacggag cttgtttttt ttgcgagacg
3721 ttctttatga tttgttgttg aatctctttt agaatattca aattttcttg tgcgcttttt
3781 gcatcaagtc gatcttcttt caaagtctta aataaacttt tttcccgact tcccgataga
3841 tttagaggtt ttatttcaaa ataagaatta gagatatctt ccgaagaagt gtccacccat
3901 actttttcca attcggtctt ttgcccgaag tttgaacccg tttgtttatt caaagaatgt
3961 tctatcaact cttccttttg gggatcc
//