GenBank-Updates@genbank.bio.net (05/22/91)
LOCUS ECOHYP 3793 bp ds-DNA BCT 22-MAY-1991
DEFINITION E. coli hyp operon encoding hydrogenase isoenzymes
ACCESSION X54543
KEYWORDS hydrogenase; hyp operon.
SOURCE Escherichia coli DNA.
ORGANISM Escherichia coli
Prokaryota; Bacteria; Gracilicutes; Scotobacteria;
Facultatively anaerobic rods; Enterobacteriaceae.
REFERENCE 1 (bases 1 to 3793)
AUTHORS Boehm,R.
JOURNAL Unpublished (1990)
STANDARD full automatic
REFERENCE 2 (bases 1 to 3793)
AUTHORS Lutz,S., Jacobi,A., Schlensog,V., Boehm,R., Sawers,G. and Boeck,A.
TITLE Molecular characterization of an operon(hyp) necessary for the
activity of the three hydrogenase isoenzymes in Escherichia coli
JOURNAL Mol. Microbiol. 5, 123-135 (1991)
STANDARD full automatic
COMMENT From EMBL 26 entry ECHYP; dated 13-MAR-1991.
FEATURES Location/Qualifiers
promoter 57..70
/note="hypABCD -24/-12 promoter"
/evidence=EXPERIMENTAL
prim_transcript 81..>3719
/gene="hypABCDE"
/evidence=EXPERIMENTAL
CDS 102..452
/product="hypA product"
/gene="hypA"
/codon_start=102
promoter 254..284
/note="Fnr-dependent promoter"
/evidence=EXPERIMENTAL
prim_transcript 293..>3719
/gene="hypBCDE"
/evidence=EXPERIMENTAL
CDS 456..1328
/product="hydrogenase isoenzyme hypB"
/gene="hypB"
/codon_start=456
CDS 1319..1591
/product="hydrogenase isoenzyme hypC"
/gene="hypC"
/codon_start=1319
CDS 1591..2712
/product="hydrogenase isoenzyme hypD"
/gene="hypD"
/codon_start=1591
CDS 2751..3719
/product="hypE product"
/gene="hypE"
/codon_start=2751
BASE COUNT 864 a 1003 c 1088 g 838 t
ORIGIN
1 gtcgacgtgt catttcgaca tcatcgacat tattcaccgc agggataatc aacactggca
61 caattattgc ttgtagctgg caatagttaa tgggaggcga tatgcacgaa ataaccctct
121 gccaacgggc actggaattg atcgaacagc aggccgcaaa acacggcgca aaacgcgtaa
181 ctggggtctg gctcaaaatt ggcgcatttt cttgtgtcga aaccagctct cttgcctttt
241 gttttgatct ggtttgccgc ggcagcgtgg cggaaggttg taaactgcac ctcgaagaac
301 aagaggccga atgctggtgt gaaacatgcc aacagtatgt gacgctactg acccagcgcg
361 tccgccgctg tccacagtgt catggtgaca tgctgcagat tgtggcagac gacggtttac
421 agattcggcg gatagaaata gaccaggagt gagcgatgtg tacaacatgc ggttgcggtg
481 aaggcaacct gtatatcgag ggtgatgaac ataaccctca ttccgcgttt cgtagcgcgc
541 catttgcccc ggcggcacgc cggaagatga aaatcaccgg cattaaagcg cctgaattta
601 cccccagcca gactgaagaa ggcgacctgc attacggtca tggcgaagcg ggcactcacg
661 caccgggcat gagccagcgt cggatgctgg aagtcgaaat tgacgtgctg gacaaaaata
721 accgtctggc tgaacgcaac cgcgcgcgct ttgctgcccg caagcaactg gtgctcaacc
781 tggtttccag ccctggttcc ggtaaaacca ccctgctgac ggaaacccta atgcgcctga
841 aagacagcgt tccgtgcgca gttattgaag gcgaccagca aaccgtgaac gatgccgcac
901 gcattcgcgc taccggcaca ccagcgattc aggtgaacac cggtaaaggc tgccatcttg
961 acgcacagat gattgccgac gccgcaccgc gtctgccact ggacgataac ggtattctgt
1021 ttatcgaaaa cgttggcaac ctcgtatgcc cggccagctt cgatctcggt gaaaaacaca
1081 aagtggcggt gctttccgtt accgaaggtg aagacaaacc actgaaatat ccgcatatgt
1141 ttgccgccgc ctcgctgatg ctgctcaaca aagttgacct gttgccgtat ctcaactttg
1201 acgttgaaaa gtgcatcgcc tgcgcccgcg aagtcaatcc agaaattgaa atcatcctta
1261 tttccgccac cagcggcgaa gggatggacc agtggctgaa ctggctggag acacagcgat
1321 gtgcataggc gttcccggcc agatccgcac cattgacggc aaccaggcga aagtcgacgt
1381 ctgcggcatt cagcgcgatg tcgatttaac gttagtcggc agctgcgatg aaaacggtca
1441 gccgcgcgtg ggccagtggg tactggtaca cgttggcttt gccatgagcg taattaatga
1501 agccgaagca cgcgacactc tcgacgcctt acaaaacatg tttgacgttg agccggatgt
1561 cggcgcgctg ttgtatggcg aggaaaaata atgcgttttg ttgatgaata tcgcgcgccg
1621 gaacaggtga tgcagttaat tgagcatctg cgcgaacgtg cttcacatct ctcttacacc
1681 gccgaacgcc ctctgcggat tatggaagtg tgtggcggtc atacccacgc tatctttaaa
1741 ttcggcctcg accagttact gccggaaaac gttgagttta tccacggtcc ggggtgcccg
1801 gtgtgcgtac tgccgatggg tagaatcgac acctgcgtgg agattgccag ccatccggaa
1861 gtcatcttct gtacctttgg cgacgcgatg cgcgtgccgg ggaaacaggg atcgctgttg
1921 caggcaaaag cacgcggtgc cgatgtgcgc atcgtttact cgccgatgga tgcgttgaaa
1981 ctggcgcagg agaatccaac ccgcaaagtg gtgttcttcg gcttaggttt tgaaaccact
2041 atgccgacca ccgctatcac tctgcaacag gcgaaagcgc gtgatgtgca gaatttttac
2101 ttcttctgcc agcacattac gcttatcccg acgttgcgca gtttgctgga acagccggat
2161 aacggtatcg atgcgttcct cgcgccgggt cacgtcagta tggttatcgg caccgacgcc
2221 tataatttta tcgccagcga ttttcatcgt ccgctggtgg ttgctggatt cgaacccctt
2281 gatctactac aaggcgtggt catgctggtg cagcagaaaa tagcggccca cagcaaggta
2341 gagaatcagt atcgtcgagt ggtaccggat gccggtaacc tgctggcgca acaggcgatt
2401 gccgatgtgt tctgtgtcaa cggcgacagc gaatggcgcg gcttaggcgt gattgaatct
2461 tctggcgtgc acctgacgcc ggattatcaa cgattcgatg ccgaagcaca tttccgcccg
2521 gcaccgcagc aggtctgcga tgacccgcgc gcgcgttgtg gtgaggtatt aacgggcaaa
2581 tgtaagccgc atcaatgccc gctgtttggt aacacctgta atcctcaaac cgcgtttggt
2641 gcgctgatgg tttcctccga aggagcgtgc gccgcgtggt atcagtatcg tcagcaggag
2701 agtgaagcgt gaataatatc caactcgccc acggtagcgg cggccaggcg atgcagcaat
2761 taatcaacag cctgtttatg gaagcctttg ccaacccgtg gctggcagag caggaagatc
2821 aggcacgtct tgatctggcg cagctggtag cggaaggcga ccgtctggcg ttctccaccg
2881 acagttacgt tattgacccg ctgttcttcc ctggcggtaa tatcggcaag ctggcgattt
2941 gcggcacagc caatgacgtt gcggtcagtg gcgctattcc gcgctatctc tcctgtggct
3001 ttatcctcga agaaggattg ccgatggaga cactgaaagc cgtagtgacc agcatggcag
3061 aaaccgcccg cgcggcaggc attgccatcg ttactggcga tactaaagtg gtgcagcgcg
3121 gcgcggtaga taaactgttt atcaacaccg ctggcatggg cgcaattccg gcgaatattc
3181 actggggcgc acagacgcta accgcaggcg atgtattgct ggtgagcggt acactcggcg
3241 accacggggc gactatcctt aacctgcgtg agcagctggg gctggatggc gaactggtca
3301 gcgactgcgc ggtgctgacg ccgcttattc agacgctgcg tgacattccc ggcgtgaaag
3361 cgctgcgtga tgccacccgt ggtggtgtaa acgcggtggt tcatgagttc gcggcagcct
3421 gcggttgtgg tattgaactt tcagaagcgg cactgcctgt taaacctgcc gtgcgtggcg
3481 tttgcgaatt gctgggactg gacgccctga actttgccaa cgaaggcaaa ctagtaatag
3541 ctgttgaacg caacgcggca gagcaagtgc tggcagcgtt acattcccat ccactgggga
3601 aagacgcggc gctgattggt gaagtggtgg aacgtaaagg tgttcgtctt gccggtctgt
3661 atggcgtgaa acgaaccctc gatttaccac acgccgaacc gcttccgcgt atatgctaat
3721 aaaattctaa atctcctata gttagtcaat gaccttttgc accgctttgc ggtgctttcc
3781 gtggaagaac aaa
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