GenBank-Updates@genbank.bio.net (05/23/91)
LOCUS MUSIGKWF 1020 bp ds-DNA ROD 23-MAY-1991
DEFINITION Mouse Ig rearranged kappa chain gene 25-39, V-region (V-J), from a
hybridoma.
ACCESSION M18772
KEYWORDS immunoglobulin; immunoglobulin light-chain; joining region;
kappa-immunoglobulin; processed gene; variable region.
SOURCE Mouse anti-streptococcal group A carbohydrate hybridoma DNA.
ORGANISM Mus musculus
Eukaryota; Animalia; Metazoa; Chordata; Vertebrata; Mammalia;
Theria; Eutheria; Rodentia; Myomorpha; Muridae; Murinae.
REFERENCE 1 (bases 976 to 1020)
AUTHORS Lutz,C.T.
JOURNAL Unpublished (1988)
STANDARD full staff_review
REFERENCE 2 (bases 1 to 976)
AUTHORS Lutz,C.T. and Davie,J.M.
TITLE Genetics and primary structure of V-kappa gene segments encoding
antibody to streptococcal group A carbohydrate: Comparison of
V-kappa gene structure with idiotope expression
JOURNAL J. Immunol. 140, 641-645 (1988)
STANDARD full staff_review
COMMENT Draft entry and computer-readable sequence [2] kindly provided by
C.T.Lutz, 06-APR-1988. The rearranged Vk differs for the germline
Vk at 3 site: 787; a to t, 791; g to a, and 803; g to c.
FEATURES Location/Qualifiers
CDS join(247..295,667..976)
/partial
/note="Ig kappa chain V-region (V-J) precursor"
sig_peptide join(247..295,666..677)
/note="signal peptide"
exon <247..295
/note="Ig kappa chain V-region (V-J) precursor, exon 1"
sig_peptide 247..295
/note="signal peptide exon 1"
intron 296..666
/note="Ig kappa chain intron A"
sig_peptide 666..677
/note="signal peptide, exon 2"
exon 667..>976
/note="Ig kappa chain V-region (V-J) precursor, exon 2"
mat_peptide 678..>976
/partial
/note="Ig kappa chain V-region (V-J)"
misc_recomb 976^977
/note="V-region end; J-region start"
misc_recomb 1014^1015
/note="J-region end; intervening DNA start"
iDNA 1015..1020
/note="intervening DNA"
BASE COUNT 285 a 196 c 231 g 308 t
ORIGIN Chromosome 6.
1 gaattcatct gccactaata ttggcagtga ggtctgagtt aattttctac aaacatgaaa
61 tgaagtttgt caagtgacct ttgagtgtgg gatttttaac tctctaggta ctttttaaaa
121 taactctatc aaccaggaga tttgcatatt gctccctagg gacaacctct tcttgcaagt
181 gcagggtaaa agctcactcc tctttctgtc ttgattactt ttgactctcc atatcaagtt
241 cgcagaatga ggttctctgc tcagcttctg gggctgcttg tgctctggat ccctggtaag
301 gggacaaaga tgaagaagga gaattttgag ggagggggat ttctgagaca tgatgataaa
361 tatgtatatt ttgtctatgt atgagatata cagttctgtt ctccagtaaa ggacttgtga
421 ggttcaaagt gtgaagagat taaaatctgt ttttctgtga caactctgac aggttccaaa
481 gccaaaagtc aaggtgaatg ctagaatgaa gactctctgt gcttcctcta catgcatatt
541 tcatgtggag cacttttaga gtatgagtga aagacatgaa cacaataagt agaaaaaaat
601 aaaggaggga aattcacttc attgtatcat ttttttgtac ataaccaatt aattctctta
661 ttgcaggatc cactgcagat attgtgatga cgcaggctgc cttctccaat ccagtcactc
721 ttggaacatc agcttccatc tcctgcaggt ctagtaagaa tctcctacat agtaatggca
781 tcactttttt atattggtat ctccagaggc caggccagtc tcctcagctc ctgatatatc
841 gggtgtccaa tctggcctca ggagtcccaa acaggttcag tggcagtgag tcaggaactg
901 atttcacact gagaatcagc agagtggagg ctgaggatgt gggtgtttat tactgtgctc
961 aactgctaga actcccgtac acgttcggag gggggaccaa gctggaaata aaacgtaagt
//