GenBank-Updates@genbank.bio.net (05/22/91)
LOCUS PIGANP 474 bp ss-mRNA MAM 22-MAY-1991 DEFINITION Porcine mRNA for prepro atrial natiuretic peptide (ANP) with human-derived PCR primers ACCESSION X54669 KEYWORDS atrial naturietic peptide. SOURCE Sus scrofa RNA. ORGANISM Sus scrofa Eukaryota; Animalia; Metazoa; Chordata; Vertebrata; Mammalia; Theria; Eutheria; Artiodactyla; Suiformes; Suidae. REFERENCE 1 (bases 1 to 474) AUTHORS Maegert,H.J. JOURNAL Unpublished (1990) STANDARD full automatic REFERENCE 2 (bases 1 to 474) AUTHORS Maegert,H.J., Appelhans,H., Gassen,H.G. and Forssmann,W.G. TITLE Nucleotide sequence of a porcine prepro atrial natiuretic peptide (ANP) cDNA JOURNAL Nucleic Acids Res. 18, 6704-6704 (1990) STANDARD full automatic COMMENT *source: strain=domesticus; developmental stage=adult; *source: tissue=heart (right atrium); library=pUC 18; Data kindly reviewed (28-NOV-1990) by Maegert H.-J. From EMBL 26 entry SSANP; dated 11-DEC-1990. FEATURES Location/Qualifiers mRNA <1..>474 misc_feature 1..23 /standard_name="pcr-primer CDD 1" sig_peptide 12..83 CDS 12..464 /product="prepro atrial natriuretic" /codon_start=12 mat_peptide 84..461 /product="pro atrial natriuretic" mat_peptide 378..461 /product="mature atrial natriuretic" misc_feature 453..474 /standard_name="pcr-primer CDD 2" BASE COUNT 101 a 131 c 148 g 94 t ORIGIN 1 acgacgccag catgagctcc ttcaccatca ccgtgagctt cctcctcgtt ctggtgtttc 61 agttcccagg gcaaaccaga gcgaaccctg tgtacggctc cgtgtccaat gcagacctga 121 tggatttcaa gaatttgctg gaccacttgg aggacaagat gcctttagaa gatgaggcta 181 tgcccccaca agtactaagc gagcagaatg aggaagtggg ggcccctctc agcccccttt 241 tggaggtacc tccctggacc ggggaggtga acccagccca gagagatggg ggtgccctgg 301 ggcggggccc ctgggacgct tccgatagat ctgcccttct gaagagcaag ctgagggcac 361 tgcttgctgc ccctcggagc ctgcggaggt ccagctgctt cgggggtagg atggacagga 421 ttggagcaca gagtggactg ggctgtaaca gcttccggta ctgaagataa cagc //