GenBank-Updates@genbank.bio.net (05/22/91)
LOCUS THACITSYN 1520 bp ds-DNA BCT 22-MAY-1991
DEFINITION T. acidophilum gene for citrate synthase. (EC 4.1.3.7)
ACCESSION X55282
KEYWORDS citrate synthase.
SOURCE Thermoplasma acidophilum DNA.
ORGANISM Thermoplasma acidophilum
Prokaryota; Bacteria; Mendosicutes; Archaeobacteria;
Thermoplasmales.
REFERENCE 1 (bases 1 to 1520)
AUTHORS Sutherland,K.J.
JOURNAL Unpublished (1990)
STANDARD full automatic
REFERENCE 2 (bases 1 to 1520)
AUTHORS Sutherland,K.J., Henneke,C.M., Towner,P., Hough,D.W. and
Danson,M.J.
TITLE Citrate synthase from the thermophilic archaebacterium Thermoplasma
acidophilum. Cloning and sequencing of the gene
JOURNAL Eur. J. Biochem. 194, 839-844 (1990)
STANDARD full automatic
COMMENT *source: strain=DSM 172;
From EMBL 26 entry TACITSYN; dated 26-MAR-1991.
FEATURES Location/Qualifiers
promoter 166..171
/note="archaebacterial promoter box A"
promoter 194..197
/note="archaebacterial promoter box B"
RBS 259..264
mat_peptide 270..1424
/product="citrate synthase"
CDS 270..1427
/product="citrate synthase"
/codon_start=270
BASE COUNT 452 a 329 c 379 g 360 t
ORIGIN
1 cgctccatat cggcgtcgct tcgatatttc atcataacta tattaccgct tttgtttctc
61 atcacgttat tcctctggat tgttctacgg ctttggcata ttaatcttat tctccgcacg
121 gatcggactt cgctattctt gccgattggg tctaaatagt atatttttaa tctatttatt
181 tgaatattaa aatatgtata ccgatcccaa aattatacga cgaaatttca aatagcgtta
241 aaagatatca tatatgtaga ggtgtattaa tgccagaaac tgaagaaatt agcaaagggc
301 ttgaggatgt caatataaag tggacaaggc tcacgaccat cgacggaaat aagggaatcc
361 tgcgatacgg gggctattct gtcgaagata taattgcctc tggggcacag gatgaggaga
421 tccagtatct gttcctgtat gggaaccttc caactgaaca ggagctcaga aaatacaagg
481 aaaccgtcca gaagggttat aagataccag attttgtcat aaacgccata aggcagctgc
541 caagggaatc ggatgctgtg gcaatgcaga tggccgcggt tgcagccatg gcagcatcgg
601 agacaaagtt caagtggaac aaggatacgg acagagacgt tgcagcagaa atgatcggca
661 ggatgtcagc gataaccgtg aacgtgtaca ggcacataat gaacatgccg gccgagcttc
721 cgaaaccatc agacagctac gctgagagct tcctgaacgc tgcgtttgga aggaaggcaa
781 caaaggaaga gatagacgcc atgaatacgg cactgattct ctacacagac catgaggtgc
841 ctgcatctac cacggcaggc ttggtcgcgg tatcaaccct ctcagacatg tactccggca
901 taactgcagc tcttgcggcc ctcaagggtc cgctgcatgg cggtgcagct gaggcagcaa
961 tagcgcagtt cgatgagata aaggatcccg ccatggtgga gaagtggttc aacgacaaca
1021 taataaacgg aaagaagaga ctcatgggct tcggccacag ggtatacaag acctacgatc
1081 caagggcaaa gatattcaag ggcatcgctg agaaactctc cagcaagaag ccagaggtgc
1141 acaaggtata tgagatcgcc acgaagcttg aagactttgg catcaaggca ttcggttcaa
1201 agggcatata tccgaacacg gattacttct ccggaatagt atacatgtcc ataggattcc
1261 cgctgaggaa caacatatac acggcgctct tcgcgctatc cagggttaca gggtggcagg
1321 cacacttcat agagtacgtg gaggaacagc agagactgat taggccgaga gccgtttacg
1381 tcggtcctgc agagagaaag tacgtaccca tcgctgaaag aaagtgaaaa cacttttctc
1441 aaatttttat taatatattt tatatacaac ggcgcttgtt gtggaaattt cgattagcga
1501 tggtctgtgt tttcggatcc
//