GenBank-Updates@genbank.bio.net (05/02/91)
LOCUS SYNSU2718 2338 bp ds-DNA SYN 02-MAY-1991
DEFINITION Cloning vector pSU2718 DNA.
ACCESSION M64731
KEYWORDS .
SOURCE Cloning vector pSU2718 DNA.
ORGANISM Cloning vector
Artificial sequences; Cloning vehicles.
REFERENCE 1 (sites)
AUTHORS Martinez,E., Bartolome,B. and de la Cruz,F.
TITLE pACYC184-derived cloning vectors containing the multiple cloning
site and lacZ-alpha reporter gene of pUC8/9 and pUC18/19 plasmids
JOURNAL Gene 68, 159-162 (1988)
STANDARD full staff_review
REFERENCE 2 (sites)
AUTHORS Rose,R.E.
TITLE The nucleotide sequence of pACYC184
JOURNAL Nucleic Acids Res. 16, 355-355 (1988)
STANDARD full staff_review
REFERENCE 3 (bases 1 to 2338)
AUTHORS Chandler,M.S.
TITLE New shuttle vectors for Haemophilus influenzae and Escherichia
coli: P15A-derived plasmids replicate in H. influenzae Rd
JOURNAL Plasmid (1991) In press
STANDARD full automatic
FEATURES Location/Qualifiers
misc_feature 1..584
/function="Bases 1 to 584 of pACYC184"
CDS complement(219..1898)
/pseudo
/function="Chloramphenicol resistance peptide"
/codon_start=1896
misc_feature complement(584..1874)
/function="BanI-HaeII fragment of Tn9"
misc_feature 585..1029
/function="from pUC18"
misc_feature 585..1029
/function="HaeII fragment of pUC18 containing lacZ-alpha
and the MCS"
misc_feature 1030..1873
/function="bases 585 to 1428 of pACYC184"
misc_feature 1030..1873
/function="P15A origin of replication"
misc_feature 1874..2338
/function="bases 3780 to 4244 of pACYC184"
BASE COUNT 584 a 575 c 586 g 593 t
ORIGIN
1 gaattccgga tgagcattca tcaggcgggc aagaatgtga ataaaggccg gataaaactt
61 gtgcttattt ttctttacgg tctttaaaaa ggccgtaata tccagctgaa cggtctggtt
121 ataggtacat tgagcaactg actgaaatgc ctcaaaatgt tctttacgat gccattggga
181 tatatcaacg gtggtatatc cagtgatttt tttctccatt ttagcttcct tagctcctga
241 aaatctcgat aactcaaaaa atacgcccgg tagtgatctt atttcattat ggtgaaagtt
301 ggaacctctt acgtgccgat caacgtctca ttttcgccaa aagttggccc agggcttccc
361 ggtatcaaca gggacaccag gatttattta ttctgcgaag tgatcttccg tcacaggtat
421 ttattcggcg caaagtgcgt cgggtgatgc tgccaactta ctgatttagt gtatgatggt
481 gtttttgagg tgctccagtg gcttctgttt ctatcagctg tccctcctgt tcagctactg
541 acggggtggt gcgtaacggc aaaagcaccg ccggacatca gcgccattcg ccattcaggc
601 tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga
661 aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac
721 gttgtaaaac gacggccagt gccaagcttg catgcctgca ggtcgactct agaggatccc
781 cgggtaccga gctcgaattc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat
841 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
901 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
961 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
1021 attgggcgct agcggagtgt atactggctt actatgttgg cactgatgag ggtgtcagtg
1081 aagtgcttca tgtggcagga gaaaaaaggc tgcaccggtg cgtcagcaga atatgtgata
1141 caggatatat tccgcttcct cgctcactga ctcgctacgc tcggtcgttc gactgcggcg
1201 agcggaaatg gcttacgaac ggggcggaga tttcctggaa gatgccagga agatacttaa
1261 cagggaagtg agagggccgc ggcaaagccg tttttccata ggctccgccc ccctgacaag
1321 catcacgaaa tctgacgctc aaatcagtgg tggcgaaacc cgacaggact ataaagatac
1381 caggcgtttc ccctggcggc tccctcgtgc gctctcctgt tcctgccttt cggtttaccg
1441 gtgtcattcc gctgttatgg ccgcgtttgt ctcattccac gcctgacact cagttccggg
1501 taggcagttc gctccaagct ggactgtatg cacgaacccc ccgttcagtc cgaccgctgc
1561 gccttatccg gtaactatcg tcttgagtcc aacccggaaa gacatgcaaa agcaccactg
1621 gcagcagcca ctggtaattg atttagagga gttagtcttg aagtcatgcg ccggttaagg
1681 ctaaactgaa aggacaagtt ttggtgactg cgctcctcca agccagttac ctcggttcaa
1741 agagttggta gctcagagaa ccttcgaaaa accgccctgc aaggcggttt tttcgttttc
1801 agagcaagag attacgcgca gaccaaaacg atctcaagaa gatcatctta ttaatcagat
1861 aaaatatttc taggcaccaa taactgcctt aaaaaaatta cgccccgccc tgccactcat
1921 cgcagtactg ttgtaattca ttaagcattc tgccgacatg gaagccatca cagacggcat
1981 gatgaacctg aatcgccagc ggcatcagca ccttgtcgcc ttgcgtataa tatttgccca
2041 tggtgaaaac gggggcgaag aagttgtcca tattggccac gtttaaatca aaactggtga
2101 aactcaccca gggattggct gagacgaaaa acatattctc aataaaccct ttagggaaat
2161 aggccaggtt ttcaccgtaa cacgccacat cttgcgaata tatgtgtaga aactgccgga
2221 aatcgtcgtg gtattcactc cagagcgatg aaaacgtttc agtttgctca tggaaaacgg
2281 tgtaacaagg gtgaacacta tcccatatca ccagctcacc gtctttcatt gccatacg
//GenBank-Updates@genbank.bio.net (05/25/91)
LOCUS SYNSU2718 2338 bp ds-DNA Circular SYN 25-MAY-1991
DEFINITION Cloning vector pSU2718 DNA.
ACCESSION M64731
KEYWORDS chloramphenicol resistance protein; cloning vector.
SOURCE Cloning vector pSU2718 DNA.
ORGANISM Cloning vector
Artificial sequences; Cloning vehicles.
REFERENCE 1 (sites)
AUTHORS Martinez,E., Bartolome,B. and de la Cruz,F.
TITLE pACYC184-derived cloning vectors containing the multiple cloning
site and lacZ-alpha reporter gene of pUC8/9 and pUC18/19 plasmids
JOURNAL Gene 68, 159-162 (1988)
STANDARD full staff_review
REFERENCE 2 (sites)
AUTHORS Rose,R.E.
TITLE The nucleotide sequence of pACYC184
JOURNAL Nucleic Acids Res. 16, 355-355 (1988)
STANDARD full staff_review
REFERENCE 3 (bases 1 to 2338)
AUTHORS Chandler,M.S.
TITLE New shuttle vectors for Haemophilus influenzae and Escherichia
coli: P15A-derived plasmids replicate in H. influenzae Rd
JOURNAL Plasmid (1991) In press
STANDARD full automatic
FEATURES Location/Qualifiers
CDS join(complement(1..219),complement(1898..2338))
/function="Chloramphenicol resistance peptide"
/codon_start=219
misc_feature 1..584
/function="Bases 1 to 584 of pACYC184"
misc_feature complement(584..1874)
/function="BanI-HaeII fragment of Tn9"
misc_feature 585..1029
/function="from pUC18"
misc_feature 585..1029
/function="HaeII fragment of pUC18 containing lacZ-alpha
and the MCS"
misc_feature 1030..1873
/function="bases 585 to 1428 of pACYC184"
misc_feature 1030..1873
/function="P15A origin of replication"
misc_feature 1874..2338
/function="bases 3780 to 4244 of pACYC184"
BASE COUNT 584 a 575 c 586 g 593 t
ORIGIN
1 gaattccgga tgagcattca tcaggcgggc aagaatgtga ataaaggccg gataaaactt
61 gtgcttattt ttctttacgg tctttaaaaa ggccgtaata tccagctgaa cggtctggtt
121 ataggtacat tgagcaactg actgaaatgc ctcaaaatgt tctttacgat gccattggga
181 tatatcaacg gtggtatatc cagtgatttt tttctccatt ttagcttcct tagctcctga
241 aaatctcgat aactcaaaaa atacgcccgg tagtgatctt atttcattat ggtgaaagtt
301 ggaacctctt acgtgccgat caacgtctca ttttcgccaa aagttggccc agggcttccc
361 ggtatcaaca gggacaccag gatttattta ttctgcgaag tgatcttccg tcacaggtat
421 ttattcggcg caaagtgcgt cgggtgatgc tgccaactta ctgatttagt gtatgatggt
481 gtttttgagg tgctccagtg gcttctgttt ctatcagctg tccctcctgt tcagctactg
541 acggggtggt gcgtaacggc aaaagcaccg ccggacatca gcgccattcg ccattcaggc
601 tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga
661 aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac
721 gttgtaaaac gacggccagt gccaagcttg catgcctgca ggtcgactct agaggatccc
781 cgggtaccga gctcgaattc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat
841 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
901 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
961 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
1021 attgggcgct agcggagtgt atactggctt actatgttgg cactgatgag ggtgtcagtg
1081 aagtgcttca tgtggcagga gaaaaaaggc tgcaccggtg cgtcagcaga atatgtgata
1141 caggatatat tccgcttcct cgctcactga ctcgctacgc tcggtcgttc gactgcggcg
1201 agcggaaatg gcttacgaac ggggcggaga tttcctggaa gatgccagga agatacttaa
1261 cagggaagtg agagggccgc ggcaaagccg tttttccata ggctccgccc ccctgacaag
1321 catcacgaaa tctgacgctc aaatcagtgg tggcgaaacc cgacaggact ataaagatac
1381 caggcgtttc ccctggcggc tccctcgtgc gctctcctgt tcctgccttt cggtttaccg
1441 gtgtcattcc gctgttatgg ccgcgtttgt ctcattccac gcctgacact cagttccggg
1501 taggcagttc gctccaagct ggactgtatg cacgaacccc ccgttcagtc cgaccgctgc
1561 gccttatccg gtaactatcg tcttgagtcc aacccggaaa gacatgcaaa agcaccactg
1621 gcagcagcca ctggtaattg atttagagga gttagtcttg aagtcatgcg ccggttaagg
1681 ctaaactgaa aggacaagtt ttggtgactg cgctcctcca agccagttac ctcggttcaa
1741 agagttggta gctcagagaa ccttcgaaaa accgccctgc aaggcggttt tttcgttttc
1801 agagcaagag attacgcgca gaccaaaacg atctcaagaa gatcatctta ttaatcagat
1861 aaaatatttc taggcaccaa taactgcctt aaaaaaatta cgccccgccc tgccactcat
1921 cgcagtactg ttgtaattca ttaagcattc tgccgacatg gaagccatca cagacggcat
1981 gatgaacctg aatcgccagc ggcatcagca ccttgtcgcc ttgcgtataa tatttgccca
2041 tggtgaaaac gggggcgaag aagttgtcca tattggccac gtttaaatca aaactggtga
2101 aactcaccca gggattggct gagacgaaaa acatattctc aataaaccct ttagggaaat
2161 aggccaggtt ttcaccgtaa cacgccacat cttgcgaata tatgtgtaga aactgccgga
2221 aatcgtcgtg gtattcactc cagagcgatg aaaacgtttc agtttgctca tggaaaacgg
2281 tgtaacaagg gtgaacacta tcccatatca ccagctcacc gtctttcatt gccatacg
//