GenBank-Updates@genbank.bio.net (05/25/91)
LOCUS POABETA1 2293 bp ds-DNA PLN 25-MAY-1991
DEFINITION P.agilis beta-1 tubulin (beta-1-tub) gene, complete cds.
ACCESSION M33372
KEYWORDS beta-1 tubulin.
SOURCE P.agilis (strain Aragoa) DNA.
ORGANISM Polytomella agilis
Eukaryota; Plantae; Thallobionta; Chlorophycota; Chlorophyceae;
Volvocales; Dunaliellaceae.
REFERENCE 1 (bases 1 to 2293)
AUTHORS Conner,T.W., Thompson,M.D. and Silflow,C.D.
TITLE Structure of the three beta-tubulin-encoding genes of the
unicellular alga, Polytomella agilis
JOURNAL Gene 84, 345-358 (1989)
STANDARD simple staff_review
FEATURES Location/Qualifiers
CDS join(233..250,327..353,432..555,624..848,974..1911)
/note="beta-1 tubulin (beta-1-tub)"
/codon_start=233
misc_feature 80..161
/note="putative VECTOR sequence Bacteriphage M13mp18
(M11454)"
exon 233..250
/note="beta-1 tubulin (beta-1-tub), exon 1"
intron 251..326
/note="beta-1 tubulin intron A"
exon 327..353
/note="beta-1 tubulin, exon 2"
intron 354..431
/note="beta-1 tubulin intron B"
exon 432..555
/note="beta-1 tubulin, exon 3"
intron 556..623
/note="beta-1 tubulin intron C"
exon 624..848
/note="beta-1 tubulin, exon 4"
intron 849..973
/note="beta-1 tubulin intron D"
exon 974..1911
/note="beta-1 tubulin, exon 5"
BASE COUNT 524 a 578 c 464 g 727 t
ORIGIN
1 tttttttgta aggtttaaga ggtcatttgc ttcctaaatg gagtttaaaa aaattaaatg
61 tgtaggtgct ggttgattat atgcttccgg ctcgtatgtt gtgtggaatg tgagcggata
121 acaatttcac acaggaaaca gctatgacat gattacgaat tatatatatg tgccgtcctc
181 ccaaaaaatt taaattgaac tacgcctgtt ttgtcaattt aaccggtagc aaatgcgtga
241 aatcgttcat gtgagtaata tttatttgat atctgccact tactctcctt attctaatag
301 catcttctcc ttttcctcac ctacagattc aaggtggcca gtgtggtaac caggtgtgtt
361 tttcgccctc ttgcctgcta aatgttcgtt ttgactctat caacacctct aacatgcttc
421 tctccctgta gattggtgcc aagttctggg aagtcgtttc tgacgagcat ggcattgacc
481 ccaccggtac tgctcttggt gacagcgacc tccagcttga gcgcatcaac gtctacttca
541 acgaggccac tggaagtaag taatttgagc ccctcctcaa tatttttttc agctccaatt
601 aaaatctttt tttctcactc caggccgcta tgtcccccgt gccatcctca tggatcttga
661 gcccggtacc atggattccg tccgctctgg tccctatggc cagattttcc gccctgacaa
721 ctttgtcttc ggtcagactg gtgccggtaa caactgggct aagggccact atactgaggg
781 tgctgagctc attgactccg tccttgatgt tgtccgcaag gaggctgagt cctgcgattg
841 ccttcagggt aagaatttga tatcttttat ttctatgatt cttagattat tttttcaacc
901 taacatagtt aatatttcaa attgtatctc actgttcatt tttataaaat taatttttat
961 tatgtcttta aaggcttcca ggtctgccac tccctcggtg gtggtaccgg ctccggtatg
1021 ggaaccctcc ttatttccaa gatccgtgag gagtaccctg accgcatgat gctcaccttc
1081 tccgtcgtcc cctcccccaa ggtgtctgat accgtcgttg agccctacaa cgccaccctc
1141 tccgttcatc agctcgttga gaacgccgat gagtgcatgg tcctcgataa cgaggctctc
1201 tacgatatct gcttccgcac ccttaagctc accaccccca ccttcggtga ccttaaccat
1261 cttatctccg ccgtcatgtc cggcatcacc tgctgcctcc gcttccccgg tcagctcaac
1321 gctgatctcc gtaagcttgc cgtcaacctt atccccttcc cccgtctcca tttcttcatg
1381 gttggtttca cccccctcac ctcccgtggc agccagcagt accgtgccct caccgtcccc
1441 gagcttaccc agcagatgtg ggatgccaag aacatgatgt gcgccgctga tccccgccac
1501 ggtcgttacc tcaccgcctc cgcccttttc cgtggtcgta tgtccaccaa ggaggtcgat
1561 gagcagatgc ttaacgtcca gaacaagaac tcttcttact tcgttgagtg gatccccaac
1621 aatgtcaagt cctccgtttg cgatatccct cctaagggtc ttaagatgtc tgctaccttc
1681 attggtaaca gcactgctat ccaggagatg ttcaagcgtg tctctgagca gttcactgcc
1741 atgttcaggc gcaaggcttt cttgcattgg tatactggtg agggtatgga tgagatggag
1801 ttcactgagg ccgagtccaa catgaacgat ctcgtctccg agtaccagca gtaccaggat
1861 gcctctgctg aagaggaggg cgagttcggt gaggaagagg aggagaacta aactatcaca
1921 aactatttgt gaaactagcc gctgatttta aggtttatgt agaaggggga tacccacaat
1981 ttttgcttta atgattcagt ttatgtttaa ctgtatgtat gtttttttat aatgaatagc
2041 agtcactgtc cactctataa tgtaatttta ttttgttttc ttcactaata gcgtaatgcc
2101 gagagcttgt aatgataaag gacgttaatt acgaagagta atatagatat aaatattttt
2161 tacatgatgt ttataattat aataacaata aaatactttt tgactcttct aacgttaatg
2221 ttattattct ctatcctcgg tcacgtttac gtttttttta ccctttgttt tatttcctgc
2281 cgcatcttta taa
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