GenBank-Updates@genbank.bio.net (05/22/91)
LOCUS SHPPPCHY 1292 bp ss-mRNA MAM 22-MAY-1991
DEFINITION Lamb mRNA for preprochymosin (EC 3.4.23.4)
ACCESSION X53037
KEYWORDS chymosin; preprochymosin; secretory protein.
SOURCE Ovis aries RNA.
ORGANISM Ovis aries
Eukaryota; Animalia; Metazoa; Chordata; Vertebrata; Mammalia;
Theria; Eutheria; Artiodactyla; Ruminantia; Pecora; Bovidae.
REFERENCE 1 (bases 1 to 1292)
AUTHORS Gubensek,F.
JOURNAL Unpublished (1990)
STANDARD full automatic
REFERENCE 2 (bases 1 to 1292)
AUTHORS Pungecar,J., Strukelj,B., Gubensek,F., Turk,V. and Kregar,I.
TITLE Complete primary structure of lamb preprochymosin deduced from cDNA
JOURNAL Nucleic Acids Res. 18, 4602-4602 (1990)
STANDARD full automatic
COMMENT *source: clone=LC 13-9; library=pUC19; *source: developmental
stage=suckling animal;
From EMBL 26 entry OAPPCHY; dated 11-SEP-1990.
FEATURES Location/Qualifiers
CDS 13..1155
/note="preprochymosin (AA -16 to 365)"
/codon_start=13
CDS 13..60
/note="signal peptide (AA -16 to -1)"
/codon_start=13
CDS 61..186
/note="prochymosin (AA 1-42)"
/codon_start=61
CDS 187..1155
/note="mature chymosin (AA 43-365)"
/codon_start=187
misc_feature 1273..1278
/note="polyA signal"
BASE COUNT 285 a 385 c 353 g 269 t
ORIGIN
1 cccagatcca agatgaggtg tcttgtggtg ctacttgctg tctttgctct ctcccagggc
61 gctgagatca ccaggatccc actgtacaaa ggcaagcctc tgaggaaggc actgaaggag
121 cgtgggcttc tggaggactt cctgcagaaa cagcaatatg gcgtcagcag cgagtactcc
181 ggctttgggg aggtggccag tgtgcccttg accaattacc tggatagtca gtactttggg
241 aagatctacc tcgggacccc gccccaggag ttcaccgtgc tgtttgacac cggctcctct
301 gacttctggg taccctctat ctactgcaag agcaatgcct gcaaaaacca ccagcgcttc
361 gacccaagaa agtcgtccac cttccagaac ctgggcaagc ccctgtctat ccgctatggg
421 acgggcagca tgcagggcat cctgggctac gacaccgtca ctgtctccaa cattgtggac
481 atccagcaga cagtaggcct gagcacccag gagcctgggg atgtcttcac ctatgccgag
541 ttcgacggga tcctggggat ggcctacccc tcgctcgcct cagagtactc ggtgcccgtg
601 tttgacaaca tgatggacag gcgcctggtg gcccaggacc tgttctcggt ttacatggac
661 aggagtggcc aggggagcat gctcacactg ggggccatcg acccgtccta ctacacaggg
721 tccctccact gggtgcccgt gacgctgcag aagtactggc agttcaccgt ggacagtgtc
781 accatcagcg gtgcggttgt ggcctgtgag ggtggctgtc aggccatcct ggacacgggc
841 acctccaagc tggtcgggcc cagcagcgac atcctcaaca tccagcaggc cattggagcc
901 acacagaacc agtatggcga gtttgacatc gactgcgaca gcttgagcag catgcccact
961 gtggtctttg agatcaatgg caaaatgtac ccactgaccc cctacgccta taccagccag
1021 gaggagggct tctgcaccag tggcttccag ggtgaaaatc attcccatca atggatcctg
1081 ggggatgttt tcatccgaga gtattacagc gtctttgaca gggccaacaa cctcgtgggg
1141 ctggccaaag ccatctgatc acatcgctga ccaagaacct cactgtcccc acacacctgc
1201 acatacacac gcacacgtgt acgtgagcac acgtgtgcac acacagatga ggtttccaga
1261 cagatgattc tcaataaatg ttgtctttct gc
//