GenBank-Updates@genbank.bio.net (05/22/91)
LOCUS LACTLE 1296 bp ds-DNA BCT 22-MAY-1991
DEFINITION Lactococcus lactis DNA for the transposon-like element on the
lactose plasmid
ACCESSION X53013
KEYWORDS transposase; transposon-like element; unidentified reading frame.
SOURCE Lactococcus lactis DNA.
ORGANISM Lactococcus lactis
Prokaryota; Bacteria; Firmicutes; Regular asporogenous rods;
Lactobacillaceae.
REFERENCE 1 (bases 1 to 1296)
AUTHORS Dao,C.H.
JOURNAL Unpublished (1990)
STANDARD full automatic
REFERENCE 2 (bases 1 to 1296)
AUTHORS Huang,D.C., Novel,M. and Novel,G.
TITLE A transposon-like element on the lactose plasmid of Lactococcus
lactis subsp. lactis Z270
JOURNAL FEMS Microbiol. Lett. 77, 101-106 (1991)
STANDARD full automatic
COMMENT *source: strain=z270; clone=D412;
From EMBL 26 entry LLTLE; dated 26-MAR-1991.
FEATURES Location/Qualifiers
promoter 31..36
/note="-35 region"
promoter 54..59
/note="-10 region"
CDS complement(78..206)
/note="ORF5 (AA 1 - 43)"
/codon_start=206
RBS 89..94
/note="ribosome binding site"
CDS 102..1253
/note="ORF1 (AA 1 - 384)"
/codon_start=102
CDS complement(537..701)
/note="ORF4 (AA 1 - 55)"
/codon_start=701
CDS complement(1104..1205)
/note="ORF3 (AA 1 - 34)"
/codon_start=1205
CDS 1120..1263
/note="ORF2 (AA 1 - 48)"
/codon_start=1120
BASE COUNT 445 a 222 c 267 g 362 t
ORIGIN
1 tggaaaggtt ataataaact agacacaaag ttaagagaaa tcgttggaaa ggttataata
61 aactagacac aaagttaaga gaaatcgtgg aaaggtttat tatggcaaga agaaaattcg
121 ataaacaatt taaaaattct gcagtaaaac tcattcttga agagggttac tctgttaaag
181 aagtcagcca agagcttgag gttcatgcca atagtcttta tcgctgggtt caagaagttg
241 aagaatatgg agaaagtgct tttccaggca atgggacagc cctagctgat gcccaacata
301 agattaaatt gttagagaaa gaaaatcgtt atcttcagga ggaacttgaa cttctaaaaa
361 agttccgggt ccttcttgaa gcgaacagag taaaacgttt tgaatttctc ttgaaacatc
421 atgggaagat aaaaattaag catgcagtaa aagttcttaa ggtttctcgc tcaggtttct
481 atgaatacat gcatcgtcgt ccttcaaaac aacaagtgga gagagaaatt ctctcagaga
541 agataaaagc tgtctttcat gagcataagg gacgctatgg tgcggttaga attaccaagg
601 tacttcataa tactggtatt atgaccaaca cgaaacgtgt tgggaaactg atgcacttga
661 tgggacttta tgccaaggga agccgttata aatataaaca ttacaacaga aaaaaagggg
721 aacgttcgct ttcaagaccc aatttaatta atcagatctt taaagcaaca gctcctaata
781 aagtatggct gggagacatg acctatatcc ctaccaaaga aggcacctta tacttagccg
841 tgaatatcga cgttttttca cgtaagattg taggctggtc aatgtcttca cggatgcaag
901 ataaactggt gagggattgc ttcttacaag cttgtgggaa agaacatcct cagcctggct
961 tgattgtcca tactgatcaa gggagtcaat ataccagctc tcgttatcaa tctactcttc
1021 gtcaagtcgg tgctcaatct agcatgagtc gtaaaggaaa tccctatgac aatgcaatga
1081 tggagtcttt ttataagacg ctaaagagag agcttattaa tgatgctcat tttgagacaa
1141 gagctgaggc tactcaagaa atatttaaat acattgagac ctattacaat acaaaatgga
1201 tgcattcagg tcttgattac cagtcgccaa aagactttga aaaatattat tcttaaattc
1261 tcttaactcc gtgtctagtt tttcgttgac tttcca
//