GenBank-Updates@genbank.bio.net (05/22/91)
LOCUS ECOKPSMT 1723 bp ds-DNA BCT 22-MAY-1991
DEFINITION E. coli kpsM and kpsT genes involved in polysaccharide transport
ACCESSION X53819
KEYWORDS kpsM gene; kpsT gene; membrane protein.
SOURCE Escherichia coli DNA.
ORGANISM Escherichia coli
Prokaryota; Bacteria; Gracilicutes; Scotobacteria;
Facultatively anaerobic rods; Enterobacteriaceae.
REFERENCE 1 (bases 1 to 1723)
AUTHORS SMITH,A.N.
JOURNAL Unpublished (1990)
STANDARD full automatic
REFERENCE 2 (bases 1 to 1723)
AUTHORS SMITH,A.N., Boulnois,G.J. and Roberts,I.S.
TITLE Molecular analysis of the Escherichia coli K5 kps locus:
identification and characterization of an inner-membrane capsular
polysaccharide transport system
JOURNAL Mol. Microbiol. 4, 1863-1869 (1990)
STANDARD full automatic
COMMENT Involved in polysaccharide transport across the inner membrane.
From EMBL 26 entry ECKPSMT; dated 25-MAR-1991.
FEATURES Location/Qualifiers
mRNA <177..>1624
/note="single transcriptional unit for kpsM and kpsT"
/gene="kpsT"
RBS 161..167
CDS 177..953
/product="kpsM product, predicted protein M(r)=29526"
/gene="kpsM"
/codon_start=177
RBS 931..937
CDS 950..1624
/product="kpsT product, predicted protein M(r)=24452"
/gene="kpsT"
/codon_start=950
BASE COUNT 483 a 315 c 387 g 538 t
ORIGIN
1 agatctattt atccctgcgg aaataatttc tgctgaaatt attttgcgac tattaaaaag
61 gtcaaaccgt ctgagtgaat tttatccagt tacaaataag cattacctcc agtgtattgg
121 tagctgttaa gccaagggcg gtagcgtacc tgaagagatt aggatcacat catcaaatgg
181 caagaagtgg atttgaagtc cagaaagtca ccgtagaggc attatttcta cgagaaatac
241 gaacacgctt tggtaagttc cgtctgggat atttgtgggc gattctcgaa ccctctgcgc
301 atttgctgat actgttgggc atttttggtt acattatgca ccgcacgatg ccagacatct
361 cattcccggt gtttttactt aatggcctga ttcccttttt tatctttagc agtatcagca
421 atcgttctgt aggcgctatt gaagcgaacc aggggttgtt taattatcga ccagtaaaac
481 ccatcgatac gatcattgca cgcgcactgc ttgagacgct gatttacgtt gctgtttata
541 tattgctcat gcttatcgtc tggatggcag gtgaatattt cgagataaca aactttttac
601 aactagtgct cacctggagt ttgttaatca ttctttcatg tggcatcggc ttaatattca
661 tggtcgttgg taaaaccttc cctgaaatgc aaaaggtcct gccgatactg cttaagccgc
721 tgtatttcat ctcctgcatc atgttccctc tacattcgat tccgaagcaa tactggtcat
781 atctactctg gaacccatta gtgcatgtcg tagagttaag ccgcgaggca gttatgcctg
841 gctatatcag cgaaggcgtg agtctgaact accttgcaat gttcactctg gtcaccctgt
901 tcatcggtct ggcgttatac cgaactcgtg aggaggcaat gctgacatca tgattaagat
961 tgagaatttg acgaagtcat atcgtacgcc aacggggcga cactatgtgt ttaaggattt
1021 aaacatcgaa atcccttcag gaaaaagcgt tgcctttatt ggtcgtaatg gtgcgggtaa
1081 atcaacgtta ctgagaatga ttggtggcat tgaccgcccc gatagcggaa agatcatcac
1141 caataaaacg atatcatggc cagtcggcct cgccggtgga ttccagggaa gtttaaccgg
1201 acgcgaaaac gtaaaatttg ttgcgcggtt atacgcgaag caagaagagc tgaaagagaa
1261 aattgagttt gtcgaagaat ttgccgaact cggtaagtac tttgatatgc cgatcaaaac
1321 ttactcctca ggtatgcgat cgcgtttagg ctttggttta agcatggcat ttaagtttga
1381 ttattatatc gtcgatgaag taacagcggt cggtgatgcc aggtttaaag agaagtgcgc
1441 tcaattgttt aaagaaaggc ataaagagtc tagctttttg atggtttcac atagtttgaa
1501 ttcattgaaa gagttttgtg atgtggcgat tgtttttaaa aatagctata ttattggtta
1561 ttatgagaat gttcaaagtg gtatagatga gtataaaatg tatcaagatt tagatattga
1621 atgataatta tattaaataa ttaactctta gttcaggtat ttatatgttc tgcttgtcat
1681 tcgttggtgt taataataaa attaatatga caaatagcat atg
//