GenBank-Updates@genbank.bio.net (05/22/91)
LOCUS VIBTRPOP 7419 bp ds-DNA BCT 22-MAY-1991
DEFINITION V. parahaemolyticus tryptophan operon containing a leader peptide
gene, trpE gene for anthranilate synthase alpha subunit (also
called component 1), trpG gene for anthranilate sythase beta
subunit, trpD gene for anthranilate phosphoribosyltransferase
ACCESSION X17149
KEYWORDS TrpE gene; anthranilate phosphoribosyltransferase;
anthranilate synthase; anthranilate synthase alpha subunit;
anthranilate synthase beta subunit; indo-3-glycerol phosphate;
indoleglycerol phosphate; operon;
phosphoribosylanthranilate isomerase; trpA gene; trpB gene;
trpC gene; trpC(F) gene; trpD gene; trpF gene; trpG gene;
tryptophan synthase; tryptophan synthase alpha subunit;
tryptophan synthase beta subunit.
SOURCE Vibrio parahaemolyticus DNA.
ORGANISM Vibrio parahaemolyticus
Prokaryota; Bacteria; Gracilicutes; Scotobacteria;
Facultatively anaerobic rods; Vibrionaceae.
REFERENCE 1 (bases 1 to 7419)
AUTHORS Crawford,I.P., Han,C.Y. and Silverman,M.
JOURNAL Unpublished (1989)
STANDARD full automatic
REFERENCE 2 (bases 1 to 7419)
AUTHORS Crawford,I.P., Han,C.Y. and Silverman,M.
TITLE Sequence and features of the tryptophan operon of Vibrio
parahemolyticus
JOURNAL DNA Sequence 1, 189-196 (1991)
STANDARD full automatic
COMMENT In V. paraheomolyticus the trpC and trpF genes are fused together.
From EMBL 26 entry VPTRPOP; dated 19-MAR-1991.
FEATURES Location/Qualifiers
prim_transcript 190..>7419
mat_peptide 220..342
/function="leader peptide"
CDS 220..345
/function="leader peptide"
/codon_start=220
mat_peptide 473..2095
/EC_number="4.1.3.27"
/product="anthranilate synthase, alpha subunit"
/gene="trpE"
CDS 473..2098
/EC_number="4.1.3.27"
/product="anthranilate synthase; alpha subunit"
/gene="trpE"
/codon_start=473
mat_peptide 2104..2691
/EC_number="4.1.3.27"
/product="anthranilate synthase, beta subunit"
/gene="trpG"
CDS 2104..2694
/EC_number="4.1.3.27"
/product="anthranilate synthase; beta subunit"
/gene="trpG"
/codon_start=2104
mat_peptide 2714..3709
/EC_number="2.4.2.18"
/product="anthranilate phosphoribosyltransferase"
/gene="trpD"
CDS 2714..3712
/EC_number="2.4.2.18"
/product="anthranilate phosphoribosyltransferase"
/gene="trpD"
/codon_start=2714
mat_peptide 3720..5161
/EC_number="4.1.1.48"
/note="trpC and TrpF are fused"
/product="phosphoribosylanthranilate isomerase (trpF)"
/gene="trpC(F)"
CDS 3720..5165
/EC_number="4.1.1.48"
/note="trpC and TrpF are fused"
/product="phosphoribosylanthranilate isomerase (trpF)"
/gene="trpC(F)"
/codon_start=3720
mat_peptide 5206..6393
/EC_number="4.2.1.20"
/product="tryptophan synthase; beta subunit"
/gene="trpB"
CDS 5206..6396
/EC_number="4.2.1.20"
/product="tryptophan synthase; beta subunit"
/gene="trpB"
/codon_start=5206
mat_peptide 6396..7199
/EC_number="4.2.1.20"
/product="tryptophan synthase; alpha subunit"
/gene="trpA"
CDS 6396..7202
/EC_number="4.2.1.20"
/product="tryptophan synthase; alpha subunit"
/gene="trpA"
/codon_start=6396
BASE COUNT 2139 a 1767 c 1729 g 1784 t
ORIGIN
1 caaccgtgtc gtgatctgta atcgccaaaa cctcgatatc aaaacccagt gccctatcca
61 ctaaatcttt cggttctaat cgaccatcgg acgccgtggt gtgactatgt aaatcaattc
121 tcatattttt gtcgttttaa tgctgttttg gggttgactt tttactctcg cactagttaa
181 ctagtacaca aatacaaagc acgtaattta aggctcacta tgttacaaga atttaaccag
241 aaccaaaaag cgaaagttgc tgtttgtctt aataagacaa actcagcaga tcttgcttgg
301 tggcgcactt ggacaagttc ttggtgggct aacgtgtact tctagtttag aaaaacaaac
361 gtcataaagc ccgctactca agcgggcttt ttattttgtc gcccaactca cattcaactg
421 cctaaacatt aatcggatat gaccaactct tgattgggag aacgtttgct ttatgcgact
481 atgtactgca ggacgattaa aacagctaca aggaggtctt gtgaacaagg ccattgaaat
541 taagaaactt ggacaactgg aagtattaaa agcttctgtt ccttacactc aagacccaac
601 ccgtctgttc catactattt gtgagaacaa aacggatagc ttgcttttag aatcggctga
661 aattgattct aaacagaatc tcaaatcgct gttaatcgtc gattctgcgg ttcgcatcgt
721 ttgttatgga cataccgtca gctttcatgc tctaacggag aacggcaaaa acctactgac
781 gcatttgaat caaaacgtac gtggcgaagt cgcaagccaa tttgacggcg aaacactgac
841 tttagaattc atccaaccat gcgatacgat tgatgaagat tctcgtctac gagaagcctc
901 ttcttttgat gcgcttcgtt tggtacagca cagttttgat ctttcttcgc aagacaaaca
961 tgccattttc cttggtggcc tatttgcgta tgacttggtg gcgaacttcg aaccattagg
1021 agacgcggta gcaacaaacc agtgtccgga ttacgtcttc tatgttgcgg aaacattact
1081 ggttgtagat caccaaacag aatcctgcca actgcaagca acattgttcg ttagcggctc
1141 gcaaaaagca gcgctagaaa gtcgaatcgc agatatccga gctcaatgca catcgccaaa
1201 gcgtcttcca gacgcaacgc aagtggccaa tatcacagct caaccaagcg tttcggatca
1261 agacttctgt caaatcgtcc gtgatctaaa agaattcgtg gtaaaaggcg atattttcca
1321 agtagttcct tctcgtcgat tcacattgcc ttgcccttct ccgctagcag cttacaaaga
1381 gctaaagcaa agcaacccaa gcccttacat gttctacatg caagatgagc tgttcacttt
1441 gtttggtgcg tctccagaaa gtgccttgaa gtatgaaacc gacaccaacc aaatagaaat
1501 ctacccaatt gcggggactc gccgtcgcgg taaacgtcca aatggcgaaa tcgattttga
1561 tttagatggc cgtattgaac ttgaactacg tagcgacaaa aaagagaacg ctgaacatat
1621 gatgttggta gacctagctc gtaatgatgt agcgcgcatc tcgcaagctg gtactcgtca
1681 tgttgccgat ctccttaaag tcgaccgcta tagccatgtt atgcacttgg tgtcccgcgt
1741 ggttggccag ttgcgcgatg accttgatgc tttgcacgct taccaagctt gtatgaacat
1801 gggcacgtta actggagcgc caaaaattcg tgcaatgcaa ctgattcgcg atgtcgaagg
1861 tgctcgtcgt ggcagctatg gcggagcggt gggctacctg acaggagaag gcacgctcga
1921 cacttgtatc gtgattcgct tggcttacgt ggaaaacggc attgctcaag tacaagcagg
1981 tgcaggcgtg gtattcgatt ctgaccctca agcggaagcc gatgaaacac gcggaaaagc
2041 tcaagcggtt atctctgcta ttcaagcagc gcattctcaa cctgcgaaca aggagtagca
2101 gacatggcga acatcgtatt catcgataac tttgactcgt tcacctataa ccttgtggac
2161 caattccgct cattaggtca ctcagtcaaa atttatcgta accacattcc ggcagaaacc
2221 atcgaacaag caataaacga gctcgaaaac cccgtcgttc tgctatcacc ggggccaggt
2281 gcgccatcag aagctggttc gatgccagag ctgattcaac gaatgaaagg caaagtgcca
2341 atgattggta tttgtcttgg tcaccaagcg attgttgaag cgtacggcgg tactgtcgct
2401 ggcgctggtg aaatcattca cggcaaagtg tcgatgatgg agcaccaaga ccacgcaatt
2461 tatcaaaatc tgccatcgcc acttgcaatt gcgcgttacc attctttagt ggcgacaaag
2521 gtaccagaca gcctgaccat cactgccgaa gtagacaatt tggtcatgtc tgttgtgcac
2581 gaacaagata aagtttgcgg atttcagttc cacccagagt caataatgac gacttacggc
2641 gcaaccttgt tgggcaacgc gattgaatgg gcgcttgaga aaaataacgc ataaggaaat
2701 ggggagagaa actatggaag caatcatcaa caaactgtac gaacagcaat cactgactca
2761 agaagaaagc caacagctat tcgacattat tattcgcggc gagcttgatc caattttaat
2821 ggcatcggca ttaacagcat taaagatcaa aggcgaaacg cctgatgaaa ttgccggtgc
2881 agcaaaagcc ctactggcga atgcgaaccc gttccctcgt cctgattacg actttgcaga
2941 catcgtaggg acagggggcg atggtcacaa tactatcaat atctcgacga cggccgcctt
3001 tgttgcagca gcatgtggct taaaagtcgc gaaacacggc aaccgcagcg tatcaagtaa
3061 atccggctct tctgacttac tggactcgtt tggcatcaac ctagcaatga gtgctgaaga
3121 cacccgcaaa gcggttgacg acattggcgt tgccttcttg ttcgcgcctc aatatcacgg
3181 aggcgttcgc cacgcgatgc ctgtgcgcca aactatgaaa acgcgcacca ttttcaacat
3241 tcttggcccg ttgataaatc ctgcacgtcc aaacatcgaa ctaatgggcg tctacagtga
3301 agaactggtt cgtcctatcg cagaaaccat gctacaaatg ggtatgaagc gcgccgctgt
3361 cgtacatggt agcggattgg atgaagtggc cattcacggt acaacaacgg ttgccgaaat
3421 caaagatggc aagatcactg aatacaccct aacgccagaa gattttggtc tagagtctca
3481 cccgctagaa gccattaaag gcggcgaccc ggaagaaaac aaagcaataa tcaccaacat
3541 tctgacgggt aaaggtacag acgcacaatt aggcgcagtc gcggttaacg tcgcacttct
3601 aatgcgttta ttcggccacg aagatttaaa agccaacacg cagcaagcga tcgaggctat
3661 gaactcaggc aaagcttacc aattggtaca gcaacttgcc gcgcacgctt aaggaacaga
3721 tgaagatgac tgacttcaac acccagcaag caaacaactt atcagagcac gtctcaaaaa
3781 aagaagcaga aatggcggaa gtgcttgcca aaattgttcg cgataaatac caatgggtag
3841 cagagcgaaa agcatcacag catctgagca cttttcaatc ggatctattg ccgtcggatc
3901 gcagttttta tgatgcattg agtggcgata aaaccgtatt cattacagaa tgcaaaaagg
3961 catcgccatc aaaaggcctt attcgcaacg acttcgattt ggactacatc gcgtcggttt
4021 acaacaacta cgcagacgcg atctcggttc tgaccgatga aaaatacttc cagggtagct
4081 tcgattttct acctcaagtt cgccgacaag tgaaacaacc agtgttgtgt aaggacttca
4141 tggtcgatac ctaccaagtt taccttgctc gtcattatgg cgcggatgcg gtattgctca
4201 tgctttcggt actgaatgac gaagagtaca aagcgttaga agaagctgcg cacagcctaa
4261 atatgggcat tttaacggaa gtcagcaacg aagaagagct tcaccgcgca gttcagcttg
4321 gcgctaaagt tatcggtata aacaaccgca acttgcgcga cttaacaacg gatttaaacc
4381 gaactaaagc gcttgcgcca accattcgta agcttgcacc aaacgcaacg gttatttcag
4441 agtctggcat ttacactcat caacaagttc gtgacttagc agaatatgca gacggctttt
4501 tgattggcag ctcgctgatg gcggaagaca acttagaact tgcggttcgt aaagtaacgc
4561 taggtgaaaa taaggtttgc ggtctgactc acccagatga tgcggcaaaa gcgtatcaag
4621 caggcgcagt atttggtggt ctgattttcg ttgagaaatc gaaacgagcg gtcgattttg
4681 aaagtgctcg cctaaccatg agcggcgcgc cacttaatta tgttggcgtg ttccaaaatc
4741 acgacgttga ttatgtcgcc tctatcgtaa cgtctttggg cttaaaagcc gtacagttac
4801 atgggctaga agatcaagag tacgtcaacc agctcaaaac agaactaccc gttggcgtcg
4861 agatttggaa agcctacggc gtggcggata caaagccatc cttgctcgca gacaacattg
4921 accgtcacct actggatgct caagttggta ctcaaactgg cggcacaggc cacgtgttcg
4981 actggtcttt gatcggcgat ccaagtcaaa tcatgctggc aggtggactg tcgcctgaaa
5041 atgcacagca agcagcaaaa ttagcctgct tgggattaga tttaaactct ggagtagaaa
5101 gcgcaccggg gaaaaaagat tcacagaagt tgcaagccgc atttcgcgct attcgtaact
5161 actaaagtta ttcgcagtta taaaaatacg agaaggatta tcaaaatggc aaaactgaat
5221 gcctactttg gcgaatatgg cggtcaatac gttccgcaaa ttctagttcc agcacttgag
5281 caactagagc aagcgtttat tgacgcgcaa gaagatccag aattccgcag cgagttcatg
5341 acgctccttc aggagtacgc tggtcgccca acggcactga ctctgactcg taacctaacc
5401 aaaggcacaa aaacaaaatt gtacctaaaa cgtgaagatc tgcttcacgg cggcgcacac
5461 aaaaccaacc aagtacttgg ccaggcgctg cttgcgaagc gcatgggtaa acacgaaatc
5521 atcgcagaaa caggcgcagg tcaacacggt gtagcaactg cacttgcgtg tgcactactt
5581 ggccttaagt gccgagttta catgggtgca aaagacgttg aacgtcaaag cccgaacgtg
5641 ttccgcatga aactgatggg tgcagaagta attcctgtcc attcaggctc agcaacactt
5701 aaagatgctt gtaatgaagc gctgcgtgat tggtcaggca gctacgaaga cgcacactac
5761 cttctcggta cagcagcagg ccctcaccca ttcccgacta ttgtgcgtga gttccaacgt
5821 atgattggtg aagaaactaa aaaccaaatt ctggcgcgtg aaggtcgcct acctgatgct
5881 gttatcgcct gtgtgggtgg tggctcaaac gcgattggta tgtttgcaga ttttatcgaa
5941 gaagaaagcg ttcgcctgat tggcgtggag ccagcaggta aaggcatcga tacagatcaa
6001 cacggtgcgc cacttaagca tggtaaaaca ggtatcttct tcggtatgaa ggcaccatta
6061 atgcaagatg agaatggtca ggttgaagag tcttactctg tttcagcagg tctagacttc
6121 ccttctgttg gtccacagca cgcacacctt aacgctatcg gtcgcgctga atacgacaac
6181 gtaacggatg atgaagcatt agaagcattc caagaactag ctcgcagcga aggcatcatt
6241 cctgcacttg aatcctccca tgctctggca catgcactgc gcatggctcg tgagaatcct
6301 gaaaaagagc aattactggt tgttaaccta tctggtcgag gtgacaaaga catcttcacc
6361 gtacacgcta tcttagaaga aaaaggagta atctaatgag ccgttatgag aagatgtttg
6421 cacgcctaaa cgagaaaaac caaggcgcat ttgtaccatt cgtgacggtt tgtgatccaa
6481 acgccgagca atcgtacaag atcatggaaa cactggttga atctggcgct gatgcgcttg
6541 aacttggtat tccgttctca gacccattgg cggacggccc aacaattcaa ggtgccaaca
6601 ttcgagcgtt agattcgggt gcaactccgg atatctgttt tgagcaaatc ggaaaaattc
6661 gagcaaagta cccagacctg ccaattggtc tattgatgta cgccaatctt gtatactcac
6721 gcggtataga gagcttctac gagcgctgtg caaaagcggg catcgattcc gttctcattg
6781 cagacgttcc gactaacgaa agcgcagagt tcgtagccgc ggcagagaaa tttggaatcc
6841 acccaatctt tatcgcaccc ccaacggcaa gtgacgagac gctaaaacaa gtttctgaat
6901 taggcggcgg ctacacttac ctgctttcgc gggcgggtgt gacgggtgca gaaaccaaag
6961 ctaacatgcc agttgaccac atgcttgaaa agctaaacca gtttgatgca ccaccagcgt
7021 tacttggttt cggcatttct gaaccagcac aagtaaaaca agccattgaa gcgggtgctg
7081 caggtgcaat ttcgggttca gcagtcgtaa aaatcatcga agcacacgta gagcaaccgc
7141 aaatcatgct cgataagctt ggtgaacttg tctcagcaat gaaagcggca actcaaaagt
7201 aatcgacacc acaagctaaa cactgcaaac gctcattgat tctcgttaat gggcgttttt
7261 ttatcaactt gattgcaaac ctcgcaatgc tctcacttcc aaccgtcttt taaagaagcg
7321 aatccataaa gtgataacat gccattgttc aaacctgttt tgtaaggtac tgacatggaa
7381 gatatcaaat cgttgttgct gcaatgccct gttttagcc
//