GenBank-Updates@genbank.bio.net (05/22/91)
LOCUS VFALEA1 1585 bp ss-mRNA PLN 22-MAY-1991
DEFINITION Vicia faba mRNA for Legumin A1 pre-pro-polypeptide
ACCESSION X55013
KEYWORDS legumin A; storage protein.
SOURCE Vicia faba RNA.
ORGANISM Vicia faba
Eukaryota; Plantae; Embryobionta; Magnoliophyta; Magnoliopsida;
Rosidae; Rosales; Fabaceaea.
REFERENCE 1 (sites)
AUTHORS Bassuener,R., Wobus,U. and Rapoport,T.A.
TITLE Signal recognition particle triggers the translocation of storage
globulin polypeptides from field beans (Vicia faba L.) across
mammalian endoplasmic reticulum membrane.
JOURNAL FEBS Lett. 166, 314-320 (1984)
STANDARD full staff_review
REFERENCE 2 (sites)
AUTHORS Horstmann,C.
TITLE New aspects of the subunit structure of Vicia faba legumin.
JOURNAL Kulturpflanze 32, 109-116 (1984)
STANDARD full staff_review
REFERENCE 3 (bases 1 to 1585)
AUTHORS Bassuener,R.
JOURNAL Unpublished (1990)
STANDARD full automatic
REFERENCE 4 (bases 1 to 1585)
AUTHORS Schlesier,B., Bassuener,R., Nong,V.H. and Muentz,K.
TITLE The cDNA derived primary structure of two distinct legumin A
subunit precursors from field bean (Vicia faba L.)
JOURNAL Nucleic Acids Res. 18, 7146-7146 (1990)
STANDARD full staff_review
COMMENT *source: strain=var. minor cv.Fribo clone=Vfle 165
Author's comment:For legumin A encoding cDNA's of Vicia faba see:
Wobus et al.: FEBS Lett. 201:74-80(1986) and x55014. For legumin B
encoding genes of Vicia faba see: X03677; X14237; X14238;X14239;
X14240; X14241." "The classification is according to Nielsen et
al.: The Plant Cell 1:313-328 (1989), as compared to other
legumin-like storage globulin subunits."
From EMBL 26 entry VFLEA1; dated 05-APR-1991.
FEATURES Location/Qualifiers
mRNA <1..>1585
/partial
/gene="LeA1"
/citation=[4]
/citation=[1]
mat_peptide <1..926
/codon_start=3
/note="The region encoding nine amino acids following the
N terminus of this chain are not present in the reported
cDNA."
/product="legumin A1 acidic alpha chain (N-terminal
incomplete)"
/gene="LeA1"
/citation=[4]
/citation=[2]
CDS <1..1496
/partial
/codon_start=3
/note="The region encoding the signal peptide and
following nine amino acids are not present in the reported
cDNA."
/product="N-terminal incomplete legumin A1
pre-pro-polypeptide"
/gene="LeA1"
/codon_start=1
/citation=[4]
/citation=[1]
/citation=[2]
misc_feature 246..284
/note="hydrophilic segment"
misc_feature 465..602
/note="see: Nielsen et al.: The Plant Cell
1:313-328(1989). For legumin A1 this variable region is
organized as a 69bp tandem repeat: from 465 to 633, and
from 634 to 602, never described before."
misc_feature 753..923
/note="hydrophilic segment"
repeat_region 792..815
/rpt_type=tandem
/rpt_unit=792..797
mat_peptide 927..1493
/note="The legumin mature subunit consists of one acidic
alpha and one basic beta chain linked by a disulfide
bridge, and generated from the described pro-polypeptide
precursor."
/product="legumin A1 basic beta chain"
/gene="LeA1"
/citation=[4]
/citation=[2]
repeat_region 1437..1498
/rpt_type=tandem
/rpt_unit=1437..1467
/note="This 31bp tandem repeat unit is described for the
first time. It is unusual as compared to other repetetive
structures in legumin cDNA since it causes a shift in the
reading frame (see legumin A2)."
BASE COUNT 522 a 337 c 379 g 347 t
ORIGIN
1 agtgtcagct agaacgcctc gatgcccttg aaccagataa ccgtatagag tcagaaggtg
61 gactcattga gacttggaat ccaaacaaca ggcaattccg atgtgcaaga gtggcactct
121 ctcgtgctac ccttcaacgc aatgcccttc gcagacctta ctattccaat gctccccaag
181 aaatttacat ccaacaaggt aatggatatt ttggcatggt attccctagt tgtcctgaga
241 cctttgagga gccacaacaa tctgagcaag gagaaggacg caggtacaga gacagccacc
301 aaaaggttaa cagattcaga cagggtgata tcattgcagt tcctactggt attgtatttt
361 ggatgtacaa tgaccaagat attccagtta ttgccatttc tcttactgat actggcagct
421 ccaataacca gcttgatcag atgcctagga gattctatct tgctgggaat caagagcaag
481 agtttctacg ataccaacat caacaaggag taaaggaaga acaagacaat gacgggaacc
541 aagagcaaga gtttctacga taccaacatc gacaaggagt aaaggaagaa caagacaatg
601 acgggaacaa cattttcagc ggcttcaaca gggatttctt ggaagatgct ttcaacgtga
661 acaggcatat agtagacaga cttcaaggca ggaacgaaga gaggggagcc attgtcaaag
721 tgaaaggtgg tctcagcatc ataacccctc cggagaggca agcgcgccac ccgagaggca
781 gcagacaaga agaagatgaa gatgaagatg aagatgaaaa ggaagagaga caaccaagcc
841 atcataaaag cagaagaggt gaagatgaag acgacaagga aaagcgccac agccaaaaag
901 gtgaaagcag aaggcatgga gacaatgggc ttgaggaaac cgtttgcact gccaaacttc
961 ggctgaacat tggttcatct tcatcgccag acatctacaa ccctcaagct ggtagaatca
1021 aaactgttac cagcgttgac ctcccagttc tcaggtggct caaactaagt gctgagcatg
1081 gatctctccg caaaaatgct atgtttgtgc ctcactacaa cctcaacgca aacagcgtat
1141 tatacgcatt gaagggacgt gcaaggctac aagttgtgaa ctgcaatggc aacactgtgt
1201 ttgatggaga gctagaagcc ggtcgtgcat tgacagtgcc acaaaactat gttgtggctg
1261 caaaatcatt gagcgacagg ttcacatatg tagcattcaa gacaaatgac agagctggta
1321 ttgcaagact agcagggaca tcatcagtta taaatgatct gcctttggat gtagtcgcag
1381 ctacattcaa cttggagagg aatgaggcaa ggcagctcaa gttcaacaat ccttccagat
1441 ttctagttcc accacgcgag tctgagaaga tttctagttc caccacgcga gtatgagaag
1501 agagcttccg cttagatttg tcatcaaatc gatgaaaata ttaataatga ataagaaagg
1561 taaggcttaa atggctttgt tgttg
//GenBank-Updates@genbank.bio.net (05/22/91)
LOCUS VFALEA2 1666 bp ss-mRNA PLN 22-MAY-1991
DEFINITION Vicia faba mRNA for Legumin A1 pre-pro-polypeptide
ACCESSION X55014
KEYWORDS legumin A; storage protein.
SOURCE Vicia faba RNA.
ORGANISM Vicia faba
Eukaryota; Plantae; Embryobionta; Magnoliophyta; Magnoliopsida;
Rosidae; Rosales; Fabaceaea.
REFERENCE 1 (sites)
AUTHORS Bassuener,R., Wobus,U. and Rapoport,T.A.
TITLE Signal recognition particle triggers the translocation of storage
globulin polypeptides from field beans (Vicia faba L.) across
mammalian endoplasmic reticulum membrane.
JOURNAL FEBS Lett. 166, 314-320 (1984)
STANDARD full staff_review
REFERENCE 2 (sites)
AUTHORS Horstmann,C.
TITLE New aspects of the subunit structure of Vicia faba legumin.
JOURNAL Kulturpflanze 32, 109-116 (1984)
STANDARD full staff_review
REFERENCE 3 (bases 1 to 1666)
AUTHORS Bassuener,R.
JOURNAL Unpublished (1990)
STANDARD full automatic
REFERENCE 4 (bases 1 to 1666)
AUTHORS Schlesier,B., Bassuener,R., Nong,V.H. and Muentz,K.
TITLE The cDNA derived primary structure of two distinct legumin A
subunit precursors from field bean (Vicia faba L.)
JOURNAL Nucleic Acids Res. 18, 7146-7146 (1990)
STANDARD full staff_review
COMMENT *source: strain=var. minor cv.Fribo, clone= Vfle 129
author's comment:"The supposed site of polyadenylation is four bp
downstream of the reported 3' terminal nucleotide." "For legumin A
encoding cDNA's of Vicia faba see: Wobus et al.: FEBS Lett. 201:
74-80(1986) and X55013; for legumin B encoding genes of Vicia faba
see: X03677; X14237; X14238; X14239; X14240; X14241." "The
classification is according to Nielsen et al.: The Plant Cell 1:
313-328(1989), as compared to other legumin-like seed storage
globulin subunits."
From EMBL 26 entry VFLEA2; dated 05-APR-1991.
FEATURES Location/Qualifiers
mRNA <1..>1666
/partial
/gene="LeA2"
/citation=[4]
/citation=[1]
sig_peptide 24..86
/product="signal peptide for cotranslational
translocation"
CDS 24..1526
/product="legumin A2 primary translation product"
/gene="LeA2"
/codon_start=24
/citation=[4]
/citation=[1]
/citation=[2]
mat_peptide 87..968
/note="The legumin mature subunit consists of one acidic
alpha and one basic beta chain, linked by a disulfide
bridge, and generated from the described pro-polypeptide
precursor."
/product="legumin A2 acidic alpha chain"
/gene="LeA2"
/citation=[4]
/citation=[2]
CDS 87..1526
/product="legumin A2 pro-polypeptide"
/gene="LeA2"
/codon_start=87
/citation=[4]
/citation=[1]
misc_feature 357..395
/note="hydrophilic segment"
misc_feature 576..644
/note="hydrophilic segment"
misc_feature 801..965
/note="hydrophilic segment"
repeat_region 840..857
/rpt_type=tandem
/rpt_unit=840..845
mat_peptide 969..1523
/note="The mature legumin subunit consists of one acidic
alpha and one basic beta chain, linked by a disulfide
bridge, and generated from the described pro-polypeptide
precursor."
/product="legumin A2 basic beta chain"
/gene="LeA2"
/citation=[4]
/citation=[2]
polyA_signal 1643..1648
BASE COUNT 537 a 359 c 385 g 385 t
ORIGIN
1 acattctcta aatatctgtt atcatggcta aacttcttgc actctctctt tcactttgct
61 ttctactttt cagtagctgc tttgctctca gagaacagtc tcaacaaaat gagtgtcagc
121 tagaacgcct cgatgccctt gaaccagata accgtataga gtcagaaggt ggacttattg
181 agacttggaa tccaaacaac aggcaattcc gatgtgcaag cgtggccctc tctcgtgcta
241 cccttcagcg caatgccctt cgcagacctt actattccaa tgctccccaa gaaatttaca
301 tccaacaagg taatggatat tttggcatgg tattccctag ttgtcctgag acctttgagg
361 agccccaaca atctgaacaa ggagagggag gcaggtacag agacagccac caaaaggtta
421 accgatttag agagggtgat atcattgcag ttcctactgg tattgtattt tggatgtaca
481 atgaccaaga tactccagtt attgccattt ctcttactga tattggcagc tccaataacc
541 agcttgatca gatgcctagg agattctatc ttgctgggaa ccaagagcaa gagtttctac
601 gataccaaca tcaacaagga ggaaaggaag aacaagacaa tgacgggaac aacattttca
661 gcggcttcaa gagggatttc ttggaagatg cgttgaacgt gaacaggcat atagtagaca
721 gacttcaagg caggaacgaa gacgaagaga agggagccat tgtcaaagtg aaaggtggtc
781 tcagcatcat aacccctccg gagaggcaag cgcgccaccc gagaggcagc agacaagaag
841 aagatgaaga tgaagatgaa aaggaagaga gacaaccaag ccatcataaa agcagaagag
901 gtgaagatga agacgacaag gaaaagcgcc acagccaaaa aggtgaaagc agaaggcatg
961 gagacaatgg gcttgaggaa accgtttgca ctgccaaact tcggctgaac attggttcat
1021 cttcatcgcc agacatctac aaccctcaag ctggtagaat caaaactgtt accagccttg
1081 acctcccagt tctcaggtgg ctcaaactaa gtgctgagca tggatctctc cgtaaaaatg
1141 ctatgtttgt gcctcactac aacctcaacg caaacagcat attatacgca ttgaagggac
1201 gtgcaaggct acaagttgtg aactgcaatg gcaacactgt gtttgatgaa gagctagaag
1261 ccggtcgtgc attgacagtg ccacaaaact atgctgtggc tgcaaaatca ttgagcgaca
1321 ggttcacata tgtagcattc aagaccaatg atagagctgg tattgcaaga cttgcaggga
1381 catcatcagt tataaatgat atgccggtgg atgtggttgc agctacattc aacttggaga
1441 ggaatgaggc aaggcagctc aagtccaaca atcccttcaa atttctagtt ccaccgcgtg
1501 agtctcagaa gagagcttcc gcttagattt gtcatcaaat cgatgaaaat aataatgaaa
1561 ataatgaata agaaaactaa ggcttagatg cctatgttgt tggtgtaaaa taacacgagt
1621 catgtacctt ttgcggcaac agaataaaca aagaagcgaa gttcca
//