GenBank-Updates@genbank.bio.net (05/22/91)
LOCUS SHPTNFA 1499 bp ss-mRNA MAM 22-MAY-1991
DEFINITION Ovine TNF-alpha gene for tumor necrosis factor alpha
ACCESSION X55966
KEYWORDS cytokine; tumor necrosis factor alpha.
SOURCE Ovis aries RNA.
ORGANISM Ovis aries
Eukaryota; Animalia; Metazoa; Chordata; Vertebrata; Mammalia;
Theria; Eutheria; Artiodactyla; Ruminantia; Pecora; Bovidae.
REFERENCE 1 (bases 1 to 1499)
AUTHORS Young,A.J.
JOURNAL Unpublished (1990)
STANDARD full automatic
REFERENCE 2 (sites)
AUTHORS Young,A.J., Hay,J.B. and Chan,J.Y.
TITLE Primary structure of ovine tumor necrosis factor alpha cDNA
JOURNAL Nucleic Acids Res. 18, 6723-6723 (1990)
STANDARD full staff_review
COMMENT *source: library=lambda gt11; tissue=liver; clone=sTNF-2.
From EMBL 26 entry OATNFA; dated 15-NOV-1990.
FEATURES Location/Qualifiers
mRNA <4..>1488
mat_peptide 4..702
/product="tumor necrosis factor alpha"
/gene="sTNF-alpha"
CDS 4..705
/product="tumor necrosis factor alpha"
/gene="sTNF-alpha"
/codon_start=4
misc_feature 1163..1196
/note="polyAT regulatory region"
polyA_signal 1483..1488
BASE COUNT 331 a 441 c 369 g 358 t
ORIGIN
1 cggatgagca ccaaaagcat gatccgggat gtggagctgg cggaggaggt gctctccaac
61 aaagcagggg gcccccaggg ctccagaagt tgctggtgcc tcagcctctt ctccttcctc
121 ctggttgcag gagccaccac gctcttctgc ctgctgcact tcggggtaat cggcccccag
181 agggaagagt ccccagctgg cccctccttc aacaggcctc tggttcagac actcaggtca
241 tcttctcaag cctcaaataa caagccggta gcccacgttg tagccaacat cagcgctccg
301 gggcagctcc gatgggggga ctcgtatgcc aatgccctca tggccaacgg cgtggagctg
361 aaagacaacc agctggtggt gcccactgac gggctttacc tcatctactc acaggtcctc
421 ttcaggggcc acggctgccc ttccaccccc ttgttcctca cccacaccat cagccgcatt
481 gcagtctcct accagaccaa ggtcaacatc ctctctgcca tcaagagccc ttgccacagg
541 gagaccctag agggggctga ggccaagccc tggtacgaac ccatctacca gggaggggtc
601 ttccagctgg agaagggaga tcgcctcagt gctgagatca acctgccgga atacctggac
661 tatgccgagt ctgggcaggt ctactttggg atcatcgccc tgtgaggcgc aggacatgca
721 tcctctccca cctcagttac cttattattt actccttcag accctcctca tccccttctg
781 gtttagaaag ggaattaggg gctcagggct gggctccaag cgtccaactt taaacaacag
841 ctgcacttag aaattaggga tgtagggaag tgaggcctgg acaacgggcc accaaccatc
901 accaaggact ggaactggaa cttccagaac tcctcgggtc cacaagtttg ggttcccgga
961 tgcaacctgg gacacccaga atgcaagggc cagggttctt accggaatac ttcgcaacgt
1021 tccttgagaa gatctcacct agaacttgac atgggtgggc ttcaactctc ccttcctgcc
1081 aatgtttcca gattcccctg aggtgggagc cccagcccca accccactgg gccaactccc
1141 tctgtttatg tttgcactta tgattattta ttatttattt attatttatt tatttactaa
1201 tgaatgtatt tattcaggag gtcaaggtgt cctgggagac acaaactaag ggctgccttg
1261 gctcagatgt gttttctgtg aaaacggagc tgaactgcag gttctcccac catgcctcct
1321 gcctttgtgc ctccttttgc ttatgttttt taaaaaataa ttatgtgcat caagttgtct
1381 aaatgatgcc tgatttggtg actgatttgt cgctacactc actgcaacct ccgctcccca
1441 ggggagtcat gcctgtaacc gccctactgg tcagtggcga gaaataaagt gtcctgccg
//