GenBank-Updates@genbank.bio.net (04/23/91)
LOCUS SYNHMG1 924 bp ds-DNA SYN 23-APR-1991
DEFINITION Rat high mobility group 1 protein synthetic gene, complete cds.
ACCESSION M63852
KEYWORDS high mobility group 1 protein.
SOURCE Rat and plasmid from E.coli DNA.
ORGANISM Artificial gene
Artificial sequences; Genes.
REFERENCE 1 (bases 1 to 924)
AUTHORS Bianchi,M.E.
TITLE Production of functional rat HMG1 protein in Escherichia coli
JOURNAL Unpublished (1991)
STANDARD full staff_entry
FEATURES Location/Qualifiers
misc_feature 1..88
/note="sequence from plasmid pT7-7"
promoter 9..31
/note="T7"
/evidence=EXPERIMENTAL
RBS 75..80
/evidence=EXPERIMENTAL
misc_feature 89..909
/note="sequence from plasmid pRNHMG1"
CDS 89..736
/product="high mobility group 1 protein"
/evidence=EXPERIMENTAL
/codon_start=89
misc_feature 890..881
/note="sequence from accession number Y00463"
misc_feature 910..924
/note="sequence from plasmid pT7-7"
BASE COUNT 312 a 167 c 238 g 207 t
ORIGIN
1 ttcgaaatta atacgactca ctatagggag accacaacgg tttccctctt agaataattt
61 tgtttaactt taagaaggag atatacatat gggcaaagga gatcctaaga agccgagagg
121 caaaatgtcc tcatatgcat tctttgtgca aacctgccgg gaggagcaca agaagaagca
181 cccggatgct tctgtcaact tctcagagtt ctccaagaag tgctcagaga ggtggaagac
241 catgtctgct aaagaaaagg ggaaatttga agatatggca aaggctgaca aggctcgtta
301 tgaaagagaa atgaaaacct acatcccccc caaaggggag accaaaaaga agttcaagga
361 ccccaatgcc cccaagaggc ctccttcggc cttcttcttg ttctgttctg agtaccgccc
421 aaaaatcaaa ggcgagcatc ctggcttatc cattggtgat gttgcgaaga aattaggaga
481 gatgtggaac aacactgctg cggatgacaa gcagccctat gaaaagaagg ccgccaagct
541 gaaggagaag tatgagaagg atattgctgc ctacagagct aaaggaaaac ctgatgcagc
601 gaaaaagggg gtggtcaagg ctgagaagag caagaaaaag aaggaagagg aagacgacga
661 ggaggatgaa gaggatgagg aagaggagga agaagaggaa gatgaagatg aagaagaaga
721 tgatgatgat gaataagttg gttctagcgc agtttttttt tcttgtctat aaagcattta
781 acccccctgt acacaactca ctccttttaa agaaaaaaat tgaaatgtaa ggctgtgtaa
841 gatttgtttt taaactgtac agtgtctttt tttgtatagt tggggatcct ctagagtcga
901 ccggcatgca agcttatcat cgat
//GenBank-Updates@genbank.bio.net (05/23/91)
LOCUS SYNHMG1 924 bp ds-DNA SYN 23-MAY-1991
DEFINITION Rat high mobility group 1 protein synthetic gene, complete cds.
ACCESSION M63852
KEYWORDS high mobility group 1 protein.
SOURCE Rat and plasmid from E.coli DNA.
ORGANISM Artificial gene
Artificial sequences; Genes.
REFERENCE 1 (bases 1 to 924)
AUTHORS Bianchi,M.E.
TITLE Production of functional rat HMG1 protein in Escherichia coli
JOURNAL Unpublished (1991)
STANDARD full staff_entry
FEATURES Location/Qualifiers
misc_feature 1..88
/note="sequence from plasmid pT7-7"
promoter 9..31
/note="T7"
/evidence=EXPERIMENTAL
RBS 75..80
/evidence=EXPERIMENTAL
misc_feature 89..909
/note="sequence from plasmid pRNHMG1"
CDS 89..736
/product="high mobility group 1 protein"
/evidence=EXPERIMENTAL
/codon_start=89
misc_feature 910..924
/note="sequence from plasmid pT7-7"
BASE COUNT 312 a 167 c 238 g 207 t
ORIGIN
1 ttcgaaatta atacgactca ctatagggag accacaacgg tttccctctt agaataattt
61 tgtttaactt taagaaggag atatacatat gggcaaagga gatcctaaga agccgagagg
121 caaaatgtcc tcatatgcat tctttgtgca aacctgccgg gaggagcaca agaagaagca
181 cccggatgct tctgtcaact tctcagagtt ctccaagaag tgctcagaga ggtggaagac
241 catgtctgct aaagaaaagg ggaaatttga agatatggca aaggctgaca aggctcgtta
301 tgaaagagaa atgaaaacct acatcccccc caaaggggag accaaaaaga agttcaagga
361 ccccaatgcc cccaagaggc ctccttcggc cttcttcttg ttctgttctg agtaccgccc
421 aaaaatcaaa ggcgagcatc ctggcttatc cattggtgat gttgcgaaga aattaggaga
481 gatgtggaac aacactgctg cggatgacaa gcagccctat gaaaagaagg ccgccaagct
541 gaaggagaag tatgagaagg atattgctgc ctacagagct aaaggaaaac ctgatgcagc
601 gaaaaagggg gtggtcaagg ctgagaagag caagaaaaag aaggaagagg aagacgacga
661 ggaggatgaa gaggatgagg aagaggagga agaagaggaa gatgaagatg aagaagaaga
721 tgatgatgat gaataagttg gttctagcgc agtttttttt tcttgtctat aaagcattta
781 acccccctgt acacaactca ctccttttaa agaaaaaaat tgaaatgtaa ggctgtgtaa
841 gatttgtttt taaactgtac agtgtctttt tttgtatagt tggggatcct ctagagtcga
901 ccggcatgca agcttatcat cgat
//