GenBank-Updates@genbank.bio.net (05/22/91)
LOCUS AFAACOXABC 5279 bp ds-DNA BCT 22-MAY-1991
DEFINITION A.eutrophus protein X (acoX), acetoin:DCPIP oxidoreductase-alpha
(acoA), acetoin:DCPIP oxidoreductase-beta (acoB), FMP (acoC), and
ORF 7 genes, complete cds, and ORF 8, 5' end.
ACCESSION M66060
KEYWORDS acetoin catabolism; acetoin dehydrogenase;
acetoin:DCPIP oxidoreductase; dihydrolipoamide acetyltransferase.
SOURCE A.eutrophus (strain H16) DNA.
ORGANISM Alcaligenes eutrophus
Prokaryota; Bacteria; Gracilicutes; Scotobacteria;
Aerobic rods and cocci; Alcaligenaceae.
REFERENCE 1 (bases 1 to 5279)
AUTHORS Priefert,H., Hein,S., Krueger,N., Zeh,K., Schmidt,B. and
Steinbuechel,A.
TITLE Identification and molecular characterization of the Alcaligenes
eutrophus H16 aco operon genes involved in acetoin catabolism
JOURNAL J. Bacteriol. (1991) In press
STANDARD full staff_entry
FEATURES Location/Qualifiers
promoter 53..69
/note="-24/-12 promoter"
mRNA 82..5279
CDS 108..1187
/product="protein X"
/gene="acoX"
/evidence=EXPERIMENTAL
/codon_start=108
CDS 1259..2260
/product="acetoin:DCPIP oxidoreductase-alpha"
/gene="acoA"
/evidence=EXPERIMENTAL
/codon_start=1259
CDS 2297..3313
/product="acetoin:DCPIP oxidoreductase-beta"
/gene="acoB"
/evidence=EXPERIMENTAL
/codon_start=2297
CDS 3313..4437
/note="probably a dihydrolipoamide acetyltransferase"
/product="FMP"
/gene="acoC"
/evidence=EXPERIMENTAL
/codon_start=3313
CDS 4517..4858
/note="ORF 7"
/evidence=EXPERIMENTAL
/codon_start=4517
CDS 4842..5279
/note="ORF 8"
/evidence=EXPERIMENTAL
/codon_start=4842
/partial
BASE COUNT 895 a 1765 c 1813 g 806 t
ORIGIN
1 ggatccgacc ggctgatacc cctgtttccg ctgttttcac tgcctgcgcg cactggcaca
61 gccattgcaa atacgcctgg caagacaacg acaacaggag acaggcaatg gggcacgcag
121 caggcgccag cgcgcagatc gcgccagtgg tcggcatcat cgccaacccg atttccgcgc
181 gcgacatccg tcgtgtgatc gccaacgcca acagcctgca gctggccgac cgcgtcaata
241 tcgtgctgcg cctgctggcc gcgctggcct cctgcggcgt ggagcgcgtg ctgatgatgc
301 ccgaccgcga aggcctgcgc gtcatgctgg cgcggcacct ggcacgccgg caggggccgg
361 actcgggcct gcccgcggtc gactacctcg acatgccggt caccgcgcgc gtcgacgaca
421 ccctgcgcgc cgcgcgctgc atggccgatg cgggcgtggc cgcgatcatc gtgctgggcg
481 gcgacggcac ccaccgcgcg gtggtgcgcg agtgcggcgc ggtgccgatc gcgggcctgt
541 cgaccggcac caacaatgcc taccccgaaa tgcgcgagcc caccatcatc gggctggcaa
601 ccggcgtcta tgccacgggc cgcattcctc ctgcgcaggc gctggcgtcg aacaagcggc
661 tcgacatcgt catccgtgac ggcaacggcg gctttcgccg cgatattgcg ctggtggatg
721 cggtgatctc gcacgagcac ttcatcggcg cgcgcgccct gtggaagacc gacacgcttg
781 ccgccgtcta cgtgtccttt gccgaccccg aggccatcgg cctgtcatcc atcgccgggc
841 tgctggagcc ggtgggccgg cgtgaggagg gcggcctcgc gatcgaactg gccgcgcccg
901 gcgaaggcga attcgacctg tgcgcgccga tcgcacccgg cctgatgtgc accgtgccgg
961 tggccggctg gcagcggctc gaacacggcc ggccccatcg cgtgcgccag cgcagcggca
1021 ttgtcgcgct ggacggagag cgcgagctgg ccttcggccc ggacgacgaa gtcacggtca
1081 ccctgcatga ccacgccttt cgcagcatcg acgtcgcggc ctgcatgcgc catgccgggc
1141 gccaccacct gatgcgcagc ctgccgcagc ccgcggcggt gggatgacgc gcgggcaccg
1201 cgtccgcccg cttttcccct ttcttgaagt gcaacacctg aaaacgaagg agacagacat
1261 gacagccaga gcttctcaag attccgccgc gctgccgctc gacaaggaga cgttgctgac
1321 ggtgtaccgg aagatgcgca ccatccgcga tttcgaagaa cgcctgcacg tggacttcgg
1381 gcgcggcgac atcccgggct tcgtccatct atacgcgggc gaggaagccg cgggcgtcgg
1441 cattctgcac cacctgaacg atggcgaccg catcgccagc acacaccgcg gccacggcca
1501 ttgcattgcc aagggcgtcg atccggtggc gatgatgaag gagatctacg gcaagaaggg
1561 cggctcgtgc aatggcaagg gtggctcgat gcatatcgcc gacctgtcca agggcatgat
1621 gggcgccaac ggcatcctgg gcgcaggcgc gccgctgatc tgcggcgccg cgctggctgc
1681 caagttccgc ggcaagggcg aggtcggcat caccttctgc ggcgacggcg cctccaacca
1741 gggcactttc ctggaaagcc tgaacctggc cgcggtctgg aacctgccgg tgatcttcgt
1801 gatcgagaac aacggctatg ccgaatcgac ctcgcgcgat tacggcaccg cggtggacag
1861 ctacgtggac cgcgccgccg gcttcggcat ccccggcgtg acggtggacg gcaccgattt
1921 cttcgccgtc catgaagcgg ccggcgaagt gatccggcgc gcgcgcgaag gcggcgggcc
1981 gtcgctgctc gaatgcaaga tggtccgctt ctatggccac ttcgagggcg atgcgcagac
2041 ctaccgcgcc gccggcgaac tcgacgacat ccgcgccaac aaggattgcc tgaagctgtt
2101 tggccgcgcc gtgacccagg cgggagtggt cgctcgcgaa gaactcgaca ccatcgaccg
2161 cgaggtcgcc gcgctgatcg agcatgccgt gcaggaagcc aaggccgcgc cgcagccggg
2221 tcccgaagac ctgctcaccg acgtctacgt cagctactga tctgccacgc gctaacccaa
2281 tatcaggaga caaaccatgg ctcgcaaact gagcatcaag ctggcgatca acgaggcgat
2341 cgaccaggaa atgacccgcg accccagcgt catcatgctg ggcgaagata tcgtcggcgg
2401 cgccggcgcg gacggcgaga aggacgcctg gggcggcgtg ctgggcgtga ccaagggtct
2461 ttacgccaag cacggcgacc ggctgctgga cacgccgctg tctgaatccg cttacgtggg
2521 cgccgccatc ggcgccgcgg cctgcggcat gcgcccgatc gccgagctga tgtttatcga
2581 tttcatgggc gtttgcttcg accagatctt caaccaggcg gccaagttcc gctacatgtt
2641 cggcggcaag gcggagacgc cggtggtgat ccgcgcgatg gtcggcgcgg gctttcgcgc
2701 agccgcgcag cacagccaga tgctgacgcc gctgttcacg catatccccg gcctgaaggt
2761 ggtgtgcccg tccacgccgt acgacaccaa gggcctgctg atccaggcga tccgcgacaa
2821 cgacccggtg atcttctgcg agcacaagaa cctgtacggc ctggaaggcg aggtgcccga
2881 aggcgcctac gcgatcccgt tcggcgaggc caatatcgtg cgcgacggca aggacgtgtc
2941 gatcgtcacc tacgggttga tggtgcatcg cgcgctcgaa gcggcggcaa cgctggccaa
3001 ggagggcatc gaggccgaga tcgtggacct gcgcacgctc tcgccactgg acatggacac
3061 ggtgctggag tcggtcgaga acaccggccg cctggtggtg gtggacgagg ccagcccgcg
3121 ctgcaatatc gccaccgata tctccgcgca ggttgcgcag caagccttcg gcgcgctcaa
3181 ggccggcatc gagatggtgt gcccgccgca cacgccggtg ccgttctcgc cgacgctgga
3241 ggacctgtac atccccagcg ccgcgcagat cgcggccgcc gcgcgcaaga ccatgaaggg
3301 aggcaagcac tgatggcaac cgaaatttcc cccacgatta tcccgatcgt gatgcccaag
3361 tggggcctgt cgatgaagga aggcacggtc aatgcgtggc tggtcgacga aggcaccgag
3421 attaccgtgg gcctgccgat cctggatgtg gaaaccgaca agatcgccaa tgcggtggag
3481 gcgcccgacg ccggcacgct gcgccgcaag gtggcgcagg ccggcgacgt gctgccggtg
3541 aaggcgctgc tgggcgtgct ggcacctgcg gaggtgagcg atgcgcaaat cgatggctat
3601 gtcgcggctt atgaaacgcc ggcggacgac gctggcgaag aggacgccgc tgccgcgtac
3661 cagtttgccg acggcgacgg catccgggtc cgctatgccc gcaagggtgg cggcgccgaa
3721 accgtgctct tcatccacgg atttggcgga gacctggaca actggctgtt caacctcgat
3781 cccctggccg acgcgtacac cgtggtggcg ctcgacctgc ccggccacgg acagtcgtcg
3841 ccgcggctcg cgggcacgac gctggcgcag atggccggct ttgtagcgcg cttcatggac
3901 gagaccggga tcgaggcggc gcatgtggtc ggccattcga tgggcggcgg cgtggcggcg
3961 caactggccg tggatgcacc gcagcgggtg ctgtcggtgg cgctggtgtc gccggtgggc
4021 tttggcgacg ccgtcaacag cggctatacc gaaggcttcg tcagtgccca gtcccggcgc
4081 gagctcaagc ccgtggtcga actgctgttc gccgacgcgg ggctggtcag ccgccagatg
4141 ctggacgacc tgctgcgcta caagcggctg gacggcgtca ccgaggcgct gacggcgctg
4201 gggcagggac tcttcggcgg cggccgccag agcgagcagc ctggccagcg gctggccaac
4261 agcggcaagc gcgtgctggt ggtgtggggc ggtcaggacc agatcatccc ggccgcgcac
4321 gctgaagccg cgccgccggg agcaaccgtc aaggtctttg ccgatgctgg ccacatgagc
4381 cagatggaga aggccaatga cttcaacgcg ctgctgaaga agcatctggg cggctgatgc
4441 cgcgcgcgga tggcgccgcc cagggcgcca tccgctcggc cagctcgcct tgttccaatt
4501 caagacagga gacctcatga ccacagacct gaagacccgc ctgaccgaga tcaacaggca
4561 attcggtgcg ctcggccagg cccagcccgc cgccatgagc gccttccagg gcgtgatgaa
4621 ggccgccacg caggacggca gcctgcccgc cgccgtcaag gaactgattg ccgtcgcgct
4681 ggcggtgcag aagggctgcg acgactgcgt gctgttccac accagccagg cgctgcgcca
4741 tcgcgccacg cgcgagcagc tcgccgaagt gctggccatc aatatcgaga tgggtggcgg
4801 acccggcgcc atgtacgcgt cgaaggcgct ggcgtacttc gatgcgctca atgcctagtg
4861 ttgctagact cggcacgttg ctatccggag gttggtgcca tcccatgccg tttgaattta
4921 ttgatgcggc ccatgccggg caggcgggca gcgacccgct gcaggatgag ctggccttgc
4981 tcgacctcgc ggcacaaggc cggcaggtgg cgcagctgtg ggaggcgccg ctgtcgctgg
5041 tgggcgcgcg cacctacctg cgccatgccg cgctggaaac cgcgcgcgcc gacttcgcgc
5101 agcagggatg tccggtattc ctgcgcatgt ccggcggcgg gctggtgccg cagggccccg
5161 gcatcctcaa cctgagcctg gcctacacag tggaacagcc gccgggcgcg cgcagcgacg
5221 cggtctacct gcacttgtgc gaggtcatcg gcgacgcgct gcaatcgctg ggcgtcgac
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