GenBank-Updates@genbank.bio.net (05/25/91)
LOCUS MUSTCBXZR 519 bp ds-DNA ROD 25-MAY-1991
DEFINITION Mouse T-cell receptor rearranged beta-chain V17(V17a-D2-J2.5) from
hybridoma QK-24.1.
ACCESSION M16203
KEYWORDS T-cell receptor; T-cell receptor beta-chain; antigen receptor;
cell surface glycoprotein; diversity region; joining region;
variable region.
SOURCE Mouse (BALB/c) SWR T-cell hybridomas QK-24.1 DNA, clone PEMBL8.
ORGANISM Mus musculus
Eukaryota; Animalia; Metazoa; Chordata; Vertebrata; Mammalia;
Theria; Eutheria; Rodentia; Myomorpha; Muridae; Murinae.
REFERENCE 1 (bases 1 to 519)
AUTHORS Kappler,J.W., Wade,T., White,J., Kushnir,E., Blackman,M., Bill,J.,
Roehm,N. and Marrack,P.
TITLE A T cell receptor V-beta segment that imparts reactivity to a class
II major histocompatibility complex product
JOURNAL Cell 49, 263-271 (1987)
STANDARD full staff_review
COMMENT The study suggests that the V beta portion of the receptor may form
the most important contact points with MHC ligands.
FEATURES Location/Qualifiers
exon <6..53
/note="T-cell receptor beta-chain precursor
(V17a-D2-J2.5), exon 1"
CDS join(6..53,175..519)
/partial
/note="T-cell receptor beta-chain precursor
(V17a-D2-J2.5)"
/codon_start=6
sig_peptide join(6..53,175..189)
/note="T-cell receptor beta-chain signal peptide"
/codon_start=6
sig_peptide 6..53
/note="T-cell receptor beta-chain signal peptide"
intron 54..174
/note="intron A (no splice consensus at 54)"
sig_peptide 175..189
/note="T-cell receptor beta-chain signal peptide"
exon 175..>519
/note="T-cell receptor beta-chain precursor
(V17a-D2-J2.5), exon 2"
mat_peptide 190..>519
/partial
/note="T-cell receptor beta-chain"
/codon_start=190
misc_recomb 471^472
/note="V17.1-region end/ D2-region start"
misc_recomb 478^479
/note="D2-region end/ J2.5-region start"
BASE COUNT 139 a 129 c 114 g 137 t
ORIGIN 487 bp upstream of ScaI site.
1 tgatcatggg tgcaagactg ctctgctgtg tagcactttg tctgcttggg gcaggtaaga
61 agtgagtcaa aagggaaagg acttgtcctc acactccaca ctacagttcc caatgttttt
121 ccctttcaga gtccctcctg tcctgttctc attttctgtt tccccctctt ccaggctctt
181 tatgttgctg gagtaaccca gactccacga tacctggtca aagagaaagg acagaaagca
241 cacatgagtt gtagtcctga aaaagggcac actgcctttt actggtatca acagaaccag
301 aaacaagaac ttacattttt gattaacttt cgaaatgaag aaattatgga acaaacagac
361 ttggtcaaga agagattctc agctaagtgt tcctcgaact cacagtgcat cctggaaatc
421 ctatcctctg aagaagacga ctcagcactg tacctctgtg ccagcagtct agggggggac
481 acccagtact ttgggccagg cactcggctc ctcgtgtta
//