GenBank-Updates@genbank.bio.net (05/22/91)
LOCUS PIGANP 474 bp ss-mRNA MAM 22-MAY-1991
DEFINITION Porcine mRNA for prepro atrial natiuretic peptide (ANP) with
human-derived PCR primers
ACCESSION X54669
KEYWORDS atrial naturietic peptide.
SOURCE Sus scrofa RNA.
ORGANISM Sus scrofa
Eukaryota; Animalia; Metazoa; Chordata; Vertebrata; Mammalia;
Theria; Eutheria; Artiodactyla; Suiformes; Suidae.
REFERENCE 1 (bases 1 to 474)
AUTHORS Maegert,H.J.
JOURNAL Unpublished (1990)
STANDARD full automatic
REFERENCE 2 (bases 1 to 474)
AUTHORS Maegert,H.J., Appelhans,H., Gassen,H.G. and Forssmann,W.G.
TITLE Nucleotide sequence of a porcine prepro atrial natiuretic peptide
(ANP) cDNA
JOURNAL Nucleic Acids Res. 18, 6704-6704 (1990)
STANDARD full automatic
COMMENT *source: strain=domesticus; developmental stage=adult; *source:
tissue=heart (right atrium); library=pUC 18;
Data kindly reviewed (28-NOV-1990) by Maegert H.-J.
From EMBL 26 entry SSANP; dated 11-DEC-1990.
FEATURES Location/Qualifiers
mRNA <1..>474
misc_feature 1..23
/standard_name="pcr-primer CDD 1"
sig_peptide 12..83
CDS 12..464
/product="prepro atrial natriuretic"
/codon_start=12
mat_peptide 84..461
/product="pro atrial natriuretic"
mat_peptide 378..461
/product="mature atrial natriuretic"
misc_feature 453..474
/standard_name="pcr-primer CDD 2"
BASE COUNT 101 a 131 c 148 g 94 t
ORIGIN
1 acgacgccag catgagctcc ttcaccatca ccgtgagctt cctcctcgtt ctggtgtttc
61 agttcccagg gcaaaccaga gcgaaccctg tgtacggctc cgtgtccaat gcagacctga
121 tggatttcaa gaatttgctg gaccacttgg aggacaagat gcctttagaa gatgaggcta
181 tgcccccaca agtactaagc gagcagaatg aggaagtggg ggcccctctc agcccccttt
241 tggaggtacc tccctggacc ggggaggtga acccagccca gagagatggg ggtgccctgg
301 ggcggggccc ctgggacgct tccgatagat ctgcccttct gaagagcaag ctgagggcac
361 tgcttgctgc ccctcggagc ctgcggaggt ccagctgctt cgggggtagg atggacagga
421 ttggagcaca gagtggactg ggctgtaaca gcttccggta ctgaagataa cagc
//