GenBank-Updates@genbank.bio.net (05/22/91)
LOCUS CLOPLCG 2576 bp ds-DNA BCT 22-MAY-1991
DEFINITION C. perfringens plc gene for alpha toxin
ACCESSION X17300
KEYWORDS alpha-toxin; phospholipase C.
SOURCE Clostridium perfringens DNA.
ORGANISM Clostridium perfringens
Prokaryota; Bacteria; Firmicutes; Endospore-forming rods and cocci;
Bacillaceae.
REFERENCE 1 (bases 1 to 2576)
AUTHORS Saint-Joanis,B., Garnier,T. and Cole,S.T.
TITLE Gene cloning shows the alpha-toxin of Clostridium perfringens to
contain both sphingomyelinase and lecithinase activities
JOURNAL Mol. Gen. Genet. 219, 453-460 (1989)
STANDARD full automatic
COMMENT From EMBL 26 entry CPPLCG; dated 14-MAR-1991.
FEATURES Location/Qualifiers
prim_transcript complement(<1..(1093.1098))
CDS complement(<1..998)
/note="poorly transcribed in E.coli"
/codon_start=998
-10_signal complement(1104..1109)
/note="minor ORF2 transcript promoter"
-10_signal complement(1108..1113)
/note="major ORF2 transcript promoter"
-35_signal complement(1125..1130)
/note="minor ORF2 transcript promoter"
-35_signal complement(1131..1136)
/note="major ORF2 transcript promoter"
-35_signal 1226..1231
/note="promoter for plc"
-10_signal 1249..1254
/note="also at 1246/47 tg motif increases expression in
E.coli"
prim_transcript 1265..>2576
RBS 1311..1318
transit_peptide 1327..1410
/note="putative"
CDS 1327..2523
/product="alpha-toxin"
/gene="plc"
/evidence=EXPERIMENTAL
/codon_start=1327
mat_peptide 1411..2520
/note="putative 43kDa protein"
BASE COUNT 950 a 365 c 434 g 827 t
ORIGIN
1 aagcttgata tggacttttt agcaatatta acagcctcat ctaggttagg atttagaaga
61 ggaagtagtg cttctgagaa tctagctaag ttccataagg ccatattagg ttgatttcca
121 taggcatatc taccagcata atcaatggaa ctaaatactg tgtttgtatc ataagtatcc
181 ataaatgcac atggtccata atctatagtt tctcctgaga ttaccatatt atcagtgttc
241 ataaccccat gaatgaatcc aacactttgc cacttaacta taagttcagc ttgacgattt
301 attacctctt caagaaataa aatatattta ttttcactat tagctatatt aggaaagtgt
361 cttttaatag tgtagtcagc aagactttta agatcctcta aagtacccca ttgagctgca
421 taagcaaaag ttccaactct aatgtgacta gaagctattc ttgttaatat agcaccttgt
481 tcaaattttt ctcttaaaac ttcctcacca gtacttacta cagcaaggct tctagttgta
541 ggaattccaa gactatgcat accttcactt attatatatt ctctaagcat aggtgcaagg
601 gcagcctttc catctccacc tcttgaatat attgttctac cagacccttt taattgaaca
661 tcatatcttt taccatcttt agttacatgt tctcctaata agagtgctct accatctcct
721 agcattgtaa aatggccaaa ttggtgaccg gcataagcct gtgcaattgg ggtaatacct
781 gggaaagttt catttcctgc aaatatatta agtccaaaat cgctgtttaa aatttcttca
841 ttaagcccaa gttctttagc aagagaagta ttaaacttaa taagtttagg attttttgaa
901 ccctttggat tttgttcact aaagaatata tttggaagag ttaaataagt attttctagg
961 ttaaaacctg tttttgattg aaaattttta ttatccatat taaaatcctt tgccttataa
1021 tttatttcaa attttattcc atcccttata ttatgagtaa aaattcttat taaattaaaa
1081 aacaatattt aacttattat aacactaata attgtaaatt ttcatattaa aaataagttt
1141 aataatttag agtgggtgag tttagatatt ttaaattaaa atttgaattt tattaaaaaa
1201 tatttaaaaa atattcaaaa gtttagtgag gttatgttaa ttatatggta taatttcaat
1261 gcgagtgtta atcgttatca aaaaggggag attaatactt gaaaaaaatt aacgggggat
1321 ataaaaatga aaagaaagat ttgtaaggcg cttgtttgtg ccacgctagt aactagccta
1381 tgggctgggg tatcaactaa agtctacgct tgggatggaa aaattgatgg aacaggaact
1441 catgctatga ttgtaactca aggtgtttca atcttagaaa atgatatgtc caaaaatgaa
1501 ccagaaagtg taagaaaaaa cttagagatt ttaaaagata acatgcatga gcttcaatta
1561 ggttctactt atccagatta tgataagaat gcatatgatc tatatcaaga tcatttctgg
1621 gatcctgata caaataataa tttctcaaag gataatagtt ggtatttagc ttattctata
1681 cctgacacag gggaatcaca aataagaaaa ttttcagcat tagctagata tgaatggcaa
1741 agaggaaatt ataaacaagc tacattctat cttggagaag ctatgcacta ttttggagat
1801 atagatactc catatcatcc tgctaatgtt actgccgttg atagcgcagg acatgttaag
1861 tttgagactt ttgcagaaga aaggaaagaa cagtataaaa taaacacagt aggttgcaaa
1921 actaatgagg atttttatgc tgatatctta aaaaacaaag attttaatgc atggtcaaaa
1981 gaatatgcaa gaggttttgc taaaacaggg aaatcaatat actatagtca tgctagcatg
2041 agtcatagtt gggatgattg ggattatgca gcaaaggtaa ctttagctaa ctctcaaaaa
2101 ggaacagcag gatatattta tagattctta cacgatgtat cagagggtaa tgatccatca
2161 gttggaaata atgtaaaaga actagtagct tacatatcaa ctagtggtga aaaagatgct
2221 ggaacagatg actacatgta ttttggaatc aaaacaaagg atggaaaaac tcaagaatgg
2281 gaaatggaca acccaggaaa tgattttatg gctggaagca aagacactta tactttcaaa
2341 ttaaaagatg aaaatctaaa aattgatgat atacaaaata tgtggattag aaaaagaaaa
2401 tatacagcat tcccagatgc ttataagcca gaaaacataa aggtaatagc aaatggaaaa
2461 gttgtagttg acaaggatat aaatgagtgg atttcaggaa attcaactta taatataaaa
2521 taataaaagt aaaaaaataa ttattggttt tggtggtatt tacaaaataa aagctt
//