GenBank-Updates@genbank.bio.net (05/22/91)
LOCUS HRSIFNB 2467 bp ds-DNA MAM 22-MAY-1991
DEFINITION Horse interferon-beta gene, complete cds.
ACCESSION M14546
KEYWORDS beta-interferon; interferon.
SOURCE Horse liver DNA, clone lambda-Eq-beta-6.
ORGANISM Equus caballus
Eukaryota; Animalia; Metazoa; Chordata; Vertebrata; Mammalia;
Theria; Eutheria; Perissodactyla; Equidae.
REFERENCE 1 (bases 1 to 2467)
AUTHORS Himmler,A., Hauptmann,R., Adolf,G.R. and Swetly,P.
TITLE Molecular cloning and expression in Escherichia coli of equine type
I interferons
JOURNAL DNA 5, 345-356 (1986)
STANDARD full staff_review
COMMENT Draft entry and computer-readable sequence for [1] kindly provided
by R.Hauptmann, 23-FEB-1987.
Polyadenylation signals are located at positions 1446-1451 and
1516-1521. A Goldberg-Hogness box is found at positions 593-599.
FEATURES Location/Qualifiers
CDS 697..1257
/note="interferon-beta precursor"
/codon_start=697
sig_peptide 697..759
/note="interferon-beta signal peptide"
/codon_start=697
mat_peptide 760..1254
/note="interferon-beta"
/codon_start=760
misc_feature 2440..2467
/note="putative VECTOR sequence Bacteriphage M13mp18
(M11454)"
BASE COUNT 720 a 563 c 556 g 628 t
ORIGIN 1 bp upstream of HindIII site.
1 aagcttcatt cctagttttc agttatattg tagatagttg agattgccag ataaagcaac
61 aaatgtggct gagaaagcta tgtgatgtct tgctgtacat ggttggggcc ctacaaaaaa
121 aatttagata tgcttagttg aacattattc gatgctagat acaaaaaagt aggttgtctt
181 ttttaaaatc ataaaatgca tatatttatt gttttgtgat agtgtaattg ggaattaaat
241 ctaattctta tgaaaaagaa aattcccata caaagacccc tcaaaaacat ctcataatac
301 taaaacaaaa aaataaaaat tactttgcca aatgacagaa ctctgtaata gcagagtcct
361 aaatgattta gcttattttg ttccttggta tttaacaata cagtgacact ttacaaaaca
421 ttagaaatcc tcagagtttg tatgttttcc cctaatatac ataaaataaa ataggacttt
481 aaggatacag agttttagag actacaaata atgtaaatga cataggaaaa cagaaaggga
541 gaactgaaag tgggaaattc ctctgaaata gaaagagtgg aggaccatcc cgtataaata
601 gcccacactc acggaggaag gacatttaag ctcaagccgt tgccacctcc acttgggctc
661 ctagggagta aaggcaacac tgttcctgtc ttcatcatga cctacaggtg gatcctccca
721 atggccctcc tgctgtgttt ctccaccacg gctctttctg tgaactatga cttgcttcgg
781 tcccaactaa gaagcagcaa ttcagcatgt ctgatgctcc tgcggcagtt gaatggagcc
841 cctcaacgtt gccccgagga cacaatgaac ttccaggtcc ctgaggagat tgagcaagca
901 cagcagttcc agaaggagga tgctgcattg gtcatctatg agatgctcca gcacacctgg
961 cgtattttca gaagaaattt cgctagcact ggctggaatg agaccatcgt taagaacctc
1021 cttgtggaag tccatctgca gatggaccgt ctggagacaa acctggagga aataatggag
1081 gaggaaagct ccacctgggg aaacacaacc attctgcgcc tgaagaaata ctacggaagg
1141 atctcgcagt acctgaaggc caagaagtac agccactgtg cctggacagt ggtccaagcg
1201 gaaatgctca ggaacttggc cttccttaac ggactcacag attacctcca aaactgagga
1261 tctcccagcc tgcacctcgg agaagggaca atgctgacag tgactgcagg tgtcttccca
1321 gcagaggctc ttgacgtgac tgacagctaa ggcactgcat tggaaaggac agttacagac
1381 tttacatttt ttactaactt atgaattaac ttatttttct atttatttca acatttacct
1441 tggaaaataa attttttatg aaacaaaatt caacacggct gttttaattt caacttgatt
1501 tatagaatca cccagattaa aaactgcaaa ccacctgtaa aatgttcttt gtaaaatgtg
1561 cctgcaaact agtatagttt ctggcccctg ccttcaagga atttaaaatc caaggaagcc
1621 atgcggaata tacaagataa gaggtgagaa ggggacctca accgtacagg agaagaaatg
1681 tggcttgagc cccatataaa cggaattaaa atgggagaga caggcagagg ctctggactc
1741 agaggacggg gctgctgctt ctgccctgtg tcccgctctc tggccccaca gttagaatct
1801 gatggctctc agggtgccca gaggaatatg tcagctcttg cgtttgcctg gagctcatcc
1861 ctactatctg cgagatgctc tgcctccccc cacccctcaa ccccacagga ttgtaaaata
1921 tttctgtgcc ctgcaagcct aagcgggaga agtcccaggc acttctggga cactgtaagt
1981 ggcagtccct ttatggtact cttcttggga caaccgagct gtacaggtgt ctaaggggag
2041 ccagcgtctc tgtctccttc cagggcacag acacaagagg aagaaagaac tctgtttcat
2101 acccctgcca tcggcctggt tttgctccct atttttccag agaagcaagt ctgctccggc
2161 ttttcctgct gctctgcgtc tccaggccac actctcccca aagccaaggc caaggcaggt
2221 gcagcagatt aggtccagtc tcaggccagt gagaaaccgg gaagcatggg agacaaggaa
2281 attcaggtgg gatagagagg gcactaacgt tcccagggct tacactggga aattggagat
2341 ttcctaggag ctctttgggc accggccagc atagctgctt ttctgtctgt gctgaaccct
2401 tgggaacgtg cattattatg cctgttcttg ccatgagcag gggatccgtc gacctgcagc
2461 caagctt
//