GenBank-Updates@genbank.bio.net (05/22/91)
LOCUS RABIG09 768 bp ds-DNA MAM 22-MAY-1991
DEFINITION Rabbit germline gene V19b for immunoglobulin kappa light chain
variable region
ACCESSION X02338
KEYWORDS immunoglobulin; immunoglobulin light chain; kappa-immunoglobulin;
signal peptide; variable region.
SOURCE Oryctolagus cuniculus DNA.
ORGANISM Oryctolagus cuniculus
Eukaryota; Animalia; Metazoa; Chordata; Vertebrata; Mammalia;
Theria; Eutheria; Lagomorpha; Leporidae.
REFERENCE 1 (bases 1 to 768)
AUTHORS Heidmann,O. and Rougeon,F.
TITLE Immunoglobulin kappa light-chain diversity in rabbit is based on
the 3' length heterogeneity of germ-line variable genes
JOURNAL Nature 311, 74-76 (1984)
STANDARD full automatic
COMMENT From EMBL 26 entry OCIG09; dated 06-JUL-1989.
FEATURES Location/Qualifiers
misc_feature 37..40
/note="put. CAAT-like sequence"
misc_feature 76..80
/note="put. TATA-like sequence"
misc_feature 101..183
/note="inframe initiation codon"
mRNA 131..185
/note="exon 1"
CDS 131..185
/note="signal peptide (aa -22 to -5) (185 is 1st base in
codon)"
/codon_start=131
intron 186..308
/note="intron I"
mRNA 309..613
/note="exon 2"
CDS 309..319
/note="signal peptide (aa -4 to -1) (309 is 2nd base in
codon)"
/codon_start=309
misc_feature 320..388
/note="FR1 (aa 35-49)"
CDS 320..613
/note="V19b (aa 1-98)"
/codon_start=320
misc_feature 389..421
/note="CDR1 (aa 24-34)"
misc_feature 422..466
/note="FR2 (aa 35-49)"
misc_feature 467..487
/note="CDR2 (aa 50-56)"
misc_feature 488..583
/note="FR3 (aa 57-88)"
misc_feature 584..613
/note="CDR3 (aa 88-98)"
misc_feature 614..620
/note="recombination signal"
misc_feature 633..640
BASE COUNT 149 a 227 c 213 g 152 t 27 others
ORIGIN
1 ggccttcagc tgggcacagc ctgccccacc cctgctcatt tgcatgcccc cagagcaccg
61 cccacctctc tgggcattta ggagcaggct gctccgccct atgcaggagg cagtgcaggc
121 aggacccagc atggacacaa gggcccccac tcagctgctg gggctcctgc tgctctggct
181 cccaggtaag gaaggccnnn nnnnnnnnnn nnnnnnnnnn nnnnggccag ggctgcccat
241 ctctgcagga acgtcctctt agaacgtgag gatgagtgtg gtctctgtgt ttctgtttcc
301 cttctcaggt gccagatgtg ccatcgatat gacccagact ccagcctccg tggaggcagc
361 tgtgggaggc acaatcacca tcaattgcca agccagtgag agcattagca gttggttagc
421 ctggtatcag cagaaaccag ggcagcctcc caagctcctg atctacgaag catccaaact
481 ggcatctggg gtcccatcgc ggttcagtgg cagtggatct gggacacagt tcactctcac
541 catcagcggc gtgcagtgtg acgatgctgc cacttactac tgtcaaggcg gttattataa
601 tagtggttgg taccacagtg ttccaagccc taacaactcc ctcccccccg aggaaagcag
661 aagtgtgagg ctgggctgcc ccagctgctg ctcctgggcc ccatctgctg agagtgattc
721 tcagagcagc caggcttgaa ggtccctact ggggcaggtg gaaggggc
//