GenBank-Updates@genbank.bio.net (05/22/91)
LOCUS BLYAMY152 2625 bp ds-DNA PLN 22-MAY-1991
DEFINITION Barley Amy gene for alpha amylase (clone gRAmy152) (EC 3.2.1.1)
ACCESSION X15226
KEYWORDS amylase-alpha.
SOURCE Hordeum vulgare DNA.
ORGANISM Hordeum vulgare
Eukaryota; Plantae; Embryobionta; Magnoliophyta; Liliopsida;
Commelinidae; Cyperales; Poaceae.
REFERENCE 1 (bases 1 to 2625)
AUTHORS Rahmatullah,R.J., Huang,J.K., Clark,K.L., Reeck,G.R., Chandra,G.R.
and Muthukrishnan,S.
TITLE Nucleotide and predicted amino acid sequences of two different
genes for high-pI alpha-amylases from barley
JOURNAL Plant Mol. Biol. 12, 119-121 (1989)
STANDARD full automatic
COMMENT SWISS-PROT; P04063; AMY2$HORVU.
*source: library=lambda Charon 35; developmental stage=embryo
From EMBL 26 entry HVAMY152; dated 19-MAR-1991.
FEATURES Location/Qualifiers
promoter 620..627
/note="TATA-box"
precursor_RNA 650..>2159
/note="gRAmy152 transcript"
mRNA 650..792
/note="exon 1"
CDS 706..792
/note="alpha-amylase"
/codon_start=706
intron 793..860
/note="intron I"
mRNA 861..1805
/note="exon 2"
CDS 861..1805
/note="alpha amylase"
/codon_start=861
intron 1806..1907
/note="intron II"
mRNA 1908..>2159
/note="exon 3"
CDS 1908..2156
/note="alpha amylase"
/codon_start=1908
misc_feature 2293..2298
/note="put. polyA signal"
BASE COUNT 678 a 700 c 651 g 596 t
ORIGIN
1 ttcacacatt caaaaaaagc aaatttcaaa atttgtactc gaatttaaaa aaagatcatt
61 gatttaaaaa atttcgctaa gtaaaaaact gtccacgtat ttcaaaaaaa gataagtggg
121 catcaatcgg caaaatttga tatgtcaggg tattgagcca tattaatttg gttgtggcac
181 aatatttttg tcccccgttg caacgcacgg ttatttttgt tagtgtgttc ttatggatat
241 atacgcccat aaagtttaag caatacaaca actatttcac caatccctct attctctttc
301 ttcacagcag atagaaggtt ttgtaattgt aaccacagca cactattcga tgaaaaatgc
361 atcgaatgtt ctgtcctcag aaaaacagag gttgaggata actgacggtc gtattgatcc
421 ggtgccttct tatggaaggc caaggctgcc tccatctaca tcacttggga cattgaatcg
481 ccttttgagc tcaccgtacc ggccgataac aaactccggc tgacatatcc actggcccaa
541 aggagcattg aagccgagca cacgagaaag tgatttgcaa gttgcacacc ggcagcaatt
601 ccggcatgct gcagcacact ataaatacct ggccagacac acaagttgaa tgcatcagtt
661 ctccatcgta ctcttcgaga gcacagcaag agagagctga agaacatggc gaacaaacat
721 ttgtccctct ccctcttcct cgtcctcctt ggcctgtcgg ccagcttggc ctccgggcaa
781 gtcctctttc aggtaagatc ttgtcctatc ttcagattct gtatgtaccg cggtcatgtt
841 ttgggttctg catgcgacag ggcttcaact gggagtcgtg gaagcacaat ggcgggtggt
901 acaacttcct gatgggcaag gtggacgaca tcgccgccgc cggcatcacg cacgtctggc
961 tccctccggc gtcgcagtcc gtcgccgagc aagggtacat gccgggccgg ctgtacgacc
1021 tggacgcctc caagtacggc aacaaggcgc agctcaagtc cctcatcggg gcgctccacg
1081 gcaagggcgt caaggccatc gccgacatcg tcatcaacca ccgcacggcg gagcacaagg
1141 acggccgggg catctactgc atcttcgagg gcgacacccc cgacgcccgc ctcgactggg
1201 ggccccacat gatctgccgc gacgaccggc cctacgctga cggcaccggc aacccggaca
1261 ccggcgccga cttcggggcc gcccccgaca tcgaccacct caacctgcgc gtccagaagg
1321 agctcgtcga gtggctcaac tggctcaagg ccgacatcgg cttcgacggc tggcgcttcg
1381 acttcgccaa gggctactcc gcggacgtcg ccaagattta cattgaccgc tcggagccca
1441 gcttcgccgt ggccgagata tggacgtcgc tcgcgtacgg cggggacggc aagcccaacc
1501 tcaaccagga ccagcaccgg caggagctgg tgaactgggt ggacaaggtt ggcggcaaag
1561 ggcccgctac cacgttcgac ttcaccacca agggcatcct caacgtggcc gtggagggcg
1621 agctgtggcg gctgcgcggc acagacggta aggcgccagg catgatcggg tggtggccgg
1681 ccaaggcggt gacctttgtg gacaaccacg acaccggctc cacgcagcac atgtggccct
1741 tcccttctga cagggtcatg cagggatatg cctacatcct cacgcaccca gggacgccat
1801 gcatcgtgag ttcgtctacc aatacatcac atctcaattt tcttttcttg tttcttcata
1861 ataataacaa acatgaccga aatgatgaaa aataatttgg ttctcagttc tacgatcatt
1921 tcttcgactg gggcctgaag gaggagatcg atcgcttggt gtcagtcagg acccggcacg
1981 ggatacacaa cgagagcaag ctgcaaatca tagaggccga cgccgacctt tatctcgccg
2041 agatcgacgg caaggtcatc gtcaagctcg ggccaagata cgatgtgggg aacctcattc
2101 cgggaggctt caaggtggcc gcgcacggca atgactatgc cgtatggcag aaaatatgag
2161 caaaattgcg agagcagctc tacaagttcc tatatgatac atattagtcc gagctaacgc
2221 gttcacatag tacaatttaa tacttcctcc atgtaaaagt gaggatgagg gacatgcatt
2281 gtatttttga taaaataatg atttataaga tttgattttc gcacctcttt ctttctttaa
2341 taagacaaca aaatgagcat cttagatcag attaatatcg aggaaaataa aatccactac
2401 aatggataga taaccaaaag ataaaattac aataaaacgc ataccggtct ttttctttct
2461 ctgattttcg gcactcttgg tttaccattg ttagttgaac gaaaactggg caaccatgac
2521 cagtaactcg tacgggagca tacggaattg tgtcacgggg ctgagctgag gcgcacaaaa
2581 gcaatggcgg ggtttcctct ttttggccca gagtcgttgg agctc
//GenBank-Updates@genbank.bio.net (05/22/91)
LOCUS BLYAMY56 2203 bp ds-DNA PLN 22-MAY-1991
DEFINITION Barley Amy gene for alpha amylase (clone gRAmy56) (EC 3.2.1.1)
ACCESSION X15227
KEYWORDS amylase-alpha.
SOURCE Hordeum vulgare DNA.
ORGANISM Hordeum vulgare
Eukaryota; Plantae; Embryobionta; Magnoliophyta; Liliopsida;
Commelinidae; Cyperales; Poaceae.
REFERENCE 1 (bases 1 to 2203)
AUTHORS Rahmatullah,R.J., Huang,J.K., Clark,K.L., Reeck,G.R., Chandra,G.R.
and Muthukrishnan,S.
TITLE Nucleotide and predicted amino acid sequences of two different
genes for high-pI alpha-amylases from barley
JOURNAL Plant Mol. Biol. 12, 119-121 (1989)
STANDARD full automatic
COMMENT SWISS-PROT; P04750; AMY6$HORVU.
*source: library=lambda Charon 35; developmental stage=embryo
From EMBL 26 entry HVAMY56; dated 19-MAR-1991.
FEATURES Location/Qualifiers
promoter 413..420
/note="TATA-box"
precursor_RNA 443..>2001
/note="gRAmy56 transcript"
mRNA 443..591
/note="exon 1"
CDS 505..591
/note="alpha-amylase"
/codon_start=505
intron 592..692
/note="intron I"
mRNA 693..1643
/note="exon 2"
CDS 693..1643
/note="alpha amylase"
/codon_start=693
intron 1644..1749
/note="intron II"
mRNA 1750..>2001
/note="exon 3"
CDS 1750..1999
/note="alpha amylase"
/codon_start=1750
misc_feature 2121..2126
/note="put. polyA signal"
BASE COUNT 510 a 653 c 587 g 453 t
ORIGIN
1 aaactaaaca acgggcaaat atgtccgttt accgctcaaa aaacgcacct catccttcaa
61 gttaatcaag aaaatttgcc aagaattttc tgaacccgga tttctgcttt gttaacctga
121 aatgcggcaa gtaaccctca gttgcctgca gatcttacgg tgcaacagga taactgacag
181 gaaatgtctg agttctgagg gaattctgaa gttcaggagg ataactgaca gtcgtactgg
241 ccggtgcctt cttgtcgaag gctggatcca tcagtcgcct tttgagctca ccgcaccggc
301 cgataacaaa ctccggccga catatccatc gatgtaccgg cccaacggag cattgaagcc
361 gaacacaccg gaatatgttc tgcaagttgc ccaccggcat gctccagcac actatatata
421 cctggccaga cacaccagct gaatccatca gttctccgtc ctcatcttcc agagcacagc
481 tagctagagc tagagctcaa gatcatggcg aacaaacaca tgtccctttc tctcttcatc
541 gtcctccttg gcctctcgtg cagcttggcc tccgggcaag tcctgtttca ggtaagaata
601 cgatcttgat catcttgtcc gtcggcaagc gcgtgtccgc tcctgggttt tgtacgtact
661 cactgagctt tgggttctgc tgcgttcgac agggttttaa ctgggagtcg tggaagcaca
721 atggcgggtg gtacaacttc ctgatgggca aggtggacga catcgccgcc gctggcgtca
781 cgcacgtgtg gctccccccg gcgtcgcagt ccgtcgccga gcaagggtac atgccgggcc
841 ggctctacga cctggacgcc tccaagtacg gcaacaaggc gcagctcaag tccctcatcg
901 gcgcgctcca cggcaaggcc gtcaaggcca tcgccgacat cgtcatcaac caccgcacgg
961 cggagcgcaa ggacggccgg ggcatctact gcatcttcga gggcggcacc ccggacgcgc
1021 gcctcgactg gggcccccac atgatctgcc gcgacgaccg gccctaccct gacggcaccg
1081 gcaaccggcc aacccggaca cgcgccgact tcggggccgc gccggacatc gaccacctca
1141 acccgcgcgt ccagaaggag ctcgtcgagt ggctcaactg gctcaggacc gacgatggct
1201 tcgacggctg gcgcttcgac ttcgccaagg gctactccgc ggacgtggcc aagatctacg
1261 tcgaccgctc cgagcccagc ttcgccgtcg ccgagatatg gacgtcgctg gcgtacggcg
1321 gggacggcaa gccgaacctc aaccaggacc cgcaccggca ggagctggtg aactgggtga
1381 acaaggtggg cggctccggc cccgccacca cgttcgactt caccaccaag ggcatcctca
1441 acgtggccgt ggagggcgag ctgtggcggc tgcgcggcac cgacggcaag gcgccgggca
1501 tgatcgggtg gtggccggcc aaggcggtga cctttgtcga caaccacgac accggctcca
1561 cgcagcacat gtggcccttc ccttccgaca gggtcatgca gggatatgcc tacatcctca
1621 cgcacccagg gaacccatgc atcgtgagcg tcatcctacc aatacatcat atcaaaatct
1681 tctgttgttt tttccgttca taacaagaaa tcatgaccga actgatggaa aataattgtg
1741 attcttcagt tctacgatca tttcttcgac tggggcttga aggaggagat cgatcgtctg
1801 gtgtcaatca ggacccgaca ggggatacac agtgagagca agctgcagat catggaggcc
1861 gacgccgacc tttaccttgc cgagatcgag ggcaaggtca tcgtcaagct cgggccaaga
1921 tacgatgtcg gacacctcat tcctgaaggc ttcaaggtgg tcgcgcatgg caatgactat
1981 gccgtatggg agaaagtata aagcaaaatt aacggagcgg ctctacaaat tagtccgagc
2041 tcgtgttgtc cacatagtac gattttagta cttcctccat gtaaaaaagg aggatgaggg
2101 acatccattg tatttttcat aaataataca ataatcaata agcttttcgc tacccatggt
2161 ttagtcgatg ttcgtttacc acaaaagtgt aactcgcaag ctt
//