GenBank-Updates@genbank.bio.net (05/22/91)
LOCUS VFAVFRN1 1409 bp ds-DNA ORG 22-MAY-1991
DEFINITION Broad bean chloroplast genes for tRNA-Leu(CAA) and (UAA) and
tRNA-Phe
ACCESSION X02444 M23771 X00443
KEYWORDS direct repeat; tandem repeat; transfer rna; transfer rna-leu;
transfer rna-phe.
SOURCE Chloroplast Vicia faba DNA.
ORGANISM Chloroplast Vicia faba
Eukaryota; Plantae; Embryobionta; Magnoliophyta; Magnoliopsida;
Rosidae; Rosales; Fabaceaea.
REFERENCE 1 (sites)
AUTHORS Bonnard,G., Michel,F., Weil,J.H. and Steinmetz,A.
TITLE Nucleotide sequence of the split tRNA Leu UAA gene from Vicia faba
chloroplasts: evidence for structural homologies of the chloroplast
tRNA Leu intron with the intron from the autosplicable Tetrahymena
ribosomal RNA precursor
JOURNAL Mol. Gen. Genet. 194, 330-336 (1984)
STANDARD full staff_review
REFERENCE 2 (bases 1 to 1409)
AUTHORS Bonnard,G., Weil,J.H. and Steinmetz,A.
TITLE The intergenic region between the vicia faba chloroplast
tRNA-Leu(CAA) and tRNA-Leu(UAA) genes contains a partial copy of
the split tRNA-Leu(UAA) gene
JOURNAL Curr. Genet. 9, 417-422 (1985)
STANDARD full automatic
REFERENCE 3 (bases 636 to 638)
AUTHORS Steinmetz,A.
JOURNAL Unpublished (1986)
STANDARD full automatic
COMMENT Data kindly reviewed (02-APR-1986) by A. Steinmetz
From EMBL 26 entry CHVFRN1; dated 13-MAR-1991.
FEATURES Location/Qualifiers
tRNA complement(15..95)
/note="tRNA-Leu (CAA)"
intron 96..538
/note="intron"
promoter complement(125..130)
/note="put. -10 region"
promoter complement(147..152)
/note="put. -35 region"
repeat_region 164..170
/note="direct repeat a"
repeat_region 175..181
/note="direct repeat a"
repeat_region 187..205
/note="direct repeat b"
repeat_region 206..224
/note="direct repeat b"
repeat_region 225..233
/note="truncated direct repeat b"
repeat_region 236..415
/note="direct repeat c"
repeat_region 439..615
/note="direct repeat c"
tRNA 539..573
/note="tRNA-Leu (UAA) part 1"
intron 574..1024
/note="intron"
old_sequence 636..638
/note="TGG was TAG in [1]"
/citation=[2]
tRNA 1025..1074
/note="tRNA-Leu (UAA) part 1"
intron 1075..1184
/note="intron"
promoter 1128..1133
/note="put. -35 like region"
promoter 1153..1158
/note="put. -10 like region"
promoter 1159..1164
/note="put. -10 like region"
tRNA 1185..1257
/note="tRNA-Phe"
BASE COUNT 505 a 240 c 239 g 425 t
ORIGIN
1 agatctcaat attatgcctt gaagaggact cgaacctcca cgctgtttag cacgagattt
61 tgagtctcgc gtgtctacca tttcaccacc aaggcatcct taaagggaat catattctct
121 gaatatgata tctatcttat gtcatggttc aatattatta tactccaatt ctaatccaat
181 tgcaacatag aatacaacat ctcaaataga atacaacatc tcaaatagaa tacggtacga
241 tataatacag tatctactat ataatacagc gagaatagaa ttctttctcg actaaataaa
301 tatagcttct agatgaacga aaagctagtc aaagggtcga ttcaaaattg gtagacgcta
361 cggacttaat tgtattgagc tttggtatgg aaacatagta atgttaagtg aaaacataac
421 ataatataat acagcatcta ctatataata caacatgtac tatataatac agcgagaaga
481 gaattctttc tcgactaaat aaatatagct tctagatgaa cgaaaagcaa gtcaaacggg
541 ggatatggcg aaattggtag acgctacgga cttaattgta ttgagccttg gtatggaaac
601 atattaagtg aaaactttca aattcagaga aaccctggaa ttttaaatgg gcaatcctga
661 gccaaatcct tctttccgaa aacaaaaaac taaaagttca gaaaaaaagg ataggtgcag
721 agactcaatg gaagctattc taacaaatgg agttgacaac attcaattga ttaatgaaga
781 tttctaactt ctatttgtaa attgtaacta ttttttttag ttttgattcg atcacaattg
841 aaacgaaaca ttagaatcaa atcaattcca actggaagaa aaaatggtga atattcattg
901 atcaaatcag tcactccacc aaaatatgat ggatcttttg aataactgat taatcagacg
961 agaataaaga tagagtccca ttctacatgt caataccgac atcaatgaaa gttttagtaa
1021 tcggaaaatc cgtcgacttt aaaaatcgtg agggttcaag tccctctatc cccaaaagcc
1081 gattgaattt gctcattttt atggcccttt cccttttttt tattgagttg acatagactc
1141 aagtaatttc cctcaaatta ggatggtgcg tcaagatttt aatggtcggg atagctcagc
1201 cggtagagca aaggactgaa aatccttgtg tcaccagttc aaatctggtt cccggcgact
1261 cattatgtat gagtatctat tcccatattt cttttcaaaa atcaaatatt gggaggaata
1321 gatacatatt gattagtggt cttaataatt atacataatt tatctaggcg tacagagata
1381 tgctctttct agatgaaaaa ggaatcgat
//