GenBank-Updates@genbank.bio.net (05/25/91)
LOCUS SERERYAA 11219 bp ds-DNA PLN 25-MAY-1991
DEFINITION S.erythraea first ORF of eryA gene, complete cds.
ACCESSION M63676
KEYWORDS 6-deoxyerythronolide B; acyltransferase;
beta-ketoacyl-ACP synthase I; beta-ketoreductase; eryA gene.
SOURCE Saccharopolyspora erythraea () DNA.
ORGANISM Saccharopolyspora erythraea
Prokaryota; Bacteria; Firmicutes; Nocardioform actinomycetes.
REFERENCE 1 (bases 1 to 11219)
AUTHORS Donadio,S., Staver,M.J., McAlpine,J.B., Swanson,S.J. and Katz,L.
TITLE Modular Organization of Genes Required for Complex Polyketide
Biosynthesis
JOURNAL Science 252, 675-679 (1991)
STANDARD full automatic
FEATURES Location/Qualifiers
CDS 744..11219
/note="ORF1 encoding modules 1 & 2 for
6-deoxyerythronolide B formation"
/gene="eryA"
/codon_start=744
misc_feature 744..6659
/function="'approximate span of module 1'"
misc_feature 744..1868
/function="'approximate span of acyltransferase domain 1
ofmodule 1'"
misc_feature 1998..2198
/function="'approximate span of acyl carrier domain 1 of
module1'"
misc_feature 2250..3626
/function="'approximate span of beta-ketoacyl ACP synthase
domain of module 1'"
misc_feature 3831..4811
/function="'approximate span of acyltransferase 2 domain
ofmodule 1'"
misc_feature 5574..6125
/function="'approximate span of beta-ketoreductase domain
ofmodule 1'"
misc_feature 6369..6626
/function="'approximate span of acyl carrier domain 2 of
module1'"
misc_feature 6678..11219
/function="'approximate span of module 2'"
misc_feature 6678..8066
/function="'approximate span of beta-ketoacyl ACP synthase
ofmodule 2'"
misc_feature 8262..9305
/function="'approximate span of acyltransferase domain of
module2'"
/function="'approximate span of beta-ketoacyl ACP synthase
ofmodule 2'"
misc_feature 9906..10454
/function="'approximate span of beta-ketoreductase domain
of module 2'"
misc_feature 10707..10964
/function="'approximate span of acyl carrier domain of
module2'"
BASE COUNT 1367 a 3949 c 4333 g 1570 t
ORIGIN
1 gtcgacctgc ggcgatcgtg cagcgcgccg acgaggtcgt gcatcaggcc gacgttgacc
61 cgctcggctt ccgggtcgga ggtggcgctg cgccaggtgg aaccgcccgc tgcgtgcgcc
121 accaggtgca cgatcacgtc ggcgtcctcg atcgcggcgg cggcccggcc cggttccagc
181 aggtcggcgc gcaggtcctc gacctccgcg gcgccgggcg gaaccgcggg cgctccgccg
241 cgggacaccg cgcgcagccg gaccgggtgg tcgcgcagct cgcgcagaac cgcgctcccg
301 acgaagccgg aagcgcccag aagggtgatc aattgacgcg gggaatcact gatcccattc
361 accggagcat ttgctcgctt tccaggtcgg tgctacgggc gaaattcaaa gaatctcccc
421 agcgcgatgt gcggcaaccc gtcactgggc caccacagta ggtagccgcc gttgatcttg
481 tcaacatgca gatgttcaca ggttcgttgg ctcgacgagg cgatgtcaac ctcttgatcc
541 ttcctatatt gttcgcccat tgcgtggtcg tcgagtaggg ggacgcgtgg cggacctgtc
601 aaagctctcc gacagtcgga ctgcacaacc tgggaggatc gttcgtccgt ggcccctgtc
661 ggggtgcaat gaatccgcct tgcgggcccg tgcgcgccaa ttgcgtgcac atctcgatcg
721 atttcccgat gccggtgtcg aaggtgtcgg ggccgcgctc gcgcacgacg agcaggcgga
781 cgccggtccg catcggcgcg gtcgtcgtcg cctcctcgac ctccgagctg ctcgacggcc
841 tggccgccgt cgccgacggc cggccgcacg cctcggtggt ccgcggcgtg gcccggccgt
901 ccgcgccggt ggtgttcgtc ttcccgggcc agggcgcgca atgggccggg atggcgggcg
961 aactcctcgg cgagtcaagg gttttcgccg ccgcgatgga cgcgtgcgcg cgggcgttcg
1021 agcccgtgac cgactggacg ctggcgcagg tcctggactc tcccgagcag tcgcgccgcg
1081 tcgaggtcgt ccagcccgcc ctgttcgcgg tgcagacgtc gctggccgcg ctctggcgct
1141 ccttcggcgt gacccccgac gccgtggtgg gccacagcat cggcgagctg gccgccgcgc
1201 acgtgtgcgg tgcggccggt gccgccgacg ccgcgcgcgc cgccgcgctg tggagccgcg
1261 agatgattcc gttggtgggc aacggcgaca tggcagccgt cgcgctctcc gccgacgaga
1321 tcgagccgcg catcgcccgg tgggacgacg acgtggtgct ggccggggtc aacggtccgc
1381 gctcggttct gctgaccggg tcgccggaac cggtcgcgcg ccgggtccag gagctctcgg
1441 ccgagggggt ccgcgcacag gtcatcaatg tgtcgatggc ggcgcactcg gcgcaggtcg
1501 acgacatcgc cgaggggatg cgctcggccc tggcgtggtt cgcgcccggt ggctcggagg
1561 tgcccttcta cgccagcctc accggaggtg cggtcgacac gcgggagctg gtggccgact
1621 actggcgccg cagcttccgg ctgccggtgc gcttcgacga ggcgatccgg tccgccctgg
1681 aggtcggtcc cggcacgttc gtcgaagcga gcccgcaccc ggtgctggcc gccgcgctcc
1741 agcagacgct cgacgccgag ggctcctcgg ccgcggtggt cccgacgctg caacgcgggc
1801 agggcggcat gcggcggttc ctgctggccg cggcccaggc gttcaccggc ggcgtggccg
1861 tcgactggac cgccgcctac gacgacgtgg ggccgaaccc ggctctctgc cggagttcgc
1921 gccggccgag gaggaagacg agccggccga gtccggcgtc gactggaacg cgccaccgca
1981 cgtgctgcga gcggctgctc gcggtcgtca acggcgagac cgccgcgttg gcgggccgcg
2041 aagccgacgc cgaggccacg ttccgcgagc tggggctgga ctcggtgctg gccgcgcagc
2101 tgcgcgccaa ggtgagcgcc gcgatcgggc gcgaggtcaa catcgccctg ctctacgacc
2161 acccgactcc gcgtgcgctc gcggaagcac tcgcggcggg aaccgaggtc gcacaacggg
2221 aaacccgcgc gcggaccaac gaagcggcgc ccggcgaacc ggtcgcggtc gtcgcgatgg
2281 cctgccggct gcccggcggt gtgagcaccc cggaggagtt ctgggagctg ctgtcggagg
2341 gccgcgacgc ggtcgcggga ctgccgaccg accgcggctg ggacctggac tcgctgttcc
2401 accccgaccc cacgcgctcg ggcaccgcgc accagcgcgg cggcggtttc ctgaccgagg
2461 cgaccgcgtt cgacccggcc ttcttcggca tgtccccgcg cgaggcgctg gccgtcgacc
2521 cgcagcagcg gctcatgctc gagctctcct gggaagtgct ggaacgggcg ggaatcccgc
2581 cgacctcgtt gcaggcctcg cccactggcg tgttcgtcgg cctgatcccg caggagtacg
2641 gcccgcggct ggccgagggc ggcgaaggcg tcgagggcta cctgatgacc ggtacgacca
2701 cgagcgtcgc ctccggccgc atcgcctaca cgctcggcct ggagggcccg gcgatcagcg
2761 tggacaccgc gtgctcgtcc tcgctggtcg cggtgcacct ggcgtgccag tcgctgcggc
2821 gcggcgagtc gtcgctggcg atggcaggcg gtgtcacggt gatgccgacg cccggcatgc
2881 tggtggactt cagccggatg aactcgctgg cgccggacgg ccggtgcaag gctttctccg
2941 ccggcgccaa cggtttcggc atggccgagg gcgccgggat gctcctgctg gagcggcttt
3001 cggacgcccg ccgcaacggc cacccggtgc tcgccgtgct cagggggacg gcggtcaact
3061 ccgacggcgc gagcaacggg ctgtcggcgc ccaacgggcg ggcgcaggtg cgggtcatcc
3121 agcaggcgct ggcagagtcc ggtctcgggc ccgccgacat cgacgccgtc gaggcgcacg
3181 gcaccggtac ccgactcggc gacccgatcg aggcgcgggc gctgttcgag gcgtacgggc
3241 gcgaccgcga gcagccgctg cacctgggct cggtcaagtc caacctcggc cacacccagg
3301 cggccgccgg tgttgccggc gtgatcaaga tggtgctggc gatgcgcgcg ggcacccttc
3361 cccgcactct gcacgcatcg gagcggtcga aggagatcga ctggtcatcc ggtgcgatca
3421 gcctgctcga cgagccggag ccgtggcccg ccggcgcgcg accgcgccgg gcgggggtct
3481 cgtcgttcgg catcagcggc accaacgcgc acgccatcat cgaggaagct ccgcaggtcg
3541 tcgaaggcga gcgggtcgag gccggcgacg tcgtggcgcc ctgggtgctt tcggcgagca
3601 gcgcggaagg tctgcgcgcc caggcggcgc ggctggccgc gcacctgcgc gagcaccccg
3661 gtcaggaccc gcgcgacatc gcgtactcgc tcgcgacggg acgggccgcg ctgccccacc
3721 gcgccgcctt cgcccccgtc gacgagtccg ccgcgctgcg cgtgctcgac ggtctcgcga
3781 cgggaaacgc cgacggtgcc gccgttggaa cgagccgggc gcagcagcgc gccgtcttcg
3841 tcttccccgg gcagggttgg cagtgggcgg gcatggccgt cgacctgctc gacacctccc
3901 cggttttcgc agccgcgttg cgcgagtgcg ccgacgcgct cgaaccgcat ctggacttcg
3961 aggtgatccc gttcctgcgc gcggaagccg cgaggcggga gcaggacgcg gcgctgtcga
4021 ccgagcgcgt ggacgtggtg cagcccgtga tgttcgcggt catggtctcg ctggcgtcga
4081 tgtggcgagc ccacggcgtc gagccggccg cggtcatcgg gcactcccag ggcgagatcg
4141 ccgccgcgtg cgtcgcgggc gcgctctcgc tggacgacgc cgcgcgcgtg gtcgcgctgc
4201 gcagccgcgt catcgccacc atgcccggga acaagggcat ggcctcgatc gccgctccgg
4261 ccggcgaagt ccgcgcgcga atcggtgacc gcgtcgagat cgccgccgtc aacggtccgc
4321 gctcggtggt ggtcgccggc gacagcgacg aactggaccg gctggtcgct tcctgcacca
4381 ccgagtgcat ccgcgccaag cggctggccg tggactacgc gtcgcactcc tcgcacgtcg
4441 agacgatccg agacgcactg cacgccgagc tgggagagga cttccacccg ctgccggggt
4501 tcgtgccctt cttctccacc gtcaccgggc gctggacgca gccggacgag ctcgacgccg
4561 ggtactggta ccggaacctg cgccgcaccg tgcggttcgc ggacgccgtc cgtgcgctcg
4621 ccgagcaggg atatcgcacg ttcctggagg tcagcgcgca cccgatcctc accgccgcga
4681 tcgaggagat cggcgacgga tcgggcgccg acctctccgc catccattcg ctgcgccgcg
4741 gtgacggcag cctcgcggac ttcggcgaag cgctctcccg cgcgttcgcc gccggtgtcg
4801 cggtggactg ggagtcggtg cacctgggca ccggagcacg ccgggtgccc ttgcccacct
4861 acccgttcca gcgcgagcgc gtctggctcg aaccgaagcc ggtggcgcgc cggtccaccg
4921 aggtcgacga ggtttccgcg ctgcgctacc gcatcgagtg gcggcccacc ggtgccggtg
4981 aacccgcccg gctcgacggc acctggctgg tggcgaagta cgccggaacc gcggacgaga
5041 cgagcaccgc ggctcgggag gccctggagt cggccggggc gcgggtccgc gaactggtcg
5101 tggacgcccg ctgcggtcgc gacgaactcg cggagcggct tcgttcggtc ggcgaggtgg
5161 caggagtgct gtccctgctc gcggtggacg aagcggagcc ggaggaggcg ccgctcgcgc
5221 tggcttcgct ggcggacacg ctcagcctcg tgcaggcgat ggtgtcggcc gaactcggat
5281 gtccgctgtg gacggtgacg gaaagcgccg tcgcgacggg gccgttcgaa cgcgtccgca
5341 acgccgccca cggcgccctg tggggcgtcg ggcgggtcat cgcgctggag aaccccgccg
5401 tgtggggcgg cctggtcgac gtgcccgcgg ggtcggtcgc cgagctggcc cggcacctcg
5461 cggcggtcgt gtccggcggc gccggtgagg accagctcgc gctgcgcgcc gacggggtgt
5521 acggacgccg gtgggtgcgc gcggcggccc cggcgaccga tgacgagtgg aaacccaccg
5581 gaaccgtgct ggtcaccggt ggcacgggcg gtgtcggcgg gcagatcgcg cgctggctcg
5641 cccggcgggg cgcgccccac ctgctgctgg tgagccgcag cgggccggac gcggacggcg
5701 ccggcgaact ggtcgccgag ctcgaggcgc tgggcgcccg gacgaccgtc gcggcctgcg
5761 acgtgaccga ccgcgagtcg gttcgcgagc tgctcggcgg catcggtgac gacgtcccgc
5821 tctcggcggt gttccacgcc gccgccacgc tcgacgacgg caccgtggac accctcaccg
5881 gcgagcgcat cgagcgggca agtcgcgcca aggtgctcgg cgcgcgcaac ctgcacgagc
5941 tgacgcgcga gctggacctg accgccttcg tgctgttctc gtccttcgcc tcggccttcg
6001 gcgcccccgg gctcggcggc tacgcgcccg gcaacgccta cctcgacggc ctcgcccagc
6061 agcggcggag cgacggactc cccgcgaccg ccgtggcgtg ggggacgtgg gcgggcagcg
6121 ggatggccga aggcgcggtg gccgaccgct tccgcaggca cggcgtcatc gagatgcctc
6181 ccgagacggc ctgccgggcg ttgcagaacg cgctggaccg cgccgaggtc tgcccgatcg
6241 tcatcgacgt caggtgggac cggttcctgc tcgcctacac cgcgcagcgc ccgaccaggc
6301 tcttcgacga gatcgacgac gcgcggcggg ctgcgccgca ggcgccggcc gaaccgcggg
6361 tgggcgcgct ggcgtcgctg cccgcgccgg agcgcgagga agcgctgttc gagctcgtgc
6421 gctcgcacgc ggccgccgtc ctcggccacg cctcggccga gcgggtgccc gccgaccagg
6481 ccttcgcgga actcggcgtc gactcgctgt cggcgcttga gctgcgcaac cggctcggcg
6541 ccgcgaccgg tgtccgcctg ccgacgacga ccgtcttcga ccaccccgac gtgcggacgc
6601 tggcggcgca cctggccgcc gaactcggcg gtgcgaccgg agccgagcag gcggcaccgg
6661 cgaccacggc ccccgtcgac gagccgatcg cgatcgtcgg catggcgtgc cggctgcccg
6721 gggaggtcga ctccccggag cggctgtggg agctgatcac ctcgggacgc gactccgcgg
6781 cggaggtccc cgatgaccgg ggctgggtcc ccgacgagct gatggcctcc gacgcggcgg
6841 gaacccgcgc ccacggcaac ttcatggcgg gcgccggtga cttcgacgcg gcgttcttcg
6901 ggatctcgcc gcgcgaggcg ctggcgatgg acccgcagca gcgccaggcg ctggagacga
6961 cgtgggaggc gctggaaagc gcgggcatcc caccggagac gttgcgcggc agcgacaccg
7021 gcgtgttcgt cggcatgtcc caccagggct acgcgaccgg gcgtccgcgc ccggaggacg
7081 gcgtcgacgg gtacctgctc accggcaaca ccgcgagcgt cgcgtcggga cgcatcgcct
7141 acgtgctggg gctggaaggt cccgcgctga cggtggacac ggcgtgttcg tcgtcgttgg
7201 tggcgttgca cacggcgtgt gggtcgttgc gtgacggtga ctgcggtctt gcggtggccg
7261 gtggtgtgtc ggtgatggcg ggtccggagg tgttcaccga gttctcccgc cagggcgcgc
7321 tctcgccgga cggccggtgc aagcccttct cggacgaggc cgacggattc ggtctcgggg
7381 agggttcggc gttcgtcgtg ctccagcggt tgtccgacgc caggcgggag ggccgccgcg
7441 tgctcggcgt ggtggccggg tccgcggtga accaggacgg cgcgagcaac gggctctccg
7501 ctccgagcgg cgtcgcgcag cagcgggtca tccgccgggc gtgggcgcgt gcggggatca
7561 cgggcgcgga tgtggccgtg gtggaggcgc atgggaccgg tacgcggctg ggcgatccgg
7621 tggaggcgtc ggcgttgctg gctacttacg gcaagtcgcg cgggtcgtcg ggcccggtgc
7681 tgctgggttc ggtgaagtcg aacatcggtc acgcgcaggc ggccgcgggt gtcgcgggcg
7741 tgatcaaggt gctgctcggc ctggaacgcg gtgtggtgcc cccgatgctg tgccggggcg
7801 agaggtcggg cctcatcgac tggtcctccg gcgagatcga gctcgcagac ggcgtgcggg
7861 agtggtcgcc cgccgcggac ggggtgcgcc gggcaggtgt gtcggcgttc ggggtgagcg
7921 ggacgaacgc gcacgtgatc atcgccgagc cgccggaacc ggagcccgtg ccgcaaccgc
7981 gacgcatgct gcccgcgacc ggggtggtgc cggtcgtgct gtcggccagg accggggcgg
8041 cgttgcgggc gcaggccggc aggctcgccg accacctcgc cgcgcatccc gggatcgcac
8101 cggccgacgt gagctggacg atggcgcggg cccgccagca cttcgaggag cgggccgcgg
8161 tgctcgccgc cgacaccgcc gaggccgtgc accggttgcg ggcggtggcc gacggcgcgg
8221 tggttcccgg tgttgtcacc ggcagtgcct ccgacggtgg ttcagtgttc gtcttccctg
8281 ggcagggtgc ccagtgggaa ggcatggcgc gggagttgtt gccggttccc gtcttcgccg
8341 agtcgatcgc cgagtgcgat gcggtgttgt cggaggtggc cggattctcg gtgtccgagg
8401 tgctggagcc acgtccggac gcgccgtcgc tggagcgggt cgacgtggtg cagccggtgc
8461 tgttcgcggt gatggtgtcg ctggcgcggt tgtggcgtgc ctgcggtgcc gttccttcgg
8521 ccgtcatagg gcactcgcag ggtgagatcg ccgccgcggt ggtggcggga gcgttgtcgc
8581 tggaggacgg catgcgcgtc gtcgcccgcc ggtcgagggc ggtgcgtgcg gtcgcgggcc
8641 gggggagcat gctctcggtg cgcggcggcc gctccgacgt cgagaagctg ctcgccgacg
8701 acagctggac cggcaggctg gaggtcgccg cggtcaacgg ccccgacgcc gtggtggtgg
8761 ccggtgacgc ccaggcggcg cgcgagttcc tggagtactg cgagggcgtg ggcatccgcg
8821 cccgcgcgat cccggtggac tacgcctcgc acaccgcgca cgtcgagccc gtgcgcgacg
8881 aactggtcca ggcgctggcc gggatcaccc cgcgacgggc cgaggtgccg ttcttctcca
8941 ccctgaccgg cgacttcctc gacggcaccg agctggacgc gggctactgg taccgcaacc
9001 tgcgtcaccc ggtggagttc cactccgccg tgcaggcgct gaccgaccag ggatacgcga
9061 cgttcatcga ggtcagcccg cacccggtgc tggcgtcgag cgtccaggag accctcgacg
9121 acgccgagtc ggacgcggcg gtgctcggga cgctggaacg cgacgcgggc gacgccgacc
9181 gcttcctcac ggcactcgcc gacgcgcaca cgcgcggtgt cgcggtcgac tgggaagcgg
9241 tgctcggccg ggccggactg gtcgacctgc cgggttatcc tttccagggc aagcggttct
9301 ggctgctgcc ggaccgcacc acccctcgtg acgagctcga cggctggttc taccgggtcg
9361 actggaccga ggtgccgcgc tccgaacctg ccgcgctgcg cggccgttgg ctcgtggtgg
9421 tgcccgaggg gcacgaggag gacggctgga ccgtcgaggt gcggtccgcg ctcgccgagg
9481 ccggcgccga accggaggtc acgcgcggcg tcggcgggct ggtcggtgac tgcgcgggcg
9541 tggtgtcgtt gctcgccctc gagggcgatg gtgcggtgca aacccttgtg ctggtgcggg
9601 aactcgacgc cgagggcatc gacgcgccac tgtggacggt caccttcggc gcggtcgacg
9661 cgggcagtcc ggtggcccgc ccggaccagg cgaagctgtg ggggctgggc caggtcgcgt
9721 ccctggaacg cgggccccgc tggaccggcc tcgtcgacct gccgcacatg ccggacccgg
9781 aactgcgagg ccgtctcacc gcggtgctgg ccggctcgga ggaccaggtc gcggtgcgcg
9841 ccgacgccgt gcgtgcgcgg cggctttccc ccgcccacgt caccgccacc tcggagtacg
9901 cggtgccggg cggcacaatc ctggtcaccg gtggcaccgc cggcctgggc gcggaggtgg
9961 cccggtggct cgccggtcgc ggcgccgaac acctcgcgct ggtcagcagg cgaggcccgg
10021 acaccgaggg cgtcggcgac ctgaccgccg agctgacccg gctcggcgcg cgggtgtcgg
10081 tgcacgcgtg cgacgtcagc agccgcgaac cggtgaggga actcgtgcac ggcctgatcg
10141 agcagggcga cgtcgtccgc ggtgtggtgc acgcggcggg actgccgcag caggtcgcga
10201 tcaacgacat ggacgaggcc gccttcgacg aggtggtcgc ggccaaggcc gggggcgcgg
10261 tgcacctgga cgagctgtgc tcggacgccg agctgttcct gctgttctcc tccggggccg
10321 gggtgtgggg aagcgcccgc cagggcgcct acgccgcggg caacgcgttc ctggacgcct
10381 tcgcccggca ccgccggggc cgcggcctgc ccgccacgtc ggtggcgtgg gggctgtggg
10441 cggcgggcgg catgaccggc gacgaggagg ccgtgtcgtt cctgcgcgag cgcggtgtgc
10501 gggcgatgcc cgtaccgcgc gccctcgccg ccctggacag ggtgctggcc tccggggaga
10561 cggcggtggt cgtgacggac gtggactggc ccgccttcgc cgagtcctac accgccgccc
10621 ggccccggcc gttgctcgac cgcatcgtca cgaccgcgcc gagcgagcgg gccggagaac
10681 cggagacgga gagcctgcgc gaccggctgg cgggtctgcc gcgtgccgag cggacggcgg
10741 agctggtgcg cctggtccgc accagcaccg cgaccgtgct gggccacgac gacccgaagg
10801 cggtgcgcgc gaccacgccg ttcaaggagc tcgggttcga ctcgctggcg gccgtccggc
10861 tgcgcaacct gctcaacgcg gccaccgggc tccgcctgcc gtcgacgctg gtcttcgacc
10921 acccgaacgc ctccgcggtc gccggtttcc tcgacgccga gctcggcacc gaggtccggg
10981 gggaggcgcc gtcggccctc gccgggctgg acgcgctgga aggcgccctg cccgaggtgc
11041 ccgcaaccga gcgggaagag ctggtacagc gcttggaacg gatgctcgcc gcgctacgcc
11101 cggtcgccca ggccgccgac gcctccggga ccggcgccaa cccgtccggc gacgacctgg
11161 gcgaggcggg cgtggacgaa ctgctcgaag cactcggccg ggagctcgac ggcgattga
//