GenBank-Updates@genbank.bio.net (05/22/91)
LOCUS ECORRNBZ 7508 bp ds-DNA BCT 22-MAY-1991
DEFINITION E. coli ribosomal operon rrnB encoding the 16S ribosomal RNA. Also
transfer RNA specific for Glu, 23S ribosomal RNA and two
unidentified open reading frames. This sequence was obtained from
the transducing phage lambda-rif-d 18 (BAMHI fragment).
ACCESSION V00348
KEYWORDS 16S ribosomal RNA; 23S ribosomal RNA; ribosomal RNA; transfer RNA;
transfer RNA-Glu; unidentified reading frame.
SOURCE Escherichia coli DNA.
ORGANISM Escherichia coli
Prokaryota; Bacteria; Gracilicutes; Scotobacteria;
Facultatively anaerobic rods; Enterobacteriaceae.
REFERENCE 1 (bases 1 to 7508)
AUTHORS Brosius,J., Dull,T.J., Sleeter,D.D. and Noller,H.F.
TITLE Gene organization and primary structure of a ribosomal RNA operon
from Escherichia coli
JOURNAL J. Mol. Biol. 148, 107-127 (1981)
STANDARD full automatic
COMMENT From EMBL 26 entry ECRRNBZ; dated 18-MAR-1991.
FEATURES Location/Qualifiers
misc_feature 240..254
/note="lambda attachment site"
CDS 275..1141
/note="unidentified reading frame I"
/codon_start=275
rRNA 1518..2959
/note="16S rRNA"
tRNA 3231..3306
/note="transfer RNA - Glu - 2"
rRNA 3500..6403
/note="23S rRNA"
BASE COUNT 1969 a 1694 c 2165 g 1678 t 2 others
ORIGIN
1 ggatccgcct acctttcacg agttgcgcag tttgtctgca agactctatg agaagcagat
61 aagcgataag tttgctcaac atcttctcgg gcataagtcg gacaccatgg catcacagta
121 tcgtgatgac agaggcaggg agtgggacaa aattgaaatc aaataatgat tttattttga
181 ctgatagtga cctgttcgtt gcaacaaatt gataagcaat gcttttttat aatgccaact
241 tagtataaaa tagccaacct gttcgacaaa gattatgaga cagtctatgg ctaccaaact
301 gcaggacggg aatacacctt gtctggcagc tacaccttct gaaccacgtc ccaccgtgct
361 ggtgtttgac tccggcgtcg gtgggttgtc ggtctatgac gagatccggc atctcttacc
421 ggatctccat tacatttatg ctttcgataa cgtcgctttc ccgtatggcg aaaaaagcga
481 agcgtttatt gttgagcgag tggtggcaat tgtcaccgcg gtgcaagaac gttatcccct
541 tgcgctggct gtggtcgctt gcaacactgc cagtaccgtt tcacttcctg cattacgcga
601 aaagttcgac ttcccggttg ttggtgtcgt gccggcgatt aaacctgctg cacgtctgac
661 ggcaaatggc attgtcggat tactggcaac ccgcggaaca gttaaacgtt cttatactca
721 tgagctgatc gcgcgtttcg ctaatgaatg ccagatagaa atgctgggct cggcagagat
781 ggttgagttg gctgaagcga agctacatgg cgaagatgtt tctctggatg cactaaaacg
841 tatcctacgc ccgtggttaa gaatgaaaga gccgccagat accgttgtat tgggttgcac
901 ccatttccct ctactacaag aagaactgtt acaagtgctg ccagagggaa cccggctggt
961 ggattctggc gcagcgattg ctcgccgaac ggcctggttg ttagaacatg aagccccgga
1021 tgcaaaatct gccgatgcga atattgcctt ttgtatggca atgacgccag gagctgaaca
1081 attattgccc gttttacagc gttacggctt cgaaacgctc gaaaaactgg cagttttagg
1141 ctgatttggt tgaatgttgc gcggtcagaa aattatttta aatttcctct tgtcaggccg
1201 gaataactcc ctataatgcg ccaccactga cacggaacaa cggcaaacac gccgccgggt
1261 cagcggggtt ctcctgagaa ctccggcaga gaaagcaaaa ataaatgctt gactctgtag
1321 cgggaaggcg tattatgcac accccgcgcc gctgagaaaa agcgaagcgg cactgctctt
1381 taacaattta tcagacaatc tgtgtgggca ctcgaagata cggattctta acgtcgcaag
1441 acgaaaaatg aataccaagt ctcaagagtg aacacgtaat tcattacgaa gtttaattct
1501 ttgagcgtca aacttttaaa ttgaagagtt tgatcatggc tcagattgaa cgctggcggc
1561 aggcctaaca catgcaagtc gaacggtaac aggaagaagc ttgcttcttt gctgacgagt
1621 ggcggacggg tgagtaatgt ctgggaaact gcctgatgga gggggataac tactggaaac
1681 ggtagctaat accgcataac gtcgcaagac caaagagggg gaccttcggg cctcttgcca
1741 tcggatgtgc ccagatggga ttagctagta ggtggggtaa cggctcacct aggcgacgat
1801 ccctagctgg tctgagagga tgaccagcca cactggaact gagacacggt ccagactcct
1861 acgggaggca gcagtgggga atattgcaca atgggcgcaa gcctgatgca gccatgccgc
1921 gtgtatgaag aaggccttcg ggttgtaaag tactttcagc ggggaggaag ggagtaaagt
1981 taataccttt gctcattgac gttacccgca gaagaagcac cggctaactc cgtgccagca
2041 gccgcggtaa tacggagggt gcaagcgtta atcggaatta ctgggcgtaa agcgcacgca
2101 ggcggtttgt taagtcagat gtgaaatccc cgggctcaac ctgggaactg catctgatac
2161 tggcaagctt gagtctcgta gaggggggta gaattccagg tgtagcggtg aaatgcgtag
2221 agatctggag gaataccggt ggcgaaggcg gccccctgga cgaagactga cgctcaggtg
2281 cgaaagcgtg gggagcaaac aggattagat accctggtag tccacgccgt aaacgatgtc
2341 gacttggagg ttgtgccctt gaggcgtggc ttccggagct aacgcgttaa gtcgaccgcc
2401 tggggagtac ggccgcaagg ttaaaactca aatgaattga cgggggcccg cacaagcggt
2461 ggagcatgtg gtttaattcg atgcaacgcg aagaacctta cctggtcttg acatccacgg
2521 aagttttcag agatgagaat gtgccttcgg gaaccgtgag acaggtgctg catggctgtc
2581 gtcagctcgt gttgtgaaat gttgggttaa gtcccgcaac gagcgcaacc cttatccttt
2641 gttgccagcg gtccggccgg gaactcaaag gagactgcca gtgataaact ggaggaaggt
2701 ggggatgacg tcaagtcatc atggccctta cgaccagggc tacacacgtg ctacaatggc
2761 gcatacaaag agaagcgacc tcgcgagagc aagcggacct cataaagtgc gtcgtagtcc
2821 ggattggagt ctgcaactcg actccatgaa gtcggaatcg ctagtaatcg tggatcagaa
2881 tgccacggtg aatacgttcc cgggccttgt acacaccgcc cgtcacacca tgggagtggg
2941 ttgcaaaaga agtaggtagc ttaaccttcg ggagggcgct taccactttg tgattcatga
3001 ctggggtgaa gtcgtaacaa ggtaaccgta ggggaacctg cggttggatc acctccttac
3061 cttaaagaag cgtactttgt agtgctcaca cagattgtct gatagaaagt gaaaagcaag
3121 gcgtttacgc gttgggagtg aggctgaaga gaataaggcc gttcgctttc tattaatgaa
3181 agctcaccct acacgaaaat atcacgcaac gcgtgataag caattttcgt gtccccttcg
3241 tctagaggcc caggacaccg ccctttcacg gcggtaacag gggttcgaat cccctagggg
3301 acgccacttg ctggtttgtg agtgaaagtc gccgacctta atatctcaaa actcatcttc
3361 gggtgatgtt tgagatnttt gctctttaaa aatctggatc aagctgaaaa ttgaaacact
3421 gaacaacgag agttgttcgt gagtctctca aattttcgca acacgatgat gaatcgaaag
3481 aaacatcttc gggttgtgag gttaagcgac taagcgtaca cggtggatgc cctggcagtc
3541 agaggcgatg aaggacgtgc taatctgcga taagcgtcgg taaggtgata tgaaccgtta
3601 taaccggcga tttccgaatg gggaaaccca gtgtgtttcg acacactatc attaactgaa
3661 tccataggtt aatgaggcga accgggggaa ctgaaacatc taagtacccc gaggaaaaga
3721 aatcaaccga gattccccca gtagcggcga gcgaacgggg agcagcccag agcctgaatc
3781 agtgtgtgtg ttagtggaag cgtctggaaa ggcgcgcgat acagggtgac agccccgtac
3841 acaaaaatgc acatgctgtg agctcgatga gtagggcggg acacgtggta tcctgtctga
3901 atatgggggg accatcctcc aaggctaaat actcctgact gaccgatagt gaaccagtac
3961 cgtgagggaa aggcgaaaag aaccccggcg aggggagtga aaaagaacct gaaaccgtgt
4021 acgtacaagc agtgggagca cgcttaggcg tgtgactgcg taccttttgt ataatgggtc
4081 agcgacttat attctgtagc aaggttaacc gaatagggga gccgaaggga aaccgagtct
4141 taactgggcg ttaagttgca gggtatagac ccgaaacccg gtgatctagc catgggcagg
4201 ttgaaggttg ggtaacacta actggaggac cgaaccgact aatgttgaaa aattagcgga
4261 tgacttgtgg ctgggggtga aaggccaatc aaaccgggag atagctggtt ctccccgaaa
4321 gctatttagg tagcgcctcg tgaattcatc tccgggggta gagcactgtt tcggcaaggg
4381 ggtcatcccg acttaccaac ccgatgcaaa ctgcgaatac cggagaatgt tatcacggga
4441 gacacacggc gggtgctaac gtccgtcgtg aagagggaaa caacccagac cgccagctaa
4501 ggtcccaaag tcatggttaa gtgggaaacg atgtgggaag gcccagacag ccaggatgtt
4561 ggcttagaag cagccatcat ttaaagaaag cgtaatagct cactggtcga gtcggcctgc
4621 gcggaagatg taacggggct aaaccatgca ccgaagctgc ggcagcgacg cttatgcgtt
4681 gttgggtagg ggagcgttct gtaagcctgc gaaggtgtgc tgtgaggcat gctggaggta
4741 tcagaagtgc gaatgctgac ataagtaacg ataaagcggg tgaaaagccc gctcgccgga
4801 agaccaaggg ttcctgtcca acgttaatcg gggcagggtg agtcgacccc taaggcgagg
4861 ccgaaaggcg tagtcgatgg gaaacaggtt aatattcctg tacttggtgt tactgcgaag
4921 gggggacgga gaaggctatg ttggccgggc gacggttgtc ccggtttaag cgtgtaggct
4981 ggttttccag gcaaatccgg aaaatcaagg ctgaggcgtg atgacgaggc actacggtgc
5041 tgaagcaaca aatgccctgc ttccaggaaa agcctctaag catcaggtaa catcaaatcg
5101 taccccaaac cgacacaggt ggtcaggtag agaataccaa ggcgcttgag agaactcggg
5161 tgaaggaact aggcaaaatg gtgccgtaac ttcgggagaa ggcacgctga tatgtaggtg
5221 aggtccctcg cggatggagc tgaaatcagt cgaagatacc agctggctgc aactgtttat
5281 taaaaacaca gcactgtgca aacacgaaag tggacgtata cggtgtgacg cctgcccggt
5341 gccggaaggt taattgatgg ggttagcgca agcgaagctc ttgatcgaag ccccggtaaa
5401 cggcggccgt aactataacg gtcctaaggt agcgaaattc cttgtcgggt aagttccgac
5461 ctgcacgaat ggcgtaatga tggccaggct gtctccaccc gagactcagt gaaattgaac
5521 tcgctgtgaa gatgcagtgt acccgcggca agacggaaag accccgtgaa cctttactat
5581 agcttgacac tgaacattga gccttgatgt gtaggatagg tgggaggctt tgaagtgtgg
5641 acgccagtct gcatggagcc gaccttgaaa taccaccctt taatgtttga tgttctaacg
5701 ttgacccgta atccgggttg cggacagtgt ctggtgggta gtttgactgg ggcggtctcc
5761 tcctaaagag taacggagga gcacgaaggt tggctaatcc tggtcggaca tcaggaggtt
5821 agtgcaatgg cataagccag cttgactgcg agcgtgacgg cgcgagcagg tgcgaaagca
5881 ggtcatagtg atccggtggt tctgaatgga agggccatcg ctcaacggat aaaaggtact
5941 ccggggataa caggctgata ccgcccaaga gttcatatcg acggcggtgt ttggcacctc
6001 gatgtcggct catcacatcc tggggctgaa gtaggtccca agggtatggc tgttcgccat
6061 ttaaagtggt acgcgagctg ggtttagaac gtcgtgagac agttcggtcc ctatctgccg
6121 tgggcgctgg agaactgagg ggggctgctc ctagtacgag aggaccggag tggacgcatc
6181 actggtgttc gggttgtcat gccaatggca ctgcccggta gctaaatgcg gaagagataa
6241 gtgctgaaag catctaagca cgaaacttgc cccgagatga gttctccctg accctttaag
6301 ggtcctgaag gaacgttgaa gacgacgacg ttgataggcc gggtgtgtaa gcgcagcgat
6361 gcgttgagct aaccggtact aatgaaccgt gaggcttaac cttacaacgc cgaagctgtt
6421 ttggcggatg agagaagatt ttcagcctga tacagattaa atcagaacgc agaagcggtc
6481 tgataaaaca gaatttgcct ggcggcagta gcgcggtggt cccacctgac cccatgccga
6541 actcagaagt gaaacgccgt agcgccgatg gtagtgtggg gtctccccat gcgagagtag
6601 ggaactgcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt
6661 atctgttgtt tgtcggtgaa cgctctcctg agtaggacaa atccgccggg agcggatttg
6721 aacgttgcga agcaacggcc cggagggtgg cgggcaggac gcccgccata aactgccagg
6781 catcaaatta agcagaaggc catcctgacg gatggccttt ttgcgtttct acaaactctt
6841 cctgtcgtca tatctacaag ccatcccccc acagatacgg taaactagcc tcgtttttgc
6901 atcaggaaag cagctatgaa ccactcctta aaaccctgga acacatttgg cattgatcat
6961 aatgctcagc acattgtatg tgccgaagac gaacaacaat tactcaatnc ctggcagtat
7021 gcaaccgcag aaggacaacc cgttcttatt ctgggtgaag gaagtaatgt actttttctg
7081 gaggactatc gcggcacggt gatcatcaac cggatcaaag gtatcgaaat tcatgatgaa
7141 cctgatgcgt ggtatttaca tgtaggagcc ggagaaaact ggcatcgtct ggtaaaatac
7201 actttgcagg aaggtatgcc tggtctggaa aatctggcat taattcctgg ttgtgtcggc
7261 tcatcaccta tccagaatat tggtgcttat ggcgtagaat tacagcgagt ttgcgcttat
7321 gttgattctg ttgaactggc gacaggcaag caagtgcgct taactgccaa agagtgccgt
7381 tttggctatc gcgacagtat ttttaaacat gaataccagg accgcttcgc tattgtagcc
7441 gtaggtctgc gtctgccaaa agagtggcaa cctgtactaa cgtatggtga cttaactcgt
7501 ctggatcc
//