GenBank-Updates@genbank.bio.net (05/22/91)
LOCUS RABIG07 642 bp ds-DNA MAM 22-MAY-1991 DEFINITION Rabbit germline gene V18b for immunoglobulin kappa light chain variable region ACCESSION X02336 KEYWORDS immunoglobulin light chain; kappa-immunoglobulin; signal peptide; variable region. SOURCE Oryctolagus cuniculus DNA. ORGANISM Oryctolagus cuniculus Eukaryota; Animalia; Metazoa; Chordata; Vertebrata; Mammalia; Theria; Eutheria; Lagomorpha; Leporidae. REFERENCE 1 (bases 1 to 642) AUTHORS Heidmann,O. and Rougeon,F. TITLE Immunoglobulin kappa light-chain diversity in rabbit is based on the 3' length heterogeneity of germ-line variable genes JOURNAL Nature 311, 74-76 (1984) STANDARD full automatic COMMENT From EMBL 26 entry OCIG07; dated 07-NOV-1985. FEATURES Location/Qualifiers mRNA 7..61 /note="exon 1" CDS 7..61 /note="signal peptide (aa -22 to -5) (61 is 1st base in codon)" /codon_start=7 intron 62..184 /note="intron I" mRNA 185..495 /note="Exon 2" CDS 185..195 /note="signal peptide (aa -4 to -1) (185 is 2nd base in codon)" /codon_start=185 misc_feature 196..264 /note="framework 1 (FR1) (aa 1-23)" CDS 196..495 /note="V18b gene (aa 1-100)" /codon_start=196 misc_feature 265..303 /note="complementarity determining region 1 (cdr 1) (aa 24-36)" misc_feature 304..348 /note="FR2 (aa 37-51)" misc_feature 349..369 /note="CDR2 (aa 52-58)" misc_feature 370..465 /note="FR3 (aa 59-90)" misc_feature 466..495 /note="CDR3 (aa 91-100)" misc_feature 496..502 /note="recombination signal" misc_feature 515..523 /note="recombination signal" BASE COUNT 136 a 179 c 185 g 142 t ORIGIN 1 cccagcatgg acaccagggc ccccactcag ctgctggggc tcctgctgct ctggctccca 61 ggtaaggaag gccagcagca ggaacggcct cgggcagtgt ggccagggct gcccgtctct 121 gcaggagcgt cctcttagaa tgtgaggatg agtgtggtct ctgtgtttct gtttcccttc 181 tcaggtgcca catttgctca agtgctgacc cagactgaat cgcccgtgtc tgcacctgtg 241 ggaggcacag tcaccatcaa ttgccaggcc agtcagagtg tttatgataa caactggtta 301 tcctggtatc agcagaaacc agggcagcct cccaagctcc tgatctatga tgcatccaaa 361 ctggcatctg gggtcccatc acggttcagc ggcagtggat ctgggacaca gttcactctc 421 accatcagtg gcgtacagtg tgacgatgct gccacttact actgtcaagg cagttattat 481 agtagtggtt ggtaccacag tgttagaagc cctaacaaaa ccaccaggaa agcagaagtg 541 tgaggctggg ctgccccagc tgctcctcct ggaccccatc tgctgagagt gattctcaga 601 gcagccaggc ttgaaggtcc tgcagggtcg ggtggaaggg gc //