GenBank-Updates@genbank.bio.net (05/19/91)
LOCUS BACMEU 1615 bp ds-DNA BCT 19-MAY-1991
DEFINITION Bacillus sphaericus restriction modification methylase gene
ACCESSION X15758 M29331
KEYWORDS dna binding protein; methylase; restriction modification enzyme.
SOURCE Bacillus sphaericus DNA.
ORGANISM Bacillus sphaericus
Prokaryota; Bacteria; Firmicutes; Endospore-forming rods and cocci;
Bacillaceae.
REFERENCE 1 (bases 1 to 1615)
AUTHORS Posfai,G., Kiss,A., Erdei,S., Posfai,J. and Venetianer,P.
TITLE Structure of the Bacillus sphaericus R Modification Methylase Gene
JOURNAL J. Mol. Biol. 170, 597-610 (1983)
STANDARD full automatic
COMMENT SWISS-PROT; P13906; MTB1$BACSH.
*source: clone=pES2;
From EMBL 26 entry BSMET; dated 25-FEB-1991.
FEATURES Location/Qualifiers
promoter 68..73
/note="-35 region"
promoter 91..96
/note="-10 region rbs"
precursor_RNA 103..>102
/note="primary transcript"
RBS 117..124
/note="pot.ribosome binding site"
CDS 132..1403
/product="methylase"
/codon_start=132
RBS 202..205
/note="pot.ribosome binding site"
CDS 216..1403
/note="alternative translation methylase"
/product="methylase"
/codon_start=216
repeat_unit 1415..1427
/note="inverted repeat A"
repeat_unit 1435..1447
/note="inverted repeat A'"
BASE COUNT 565 a 250 c 355 g 445 t
ORIGIN
1 atgaacgata atagtatctt agacccatca attgtttgta aaaaaaaacg ttagcaaact
61 ttacatattg tcacgacgat gaagttccgt taaacttaag ggatgagaat tttgtcgaaa
121 ggggcaacaa catggctatt aagataaatg aaaaaggcag aggtaagttc aagccagctc
181 ctacctatga aaaagaagaa gtaagacaac tattgatgga aaaaatcaat gaggaaatgg
241 aggctgttgc tactgcaaca agtgatattt ccaatgatga aattcaatat aaatcggata
301 agtttaatgt tctttctttg ttctgtggag caggaggatt agacctaggt tttgaacttg
361 ctggtctaga acaaagcctt ggaacggata aagcactaga agcgtttaaa gatcgagacg
421 tatataatgc aattcggcat gaaagtgttt tccatactgt ctatgcgaat gatattttta
481 gtgaagcctt acaaacctat gaaaagaata tgcccaacca tgtgtttatt catgaaaagg
541 atatacgaaa aataaaagaa tttccatctg cgaatcttgt tatcggtgga tttccttgtc
601 cagggttttc tgaggcagga ccaagacttg tggatgatga gagaaacttt ttatacatac
661 acttcatccg ttgcctcatg caagtacaac ctgaaatttt cgtcgccgaa aatgtaaaag
721 ggatgatgac ccttggaggc ggagaggtat ttagacaaat tgtagaggat ttcggtgcag
781 cgggttaccg tgtggaagca aggcttttaa acgcaagaga ctacggcgtt ccgcaaatta
841 gagaacgtgt gataatcgtt ggggttagaa acgatataga tttcaactat gaatacccag
901 aaattacaca tggaaacgaa gaaggattaa aaccatatgt gacgctagaa gaggcgattg
961 gtgacttgag tttagaccca ggaccttact ttacagggag ttattcgacg atctttatga
1021 gtcgtaatcg gaaaaagaaa tggactgacc agtcctttac gatacaagca agtggtcgac
1081 aggcaccgat tcacctaggt ggtcttccaa tggagaaagt ggataaaaac aaatggattt
1141 tccctgatgg cgaagagaac catcgtagac tttctgttaa agaaattaag cgaatacaaa
1201 catttcctga ttggtatgaa tttagtgatg gtggcaacat gaaagtgtct gttaataatc
1261 gattggacaa gcagtataaa caaattggaa atgcagtacc tgtctttttg actcgtgcgg
1321 ttgctaagtc cattgctcag tttgcagcag attatttgaa agataatcat cctcatgaag
1381 caccgcaaat gaaattattt atttaggtat gactaaaaat accatccatc aataggatag
1441 tattttttta gtaggaaaat gatctcgcaa tcacgaatta aaatatctta gacaggagag
1501 gtaaaacatg aatgaacagc aaacattgga tttaacgaac attcatataa caaatgtaaa
1561 ggggaaacct tttcgaaatg atttatatga tgtgaaattt gaactaaatg ggaaa
//