GenBank-Updates@genbank.bio.net (03/22/91)
LOCUS BSTGLNQH 2227 bp ds-DNA BCT 22-MAR-1991
DEFINITION B.stearothermophilus membrane transport protein (glnQ) and
glutamine-binding protein (glnH) genes, complete cds.
ACCESSION M61017
KEYWORDS glnH gene; glnQ gene; glutamine-binding protein;
membrane transport protein.
SOURCE B.stearothermophilus (strain NUB36) DNA.
ORGANISM Bacillus stearothermophilus
Prokaryota; Bacteria; Firmicutes; Endospore-forming rods and cocci;
Bacillaceae.
REFERENCE 1 (bases 1 to 2227)
AUTHORS Wu,L. and Welker,N.E.
TITLE Cloning and characterization of a glutamine transport system of
Bacillus stearothermophilus NUB36 that is regulated by temperature
JOURNAL Unpublished (1991)
STANDARD full staff_entry
FEATURES Location/Qualifiers
protein_bind 55..69
/note="nitrogen-regulator I binding site"
-35_signal 263..268
/gene="gln operon"
promoter 274..290
/note="nitrogen-regulated"
-10_signal 288..293
/gene="gln operon"
mRNA 301..2012
/gene="gln operon"
RBS 323..330
/gene="glnQ"
CDS 339..1067
/product="membrane transport protein"
/gene="glnQ"
/codon_start=339
CDS 1111..1899
/product="glutamine-binding protein"
/gene="glnH"
/codon_start=1111
sig_peptide 1111..1185
/gene="glnH"
/codon_start=1111
mat_peptide 1186..1896
/product="glutamine-binding protein"
/gene="glnH"
/codon_start=1186
RBS 1196..1104
/gene="glnH"
terminator 1978..2012
/gene="gln operon"
BASE COUNT 733 a 462 c 501 g 531 t
ORIGIN
1 ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg
61 gcgtggcctc gcttactttc agccctgttc gtccaagctt ccggtatttc atcattttct
121 ccccttttcg aaaatatgtc caaatccact catacacacc atcattataa tatttttaat
181 gtttaggcgt tagtaaattt tatacaatat ttcgatacct attacatccc acatcgctct
241 tttcagaaga ttatcgtcgt tttcggtaga ttgctgtagt ttgtgtttac gatgaattct
301 gaaaacaaaa ataatttatg aaaaaggagg ggggtctatt gatttatttt caccaagtaa
361 acaaatatta cggcgatttc catgtgctaa aagacattaa tttaacgatt catcaagggg
421 aagttgtcgt cattatcggg ccatcggggt cgggaaaaag cacgttagtt cgctgcatca
481 atcgcttgga aacgatttca agcggcgaat taatcgtgga taatgtcaaa gtcaatgaca
541 aacacatcga cattaaccaa ctgcggcgca acatcggcat ggtgtttcag cattttaact
601 tgtatccgca catgacggta ttgcaaaaca ttacgctagc gccgatgaaa gtgctacgca
661 ttccagaaaa agaagcgaaa gaaacagcga tgtattattt ggaaaaagtc ggcattcctg
721 ataaagcaaa cgcctaccct tccgaactgt ccggtggcca gcagcagcgt gtagcgatcg
781 ccagaggctt ggcgatgaag ccgaaaatta tgctctttga cgagccaacg tcagcgcttg
841 acccggaaac gatcggcgaa gtgctcgacg tcatgaaaca attagcaaaa gaaggcatga
901 ccatggttgt tgtgacacac gaaatgggat ttgcccgtga agtagctgat cgtatcgtct
961 ttatggatca aggccgcatt ttagaagaag cgccgccgga agagtttttc tccaatccaa
1021 aagaagaacg agcaaaagta tttttgagcc gcattctcaa ccattaataa gcaaaaaaat
1081 atttcgaatc aaaacaaagg aggttttttc atgaaaagaa aaacagtttg gaaaatatgg
1141 atcacgcttg cacttatcgc tttattgagc attactgctt tggctggatg cagcagcgaa
1201 tcgtccacga ctaacaaaga ggacggggca aaatcgacag aaacgtcggc tgggacgaac
1261 acgttagaaa aaattaaaaa acgcggcaaa ctgattgtcg gcgtcaaata tgacttaaac
1321 ctgtttggac tcaaaaatcc ggaaaccgga aaagtagaag gatttgatat cgatatcgcc
1381 aaaggattgg cgaaaaaaat tctcggtgac gaaaacaaaa tcgaattaaa agaagtcaca
1441 tccaaaacac gcatcccaat gctgaataat ggcgaaatcg acgcgattat cggcacgatg
1501 accatcacgg aagagcggaa aaaagaagtg gatttctccg atgtctattt tatggccggc
1561 caatcgctgc tcgtcaaaaa agatagcaag attaacagcg taaaagattt gaaaaaaggc
1621 atgaccgtat taaccgccaa aggctcaact tccgcgcaaa acattcgcaa agtagcacca
1681 gaagtcaatg tattagaatt tgaaaactac gccgaagcat tcacggcctt aaaagctgga
1741 caaggtgatg ccctcacgac ggacaatgcg ctgctttggg gaatggcaaa acaagatcca
1801 aactaccgcg tccttgacga aacgtttagc aaagaaccat acggcatcgg ctccgcaaag
1861 gagacaaaga attattgcaa gtcattaacg aatacttaaa agaaattaag gaaaacggcg
1921 aatacgacaa aatttacgaa aaatggatcg gaaaaaaacc gcaacaataa tactgaaaaa
1981 cagatgagcg gtatggtttc gctcatctgt ttcatcttca gaccggaaac gaggtgaaag
2041 ccgccatgtt acgatattcg attttgtttg accattggga tatgtatatg caagggtttg
2101 ccaacacatt aaaagccagt gttcttgccc tcatcggaag cttagccatc ggcatcatta
2161 tcgccatttt tcggattgcg ccgatccgtc cgttaaactg gcttggagcg gcctatgtcg
2221 aattcgg
//GenBank-Updates@genbank.bio.net (05/23/91)
LOCUS BACGLNQH 2227 bp ds-DNA BCT 23-MAY-1991
DEFINITION B.stearothermophilus membrane transport protein (glnQ) and
glutamine-binding protein (glnH) genes, complete cds.
ACCESSION M61017
KEYWORDS glnH gene; glnQ gene; glutamine-binding protein;
membrane transport protein.
SOURCE B.stearothermophilus (strain NUB36) DNA.
ORGANISM Bacillus stearothermophilus
Prokaryota; Bacteria; Firmicutes; Endospore-forming rods and cocci;
Bacillaceae.
REFERENCE 1 (bases 1 to 2227)
AUTHORS Wu,L. and Welker,N.E.
TITLE Cloning and characterization of a glutamine transport system of
Bacillus stearothermophilus NUB36 that is regulated by temperature
JOURNAL Unpublished (1991)
STANDARD full staff_entry
FEATURES Location/Qualifiers
protein_bind 55..69
/note="nitrogen-regulator I binding site"
-35_signal 263..268
/gene="gln operon"
promoter 274..290
/note="nitrogen-regulated"
-10_signal 288..293
/gene="gln operon"
mRNA 301..2012
/gene="gln operon"
RBS 323..330
/gene="glnQ"
CDS 339..1067
/product="membrane transport protein"
/gene="glnQ"
/codon_start=339
CDS 1111..1899
/product="glutamine-binding protein"
/gene="glnH"
/codon_start=1111
sig_peptide 1111..1185
/gene="glnH"
/codon_start=1111
mat_peptide 1186..1896
/product="glutamine-binding protein"
/gene="glnH"
/codon_start=1186
RBS 1196..1204
/gene="glnH"
terminator 1978..2012
/gene="gln operon"
BASE COUNT 733 a 462 c 501 g 531 t
ORIGIN
1 ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg
61 gcgtggcctc gcttactttc agccctgttc gtccaagctt ccggtatttc atcattttct
121 ccccttttcg aaaatatgtc caaatccact catacacacc atcattataa tatttttaat
181 gtttaggcgt tagtaaattt tatacaatat ttcgatacct attacatccc acatcgctct
241 tttcagaaga ttatcgtcgt tttcggtaga ttgctgtagt ttgtgtttac gatgaattct
301 gaaaacaaaa ataatttatg aaaaaggagg ggggtctatt gatttatttt caccaagtaa
361 acaaatatta cggcgatttc catgtgctaa aagacattaa tttaacgatt catcaagggg
421 aagttgtcgt cattatcggg ccatcggggt cgggaaaaag cacgttagtt cgctgcatca
481 atcgcttgga aacgatttca agcggcgaat taatcgtgga taatgtcaaa gtcaatgaca
541 aacacatcga cattaaccaa ctgcggcgca acatcggcat ggtgtttcag cattttaact
601 tgtatccgca catgacggta ttgcaaaaca ttacgctagc gccgatgaaa gtgctacgca
661 ttccagaaaa agaagcgaaa gaaacagcga tgtattattt ggaaaaagtc ggcattcctg
721 ataaagcaaa cgcctaccct tccgaactgt ccggtggcca gcagcagcgt gtagcgatcg
781 ccagaggctt ggcgatgaag ccgaaaatta tgctctttga cgagccaacg tcagcgcttg
841 acccggaaac gatcggcgaa gtgctcgacg tcatgaaaca attagcaaaa gaaggcatga
901 ccatggttgt tgtgacacac gaaatgggat ttgcccgtga agtagctgat cgtatcgtct
961 ttatggatca aggccgcatt ttagaagaag cgccgccgga agagtttttc tccaatccaa
1021 aagaagaacg agcaaaagta tttttgagcc gcattctcaa ccattaataa gcaaaaaaat
1081 atttcgaatc aaaacaaagg aggttttttc atgaaaagaa aaacagtttg gaaaatatgg
1141 atcacgcttg cacttatcgc tttattgagc attactgctt tggctggatg cagcagcgaa
1201 tcgtccacga ctaacaaaga ggacggggca aaatcgacag aaacgtcggc tgggacgaac
1261 acgttagaaa aaattaaaaa acgcggcaaa ctgattgtcg gcgtcaaata tgacttaaac
1321 ctgtttggac tcaaaaatcc ggaaaccgga aaagtagaag gatttgatat cgatatcgcc
1381 aaaggattgg cgaaaaaaat tctcggtgac gaaaacaaaa tcgaattaaa agaagtcaca
1441 tccaaaacac gcatcccaat gctgaataat ggcgaaatcg acgcgattat cggcacgatg
1501 accatcacgg aagagcggaa aaaagaagtg gatttctccg atgtctattt tatggccggc
1561 caatcgctgc tcgtcaaaaa agatagcaag attaacagcg taaaagattt gaaaaaaggc
1621 atgaccgtat taaccgccaa aggctcaact tccgcgcaaa acattcgcaa agtagcacca
1681 gaagtcaatg tattagaatt tgaaaactac gccgaagcat tcacggcctt aaaagctgga
1741 caaggtgatg ccctcacgac ggacaatgcg ctgctttggg gaatggcaaa acaagatcca
1801 aactaccgcg tccttgacga aacgtttagc aaagaaccat acggcatcgg ctccgcaaag
1861 gagacaaaga attattgcaa gtcattaacg aatacttaaa agaaattaag gaaaacggcg
1921 aatacgacaa aatttacgaa aaatggatcg gaaaaaaacc gcaacaataa tactgaaaaa
1981 cagatgagcg gtatggtttc gctcatctgt ttcatcttca gaccggaaac gaggtgaaag
2041 ccgccatgtt acgatattcg attttgtttg accattggga tatgtatatg caagggtttg
2101 ccaacacatt aaaagccagt gttcttgccc tcatcggaag cttagccatc ggcatcatta
2161 tcgccatttt tcggattgcg ccgatccgtc cgttaaactg gcttggagcg gcctatgtcg
2221 aattcgg
//