GenBank-Updates@genbank.bio.net (05/22/91)
LOCUS CLOAPUX 4839 bp ds-DNA BCT 22-MAY-1991
DEFINITION C.thermohydrosulfuricum alpha-amylase-pullulanase (apu) gene,
complete cds.
ACCESSION M28471
KEYWORDS alpha-amylase-pullulanase.
SOURCE C.thermohydrosulfuricum (strain DSM 3783) DNA, clone EII2.
ORGANISM Clostridium thermohydrosulfuricum
Prokaryota; Bacteria; Firmicutes; Endospore-forming rods and cocci;
Bacillaceae.
REFERENCE 1 (bases 1 to 4839)
AUTHORS Melasniemi,H., Paloheimo,M. and Hemio,L.
TITLE Nucleotide sequence of the alpha-amylase-pullulanase gene from
Clostridium thermohydrosulfuricum
JOURNAL J. Gen. Microbiol. 136, 447-454 (1990)
STANDARD full staff_review
COMMENT Draft entry and computer-readable sequence for [1] kindly submitted
by H.Melasniemi 29-SEP-1989, for release after publication.
FEATURES Location/Qualifiers
misc_signal 118..123
/note="-35 region"
misc_signal 134..139
/note="-10 region"
misc_signal 141..146
/note="-10 region"
misc_binding 304..311
/note="ribosome binding site"
CDS 319..4746
/note="alpha-amylase-pullulanase precursor (gtg start
codon)"
/codon_start=319
sig_peptide 319..411
/note="alpha-amylase-pullulanase signal peptide"
/codon_start=319
mat_peptide 412..4743
/note="alpha-amylase-pullulanase"
/codon_start=412
BASE COUNT 1739 a 741 c 1006 g 1353 t
ORIGIN
1 tttagcagaa aaagcagata aaatggagat aacacaaagg ttaaagcaga tataatttat
61 agggctgctt tatagtagcc ctataaattt acatatagtg tatgtattaa caacatattg
121 acaacgaaaa aactataatt tataatagac atgagggaaa acgattgcgc aaaaatttgc
181 acaaataaaa tgtgttttta tcacatttat ctttttgcaa tttgtgaaag cgattgccta
241 aaagaaatgg gggtgggatt gggaattaat ttttttaaaa acatttaagt aaatatatgg
301 ataaaagggg gattataggt gtttaaaagg agagctttag gatttttgct ggcttttctt
361 ttagttttta cagcagtatt tggctcaatg cctatggaat ttgcaaaggc tgagacagat
421 acagcgccag cgatagccaa tgttgttggc aattttcaat ccaaacttgg agattctgac
481 tggaatataa acagtgacaa aacgataatg acatataaag gtaatggctt ttatgaattt
541 actaccccag ttgcgttacc tgcaggtgat tatgagtata aagttgctct taatcattca
601 tgggaaggtg gaggagttcc ttcacaaggt aatttaagct ttcatcttga ttcagattct
661 gtagtgactt tttattacaa ttacaacact tcaagtatta ccgattccac aaaatataca
721 ccaattcccg aagacaagct tccaagactt gtagggacta tacagcctgc aataggagca
781 ggtgatgatt ggaaacctga aacatcgact gctataatga gagactataa gtttaataat
841 gtttacgaat acactgcaaa tgttccaaaa gggaattatg agtttaaagt gactttaggg
901 ccctcatggg atataaatta tggcttaaat ggtgaacaaa atgggccaaa tattcctttg
961 aatgtagcct atgatactaa gattacattt tactatgatt cggtttcaca taatatatgg
1021 acagattata atccacctct tacaggacct gataataaca tatattatga cgatttaaga
1081 catgacaccc atgacccatt cttccgctcg cctttcggtg caataaaaac gggagatact
1141 gtgacattaa gaatacaagc aaaaaatcat gacattgagt cagctaaaat ttcttattgg
1201 gatgatatta aaaaaacaag aatagaagtc cctatgtaca gaattggtca aagtcctgac
1261 gggaaatatg aatactggga agttaagtta agctttgacc atcccacaag aatttggtat
1321 tactttatac ttaaagacgg gaccaaaact gcttattacg gagataacga tgaacaatta
1381 ggtggcgtag gtaaagccac agatacagaa aataaggact tcgaacttac tgtctacgat
1441 aaaaacttag acacccctga ttggatgaaa gggtcagtaa tgtatcaaat attccctgat
1501 aggttcttta atggggattc ttcaaatgac catctaaaga aatacagcag aggttttgat
1561 cctgttgaat atcatagcaa ctggtacgag cttccagata atccgaatga taaaaataaa
1621 ctaggatata caggggatgg catatggtca aatgacttct ttggcggtga tttaaaaggt
1681 atagatgata aattggatta tttaaaaagc cttggaatat cagtcattta tctcaatcca
1741 atttttcaat caccttctaa tcacaggtac gatacaaccg attacacaaa aatagacgaa
1801 ctattaggag atttatctac ctttaagaag ctcatggagg atgcccatgc aaaagggatt
1861 aaggtaatac ttgatggcgt cttcaatcat acaagtgatg acagcattta ttttgataga
1921 tatggtaaat acttaaacac gggagtttta ggtgcttatc aagcatggaa acagggagat
1981 cagtcaaagt ctccatatgg tgactggtat gagataaagc ctgacggtac ctatgaagga
2041 tggtggggat ttgacagttt acctgtgata aggcagataa acggcagtga atataatgtc
2101 aaaagttggg cggattttat tataaataat cctaatgcaa tatctaagta ttggttaaat
2161 cctgatgggg acaaaaatgt aggtgcagat ggttggagat tagatgttgc gaatgaagtt
2221 gctcacgatt tttgggttca ttttagaggt gcaattaata ctgtgaaacc gaatgctccg
2281 atggttgcag aaaactggaa tgacgcttca ctggatctgc ttggagattc ttttaactct
2341 gttatgaatt atctctttag aaatgcagta attgacttta tattggataa atcatttgat
2401 gacggaaatg tggttcacaa tcctatagat gcagcaaaac tcgaccaaag gcttatgagc
2461 atatatgaga gatatcctct tccagtgttt tattctacta tgaatctttt aggttctcat
2521 gacaccatga ggatattgac agtatttgga tacaactctg cggatgaaaa tcaaaattct
2581 caagcagcaa aagaccttgc ggttaaaaga cttaaacttg ctgcaatact gcaaatgggt
2641 tatccaggca tgccttctat ttattacgga gatgaagcag gacaatctgg cggaaaagac
2701 ccggacaaca gaaggacatt cccttgggga agagaagata ctgatttgca aacttttttc
2761 aagaaagttg taaatataag aaatgagaat caagttttga aaacaggaga ccttgagaca
2821 ctttatgcaa atggcgatgt ttatgccttt ggaagaagaa tcataaatgg aaaagatact
2881 tttgggaaat cgtatcctga cagtgtagct attgtcgtaa taaataaagg tgacgcaaaa
2941 caggtttcta tagataccac taaatttata agagatggag ttgcttttac agatgcctta
3001 agtggcaaga catacacggt tcaggatggt aaaattgttg tagaagttgg atcaatggat
3061 ggagctatac ttatatcaga tacaggacaa aatttgacag cacctcagcc aataacagac
3121 cttaaagcag tctcagggaa tggcaaagta gacctttcct ggagtgtagt agataaagca
3181 gtaagctata atatttaccg ttctacagtt aaaggtgggt tatatgaaaa aatagcttca
3241 aatgttacgc aaattaccta tactgataca gaggttacca atggtctaaa gtatgtgtat
3301 gctgtaaccg ctgtagataa tgatggaaat gagagtgctt taagcaatga agttgaggca
3361 tatccagcat ttcctattgg ctgggcggga aatatgaatc aagtaaatac acatgtaata
3421 ggtgtaaata atccagttga agtgtatgct gaagtgtggg ctcaagggct tacagataaa
3481 cctggccaag gagaaaatat gattgcccag ttaggatata ggtatattgg agatactgta
3541 ggagatgcag tttacaatgc cgtgtacaat aaggtagaag gtgttgaaat aagtaaggac
3601 tggacatggg ttgatgcaca atatgtaggt gattctggaa ataatgataa atacatggct
3661 aaatttgtac ctgatatggt aggcacatgg gaatacatta tgagattttc ttctaaccaa
3721 ggccatgatt ggacgtatac aaaaggacca gatgggaaaa cagatgaagc aaaacagttt
3781 actgtcgtgc catcaaatga tgtagaaaca cctacagctc cagtcttaca acaaccagga
3841 attgaatcct caagagttac acttaactgg agtccgtcag ctgatgatgt tgctattttc
3901 ggctacgaaa tctataagtc ttcaagtgag acaggaccat ttataaagat tgcaactgtg
3961 tctgacagtg tgtataacta cgtagataca gatgtagtaa atggcaatgt gtattattat
4021 aaagttgtgg cagttgatac ctcctataat aggacagcat caaatacagt aaaagcgaca
4081 cctgatataa tacctataaa agtgacattt aatgtcacaa taccagatta tactcctgat
4141 gatggtgtaa atattgctgg aaacttcccg gatgctttct ggaatccaaa tgctaatcaa
4201 atgacaaaag ctggttctaa cacctatagt attacactta ctttaaatga gggtacacaa
4261 attgaatata aatatgcaag ggggagctgg gataaagtag aaaaaggtga atacggcaat
4321 gagattgaca acagaaaaat aactgttgtc aatcagggtt caaatacaat ggttgtaaat
4381 gatacagtgc aaagatggag agatgtgcca atatatatct attctccaaa agacaagact
4441 attgttgatg caaatacgag tgaaatagag attaaaggca atacttacaa aggtgcgaaa
4501 gtaactataa atgatgaatc ttttgtgcaa caagaaaatg gagtatttac aaaagtagtt
4561 cccttggaat atggtgtaaa tactatcaaa atacatgtag agccaagtgg tgacaagaat
4621 aatgaactta caaaagatat aacaataact gttacaagag agaagccggc caggagacag
4681 aacctactcc tactccacca acagaaacaa caaaaccatc tcaagaagta tcacaaggca
4741 aaatagttgt agaaaataac acaacaacac ttacaataga cgaaaacaaa gtagcaaaag
4801 aacataaaag acacttcaaa gaaagaaata cgatttgac
//