GenBank-Updates@genbank.bio.net (05/25/91)
LOCUS ECOPKG18G 1098 bp ds-DNA BCT 25-MAY-1991
DEFINITION E.coli pKG1800 vector gal promoter and galE gene
ACCESSION X51449
KEYWORDS gal operon; gale gene; promoter region.
SOURCE Escherichia coli DNA.
ORGANISM Escherichia coli
Prokaryota; Bacteria; Gracilicutes; Scotobacteria;
Facultatively anaerobic rods; Enterobacteriaceae.
REFERENCE 1 (bases 1 to 1098)
AUTHORS Bernardi,A.
JOURNAL Unpublished (1990)
STANDARD full automatic
REFERENCE 2 (bases 1 to 1098)
AUTHORS Bernardi,F. and Bernardi,A.
TITLE Completed sequence of PKG1800, a vector for determination of
transcription terminators
JOURNAL DNA Sequence 1, 147-151 (1990)
STANDARD full automatic
COMMENT *source: strain=K12; See <V00278> for sequence overlap.
From EMBL 26 entry ECPKG18G; dated 15-APR-1991.
FEATURES Location/Qualifiers
misc_feature 546..575
/note="CRP binding site"
promoter 555..569
/note="operator region external"
promoter 605..611
/note="CRP independent promoter"
promoter 610..616
/note="CRP dependent promoter"
CDS 649..1089
/note="truncated galE gene product (AA 1-146)"
/codon_start=649
promoter 667..681
/note="operator region internal"
BASE COUNT 289 a 270 c 261 g 278 t
ORIGIN
1 gaattccgcg tggtgaactc acgtactcag caagaagcgt atgtgtttgc accggctacg
61 ctgtccaaca tttactacgg tttcctcgcc gtaaacagcc gtttcaatgc tttcggtgat
121 ggtgtggcgc aactgggccg ctcgctggat gttgatgcca ataccaacgg tcaggtggtg
181 atccgtgata gcgccatcaa cgaaggtttt aacacggcta aaccgtgggc cgatgcggtg
241 atctctaatc gtccgtttgc gggtaatacc ggcagcgtag atgataacga cgaaatacag
301 cgcaatctga atgacactaa ctacaaccgc atgtgggaat acaataaccg cggcgtgggt
361 agtaaagtgg ttgcagaggc gaagaagtaa gagcaattaa ctatttgccg gacgcggcgt
421 aaacgcctta tccggcctac ggttcgacgc atgcaggcat gaaaccgcgt cttttttcag
481 ataaaaagcg caatcattca taaaccctct gttttataat cacttaatcg cgcataaaaa
541 acggctaaat tcttgtgtaa acgattccac taatttattc catgtcacac ttttcgcatc
601 tttgttatgc tatggttatt tcataccata agcctaatgg agcgaattat gagagttctg
661 gttaccggtg gtagcggtta cattggaagt catacctgtg tgcaattact gcaaaacggt
721 catgatgtca tcattcttga taacctctgt aacagtaagc gcagcgtact gcctgttatc
781 gagcgtttag gcggcaaaca tccaacgttt gttgaaggcg atattcgtaa cgaagcgttg
841 atgaccgaga tcctgcacga tcacgctatc gacaccgtga tccacttcgc cgggctgaaa
901 gccgtgggcg aatcggtaca aaaaccgctg gaatattacg acaacaatgt caacggcact
961 ctgcgcctga ttagcgccat gcgcgccgct aacgtcaaaa actttatttt tagctcctcc
1021 gccaccgttt atggcgatca gcccaaaatt ccatacgttg aaagcttact ccccatcccc
1081 gggcaataag ggcgctgc
//