GenBank-Updates@genbank.bio.net (05/22/91)
LOCUS VFALEA1 1585 bp ss-mRNA PLN 22-MAY-1991 DEFINITION Vicia faba mRNA for Legumin A1 pre-pro-polypeptide ACCESSION X55013 KEYWORDS legumin A; storage protein. SOURCE Vicia faba RNA. ORGANISM Vicia faba Eukaryota; Plantae; Embryobionta; Magnoliophyta; Magnoliopsida; Rosidae; Rosales; Fabaceaea. REFERENCE 1 (sites) AUTHORS Bassuener,R., Wobus,U. and Rapoport,T.A. TITLE Signal recognition particle triggers the translocation of storage globulin polypeptides from field beans (Vicia faba L.) across mammalian endoplasmic reticulum membrane. JOURNAL FEBS Lett. 166, 314-320 (1984) STANDARD full staff_review REFERENCE 2 (sites) AUTHORS Horstmann,C. TITLE New aspects of the subunit structure of Vicia faba legumin. JOURNAL Kulturpflanze 32, 109-116 (1984) STANDARD full staff_review REFERENCE 3 (bases 1 to 1585) AUTHORS Bassuener,R. JOURNAL Unpublished (1990) STANDARD full automatic REFERENCE 4 (bases 1 to 1585) AUTHORS Schlesier,B., Bassuener,R., Nong,V.H. and Muentz,K. TITLE The cDNA derived primary structure of two distinct legumin A subunit precursors from field bean (Vicia faba L.) JOURNAL Nucleic Acids Res. 18, 7146-7146 (1990) STANDARD full staff_review COMMENT *source: strain=var. minor cv.Fribo clone=Vfle 165 Author's comment:For legumin A encoding cDNA's of Vicia faba see: Wobus et al.: FEBS Lett. 201:74-80(1986) and x55014. For legumin B encoding genes of Vicia faba see: X03677; X14237; X14238;X14239; X14240; X14241." "The classification is according to Nielsen et al.: The Plant Cell 1:313-328 (1989), as compared to other legumin-like storage globulin subunits." From EMBL 26 entry VFLEA1; dated 05-APR-1991. FEATURES Location/Qualifiers mRNA <1..>1585 /partial /gene="LeA1" /citation=[4] /citation=[1] mat_peptide <1..926 /codon_start=3 /note="The region encoding nine amino acids following the N terminus of this chain are not present in the reported cDNA." /product="legumin A1 acidic alpha chain (N-terminal incomplete)" /gene="LeA1" /citation=[4] /citation=[2] CDS <1..1496 /partial /codon_start=3 /note="The region encoding the signal peptide and following nine amino acids are not present in the reported cDNA." /product="N-terminal incomplete legumin A1 pre-pro-polypeptide" /gene="LeA1" /codon_start=1 /citation=[4] /citation=[1] /citation=[2] misc_feature 246..284 /note="hydrophilic segment" misc_feature 465..602 /note="see: Nielsen et al.: The Plant Cell 1:313-328(1989). For legumin A1 this variable region is organized as a 69bp tandem repeat: from 465 to 633, and from 634 to 602, never described before." misc_feature 753..923 /note="hydrophilic segment" repeat_region 792..815 /rpt_type=tandem /rpt_unit=792..797 mat_peptide 927..1493 /note="The legumin mature subunit consists of one acidic alpha and one basic beta chain linked by a disulfide bridge, and generated from the described pro-polypeptide precursor." /product="legumin A1 basic beta chain" /gene="LeA1" /citation=[4] /citation=[2] repeat_region 1437..1498 /rpt_type=tandem /rpt_unit=1437..1467 /note="This 31bp tandem repeat unit is described for the first time. It is unusual as compared to other repetetive structures in legumin cDNA since it causes a shift in the reading frame (see legumin A2)." BASE COUNT 522 a 337 c 379 g 347 t ORIGIN 1 agtgtcagct agaacgcctc gatgcccttg aaccagataa ccgtatagag tcagaaggtg 61 gactcattga gacttggaat ccaaacaaca ggcaattccg atgtgcaaga gtggcactct 121 ctcgtgctac ccttcaacgc aatgcccttc gcagacctta ctattccaat gctccccaag 181 aaatttacat ccaacaaggt aatggatatt ttggcatggt attccctagt tgtcctgaga 241 cctttgagga gccacaacaa tctgagcaag gagaaggacg caggtacaga gacagccacc 301 aaaaggttaa cagattcaga cagggtgata tcattgcagt tcctactggt attgtatttt 361 ggatgtacaa tgaccaagat attccagtta ttgccatttc tcttactgat actggcagct 421 ccaataacca gcttgatcag atgcctagga gattctatct tgctgggaat caagagcaag 481 agtttctacg ataccaacat caacaaggag taaaggaaga acaagacaat gacgggaacc 541 aagagcaaga gtttctacga taccaacatc gacaaggagt aaaggaagaa caagacaatg 601 acgggaacaa cattttcagc ggcttcaaca gggatttctt ggaagatgct ttcaacgtga 661 acaggcatat agtagacaga cttcaaggca ggaacgaaga gaggggagcc attgtcaaag 721 tgaaaggtgg tctcagcatc ataacccctc cggagaggca agcgcgccac ccgagaggca 781 gcagacaaga agaagatgaa gatgaagatg aagatgaaaa ggaagagaga caaccaagcc 841 atcataaaag cagaagaggt gaagatgaag acgacaagga aaagcgccac agccaaaaag 901 gtgaaagcag aaggcatgga gacaatgggc ttgaggaaac cgtttgcact gccaaacttc 961 ggctgaacat tggttcatct tcatcgccag acatctacaa ccctcaagct ggtagaatca 1021 aaactgttac cagcgttgac ctcccagttc tcaggtggct caaactaagt gctgagcatg 1081 gatctctccg caaaaatgct atgtttgtgc ctcactacaa cctcaacgca aacagcgtat 1141 tatacgcatt gaagggacgt gcaaggctac aagttgtgaa ctgcaatggc aacactgtgt 1201 ttgatggaga gctagaagcc ggtcgtgcat tgacagtgcc acaaaactat gttgtggctg 1261 caaaatcatt gagcgacagg ttcacatatg tagcattcaa gacaaatgac agagctggta 1321 ttgcaagact agcagggaca tcatcagtta taaatgatct gcctttggat gtagtcgcag 1381 ctacattcaa cttggagagg aatgaggcaa ggcagctcaa gttcaacaat ccttccagat 1441 ttctagttcc accacgcgag tctgagaaga tttctagttc caccacgcga gtatgagaag 1501 agagcttccg cttagatttg tcatcaaatc gatgaaaata ttaataatga ataagaaagg 1561 taaggcttaa atggctttgt tgttg //
GenBank-Updates@genbank.bio.net (05/22/91)
LOCUS VFALEA2 1666 bp ss-mRNA PLN 22-MAY-1991 DEFINITION Vicia faba mRNA for Legumin A1 pre-pro-polypeptide ACCESSION X55014 KEYWORDS legumin A; storage protein. SOURCE Vicia faba RNA. ORGANISM Vicia faba Eukaryota; Plantae; Embryobionta; Magnoliophyta; Magnoliopsida; Rosidae; Rosales; Fabaceaea. REFERENCE 1 (sites) AUTHORS Bassuener,R., Wobus,U. and Rapoport,T.A. TITLE Signal recognition particle triggers the translocation of storage globulin polypeptides from field beans (Vicia faba L.) across mammalian endoplasmic reticulum membrane. JOURNAL FEBS Lett. 166, 314-320 (1984) STANDARD full staff_review REFERENCE 2 (sites) AUTHORS Horstmann,C. TITLE New aspects of the subunit structure of Vicia faba legumin. JOURNAL Kulturpflanze 32, 109-116 (1984) STANDARD full staff_review REFERENCE 3 (bases 1 to 1666) AUTHORS Bassuener,R. JOURNAL Unpublished (1990) STANDARD full automatic REFERENCE 4 (bases 1 to 1666) AUTHORS Schlesier,B., Bassuener,R., Nong,V.H. and Muentz,K. TITLE The cDNA derived primary structure of two distinct legumin A subunit precursors from field bean (Vicia faba L.) JOURNAL Nucleic Acids Res. 18, 7146-7146 (1990) STANDARD full staff_review COMMENT *source: strain=var. minor cv.Fribo, clone= Vfle 129 author's comment:"The supposed site of polyadenylation is four bp downstream of the reported 3' terminal nucleotide." "For legumin A encoding cDNA's of Vicia faba see: Wobus et al.: FEBS Lett. 201: 74-80(1986) and X55013; for legumin B encoding genes of Vicia faba see: X03677; X14237; X14238; X14239; X14240; X14241." "The classification is according to Nielsen et al.: The Plant Cell 1: 313-328(1989), as compared to other legumin-like seed storage globulin subunits." From EMBL 26 entry VFLEA2; dated 05-APR-1991. FEATURES Location/Qualifiers mRNA <1..>1666 /partial /gene="LeA2" /citation=[4] /citation=[1] sig_peptide 24..86 /product="signal peptide for cotranslational translocation" CDS 24..1526 /product="legumin A2 primary translation product" /gene="LeA2" /codon_start=24 /citation=[4] /citation=[1] /citation=[2] mat_peptide 87..968 /note="The legumin mature subunit consists of one acidic alpha and one basic beta chain, linked by a disulfide bridge, and generated from the described pro-polypeptide precursor." /product="legumin A2 acidic alpha chain" /gene="LeA2" /citation=[4] /citation=[2] CDS 87..1526 /product="legumin A2 pro-polypeptide" /gene="LeA2" /codon_start=87 /citation=[4] /citation=[1] misc_feature 357..395 /note="hydrophilic segment" misc_feature 576..644 /note="hydrophilic segment" misc_feature 801..965 /note="hydrophilic segment" repeat_region 840..857 /rpt_type=tandem /rpt_unit=840..845 mat_peptide 969..1523 /note="The mature legumin subunit consists of one acidic alpha and one basic beta chain, linked by a disulfide bridge, and generated from the described pro-polypeptide precursor." /product="legumin A2 basic beta chain" /gene="LeA2" /citation=[4] /citation=[2] polyA_signal 1643..1648 BASE COUNT 537 a 359 c 385 g 385 t ORIGIN 1 acattctcta aatatctgtt atcatggcta aacttcttgc actctctctt tcactttgct 61 ttctactttt cagtagctgc tttgctctca gagaacagtc tcaacaaaat gagtgtcagc 121 tagaacgcct cgatgccctt gaaccagata accgtataga gtcagaaggt ggacttattg 181 agacttggaa tccaaacaac aggcaattcc gatgtgcaag cgtggccctc tctcgtgcta 241 cccttcagcg caatgccctt cgcagacctt actattccaa tgctccccaa gaaatttaca 301 tccaacaagg taatggatat tttggcatgg tattccctag ttgtcctgag acctttgagg 361 agccccaaca atctgaacaa ggagagggag gcaggtacag agacagccac caaaaggtta 421 accgatttag agagggtgat atcattgcag ttcctactgg tattgtattt tggatgtaca 481 atgaccaaga tactccagtt attgccattt ctcttactga tattggcagc tccaataacc 541 agcttgatca gatgcctagg agattctatc ttgctgggaa ccaagagcaa gagtttctac 601 gataccaaca tcaacaagga ggaaaggaag aacaagacaa tgacgggaac aacattttca 661 gcggcttcaa gagggatttc ttggaagatg cgttgaacgt gaacaggcat atagtagaca 721 gacttcaagg caggaacgaa gacgaagaga agggagccat tgtcaaagtg aaaggtggtc 781 tcagcatcat aacccctccg gagaggcaag cgcgccaccc gagaggcagc agacaagaag 841 aagatgaaga tgaagatgaa aaggaagaga gacaaccaag ccatcataaa agcagaagag 901 gtgaagatga agacgacaag gaaaagcgcc acagccaaaa aggtgaaagc agaaggcatg 961 gagacaatgg gcttgaggaa accgtttgca ctgccaaact tcggctgaac attggttcat 1021 cttcatcgcc agacatctac aaccctcaag ctggtagaat caaaactgtt accagccttg 1081 acctcccagt tctcaggtgg ctcaaactaa gtgctgagca tggatctctc cgtaaaaatg 1141 ctatgtttgt gcctcactac aacctcaacg caaacagcat attatacgca ttgaagggac 1201 gtgcaaggct acaagttgtg aactgcaatg gcaacactgt gtttgatgaa gagctagaag 1261 ccggtcgtgc attgacagtg ccacaaaact atgctgtggc tgcaaaatca ttgagcgaca 1321 ggttcacata tgtagcattc aagaccaatg atagagctgg tattgcaaga cttgcaggga 1381 catcatcagt tataaatgat atgccggtgg atgtggttgc agctacattc aacttggaga 1441 ggaatgaggc aaggcagctc aagtccaaca atcccttcaa atttctagtt ccaccgcgtg 1501 agtctcagaa gagagcttcc gcttagattt gtcatcaaat cgatgaaaat aataatgaaa 1561 ataatgaata agaaaactaa ggcttagatg cctatgttgt tggtgtaaaa taacacgagt 1621 catgtacctt ttgcggcaac agaataaaca aagaagcgaa gttcca //