GenBank-Updates@genbank.bio.net (04/30/91)
LOCUS BC1REP 1615 bp ds-DNA BCT 30-APR-1991
DEFINITION Plasmid pBC1 (from Bacillus coagulans) replicative protein (rep)
gene, complete cds.
ACCESSION M64604
KEYWORDS replicative protein.
SOURCE Plasmid pBC1 (from Bacillus coagulans, strain ZUI96I) DNA.
ORGANISM Plasmid pBC1
Prokaryota; Bacteria.
REFERENCE 1 (bases 1 to 1615)
AUTHORS De Rossi,E., Milano,A., Brigidi,P., Bini,F. and Riccardi,G.
TITLE Structural organization of pBC1, a cryptic plasmid from Bacillus
coagulans
JOURNAL Unpublished (1991)
STANDARD full staff_entry
FEATURES Location/Qualifiers
-35_signal 541..546
/gene="rep"
-10_signal 572..577
/gene="rep"
RBS 595..600
/gene="rep"
CDS 610..1389
/product="replicative protein"
/gene="rep"
/codon_start=610
rep_origin 59161..175
/note="plus strand"
/gene="rep"
BASE COUNT 518 a 289 c 394 g 414 t
ORIGIN
1 ccccgatggt ttttcccccg acggctcgcc tttgggaagt ttgaagggcg caggttccac
61 acgaaaaacc acaaggcttt ttttgagtaa taaaaaagcg gacggcgagg gcgtgaaaaa
121 aacccgatga acccttgaaa ataaagggcg ggacgggttt tttcttatct tgataatata
181 tagaaacaat gagattttaa aaaaagccct caaacccttg ataatactgg gtttgtggcg
241 tttttttggg gtggataaat aaaaaccctc tgtgttatgg ttatgttgac tagacaaaac
301 catacagagg gctttacgcc tttctgtatc cagaaaggag ttattaaatt ttatgcacgt
361 taatcatagc attaaaacgt caaatctatc aaatatcgaa tttttgcaag ataaaacgaa
421 aacaggaaaa gagagagatt ggaaaggcaa gaaacaacgg tctttgctga cagcggaaca
481 cttcgaggta gcagggctga ctagcaaagc ggaacagtgc gagagtgtgc tgacacgttg
541 gtgtttaagc gaactgccga agggttaaaa ctatatcaag catggttctg taaagtgagg
601 ttatgcccga tgtgcaattg gcgaagatcg ctgaaaatag cttaccagaa taaaagggtt
661 gtagaggcgg ttaatcaacg tgagaacgtt cagtggctat tcctaaccct taccgtccgc
721 aacacgagcc ctgagagcct tccagagacg atttcagcca tgtttgaggg gtttaatagg
781 ctgacgaagt acaaagcctt taaaacgtct gtaaagggct attttagggc tttagaggtc
841 acaaagaata gagaccctca tagtgaatgg tttggcacgt atcaccctca ttttcacgtt
901 ctgctgtgtg ttccatccag ctatttcaag aaaaaagaat tatacataac cgaacaagaa
961 tggactgacc tttggaaaaa ggctatgaag ttggattaca cgccgattgt ccacgtgcaa
1021 agggtaaaac ccaaagaaca gcttgaggac atggaaacct atgaagaaca gcttaaaaac
1081 gccattaggg aacaaaatgc gattttagaa gtctctaaat atccggtcaa agatacggac
1141 gtcattaaag ggaataaggt cacggccgaa aatgtggaaa ccgttttggc gttagacaac
1201 gccttggcaa ataagcggtt aatcgggtat ggcggtcttt tgaaacaggt tcacaaggaa
1261 ttaaaccttg gagatccgga agatggagat ttagttcatg tttcggaaga ggatgaaatc
1321 gctaatggtg catttgaggt catggcgaaa tggcatatcg gttttagaga ttattggatt
1381 caaaaatagc aggagagaaa actcctgctt ttttattttt tccgaagtta ttggcgaaag
1441 caaacttttt atcgagcgaa gcgaacccta ttgaatacct gcatggcaag gtatgtaaat
1501 gggcactctg tgatttttgg atacaaaata gactctagcg agccgatttt atgacgcagc
1561 aaaaaacgta gtctttttgc gttggagagc ccttcaagta aactgaccaa ggtgg
//GenBank-Updates@genbank.bio.net (05/23/91)
LOCUS BC1REP 1615 bp ds-DNA BCT 23-MAY-1991
DEFINITION Plasmid pBC1 (from Bacillus coagulans) replicative protein (rep)
gene, complete cds.
ACCESSION M64604
KEYWORDS replicative protein.
SOURCE Plasmid pBC1 (from Bacillus coagulans, strain ZUI96I) DNA.
ORGANISM Plasmid pBC1
Prokaryota; Bacteria.
REFERENCE 1 (bases 1 to 1615)
AUTHORS De Rossi,E., Milano,A., Brigidi,P., Bini,F. and Riccardi,G.
TITLE Structural organization of pBC1, a cryptic plasmid from Bacillus
coagulans
JOURNAL Unpublished (1991)
STANDARD full staff_entry
FEATURES Location/Qualifiers
rep_origin 161..175
/note="plus strand"
/gene="rep"
-35_signal 541..546
/gene="rep"
-10_signal 572..577
/gene="rep"
RBS 595..600
/gene="rep"
CDS 610..1389
/product="replicative protein"
/gene="rep"
/codon_start=610
BASE COUNT 518 a 289 c 394 g 414 t
ORIGIN
1 ccccgatggt ttttcccccg acggctcgcc tttgggaagt ttgaagggcg caggttccac
61 acgaaaaacc acaaggcttt ttttgagtaa taaaaaagcg gacggcgagg gcgtgaaaaa
121 aacccgatga acccttgaaa ataaagggcg ggacgggttt tttcttatct tgataatata
181 tagaaacaat gagattttaa aaaaagccct caaacccttg ataatactgg gtttgtggcg
241 tttttttggg gtggataaat aaaaaccctc tgtgttatgg ttatgttgac tagacaaaac
301 catacagagg gctttacgcc tttctgtatc cagaaaggag ttattaaatt ttatgcacgt
361 taatcatagc attaaaacgt caaatctatc aaatatcgaa tttttgcaag ataaaacgaa
421 aacaggaaaa gagagagatt ggaaaggcaa gaaacaacgg tctttgctga cagcggaaca
481 cttcgaggta gcagggctga ctagcaaagc ggaacagtgc gagagtgtgc tgacacgttg
541 gtgtttaagc gaactgccga agggttaaaa ctatatcaag catggttctg taaagtgagg
601 ttatgcccga tgtgcaattg gcgaagatcg ctgaaaatag cttaccagaa taaaagggtt
661 gtagaggcgg ttaatcaacg tgagaacgtt cagtggctat tcctaaccct taccgtccgc
721 aacacgagcc ctgagagcct tccagagacg atttcagcca tgtttgaggg gtttaatagg
781 ctgacgaagt acaaagcctt taaaacgtct gtaaagggct attttagggc tttagaggtc
841 acaaagaata gagaccctca tagtgaatgg tttggcacgt atcaccctca ttttcacgtt
901 ctgctgtgtg ttccatccag ctatttcaag aaaaaagaat tatacataac cgaacaagaa
961 tggactgacc tttggaaaaa ggctatgaag ttggattaca cgccgattgt ccacgtgcaa
1021 agggtaaaac ccaaagaaca gcttgaggac atggaaacct atgaagaaca gcttaaaaac
1081 gccattaggg aacaaaatgc gattttagaa gtctctaaat atccggtcaa agatacggac
1141 gtcattaaag ggaataaggt cacggccgaa aatgtggaaa ccgttttggc gttagacaac
1201 gccttggcaa ataagcggtt aatcgggtat ggcggtcttt tgaaacaggt tcacaaggaa
1261 ttaaaccttg gagatccgga agatggagat ttagttcatg tttcggaaga ggatgaaatc
1321 gctaatggtg catttgaggt catggcgaaa tggcatatcg gttttagaga ttattggatt
1381 caaaaatagc aggagagaaa actcctgctt ttttattttt tccgaagtta ttggcgaaag
1441 caaacttttt atcgagcgaa gcgaacccta ttgaatacct gcatggcaag gtatgtaaat
1501 gggcactctg tgatttttgg atacaaaata gactctagcg agccgatttt atgacgcagc
1561 aaaaaacgta gtctttttgc gttggagagc ccttcaagta aactgaccaa ggtgg
//