GenBank-Updates@genbank.bio.net (05/22/91)
LOCUS RICALAM 4276 bp ds-DNA PLN 22-MAY-1991
DEFINITION Rice alpha-amylase gene
ACCESSION X16509
KEYWORDS amylase-alpha.
SOURCE Oryza sativa DNA.
ORGANISM Oryza sativa
Eukaryota; Plantae; Embryobionta; Magnoliophyta; Liliopsida;
Commelinidae; Cyperales; Poaceae.
REFERENCE 1 (bases 1 to 4276)
AUTHORS Rodriguez,R.L.
JOURNAL Unpublished (1989)
STANDARD full automatic
REFERENCE 2 (bases 1 to 4276)
AUTHORS Huang,N., Sutliff,T.D., Litts,J.C. and Rodriguez,R.L.
TITLE Classification and characterisation of the rice alpha-amylase
multigene family
JOURNAL Plant Mol. Biol. 14, 655-668 (1990)
STANDARD full automatic
COMMENT SWISS-PROT; P17654; AMY$ORYSA.
*source: strain=Japonica M202; clone=lambda OSg2; library=EMBL4;
Data kindly reviewed (02-JUL-1990) by Rodriguez R.L.
From EMBL 26 entry OSALAM; dated 19-MAR-1991.
FEATURES Location/Qualifiers
mRNA <2474..2473
/note="exon 1"
promoter 2299..2306
/note="put. TATA box"
precursor_RNA 2333..4169
/note="put. primary transcript"
CDS 2384..2473
/note="alpha-amylase (AA 1-30)"
/codon_start=2384
intron 2474..2581
/note="intron I"
mRNA 2582..2714
/note="exon 2"
CDS 2582..2714
/note="alpha-amylase (AA 31-74) (2714 is 1st base in
codon)"
/codon_start=2582
intron 2715..2807
/note="intron II"
mRNA 2808..3619
/note="exon 3"
CDS 2808..3619
/note="alpha-amylase (AA 75-345) (3620 is 2nd base in
codon)"
/codon_start=2808
intron 3620..3703
/note="intron III"
mRNA 3704..>3703
/note="exon 4"
CDS 3704..3952
/note="alpha-amylase (AA 346-428)"
/codon_start=3704
misc_feature 4137..4142
/note="put. polyA signal"
misc_feature 4150..4155
/note="put. alt. polyA signal"
BASE COUNT 1109 a 1076 c 935 g 1156 t
ORIGIN
1 gcatgcgaga ggcacggggt tcgattcccc gcgtctccat cggcactgtt ttttaacatc
61 aaacgctgtt cgatccacta tctgttaatt tcgcaaacac aactaaatct tttttttttt
121 ttgccggtgc gtgcagtgtg acgtccaagg catggcgcat tggcgcctcc cctctttccc
181 ttgatctttt catcagttcg ttcttcttgc agaaaagctg ttctgttaag tcggttccga
241 tctgctcttg ggctcttgcc agaaacaacc tgtgtacgcc agacttatca agccaaccat
301 cctgatgagc ctctgcttat acaagccttt gactccaaaa aggacgagga ggcttgcagc
361 cgcacggaaa taagccgacc gatcctttat tgctctatct ttttcccttg gaataaaaaa
421 cagcccaatt aaaatctggg atgaaactat ggctagctgt tcgcggtgtc agttctcggg
481 acgctaccgt tgttttgttt gaaccggaat gttcagggcg gttcacacca tagacttgga
541 gccaagtggt tccatccaca aaattttctc atcttgaata ttctgttatc tgcctcgaca
601 gacgcaccat atcctgtgtt caggaatgaa tgtgctacag ccaacgtgct gcatgaaatt
661 tgctgaaatc gtgctaaaat gtgcatggca acaggaacct gatgccctgg tcctgtggaa
721 ctgccacggg aaagtatttt ttatagctag gtgcaatcgt atctaggtgt atacatgtca
781 cctacatagc tactcccctt tatcttaaaa tataataatt tttaactctc agtatttgtc
841 ctaaaatata acaaattctc catcaacatt atcttcccaa ccaatcacaa cccttcatca
901 ttaatttttt ccccctacct ccactactca tctaatcaca accctccaac actcacttct
961 atctactttc ttaataactg tcttcaaccc taaaacttct tatattttag gacggaggga
1021 gtatctaaat atttcataaa aaaaatgtta agatagataa agaagatata aacccactat
1081 gcaaacatgc acatcaaaat ttaatttaca gtaaagaaac agaaataaca tattctattt
1141 gtgctggaga tgtactgttc acaatattgt ttttttattt tttatttatc tgattatata
1201 tctgtttcag ccttgcatgg ttgtgtatgt ttgtgtatag acttatgcca ttgtgattga
1261 tgctaccaat tattttcaga ctattttttt atagaggaat tttatagttc ttgagaaaat
1321 accttgaagt atctaaattt tacactaaaa ttgttggtac cttgaggtac aaagtaccta
1381 gaggtaccaa attttactag aaaattgtgg cacctttagg taccttctca aaaatagtac
1441 aattatgggc cgttttggat ttagtgccaa aacgtgctct acaaatattt tgatagtttg
1501 aacagtgcat aagacgggtt tggtttgaag ccaaatcatt ggcattgcca atgtccaatt
1561 tgatattttc tatattatgc taaaagcttg gttctaaatt ggcctccaac caaatacaac
1621 tctactctac caaaaaattt gtagtgccaa aacttgccta ggttttgtca ctaccaacat
1681 tttggtaagt attaaaccaa acaagcccta cattttttta tgtacattta agttgtatgt
1741 aaatgatggg tgcggttgca cctaggtgaa aaaaaataca tattcgccac aactcgcaac
1801 atgtaccaat tcagcagcaa gtgtaagaga gaagatttct ctcgttttac acgcgcacgt
1861 tcaattcctg aactactaaa cggtatgatt ttttgcaaaa attttctata ggaaagttac
1921 ttaaaaatta tattaatcta tttttaaaat ttaaaatagt taatactcaa ttaattatac
1981 gttaatggct cagctcgttt tgcgtacatt ctcaatcgat tcttttcctc tgctctcaaa
2041 tgctctgtgt gcgatcaggt attcatgttc agctcgcaca agcacaagca agacagatgg
2101 aattcctact gacctgcgcc ttttgagtcg ctccaactct caaagtctca aggccattaa
2161 attgcctatg ggctcaccag ccaataacaa actccggctg ttatccatcc aatccagtgt
2221 cccaaagcaa cattcaagcc cagccaggcc tccaaaagtt gcaagttgag catggcaaaa
2281 tccccggcaa ttctcgacta taaatacctg accagacaca cccaggagct tcatcaatca
2341 tccatctccg aagtgtgtct gcagcatgca ggtgctgaac accatggtga acaaacactt
2401 cttgtccctt tcggtcctca tcgtcctcct tggcctctcc tccaacttga cagccgggca
2461 agtcctgttt caggtaagag atcgccatga gttgggtttc aggcttcagt gaactgatcg
2521 ggttttgtac tgagcctaag agaatgatgc agtgatgctc ttgtgtttga tgatgatgca
2581 gggattcaac tgggagtcgt ggaaggagaa tggcgggtgg tacaacttcc tgatgggcaa
2641 ggtggacgac atcgccgcag ccggcatcac ccacgtctgg ctccctccgc cgtctcactc
2701 tgtcggcgag caaggtgcgg tgctctgctc tctcgatccc ctcgtcgtcg caccattgcc
2761 ggcaaaatac atgcacaggt cgttgaattg cttgaatgct tctgcaggct acatgcctgg
2821 gcggctgtac gatctggacg cgtctaagta cggcaacgag gcgcagctca agtcgctgat
2881 cgaggcgttc catggcaagg gcgtccaggt gatcgccgac atcgtcatca accaccgcac
2941 ggcggagcac aaggacggcc gcggcatcta ctgcctcttc gagggcggga cgcccgactc
3001 ccgcctcgac tggggcccgc acatgatctg ccgcgacgac ccctacggcg atggcaccgg
3061 caacccggac accggcgccg acttcgccgc cgcgccggac atcgaccacc tcaacaagcg
3121 cgtccagcgg gagctcattg gctggctcga ctggctcaag atggacatcg gcttcgacgc
3181 gtggcgcctc gacttcgcca agggctactc cgccgacatg gcaaagatct acatcgacgc
3241 caccgagccg agcttcgccg tggccgagat atggacgtcc atggcgaacg gcggggacgg
3301 caagccgaac tacgaccaga acgcgcaccg gcaggagctg gtcaactggg tcgatcgtgt
3361 cggcggcgcc aacagcaacg gcacggcgtt cgacttcacc accaagggca tcctcaacgt
3421 cgccgtggag ggcgagctgt ggcgcctccg cggcgaggac ggcaaggcgc ccggcatgat
3481 cgggtggtgg ccggccaagg cgacgacctt cgtcgacaac cacgacaccg gctcgacgca
3541 gcacctgtgg ccgttcccct ccgacaaggt catgcagggc tacgcataca tcctcaccca
3601 ccccggcaac ccatgcatcg tgagtagcca actcgatcag aaattctgaa tcatcctgca
3661 aactgatcga tgaactgatg ataaattctg taaaattgtt cagttctacg accatttctt
3721 cgattggggt ctcaaggagg agatcgagcg cctggtgtca atcagaaacc ggcaggggat
3781 ccacccggcg agcgagctgc gcatcatgga agctgacagc gatctctacc tcgcggagat
3841 cgatggcaag gtgatcacaa agattggacc aagatacgac gtcgaacacc tcatccccga
3901 aggcttccag gtcgtcgcgc acggtgatgg ctacgcaatc tgggagaaaa tctgagcgca
3961 cgatgacgag actctcagtt tagcagattt aacctgcgat ttttaccctg accggtatac
4021 gtatatacgt gccggcaacg agctgtatcc gatccgaatt acggatgcaa ttgtccacga
4081 agtacttcct ccgtaaataa agtaggatca gggacataca tttgtatggt tttacgaata
4141 atgctatgca ataaaatttg cactgcttaa tgcttatgca tttttgcttg gttcgattgt
4201 actggtgaat tattgttact gttcttttta cttctcgagt ggcagtattg ttcttctacg
4261 aaaatttgat gcgtag
//