GenBank-Updates@genbank.bio.net (05/25/91)
LOCUS XELRN01 8153 bp ds-DNA VRT 25-MAY-1991
DEFINITION Xenopus laevis genes for 18S, 5.8S and 28S ribosomal RNAs
ACCESSION X02995 J01006 J01007 K01369 K01370 K01371 K01372 K01373 K01376
V01444 V01445 V01446 V01447 V01448 V01449 V01450 V01451 V01452
V01453 V01454 V01456 X00136 X01071
KEYWORDS 18S ribosomal RNA; 28S ribosomal RNA; 5.8S ribosomal RNA;
repetitive sequence; ribosomal RNA.
SOURCE Xenopus laevis DNA.
ORGANISM Xenopus laevis
Eukaryota; Animalia; Metazoa; Chordata; Vertebrata; Amphibia;
Lissamphibia; Anura; Archeobatrachia; Pipoidea; Pipidae.
REFERENCE 1 (sites)
AUTHORS Ford,P.J. and Mathieson,T.
TITLE The nucleotide sequences of 5.8-S ribosomal RNA from Xenopus laevis
and Xenopus borealis
JOURNAL Eur. J. Biochem. 87, 199-214 (1978)
STANDARD full staff_review
REFERENCE 2 (sites)
AUTHORS Boseley,P.G., Tuyns,A. and Birnstiel,M.L.
TITLE Mapping of the Xenopus laevis 5.8S rDNA by restriction and DNA
sequencing
JOURNAL Nucleic Acids Res. 5, 1121-1137 (1978)
STANDARD full staff_review
REFERENCE 3 (bases 1 to 24)
AUTHORS Boseley,P.G., Moss,T., Maechler,M., Portmann,R. and Birnstiel,M.L.
TITLE Sequence organization of the spacer DNA in a ribosomal gene unit of
Xenopus laevis
JOURNAL Cell 17, 19-31 (1979)
STANDARD full automatic
REFERENCE 4 (bases 7918 to 8153)
AUTHORS Sollner-Webb,B. and Reeder,R.H.
TITLE The nucleotide sequence of the initiation and termination sites for
ribosomal RNA transcription in X. laevis
JOURNAL Cell 18, 485-499 (1979)
STANDARD full automatic
REFERENCE 5 (sites)
AUTHORS Moss,T., Boseley,P.G. and Birnstiel,M.L.
TITLE more ribosomal spacer sequences from xenopus laevis
JOURNAL Nucleic Acids Res. 8, 467-485 (1980)
STANDARD full staff_review
REFERENCE 6 (sites)
AUTHORS Salim,M. and Maden,B.E.
TITLE Nucleotide sequence encoding the 5' end of Xenopus laevis 18S rRNA
JOURNAL Nucleic Acids Res. 8, 2871-2883 (1980)
STANDARD full staff_review
REFERENCE 7 (bases 2855 to 3953)
AUTHORS Hall,L.M. and Maden,B.E.
TITLE Nucleotide sequence through the 18s-28s intergene region of a
vertebrate ribosomal transcription unit
JOURNAL Nucleic Acids Res. 8, 5993-6005 (1980)
STANDARD full automatic
REFERENCE 8 (bases 1030 to 2854)
AUTHORS Salim,M. and Maden,B.E.
TITLE Nucleotide sequence of Xenopus laevis ribosomal RNA inferred from
gene sequence
JOURNAL Nature 291, 205-208 (1981)
STANDARD full automatic
REFERENCE 9 (sites)
AUTHORS Maden,B.E., Forbes,J.M., Stewart,M.A. and Eason,R.
TITLE 18S coding sequences in amplified ribosomal DNA from Xenopus laevis
oocytes are highly homogeneous, unmethylated, and lack major open
reading frames
JOURNAL EMBO J. 1, 597-601 (1982)
STANDARD full staff_review
REFERENCE 10 (bases 25 to 1029)
AUTHORS Maden,B.E., Moss,M. and Salim,M.
TITLE Nucleotide sequence of an external transcribed spacer in Xenopus
laevis rDNA: sequences flanking the 5' and 3' ends are
complementary
JOURNAL Nucleic Acids Res. 10, 2387-2398 (1982)
STANDARD full automatic
REFERENCE 11 (sites)
AUTHORS Stewart,M.A., Hall,L.M. and Maden,B.E.
TITLE Multiple heterogeneities in the transcribed spacers of ribosomal
DNA from Xenopus laevis
JOURNAL Nucleic Acids Res. 11, 629-646 (1983)
STANDARD full staff_review
REFERENCE 12 (bases 3954 to 7917)
AUTHORS Ware,V.C., Tague,B.W., Clark,C.G., Gourse,R.L., Brand,R.C. and
Gerbi,S.A.
TITLE Sequence analysis of 28S ribosomal DNA from the amphibian Xenopus
laevis
JOURNAL Nucleic Acids Res. 11, 7795-7817 (1983)
STANDARD full automatic
COMMENT This sequence was assembled from refs.1-6 above.The rRNA gene is
present as multiple tandem repeats. The non-transcribed region is
approximately 5kB long, and includes entries XLRNA3 and XLRNA6.
There is considerable variation between gene copies in all the
non-coding regions, as described in 1-7 and references therein.
From EMBL 26 entry XLRN01; dated 10-APR-1991.
FEATURES Location/Qualifiers
promoter 290..297
/note="oligo-T putative promoter"
precursor_RNA 317..7917
/note="rRNA precursor"
intron 317..1029
/note="external transcribed spacer"
rRNA 1030..2854
/note="18S rRNA"
intron 2855..3411
/note="internal transcribed spacer 1"
rRNA 3412..3573
/note="5.8S rRNA"
intron 3574..3835
/note="internal transcribed spacer 2"
rRNA 3836..7917
/note="28S rRNA"
BASE COUNT 1421 a 2677 c 2808 g 1247 t
ORIGIN
1 ctgcagcccc accgggagtt ccaggagctc gggcaggggg agccggctcg tcccccggca
61 ccggaggtcc ccgggccctt tggcgcccgt tttttcgcaa agtgcggcgc ccgcggggac
121 ttgctcggcc gggccgggcc ccggcggccc cggggccccg gggccctccc gcggaggccc
181 cgatgaggac ggattcgccc ggcccgcccc ggccggagtt ccgggagccc ggggagagga
241 gccggcggcc cggcctctcg ggccccccgc acgacgcctc catgctacgc ttttttggca
301 tgtgcgggca ggaaggtagg ggaagaccgg ccctcggcgc gacgggcgcc cgaaaaaagg
361 accggggcgt ttcccgcctc ggtccccggt ctgggaaggc tccgcggtcg agtctcgctc
421 cccggcccga tcgatctggc aacccgcgcc cgggcgggga gggccctctg cccggccgac
481 cccccggcgg ggcggccgct acacgggcag ggaggctccc tcccgcctcg ccccgggcgc
541 gacccccgga cccccccccg gtccggtccg ccgccgggcc cacccccccg gccgccccgg
601 cggccggggg gcgcccgcgc cccccccgtg agttcccccc gcaccgtccg accccggcag
661 gcgcccgaag aaggcccgga caggcgggga gcccgccccg ggggaccccg cttcccccgg
721 ccgcggactc ctctcccctg gcccgggccg agcaccccgt ttcgcccgac acccgcagag
781 cgagagagaa agacggaaag aaaggagagt aggccgcggg ccccgtcccg gccgccgcct
841 cccccccccc tccccggggg gggggagcgg caggccgggc ggggcccccg gcccggacgg
901 gagggcccgg gcgccgggag cgccgccgag gggacgggcc cgggtgacgc ctcagggcgc
961 cgacccgccg cccccccccc ccggccgccc ccgcgcccgc ccgcccgcgc cgggcccggg
1021 aaaggtggct acctggttga tcctgccagt agcatatgct tgtctcaaag attaagccat
1081 gcacgtgtaa gtacgcacgg ccggtacagt gaaactgcga atggctcatt aaatcagtta
1141 tggttccttt gatcgctcca tctgttactt ggataactgt ggtaattcta gagctaatac
1201 atgccgacga gcgctgaccc ccagggatgc gtgcatttat cagaccaaaa ccaatccggg
1261 gcccccgcgc cccggccgct ttggtgactc tagataacct cgggccgatc gcacgtcccc
1321 gtgacggcga cgatacattc ggatgtctgc cctatcaact ttcgatggta ctttctgcgc
1381 ctaccatggt gaccacgggt aacggggaat cagggttcga ttccggagag ggagcctgag
1441 aaacggctac cacatccaag gaaggcagca ggcgcgcaaa ttacccactc ccgacgcggg
1501 gaggtagtga cgaaaaataa caatacagga ctctttcgag gccctgtaat tggaatgagt
1561 acactttaaa tcctttaacg aggatctatt ggagggcaag tctggtgcca gcagccgcgg
1621 taattccagc tccaatagcg tatattaaag ttgctgcagt taaaaagctc gtagttggat
1681 cttgggatcg agctggcggt ccgccgcgag gcggctaccg cctgtcccag cccctgcctc
1741 tcggcgcctc cccgatgctc ttgactgagt gtcccggggg cccgaagcgt ttactttgaa
1801 aaaattagag tgttccaagc aggccgcgtc gcctggatac ttcagctagg aataatggaa
1861 taggactccg gttctatttt gttggttttc ggaactgggg ccatgattaa gagggacggc
1921 cgggggcatt cgtattgtgc cgctagaggt gaaattcttg gaccggcgca agacgaacca
1981 aagcgaaagc atttgccaag aatgttttca ttaatcaaga acgaaagtcg gaggttcgaa
2041 gacgatcaga taccgtcgta gttccgacca taaacgatgc cgactagcga tccggcggcg
2101 ttattcccat gacccgccga gcagcttccg ggaaaccaaa gtctttgggt tccgggggga
2161 gtatggttgc aaagctgaaa cttaaaggaa ttgacggaag ggcaccacca ggagtggagc
2221 ctgcggctta atttgactca acacgggaaa cctcacccgg cccggacacg gaaaggattg
2281 acagattgat agctctttct cgattctgtg ggtggtggtg catggccgtt cttagttggt
2341 ggagcgattt gtctggttaa ttccgataac gaacgagact cctccatgct aactagttac
2401 gcgacccccg gcggtcggcg tccaacttct tagagggaca agtggcgttc agccacacga
2461 gatcgagcaa taacaggtct gtgatgccct tagatgtccg gggctgcacg cgcgctacac
2521 tgaacggatc agcgtgtgtc taccctgcgc cgacaggtgc gggtaacccg ctgaaccccg
2581 ttcgtgatag ggatcgggga ttgcaattat ttcccatgaa cgaggaattc ccagtaagtg
2641 cgggtcataa gctcgcgttg attaagtccc tgccctttgt acacaccgcc cgtcgctact
2701 accgattgga tggtttagtg aggtcctcgg atcggccccg ccggggtcgg ccacggccct
2761 ggcggagcgc cgagaagacg atcaaacttg actatctaga ggaagtaaaa gtcgtaacaa
2821 ggtttccgta ggtgaacctg cggaaggatc attaacgaga cccccctcac ccggagagag
2881 ggaaggcgcc cgccgcaccc tccccgcgga gagagagaga gacgcccgcc ccggagcgga
2941 gaccgccccc cccccacggg gggggggcgg ccgccccgaa agggacgacg aggaaccccc
3001 agacggcccc ggcgaggggg cggcggcggc cccgggtcca ccccgggccc gcccgcccgc
3061 ctccccgccg cgggcccgcc cgggtaccta gccggggccg gggcgcgggg gctggcgcgg
3121 gagcggggcg gccccagggc cgtccggcct ccccgcgtcc gcctcccgcg acccgccccg
3181 ggcggttcga agaccccgcc cgccgggcgg cgggagggcc gggagggagc cggggagggg
3241 agggggggag gcggcggcga gcccggccgg gcgccgccgc cgcaggaccc ccgtccccgt
3301 cccgcgccgc ccccgccggc ccggggcgcc cgggccgcga ccgcctcagc ggcagcaccg
3361 gtagccctgc cgagaccgaa aaggaaaacc gaccgacgcg tcggcgagag ctcgcgactc
3421 ttagcggtgg atcactcggc tcgtgcgtcg atgaagaacg cagctagctg cgagaattag
3481 tgtgaattgc aggacacatt gatcatcgac acttcgaacg caccttgcgg ccccgggttc
3541 ctcccggggc cacgcctgtc tgagggtcgc tccgacgtcc atcgcccccg ccgggtcccg
3601 tcccggcgcg gaggcgcggc tggggccgtc gcaggggcgc gccgctcccc ttcgtccccc
3661 caaggccaga cccccggccc ggcgcccggg ccccggcccg gccggcggcg gctgtctgtg
3721 gatcccttca cggctgccgc cccggccggc cccccggggc cccggcccgc cggcgggagc
3781 gggcccggcc cccccccccg ggccgcggcc ccgcgccccc cccccccccc acgactcaga
3841 cctcagatca gacgcggcga cccgctgaat ttaagcatat tactaagcgg aggaaaagaa
3901 actaaccagg attcccccag taacggcgag tgaagaggga agagcccagc gccgaatccc
3961 gcgcccgccg ggcgcgggac gtgtggcgta cgggagaccg gacccccccg gcgcggctcg
4021 ggggcccaag tccttctgat cgaggcccag cccgcggacg gtgttaggcc ggtgggcggc
4081 ccccggcgcg gcgggacccg gtctcctcgg agtcgggttg tttgggaatg cagcccaaag
4141 cgggtggtaa actccatcta aggctaaata ccggcacgag accgatagcg gacaagtacc
4201 gtaaggaaag ttgaaaagaa ctttgaagag agagttcaag agggcgtgaa accgttaaga
4261 ggtaaacggg tggggccgtg cggtccgccc ggaggattca acccggcggg tcagcgccgc
4321 cgggaccggg ccactcggcg gacccccccg ccggccccct cccctcgccg ggagggcggt
4381 cgcgcggggg ggacgcggcc cgggcgcccg gcgccgcgtt tcctccgcgg cggtgcgcgc
4441 gccggctccg ggccgcgtgg gaaggcccag gggttcaggg gaaggtggcc ggccgccccc
4501 gcgcgcggct acagcccccc cccagcagca gcactcgccg tcgcccgggg ccgagggaga
4561 cgccggcctc cgcggtcctc ctccccggag cgcgtcccgc cgctcccccc cgggggggcg
4621 gcgcgcgggg cggggaaggg ggaaggggcc ccccgctccc ggcgcggctg tcaaccgggg
4681 cggactgccc ccagtgcgcc ccgtccgcgc cgcgccgccg aggcgggagg gccgccggga
4741 gccgccaggg tccgcggcga tgtcggtgtc ccacccgacc cgtcttgaaa cacggaccaa
4801 ggagtctaac gcgcgcgcga gtcggaggga ctctgcgcga aaccctgtgg cgcaatgaag
4861 gtgagggccg gggcgccccg gctgaggtgg gatcccgccg cccctccctc cgcccccccg
4921 ggggcggggg gggggcgccg gcgggccacc accggcccgt ctcgcccgcc ccgtcggggg
4981 gtgggcgtga gcgcgcgcga ttaggacccg aaagatggtg aactatgcct gggcaggcga
5041 agccagagga aactctggtg gaggtccgta gcggtcctga cgtgcaaatc ggtcgtccga
5101 cctggtatag gggcgaaaga cgaatcgaac catctagtag ctggttccct ccgaagtttc
5161 cctcaggata gctggcgctg tccgtcgcag ttttatccgg taaagcgaat gattagaggt
5221 cttggggccg aaatcgatct caacctattc tcaaacttta aatgggtaag aagcccggct
5281 cgctggcttg gagccggggc gtggaatgcg gcacgccata gtgggccact tttggtaagc
5341 agaactggcg ctgcgggatg aaccgaacgc cgggttaagg cgcccgatgc cgacgctcat
5401 cagaccccag aaaaggtgtt ggttgatata gacagcagga cggtggccat ggaagtcgga
5461 atccgctaag gagtgtgtaa caactcacct gccgaatcaa ctagccctga aaatggatgg
5521 cgctggagcg tcgggcccat acccggccgt cgccggcgct gggtcagtcc gcgggggcta
5581 ggccgcgact gagtaggagg gccccggcgg tgggcgcgga agcgcgcgcg agggcccggg
5641 tggagccgcc gcgggtgcag atcttggtgg tagtagcaaa tattcaaacg agaactttga
5701 aggccgaagt ggagaagggt tccatgtgaa cagcagttga acatgggtca gtcggtccta
5761 agagatgggc gagcgccgtt cggaagggac gggcgatggc ctccgtcgcc ctcggccgat
5821 cgaaagggag tcgggttcag atccccgaac ccggagtggc ggagacgggc gcccgcggcc
5881 ccccccgacg cctcgcggcg gcgggggggc gggggcgtcc agtgcggcga cgcgaccgat
5941 cccggagaag ccgggggagc ccgggagaag agttctcttt tctttgtgaa gggcagggcc
6001 gccctgaatg ggttcgcccc gagagagggg cccgcgccgt tggaaagcgt cgcggttccg
6061 gcggcgtccg gtgagcttct cgctggccct tgaaaatccg ggggagaggg tgtaaatctc
6121 tgcgccgggc cgtacccata tccgcagcag gtctccaagg tgaacagcct ctggcatgtt
6181 agaacaatgt aggtaaggga agtcggcaag tcagatccgt aacttcggga taaggattgg
6241 ctctaagggc tgggtcggtc gggctggggc gcgaagcggg gctgggccgc gccgcggctg
6301 gacgaaggcg cccccgtggc gctctttccc tcctcccgcc cccctctctc tccggccccc
6361 cctcgcgggg ggccgcgggg gcgggggggc gcggggggcc gggagcgccc ggcggcggcg
6421 actctggacg cgcgccgggc ccttcctgtg gatcgcccca gctgcggcgc gcgcctctcc
6481 cccgcgccgt ccccctcctg cgcctccccc cgtcagggga cggggcgcgg cgcggggcgg
6541 ccggggcggg cccggcctcg gccggcgcct agcagctgac ttagaactgg tgcggacagg
6601 gaatccgact gtttaattaa aacaaagcat cgcgaaggcc cgaggcgggt gttgacgcga
6661 tgtgatttct gcccagtgct ctgaatgtca aagtgaagaa attcaatgaa gcgcgggtaa
6721 acggcgggag taactatgac tctcttaagg tagccaaatg cctcgtcatc taattagtga
6781 cgcgcatgaa tggatgaacg agattcccac tgtccctacc tactatctag cgaaaccaca
6841 gccaagggaa cgggcttggc ggaatcagcg gggaaagaag accctgttga gcttgactct
6901 agtctgcaac tgtgaagaga catgagaggt gtaggataag tgggaggccc ccgcgctcgt
6961 cgcaaagggg cgccgccggt gaaataccac tactcttatc gttttttcac ttacccggtg
7021 aggcgggggc gagccccgag gggctctcgc ttctggaccc aagcgccggc ccccgcgccg
7081 ggcgcgaccc gctccgagga cagtggcagg tggggagttt gactggggcg gtacacctgt
7141 caaaccgtaa cgcaggtgtc ctaaggcgag ctcaggcgag ctacagaaac ctcccgtgga
7201 gcagaagggc aaaagctcgc ttgatcttga ttttcagtat gaatacagac cgtgaaacgc
7261 ggggcctcac gatccttctg actttttggg ttttaagcag gaggtgtcag aaaagttacc
7321 acagggataa ctggcttgtg gccggccaag cgttcatagc gacgtcgctt tttgatcctt
7381 cgatgtcggc tcttcctatc attgtgaagc agaattcacc aagcgttgga ttgttcaccc
7441 actaataggg aacgtgagct gggtttagac cgtcgtgaga caggttagtt ttaccctact
7501 gatgatgtgt tgtcgcaata gtaatcctgc tcagtacgag aggaaccgca ggttcagaca
7561 tttggtgtat gtgcttggct gaggagccaa tggggcgaag ctaccatctg tgggattatg
7621 actgaacgcc tctaagtcag aatcccccct aaacgtgacg ataccgcagc gccgcggagc
7681 ctcggtcggc ctcggattag ccggcgcccc ccccgggggg cgccggcggg cagagccgct
7741 cgcctcggga ccggagcgcg gacgaaaggg ggccgcctct ctcccggagc gcaccgcacg
7801 ttcgtgggga acctggtgct aaatcattcg tagacgacct gattctgggt cagggtttcg
7861 tgcgtagcag agcagctacc tcgctgcgat ctattgaaag tcatcccttg gccaagcttt
7921 tgtcggaagg agcaggccgg aagggcgccc ccgccgccgg ccggcgcgac gtcccgtccg
7981 ccctccccgg cctcccgccg cgctcccctt tccgcggggg ggagagagcg gcggcggggc
8041 gggggggagg cggccgggcg accgccgccg gcccggggac cgtcccgtcc cccggcctct
8101 cccgcaggga gggggccgaa ggcccggccc ccgcaccccc cctccctccc gcc
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