GenBank-Updates@genbank.bio.net (05/22/91)
LOCUS TRBPFRAB 5137 bp ds-DNA INV 22-MAY-1991
DEFINITION Trypanosoma brucei PFR-A and PFR-B gene for paraflagellar rod
protein
ACCESSION X14819
KEYWORDS PFR-A gene; PFR-B gene; paraflagellar rod protein.
SOURCE Trypanosoma brucei DNA.
ORGANISM Trypanosoma brucei
Eukaryota; Animalia; Protozoa; Sarcomastigophora; Mastigophora;
Kinetoplastida; Trypanosomatina; Trypanosomatidae.
REFERENCE 1 (bases 1 to 5137)
AUTHORS Seebeck,T.
JOURNAL Unpublished (1989)
STANDARD full automatic
REFERENCE 2 (bases 1 to 5135)
AUTHORS Schlaeppi,K., Deflorin,J. and Seebeck,T.
TITLE The major component of the paraflagellar rod of Trypanosoma brucei
is a single protein, encoded by two tandemly arranged genes
JOURNAL J. Cell Biol. 109, 1695-1709 (1989)
STANDARD full automatic
COMMENT The sequence overlaps with that reported by Parsons et. al. in Cell
38:309-316(1984).
From EMBL 26 entry TBPFRAB; dated 07-APR-1991.
FEATURES Location/Qualifiers
CDS 552..2354
/note="(AA 1-600)"
/product="PFR-A protein"
/gene="PFR-A"
/codon_start=552
CDS 2706..4508
/note="(AA 1-600)"
/product="PFR-B protein"
/gene="PFR-B"
/codon_start=2706
BASE COUNT 1390 a 1120 c 1408 g 1219 t
ORIGIN
1 gacaaaactt gaagaccacc acattgaggc gactgcgtgt tggagtctac ttgaaccatt
61 aactgaacac agcgagttag cgataacatg gctgcaggaa tcactgtttc atactcctca
121 aggactgctc gcatggatca tcgcctgcac taacaacaat tgcaaccaaa cggcttttgt
181 gatggctgca tgggtgacac ttttatgccg cactgaaaag gtaggtgggt cattcaataa
241 acatgtccaa caacatctac ggtccaaatg cacaaaaaaa caagtggaac aacttctgtt
301 gcttgatcac tgcaattaag atccaaagaa agcgcggact tgcatagcgc atctacaagg
361 gaaaacgaaa cgcataaaaa aaattaatta taaaaaaaat taattataaa aaaaagaaat
421 aacttccaca accaaaatat aaaaaaaaac atgaatacca ccatataaat caatcacttt
481 tttttcacca acatatagta tcaaataata agagaattaa ctttgtagat aaagaaagca
541 ataaagcatc aatgagcgga aaggaagttg aaggtgttgt gagtcctgcg gaccagcagc
601 agccagccgt cccggaggta acagatatca cgctggaggc cgcccgcaag cagaaaattc
661 acaacctgaa gttgaagacc gcctgccttt cgaatgagga atatgtccag gacctgcacg
721 tatccgagtg gagtgagacg cagaagcaga agctgcaggc tgcacacgag aaagcgcatg
781 aattgcttgc ctcagtggag ggtgggacga agtggagcct gacagaggcg tatgacatca
841 agaagctgat gcgcgtctgt ggtcttgaga tgtctgtgcg tgaactgtac aagccggagg
901 acaagccaca gttcatggag attgttgcac tcaagaagac aatgaacgaa ctgaagcaac
961 atcacaacaa gactcgcacg gtgtctttca ccggcatgat cgacaatgcc atcgccaaac
1021 tggagaaaat cgaagacgaa ctgcgccggt cccagctcga cgcttctgag atggcgcaag
1081 ttcctgtggc tgcactgaag aatattgagg acacgatgaa cgtggctgtt gtgcagacgg
1141 ctcttcttgg gaacgaggag cagatcaaag cccaacttgc agccgttgag aaggcgaacg
1201 aaatccgtaa tgttgccatt gccgatggtg agatggcgat tgctgaggaa cagtattaca
1261 ttaaggcgca gctgttggag caccttgtgg agcttgtggc cgacaagttt cgcatcattg
1321 ggcaaactga ggatgagaat aagagcttca gtaagatcca cgaggtacag aagaagtcat
1381 ttcaggaatc tgcctcaatc aaggacgcga agcgccgcct taagcaacac tgcgaggacg
1441 acctacgtaa ccttcacgat gccatccaga aagctgactt ggaggacgcc gaagccatga
1501 aacggttcgc cacgcagaag gagaagtcgg agcggttcat ccacgagaac ctcgacaaac
1561 aggacgaggc atggcgtcgc attcaggaac tggagcgcgt gttgcagcgc cttgggacgg
1621 agcgttttga agaggtgaag cgccgtattg aggagaacga ccgcgaggag aagcgtaagg
1681 tggagtacca acagttcctc gatgtatgtg gccagcataa aaagctgctg gaactgtctg
1741 tgtacaactg cgaccttgcg cttcgctgca tgggtatgct ggaggagatc gtagccgagg
1801 gctgcagtgc cgtcaagtca cgccatgaca agacgaacga tgagttgtct gaccttcggc
1861 tgcaggtgca ccaggagtac ctggaggcat tccgtcgcct gtacaaaact cttggccagc
1921 ttgtgtacaa gaaagaaaag cgcctggagg agattgatcg caacatccgc accacacaca
1981 ttcaactgga gtttgccatt gagacctttg accccaacgc gaaactacac tccgacaaga
2041 agaaagacct atacaaactt cgtgcgcagg tggaggaaga gttggagatg ctgaaggaca
2101 agatggcgca ggcgttggag atgtttggac ctactgagga tgcgctgaac caggctggta
2161 tcgattttgt tcaccctgct gaggaggttg agtccggcaa catggatcgc cgcagcaaga
2221 tggtggagta ccgtgcacac ctggcgaagc aggaggaggt gaagattgcc gcggagcgcg
2281 aggagctgaa acgatctaag atgctccaga gccagcagta ccgcggccgc acgatgccgc
2341 agatcactca gtagcgctgc gcttaaatgt ctttcattat aatcaatgta taacctttat
2401 gtagtatttc aatctatgcc gctgtgtacg tgcactgcgg tgcctatcct tcggcattag
2461 agagtcactg tttgtgtaga tcgtagctgc atgtctgaca tctggttcat tagtgccgtt
2521 ttgagtctgc actgttgtgc aacttgatat gcatcactgc acaccaaccg tgtgtttcct
2581 cctttgggtg caccctttaa tccttgtctt ctcctttttt gtctctttcc ccctcgaaaa
2641 ggtgtcaaac tactgccgca taaactacgg ttatcccaaa tttaagagaa agcaataaag
2701 catcaatgag cggaaaggaa gttgaaggtg ttgtgagtcc tgcggaccag cagcagccag
2761 ccgtcccgga ggtaacagat atcacgctgg aggccgcccg caagcagaaa attcacaacc
2821 tgaagttgaa gaccgcctgc ctttcgaatg aggaatatgt ccaggacctg cacgtatccg
2881 agtggagtga gacgcagaag cagaagctgc aggctgcaca cgagaaagcg catgaattgc
2941 ttgcctcagt ggagggtggg acgaagtgga gcctgacaga ggcgtatgac atcaagaagc
3001 tgatgcgcgt ctgtggtctt gagatgtctg tgcgtgaact gtacaagccg gaggacaagc
3061 cacagttcat ggagattgtt gcactcaaga agacaatgaa cgaactgaag caacatcaca
3121 acaagactcg cacggtgtct ttcaccggca tgatcgacaa tgccatcgcc aaactggaga
3181 aaatcgaaga cgaactgcgc cggtcccagc tcgacgcttc tgagatggcg caagttcctg
3241 tggctgcact gaagaatatt gaggacacga tgaacgtggc tgttgtgcag acggctcttc
3301 ttgggaacga ggagcagatc aaagcccaac ttgcagccgt tgagaaggcg aacgaaatcc
3361 gtaatgttgc cattgccgat ggtgagatgg cgattgctga ggaacagtat tacattaagg
3421 cgcagctgtt ggagcacctt gtggagcttg tggccgacaa gtttcgcatc attgggcaaa
3481 ctgaggatga gaataagagc ttcagtaaga tccacgaggt acagaagaag tcatttcagg
3541 aatctgcctc aatcaaggac gcgaagcgcc gccttaagca acactgcgag gacgacctac
3601 gtaaccttca cgatgccatc cagaaagctg acttggagga cgccgaagcc atgaaacggt
3661 tcgccacgca gaaggagaag tcggagcggt tcatccacga gaacctcgac aaacaggacg
3721 aggcatggcg tcgcattcag gaactggagc gcgtgttgca gcgccttggg acggagcgtt
3781 ttgaagaggt gaagcgccgt attgaggaga acgaccgcga ggagaagcgt aaggtggagt
3841 accaacagtt cctcgatgta tgtggccagc ataaaaagct gctggaactg tctgtgtaca
3901 actgcgacct tgcgcttcgc tgcatgggta tgctggagga gatcgtagcc gagggctgca
3961 gtgccgtcaa gtcacgccat gacaagacga acgatgagtt gtctgacctt cggctgcagg
4021 tgcaccagga gtacctggag gcattccgtc gcctgtacaa aactcttggc cagcttgtgt
4081 acaagaaaga aaagcgcctg gaggagattg atcgcaacat ccgcaccaca cacattcaac
4141 tggagtttgc cattgagacc tttgacccca acgcgaaact acactccgac aagaagaaag
4201 acctatacaa acttcgtgcg caggtggagg aagagttgga gatgctgaag gacaagatgg
4261 cgcaggcgtt ggagatgttt ggacctactg aggatgcgct gaaccaggct ggtatcgatt
4321 ttgttcaccc tgctgaggag gttgagtccg gcaacatgga tcgccgcagc aagatggtgg
4381 agtaccgtgc acacctggcg aagcaggagg aggtgaagat tgccgcggag cgcgaggagc
4441 tgaaacgatc taagatgctc cagagccagc agtaccgcgg ccgcacgatg ccgcagatca
4501 ctcagtagga ttgtgtactg taattgtatt ttttggtttt tttttgaaag tggtagtagt
4561 attttagagt tgctacttac ttatgagttt gatatgtttg tttttatgta taattttttt
4621 tttgagaaac tgatgtttat atgtatgtgt atcttcttat ttgcggttag attagacgat
4681 ttttagtgta tttttagttg gctgggggtt atttggctgc tatttgttgt aggatgcgcc
4741 gtgctgacgc ttcttgctct tttatgtcgc cgagttatgc tgattgttgc tctctctata
4801 tttcttttgt gcggtggtgt tgttgaaaat ttttgctgct aatattgttg tcatgtattt
4861 tgttgttgtt acttgtctgt gagtggtttc tttctttctt tttttcactg tgttttgtaa
4921 tagtatctta ctttagctta ggattctagg tttggaggga tgtgttattg cctgatcaaa
4981 tatttctgtt ggagttgtta ggttactgtt gcacgtgact tccgtatata tccgtgttgg
5041 ctttttcttt ttgagttaaa gttcttttgt tttggtataa tcgtgtgtgt gggatctgct
5101 gcatgtaatg accggatggt cgagccgacg tggtacc
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