GenBank-Updates@genbank.bio.net (05/22/91)
LOCUS ECOPBPBRR 1500 bp ds-DNA BCT 22-MAY-1991
DEFINITION E.coli pbpB gene regulatory region
ACCESSION X52063
KEYWORDS binding protein; pbpB gene; penicillin-binding protein;
penicillin-binding protein 3.
SOURCE Escherichia coli DNA.
ORGANISM Escherichia coli
Prokaryota; Bacteria; Gracilicutes; Scotobacteria;
Facultatively anaerobic rods; Enterobacteriaceae.
REFERENCE 1 (bases 1233 to 1500)
AUTHORS Nakamura,M., Maruyama,I.N., Soma,M., Kato,J., Suzuki,H. and
Hirota,Y.
TITLE On the process of cellular division in Escherichia coli: Nucleotide
sequence of the gene for penicillin-binding protein 3
JOURNAL Mol. Gen. Genet. 191, 1-9 (1983)
STANDARD full automatic
REFERENCE 2 (bases 1 to 1232)
AUTHORS Ayala,J.A.
JOURNAL Unpublished (1990)
STANDARD full automatic
REFERENCE 3 (bases 1 to 1500)
AUTHORS Gomez,M.J., Fluoret,B., Van,H.J. and Ayala,J.A.
TITLE Nucleotide sequence of the regulatory region of pbpB gene of
Escherichia coli
JOURNAL Nucleic Acids Res. 18, 2813-2813 (1990)
STANDARD full automatic
COMMENT SWISS-PROT; P18595; YPBB$ECOLI.
*source: strain=K12, W3110; library=plasmid, Clarke and Carbon;
*source: clone=pLC26-6; **map: map position=2 minutes on E.coli
map; See <K00137> for E.coli pbpB gene. Data kindly reviewed
(16-JUL-1990) by J.A. Ayala
From EMBL 26 entry ECPBPBRR; dated 26-MAR-1991.
FEATURES Location/Qualifiers
CDS 77..535
/note="orfC"
/codon_start=77
misc_feature 303..324
/note="(pot.) SOS box I"
misc_feature 366..389
/note="(pot.) SOS box II"
misc_feature 405..417
/note="-35 region (pot.)"
misc_feature 412..434
/note="(pot.) stem loop structure"
misc_feature 434..439
/note="-10 region (pot.)"
mRNA 453..>1475
/note="orfB mRNA"
RBS 464..469
/note="ribosome binding site (pot.)"
RBS 519..522
/note="putative"
CDS 537..1475
/note="orfB"
/codon_start=537
terminator 803..822
/note="(pot.) transcription terminator"
misc_feature 815..843
/note="(pot.) stem loop structure"
misc_feature 1447..1467
/note="(pot.) SOS box III"
mRNA 1455..>1500
/note="orfA mRNA"
CDS 1475..>1500
/note="orfA"
/codon_start=1475
BASE COUNT 357 a 377 c 432 g 334 t
ORIGIN
1 aagcttttcc tcagctccgt aaactccttt cagtgggaaa ttgtggggca aagtgggaat
61 aaggggtgag gctggcatgt tccggggagc aacgttagtc aatctcgaca gcaaagggcg
121 cttatcagtg cctacccgtt atcgggaaca gctgcttgag aacgctgccg gtcaaatggt
181 ttgcaccatt gacatttatc acccgtgcct gctgctttac cccctgcctg aatgggaaat
241 tatcgagcaa aaattatcgc gtctgtcgag catgaacccg gttgagcgcc gtgtgcagcg
301 cctactgtta ggtcatgcca gcgaatgtca gatggatggc gcaggtcgat tgttaatcgc
361 gccagtactg cggcaacatg ccgggctgac aaaagaagtg atgctggttg gacagttcaa
421 caagtttgag ctgtgggatg aaacaacctg gcatcaacag gtcaaggaag atatcgacgc
481 agagcagttg gctaccggag acttatcgga gcgactgcag gacttgtctc tataaaatga
541 tggaaaacta taaacatact acggtgctgc tggatgaagc cgttaatggc ctcaatatcc
601 gtcctgatgg catctacatt gatgggactt ttggtcgcgg tggtcactca cgtctgatcc
661 tctcgcagct tggcgaagag gggcgtttgc tggcgatcga tcgcgacccg caggctatcg
721 ccgttgcgaa gactattgat gatccgcgct tctccatcat ccacggacct ttctccgcgc
781 tgggcgaata cgttgccgag cgcgatctta tcggcaagat cgacggcatt ctcctcgatc
841 ttggcgtctc ttcaccgcaa cttgatgatg ctgaacgtgg cttttccttt atgcgcgatg
901 gtccgctgga catgcgtatg gacccaaccc gtgggcagtc agccgctgaa tggctacaaa
961 ccgcagaaga agccgatatc gcctgggtat tgaaaaccta tggtgaagag cgttttgcca
1021 aacgcattgc ccgcgccatt gtcgagcgta accgcgaaca gccgatgacc cgcaccaaag
1081 aactggcgga agtcgtggct gctgcaacgc cggtgaaaga taagtttaaa catcccgcga
1141 cccgtacctt ccaggcggtg cgcatttggg taaacagtga actggaggag atagagcagg
1201 cgctaaaaag ctcgctcaac gtgctggccc cgggtgggcg gctttcgatc atcagcttcc
1261 actcgctgga agaccgtatt gtgaaacgtt ttatgcgtga aaacagccgc ggtccgcaag
1321 ttccggcagg gttaccgatg actgaagagc agctcaaaaa actgggtggc cgtcagctgc
1381 gagcactagg caagttaatg ccgggcgaag aagaggtggc tgagaaccct cgtgcccgta
1441 gttcagttct gcgtattgca gagaggacga atgcatgatc agcagagtga cagaagctct
//