GenBank-Updates@genbank.bio.net (05/22/91)
LOCUS BACPDMC 6822 bp ds-DNA BCT 22-MAY-1991
DEFINITION B. stearothermophilus pdhA, pdhB, pdhC, pdhD genes for pyruvate
dehydrogenase multienzyme complex (E.C. numbers 1.2.4.1, 2.3.1.12,
1.8.1.4).
ACCESSION X53560
KEYWORDS dihydrolipoamide acetyltransferase; dihydrolipoamide dehydrogenase;
pyruvate dehydrogenase; pyruvate dehydrogenase E1-beta subunit;
pyruvate dehydrogenase alpha subunit.
SOURCE Bacillus stearothermophilus DNA.
ORGANISM Bacillus stearothermophilus
Prokaryota; Bacteria; Firmicutes; Endospore-forming rods and cocci;
Bacillaceae.
REFERENCE 1 (bases 1 to 6822; 4182 to 6822)
AUTHORS Hawkins,C.F.
JOURNAL Unpublished (1990)
STANDARD full automatic
REFERENCE 2 (bases 1 to 4181)
AUTHORS Borges,A., Hawkins,C.F. and Perham,R.N.
TITLE Cloning and Sequence Analysis of the Genes Encoding the Alpha- and
Beta- Subunit of E1 Component of the Pyruvate Dehydrogenase
Multienzyme Complex of Bacillus Stearothermophilus
JOURNAL Eur. J. Biochem. 194, 95-102 (1990)
STANDARD full automatic
COMMENT *source: strain=NCA 1503; library=genomic (plasmid); *source:
clone= pBst42 & pBst27;
From EMBL 26 entry BSPDMC; dated 11-MAR-1991.
FEATURES Location/Qualifiers
prim_transcript <1430..>2539
/note="E1 alpha subunit"
/gene="pdhA"
prim_transcript <2543..>3520
/note="E1 beta subunit"
/gene="pdhB"
prim_transcript <3595..>4880
/note="E2"
/gene="pdhC"
prim_transcript <4885..>6297
/note="E3"
/gene="pdhD"
CDS 1..288
/note="ORF 1"
/codon_start=1
terminator 287..328
/note="rho-independant terminator"
RBS 1262..1272
CDS 1290..1367
/note="ORF 2"
/codon_start=1290
RBS 1418..1424
mat_peptide 1430..2536
/note="E1 alpha subunit"
/product="pyruvate dehydrogenase (lipoamide)"
/gene="pdhA"
CDS 1430..2539
/note="E1 alpha subunit"
/product="pyruvate dehydrogenase (lipoamide)"
/gene="pdhA"
/codon_start=1430
RBS 2525..2534
mat_peptide 2543..3517
/note="E1 beta subunit"
/product="pyruvate dehydrogenase (lipoamide)"
/gene="pdhB"
CDS 2543..3520
/note="E1 beta subunit"
/product="pyruvate dehydrogenase (lipoamide)"
/gene="pdhB"
/codon_start=2543
RBS 3579..3583
mat_peptide 3594..4877
/note="E2"
/product="dihydrolipoamide acetyltransferase"
/gene="pdhC"
CDS 3594..4880
/note="E2"
/product="dihydrolipoamide acetyltransferase"
/gene="pdhC"
/codon_start=3594
RBS 4871..4875
mat_peptide 4885..6294
/note="E3"
/product="dihydrolipoamide dehydrogenase"
/gene="pdhD"
CDS 4885..6297
/note="E3"
/product="dihydrolipoamide dehydrogenase"
/gene="pdhD"
/codon_start=4885
BASE COUNT 1716 a 1655 c 1981 g 1470 t
ORIGIN
1 gaattccgtt ttgtccgttg gcacgatgtg tcgaccgatg tgctgccggc agggggatcg
61 aaagcggagg ggatccgcct gatgattgag aagctcggga ttgacaaagg cgatgtgtat
121 gcgttcggcg atgggctgaa cgacattgaa atgttgtcgt tcgtcggaac aggagtggcg
181 atgggaaatg cccatgagga agtgaaacgg gtcgccgatt ttgtaacgaa accggtcagt
241 gaagaaggca tatggcacgg attgaaacaa ttgcaattga ttccataaac agctgccccg
301 ggccgctcat tcggcggggc agctgtttgg catggccggc cacggttcag cgcccgatcg
361 ggatcgcccc gtctggcact tggaacgggt cggccggatt gatgcggtca taaaacataa
421 tgccgtttaa atggtcgatt tcatgctgga agacgatggc cggcaatcct ttgaggcgca
481 aggtgacttc ctcgccgtcg agcgtcgtgc cggtgaccgt gatgcgggca tagcgcagta
541 cgtatccggg cacatcacgg tcgaccgaca ggcagccttc ccccgtcgtc aaataacatt
601 gctgcacaga gtggctgacg attttcggat tgaacaaggc atagctgtac agcgttccgt
661 tttcatcagt gacatgaacg gcaatcatcc gtttggagac gttgatttgc ggagcggcga
721 ggccgatgcc ggccgcaggc cgtatttggc cgccagttcc ggatcttggc tcattttcac
781 atagtcaagc aagctttgca aaatgcgttt gtcttcctct gaaggcggca gcggaacggg
841 ctcagccact ttccgcagcg tcggatgccc ttctttaatg atatctttca tggtgatcat
901 tcgccttttc actcctgtct ctcgcatcgc ggtctacagt gttagtctaa caaaaatgcc
961 tgtgcgccgt aaatgaaaat gcggccgtct ggagatatta tcggcgtata aggaataaat
1021 tggaaaataa acttaagaaa gcgctgtcaa taacatgaac ggacattgcg ttttgcattt
1081 cccgaaaaat ataatacaga attaatacag tgttgtttcg atgtggtata acagatggtt
1141 tcaggaatgt tcctgattcc aaatacaacc aggagacaaa agggaaagca acggaaaatc
1201 aaagtcgttg acggcgtgaa aaataaagtg taaactaaaa cttgagaaca gacgctgtgt
1261 taatgaggat ataaaaaata gtaatacaga tgtcggcgcc gttttcggtt tccgttgcgc
1321 caagcggcgg ctgcttttta tttcttcttt acttttgggt acattaatag tatatggatg
1381 aagacagcct gatttccaaa tttcttgatg aaaggcagag gtgaacaaga tgggtgtaaa
1441 gaccttccaa ttcccatttg cggagcagct cgagaaagtg gccgagcagt tcccgacatt
1501 tcaaattttg aatgaggaag gagaagtcgt caatgaagag gcgatgccgg agctgagcga
1561 tgagcagctg aaagagctga tgcgtcgcat ggtgtatacg cgcattcttg accagcgctc
1621 catttcgttg aaccgccaag ggagactcgg gttttatgcg ccgaccgccg ggcaggaagc
1681 gagccaaatt gccagccatt ttgcattgga aaaagaagat tttattttgc cgggataccg
1741 cgacgttccg caaatcattt ggcatgggct tccgctgtat caggcgtttt tgttctcgcg
1801 cggccatttc catggtaacc aaattccaga aggcgtaaac gtcctgccgc cgcaaatcat
1861 tatcggcgcc caatacatcc aagcagctgg tgtggcgctc ggcttgaaaa tgcggggcaa
1921 aaaagcagtg gcgattacat atacgggcga cggcggcacg tcgcaaggcg atttctacga
1981 agggatcaac tttgcggggg cgtttaaggc gccggccatc tttgtcgtgc aaaacaaccg
2041 ctttgccatc tcgacgccgg tcgaaaagca aaccgttgcc aaaacattgg cgcaaaaagc
2101 agtcgccgcc ggcattccgg gcattcaagt tgacggcatg gacccgcttg cggtctacgc
2161 agcggtcaaa gctgctcgcg agcgcgccat taacggcgaa gggccgacgc tcattgaaac
2221 gctctgcttc cgctatggcc cgcatacgat gtccggtgac gatccaacgc gctatcgttc
2281 gaaagagttg gaaaacgaat gggcgaagaa agacccactc gtgcgtttcc gcaagttttt
2341 agaggcgaaa ggcttgtgga gcgaagaaga agaaaacaac gttattgaac aggcaaaaga
2401 agagattaaa gaagcgatca agaaagcgga cgaaacgccg aagcaaaaag taaccgactt
2461 gatcagcatc atgtttgaag agctgccgtt caatttgaag gagcagtatg aaatttacaa
2521 agagaaggag tcgaagtaag ccatggcgca aatgacaatg gttcaggcga tcaccgatgc
2581 gctgcgtatc gagttgaaaa atgatccgaa cgtgctcatt ttcggggaag acgttggggt
2641 caacggcggc gtattccggg cgacggaagg gctgcaggcc gaatttggcg aagatcgcgt
2701 ttttgacacg ccgctcgccg aatcaggaat cggcggcttg gcgatcggtt tggcgctgca
2761 agggtttcgc ccggtgccgg aaatccagtt tttcggcttc gtgtatgaag tgatggattc
2821 gatttgcggg caaatggcgc gaatccgcta tcgtacgggc ggacgctacc atatgccgat
2881 tacgatccgt tctccgtttg gcggcggcgt ccatacgccg gagttgcact cagacagctt
2941 ggaagggctt gtcgcccaac agccaggctt gaaggttgtc attccgtcga cgccgtatga
3001 tgcgaaaggg ctgctcatct cggcgattcg cgacaacgac ccggtcattt tccttgaaca
3061 tttgaagctg taccgttcgt tccgtcagga agtgccggaa ggggaataca cgattccgat
3121 cggcaaggcg gacattaagc gggaaggaaa agacatcacg atcatcgcgt acggcgccat
3181 ggtgcatgaa tcgttaaaag cggcggcaga attagagaag gaaggcattt ctgccgaagt
3241 cgtcgacttg cgtaccgtgc agccgctcga tattgaaacg atcatcggtt cggtcgaaaa
3301 aacgggccgc gccattgtcg ttcaagaagc gcaacggcag gctggcatcg ccgccaacgt
3361 cgtggcggag attaacgaac gcgccatctt aagccttgag gcgccggtgt tgcgcgtggc
3421 cgctccggac acggtctatc cgtttgctca ggcggaatcg gtatggctgc caaactttaa
3481 agatgtaatc gaaacggcga aaaaagtgat gaacttctaa cagcggacaa attcggggga
3541 gcggaacgcc ttcccccgcc cattttcggc aaacgatagg aggtagacag acagtggctt
3601 ttgaatttaa gctgccggac attggcgaag gcatccacga aggtgaaatt gtcaaatggt
3661 ttgtgaaacc gggcgatgaa gtgaacgaag acgatgtatt gtgcgaagtg caaaatgaca
3721 aggcggttgt cgaaattccc tccccggtca aagggaaagt gcttgaaatc ctcgtcccgg
3781 agggaacagt ggcaacggtc gggcaaacgc tcatcacgct cgatgcgccg ggttatgaaa
3841 acatgacgtt taaaggacaa gagcaagaag aagcgaaaaa agaggaaaaa acggaaacgg
3901 tgtcgaaaga ggaaaaggtt gacgctgtcg ctcccaatgc accggcagct gaagcggagg
3961 ctggcccgaa ccgccgcgtc atcgccatgc cgtccgtgcg caagtatgcg cgcgaaaaag
4021 gcgtcgatat tcggcttgtc caaggaacgg gcaaaaacgg ccgcgtcctg aaagaagata
4081 ttgacgcttt ccttgccggc ggcgcaaaac cggcaccggc cgcagcggag gaaaaagcgg
4141 cgccggccgc agcgaaaccg gcgacgacag aaggcgaatt cccggaaacg cgcgagaaaa
4201 tgagcggcat ccgtcgggcg attgccaaag cgatggtgca ttccaaacat accgctccgc
4261 acgtgacgct gatggacgaa gccgatgtga cgaagcttgt tgctcaccga aaaaaattca
4321 aggctattgc cgcggaaaaa ggcatcaagc tgacgttttt gccgtacgtc gtcaaagcgc
4381 tcgtttccgc gctgcgtgaa tacccagtgt tgaatacgtc cattgatgat gagacggaag
4441 aaatcatcca gaagcattat tacaatatcg gcatcgccgc tgatacggac cgcggcttgc
4501 ttgtgccggt cattaaacat gccgatcgca agccgatttt cgccttggcg caagaaatca
4561 atgagctcgc cgagaaagcg cgcgacggca aactgacgcc gggagaaatg aaaggcgctt
4621 catgcacgat tacaaacatc ggttcggctg gcgggcaatg gtttaccccg gtcatcaacc
4681 atccggaagt ggcgattctt ggcatcggcc gcattgcgga aaagccgatc gtccgtgacg
4741 gtgaaattgt cgctgccccg atgctggcgc tctcgctcag ctttgaccac cggatgatcg
4801 acggggcgac agcgcaaaaa gcgctcaacc atatcaaacg gctgttgagt gacccggaat
4861 tattactaat ggaggcgtaa aacgatggta gttggcgatt ttgcaattga aacggaaacg
4921 cttgtcgtcg gcgccggtcc tggcgggtat gtcgccgcca tccgcgccgc gcagctcgga
4981 caaaaagtga cgattgtgga aaagggcaat ttaggcggcg tctgcttaaa tgttggctgc
5041 atcccgtcaa aggcgctgat ctccgcaagc catcgctatg agcaggcgaa gcattccgaa
5101 gaaatgggca ttaaggcgga gaacgtcacc atcgattttg ccaaagtgca agaatggaaa
5161 gcaagcgtcg tgaaaaaatt aacgggcggc gtcgaagggc tgttaaaagg aaacaaagta
5221 gagatcgtca aaggggaagc gtattttgtc gacgccaata cggtgcgtgt cgtcaacggc
5281 gacagcgcgc agacgtatac gttcaaaaac gcgattatcg ccaccggctc gcgcccgatt
5341 gagctgccga acttcaagtt ttccaaccgc attcttgact cgacgggagc tctcaacctt
5401 ggggaagtgc cgaaatcgct cgtggtcatc ggcggcggct atattggcat cgaactcggt
5461 acggcttacg ccaactttgg gacgaaagtg accattttag aaggagccgg cgaaattttg
5521 tccggttttg agaagcaaat ggctgccatc atcaagaagc gcctgaagaa aaaaggcgtt
5581 gaagtcgtga cgaacgcatt ggcgaaaggg gccgaagaac gcgaagacgg cgtcacggtc
5641 acgtatgagg cgaatggtga aacgaaaacg attgacgccg actatgtgct cgtcaccgtc
5701 ggccgccgtc cgaatacaga tgaacttggt cttgaacaaa tcggcatcaa aatgacgaac
5761 cgcggcttga ttgaagtgga ccaacaatgc cggacaagcg tgccgaacat ttttgcgatc
5821 ggcgacatcg ttccaggtcc ggcgctcgcc cataaagcgt cgtatgaagg aaaagtggct
5881 gcggaagcta tcgccggcca tccgtcggca gtcgattacg tggccattcc agctgttgtc
5941 ttctccgatc cggaatgcgc gtcggtcggc tattttgagc agcaggcgaa agacgagggc
6001 attgacgtca ttgccgcgaa attcccgttt gctgccaacg gccgcgcctt ggcgctgaac
6061 gatacggacg gcttcttgaa gctcgttgtc cgcaaagagg acggcgtcat cattggtgcg
6121 caaatcatcg gtccaaatgc ttctgacatg atcgccgagc ttgggctcgc cattgaagcc
6181 ggcatgacgg cggaagacat cgctttgacg atccatgccc atccgacgct tggcgaaatc
6241 gccatggaag cggcggaagt ggcgcttggc acaccgattc atatcattac gaagtaactg
6301 tgaaacgacc gggcttggcc cggtcgtttt tcttatcatg cgggaatggc gcatttcttg
6361 tcctgttttt catactattt tccgctttcg tttcatatcg ttatggtaaa gaaaagcggg
6421 agatggaaat gatgaaagcg tatgtcgcct ttttgctgca aatgatgatt tggagcagtt
6481 tttccttgat cgagtggctg tcggggcggg accggccgtt tttccgcggc ttgatgttgt
6541 gcgtcttcat gtacatagcg tttttactgg cgcgccagct cggcttggcg acgcgcaaag
6601 cgctccttac aacagctgcc acggcggcgg tgtattttac cggccagcat ttcatatggc
6661 aatctattcc atgatctgcc ccttgggtgc ggaagcggcg cggttcggcg aacaaaaaac
6721 agcggtccca tgccgctgtt tacaccggcc gaacgacatc gtccggccgc tgaataaacg
6781 cagttcgccg ccaagcgttt tggtcatcca tttgctgaat tc
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